4 results about "Plant sterol ester" patented technology

The object of this invention is to provide Labyrinthula microorganisms that have high sterol ester production capacity. [Solution] A Labyrinthull microorganism modified to have reduced or lost activity of sterol 24-C-methyltransferase (SMT1) compared to an unmodified strain, wherein the Labyrinthull microorganism is modified to express a modified diacylglycerol acyltransferase 2C (modified DGAT2C) gene, and the modified diacylglycerol acyltransferase 2C (modified DGAT2C) is modified to have a defect in presumed transmembrane regions 1 to 8 of the presumed transmembrane regions 1 to 12 in diacylglycerol acyltransferase 2C (DGAT2C).

The present application relates to the technical field of microbial feed additive, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the following steps: taking Lactobacillus plantarum and / or Enterococcus faecalis as a bacterial strain to activate and expand culture, taking agricultural and sideline product waste as a substrate to obtain mature fermentation liquor through deep liquid fermentation; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide to obtain a semi-dry granule core through granulation and low-temperature pre-drying; and coating the semi-dry granule core with a κ-carrageenan / konjac glucomannan / plant sterol ester composite gel, and drying and packaging to obtain a finished product.The double essential oil, chitosan and theabrownin jointly form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; the porous silicon dioxide actively maintains low water activity of the granule to prevent the lactic acid bacteria from recovering in advance; and the product has high viable count, strong heat and acid resistance, and excellent storage stability.

The present application relates to the technical field of health food and soft capsule preparation, in particular to a kind of full sea-buckthorn oil sterol ester soft capsule and low-temperature homogenization preparation method thereof.The content of the soft capsule is composed of sea-buckthorn seed oil 41.7%, phytosterol 33.3% and sea-buckthorn fruit oil 22% by weight percentage, wherein, sea-buckthorn fruit oil completely replaces soybean oil and chemical synthesis suspending agent, as functional excipient and physical stabilizer, when preparing, high-quality sea-buckthorn oil is extracted at 38±0.5 ℃ subcritical low temperature, and the raw and auxiliary materials are mixed at 38±0.5 ℃ constant temperature;then, pill is pressed at 22-24 ℃ environment, and the accurate temperature difference of 14-16 ℃ is used to stimulate micro-phase rearrangement and viscosity improvement of oil liquid.The present application realizes instantaneous physical homogenization and long-term stable non-layering of content under the condition of zero addition of stabilizer, and realizes efficient comprehensive utilization of sea-buckthorn resources.

The present application relates to a kind of low-density extracellular vesicles and its preparation method and application, the low-density extracellular vesicles is derived from CD4 + Chimeric antigen receptor T (CD4 + CAR-T) cell culture fluid, after obtaining supernatant by ultracentrifugation, again by ultrafiltration cut-off purification is obtained.The present application is first isolated and identified from CD4 + CAR-T cell a new type of low-density extracellular vesicles (LD-EVs), the subtype is missed in traditional EV separation process, and the cognition to EV diversity is widened.The characteristics of LD-EVs are rich in sterol ester (especially cholesterin ester) by lipidomics etc., which is related to its low-density characteristics and potential membrane stability, signal transmission function, provides target for functional research and engineering modification.