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Method for degumming with magnetic immobilized phospholipase A2

An enzyme immobilized and magnetic technology, applied in the direction of edible oil/fat, application, and fat production, can solve the problems of difficult repeated or continuous use, difficult separation and recovery, and difficult removal of non-hydratable phospholipids.

Inactive Publication Date: 2014-12-10
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention is aimed at the problems that the free enzyme is miscible in the reaction system, which is not only difficult to separate and recover, but also difficult to use repeatedly or continuously, and it is difficult to remove the non-hydratable phospholipids in the oil. 2 method for degumming

Method used

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Effect test

specific Embodiment approach 1

[0006] Specific embodiment 1: A magnetically immobilized PLA 2 The method of degumming is achieved through the following steps: 1. PLA 2 Immobilization: First, 1.0g of magnetic microspheres PVP-DB-171 (polyvinylpyrrolidone-vinyltrimethoxysilane) / SiO 2 / Fe 3 O 4 Soaked in 50mL phosphate buffer (0.1M, pH=7.0) for 24 hours, then magnetically separated, and then add the soaked carrier to a certain amount of phosphate buffer containing 0.2% phospholipase (0.1M , PH=7.0), while slowly stirring the reaction with a magnetic stirrer at 45°C for a period of time, and finally magnetic separation and washing the immobilized enzyme with phosphate buffer (0.1M, pH=7.0). The filtrate and washing liquid are combined to determine the content of enzyme protein, and the immobilized enzyme obtained is stored in a refrigerator at 4°C; 2. Magnetically immobilized PLA 2 For degumming: Take 200 g of soybean oil, preheat it to 55°C, mix the immobilized phospholipase with a certain amount of deionized wat...

specific Embodiment approach 2

[0007] Specific embodiment two: this embodiment is different from specific embodiment one in that in step two, the initial pH value in the oil phase is 4.0-11.0 for immobilization of PLA 2 The other steps of degumming soybean crude oil are the same as in the first embodiment.

specific Embodiment approach 3

[0008] Specific embodiment three: This embodiment is different from specific embodiment one in that in step two, the initial pH value in the oil phase is 4.0 to 11.0, and the reaction time is 20 to 120 min. 2 Degumming of soybean oil. The other steps are the same as in the first embodiment.

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Abstract

The invention provides a method for degumming with magnetic immobilized phospholipase A2. The method comprises the steps of adding magnetic immobilized PLA2 (phospholipase A2) into soybean raw oil for degumming, and recycling magnetic immobilized enzyme with a magnetic field after a reaction is finished. For determination of a phosphorus content after the degumming, oil yield and recycling times of immobilized PLA2, the obtained minimum phosphorus content after the degumming is 20ppm, and the relative activity of the immobilized enzyme keeps around 80% after the oil yield is increased by 1.2%, and magnetic immobilized PLA2 is continuously utilized for eight lots. After magnetic immobilized PLA2, phospholipid formation in degumming oil can be increased; separation of an oil phase and a gum phase is improved; formation of free fatty acid in the degumming oil can be controlled; and sterol can be re-esterified with free fatty acid to form sterol ester to become a part of edible oil. Therefore, physical refining is realized, and the yield of the degumming oil can be increased. The high quality degumming oil with a low phosphorus content can be produced, and the degumming oil contains higher-content functional plant sterol ester at the same time.

Description

technical field [0001] The invention relates to a magnetically immobilized phospholipase A 2 The method of degumming. Background technique [0002] Magnetic functional material is a new type of composite material developed in the 1970s, and it is one of the important basic materials in the field of modern science and technology. The main characteristics of magnetic polymer microspheres are small particle size, magnetic responsiveness and functional group characteristics. The small particle size is reflected in the small volume, so the specific surface area, functional group density and selective adsorption capacity are large; the magnetic response is that it can be separated and magnetically guided under the action of an external magnetic field; the functional group characteristic is that the coated polymer The material has a variety of reactive functional groups that can be linked to active substances. The development trend of magnetic functional materials is to intersec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00A23D9/04
Inventor 于殿宇屈岩峰邹小雨王立琦江连洲
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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