1286 results about "De esterification" patented technology

The invention discloses a photo-thermal aging resistant polyester composite material and a preparation method thereof, a modified monomer, a modified filler, benzophenone, terephthalic acid and neopentyl glycol are subjected to esterification condensation polymerization to prepare the composite material, and the modified monomer is added, so that the prepared polyester molecule is of a branched structure; the side chain of a polyester molecule and the surface of the modified filler both contain unsaturated double bonds which can react after being irradiated by ultraviolet light, so that the number of crosslinking points of the composite material is increased, the crosslinking density is further improved, and the anti-aging effect of the material is further improved; the modified filler can improve the high-temperature-resistant effect of the composite material, the modified filler is matched with the hindered phenol structure of the side chain to further improve the heat aging-resistant effect of the composite material, hydroxyl and ketone groups on the modified filler form intramolecular hydrogen bonds, light energy can be converted into heat energy to be released, and then the light aging-resistant effect of the composite material is improved.

The invention provides modified acidic polysaccharide of lithocarpus litseifolius. The modified acidic polysaccharide is prepared from natural acidic polysaccharide of lithocarpus litseifolius through a chemical method or an enzyme method. Every 100 mg of the modified acidic polysaccharide of lithocarpus litseifolius contains 89.75 mg + / -1.04 mg to 90.67 mg + / -1.46 mg of total polysaccharide. The total uronic acid content is 23.70 mg + / -1.58 mg to 71.74 mg + / -2.07 mg; total bound phenol: 2.06 mg of GAE + / -0.06 mg of GAE-4.56 mg of GAE + / -0.04 mg of GAE (equivalent weight of gallic acid); the esterification degree is 3.72% + / -0.90% to 34.98% + / -0.62%; the molecular weight is (0.562 + / -0.012) * 10 < 4 > Da-(4.268 + / -0.055) * 10 < 4 > Da; the proportion of the galacturonic acid glycan (HG) is 15.28% to 65.02%; the ratio of the type I rhamnogalacturonan (RG-I) is 28.47% to 72.37%; and the length of a side chain is 0.44 to 10.31. The invention further provides a preparation method and application of the lithocarpus litseifolius modified acidic polysaccharide. The lithocarpus litseifolius modified acidic polysaccharide prepared by the invention has remarkable immunoregulatory activity, and especially has better immunocompetence when being used for removing arabinose side chains and reducing galactose side chains.

The present invention relates to the field of food technology, and in particular to a method for preparing diglyceride and its application. The method comprises the following steps: firstly hydrolyzing the oil and fat under the action of a first lipase, adding a solubilizing agent, and obtaining a hydrolyzate; then mixing the hydrolyzate with glycerol, and performing an esterification reaction with a second lipase; wherein the first lipase is prepared by compounding lipase Lipozyme RM and Antarctic Candida lipase B. The present invention can specifically synthesize 1,3-diglyceride that is beneficial to the cardiovascular system of the body, and has a high yield and good application prospects.

The invention discloses a Candida antarctica lipase B mutant with high transesterification activity and application thereof. The nucleotide sequence of the Candida antarctica lipase B mutant is shown as SEQ ID NO.1, and the amino acid sequence of the expression protein of the Candida antarctica lipase B mutant is shown as SEQ ID NO.2. The invention further discloses a preparation method of the Candida antarctica lipase B mutant. Compared with an original enzyme, the mutant has higher fat hydrolysis activity and transesterification activity, the specific enzyme activity of the fat hydrolysis activity reaches 480.39 U / mg, the specific enzyme activity of the transesterification activity reaches 40.67 U / mg, the biodiesel conversion rate at the temperature of 30 DEG C can reach 92%, low-temperature high catalytic activity is shown, and the mutant can be used for enzymatic preparation of biodiesel and has potential industrial application value.

The invention belongs to antibacterial materials, and particularly relates to antibacterial modified waterborne polyurethane and a preparation method and application thereof.The preparation method of the antibacterial modified waterborne polyurethane comprises the following steps that polyol and diisocyanate are mixed and subjected to a prepolymerization reaction, and a polyurethane prepolymer is obtained; mixing the polyurethane prepolymer and a chain extender, and carrying out chain extension reaction; in the chain extension reaction, adding the graft and carrying out esterification reaction to obtain a first intermediate; mixing the first intermediate with triethylamine and carrying out neutralization reaction to obtain a second intermediate; and emulsifying the second intermediate in deionized water to obtain the antibacterial modified waterborne polyurethane. Compared with the prior art, the antibacterial waterborne polyurethane has the advantages that the problems of poor intermiscibility, low durability and the like in physical addition and the problems of limited binding objects and weak antibacterial effect in covalent binding in the antibacterial waterborne polyurethane in the prior art are solved. According to the scheme, arginine and chitosan are grafted to a molecular chain of waterborne polyurethane, so that the waterborne polyurethane is endowed with a synergistic antibacterial property.

The invention discloses a synthesis method of a non-silicon defoaming agent and application of the non-silicon defoaming agent in the field of scandium extraction in hydrometallurgy. The defoaming agent is synthesized by a component A, a component B and a component C through a two-step reaction, the synthesis method comprises the following specific synthesis steps: 1) carrying out esterification reaction on an amino acid substance A and a fatty alcohol substance B to generate an amino ester substance D; and 2) carrying out substitution reaction on the amino ester substance D and a triazine compound C to obtain the non-silicon defoaming agent. The defoaming agent disclosed by the invention is wide in application range, can be applied to links such as a laterite-nickel ore hydrometallurgy front-end scandium extraction method and an MHP hydrometallurgy scandium impurity removal process, is added as an auxiliary agent in the extraction process, and is used for reducing the surface tension of liquid, preventing the formation and enrichment of a third-phase substance and reducing the occurrence of an emulsification phenomenon; the process stability and the output quality are improved, and the method has important industrial application value.

The invention discloses multifunctional acetylene carbon black as well as a preparation method and application thereof. The preparation method comprises the following steps: blending triethylenetetramine hexaacetic acid and acetylene carbon black in a mass ratio of (0.05-0.5): 100, and then carrying out esterification reaction under a vacuum condition, thereby obtaining the multifunctional acetylene carbon black. The preparation method comprises the following steps: modifying acetylene carbon black by adopting triethylenetetramine hexaacetic acid, and carrying out esterification reaction on triethylenetetramine hexaacetic acid with six carboxyl functional groups and acetylene carbon black with a hydroxyl functional group, so that the acetylene carbon black is modified, and the acetylene carbon black becomes multifunctional acetylene carbon black. The acetylene carbon black modified with a large number of carboxyl groups and amide groups on the semiconductive wrapping tape form rich hydrogen bonds, so that the compatibility of the acetylene carbon black and the semiconductive wrapping tape is improved.

The invention relates to the technical field of glycerol triacetate preparation, in particular to an esterification phase splitter for preparing glycerol triacetate, which comprises a tower tray arranged in a rectifying tower, umbrella-shaped covers are uniformly distributed on the upper surface of the tower tray, and the tower tray comprises a movable tray, a fixed tray, a riser and an adjusting mechanism. By optimizing the structure of the umbrella-shaped cover and the design of the adjusting mechanism, the problems of a viscous layer and entrainment caused by high viscosity of glycerol triacetate are solved, by reducing the diameter of the bottom of the umbrella-shaped cover and reducing the comprehensive height of the overflow weir, the liquid flowing space is enlarged, the liquid retention time is shortened, and by forming an air inlet groove and an adjusting hydraulic oil cylinder, the liquid retention time is shortened. The gas-phase flow area is increased, gas-liquid contact is optimized, entrainment is reduced, through adjustment of the hydraulic oil cylinder, adaptation to the requirements of different reaction stages is achieved, the height of the downcomer is increased, and the flooding risk is reduced.

The production equipment comprises a slurry tank, an esterification reactor, a multi-stage polycondensation tower and an esterification separation tower which are sequentially connected, the esterification separation tower is further circularly connected with the esterification reactor, the slurry tank is used for feeding alcohol and acid, the esterification reactor is used for carrying out esterification reaction, and the multi-stage polycondensation tower is connected with the esterification separation tower. The multi-stage polycondensation tower comprises multi-stage tower sections, the pressure of the multi-stage tower sections is gradually reduced from the feeding end to the discharging end, the multi-stage polycondensation tower is used for carrying out polycondensation reaction on esterification materials from the esterification reactor, polycondensation products generated by the multi-stage polycondensation tower are discharged, and the esterification separation tower is used for separating water, alcohol and small molecular substances in polycondensation condensate; and alcohol separated by the esterification separation tower flows back to the esterification reactor. The device can be used for continuous production of low-molecular-weight polyester, and can reduce equipment investment and separation energy consumption and reduce energy consumption and cost while improving efficiency and yield.

The invention belongs to the technical field of microbial fermentation, and discloses saccharomycopsis fibuligera and application thereof in rice wine. The saccharomycopsis fibuligera strain disclosed by the invention is Saccharomycopsis fibuligera ZB440, and the preservation number of the saccharomycopsis fibuligera strain is GDMCC No: 64395. The saccharomycopsis fibuligera has the characteristics of high temperature resistance, acid resistance, high alcohol resistance, high saccharifying enzyme activity, high esterifying enzyme activity and high alcohol yield. The method can be applied to rice wine fermentation, and the flavor substances of esters, alcohols, aldehydes and ketones can be obviously improved.

The invention discloses a method and equipment for detecting oxidized low-density lipoprotein based on immunoturbidimetry, and the method comprises the following steps: preparing a macroinitiator by active esterification reaction, and preparing a block graft copolymer by atom transfer radical polymerization and radical grafting reaction; the preparation method comprises the following steps: carrying out surface modification on latex microspheres with different particle sizes by using a grafted copolymer, and then respectively coupling the microspheres with different particle sizes with different OxLDL monoclonal antibodies so as to prepare a double-particle-size latex microsphere marker; and mixing the serum sample with the double-particle-size latex microsphere marker, measuring an absorbance value, and calculating the concentration of the oxidized low-density lipoprotein in the serum sample according to a standard curve established on the basis of the standard substance solution. According to the scheme, the stability of the latex microsphere marker can be enhanced, and the binding efficiency of the latex microsphere marker and ox-LDL can be improved.

The invention provides a bio-based 1, 4-butanediol composition as well as a preparation method and application thereof, and the bio-based 1, 4-butanediol composition comprises bio-based 1, 4-butanediol, 4-hydroxybutyric acid-(4-hydroxyl) butyl ester and bis (4-hydroxyl) dibutyl ether. The total mass content of 4-hydroxybutyric acid-(4-hydroxyl) butyl ester and bis (4-hydroxyl) dibutyl ether in the bio-based 1, 4-butanediol composition is 51 to 220 ppm. The bio-based 1, 4-butanediol composition has low hygroscopicity, and can maintain low water content after long-term storage; meanwhile, when the bio-based 1, 4-butanediol composition is used for polyester synthesis, the esterification time can be shortened, the reaction efficiency can be improved, side reactions can be reduced, and a polyester product with better damp-heat resistance stability can be obtained.

The invention belongs to the technical field of grease preparation, and particularly relates to a preparation method and application of diglyceride oil with a weight reducing effect. Comprising the following steps: firstly, adding grease into a basic nutrient solution, adding strains for fermentation, and filtering to obtain a glycolysis product; adding glycerol into the glycolysis product, carrying out lipase esterification reaction, and carrying out centrifugal separation to obtain an oil phase; the method comprises the following steps: degrading triglyceride by adopting dominant strain fermentation, then adding glycerol, and carrying out esterification reaction under the action of dominant compound lipase to obtain the diglyceride oil. According to the method, the 1, 3-diglyceride can be selectively generated, the proportion of dominant grease of the 1, 3-diglyceride can be increased, and research shows that the prepared diglyceride oil has good weight reduction and fat reduction effects and has a good application prospect.

The invention relates to the technical field of liquid chromatography, and discloses a mixed product liquid chromatography comprehensive analysis method which comprises the following steps: S1, mixing products: respectively mixing aldehyde compounds and ketone compounds with different mobile phases, and then injecting into an injection chromatographic system; s2, liquid chromatographic analysis: separating and analyzing a mixed product by using a liquid chromatograph; s3, performing qualitative analysis; on the basis of liquid chromatographic analysis, each peak in a chromatogram is identified. The method aims at qualitative and quantitative analysis of main substances (groups) in an organic reaction mixed product system; the method is suitable for analyzing the relative percentage content of products related to organic chemical reactions such as raw materials (nonylphenol) related to a core production process, nonylphenol formylation reaction, nonylphenol esterification reaction, phenolic ester Fris rearrangement reaction and the like, and samples in a refining process; the influence of absorption peaks and chromatographic line oscillation of other non-target substances on the content analysis result can be effectively avoided.

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention belongs to the technical field of compound synthesis, and particularly relates to a synthesis method of AEA-AEA. Triethylene glycol is adopted as a starting material, in the first step, p-toluenesulfonate with high leaving property is formed through TsCl, then nucleophilic substitution is performed, and a product 2 is obtained; the preparation method comprises the following steps: preparing a product 3, oxidizing by adopting TEMPO capable of being industrially produced, removing Pht through quantitative hydrazine hydrate to obtain a product 4, esterifying and extracting to obtain a product 5, acylating through the product 3 to obtain amide, washing, extracting and pulping to obtain an esterified product 6; and finally, quantitatively removing Pht, hydrolyzing and pulping to obtain a pure product. According to the method, complex operation steps of using metal hydride and alkylating column passing are avoided, process production can be realized, and the product yield and purity are relatively high.

The invention relates to the technical field of polymer compositions, and particularly discloses a degradable bio-based food packaging material and a preparation method thereof.The degradable bio-based food packaging material is prepared from polylactic acid, ITA esterified bacterial cellulose, CSL-ITA esterified bacterial cellulose, polycaprolactone glycol, acetyl tributyl citrate, a photoinitiator and tocopherol; the preparation method comprises the following steps: esterifying bacterial cellulose hydrogel with itaconic anhydride, grafting with sodium copper chlorophyllin, dehydrating with gradient ethanol, and drying with supercritical CO2 to obtain modified powder; according to the preparation method, the interfacial compatibility of the bacterial cellulose and the polylactic acid is enhanced through ITA esterification, the illumination antibacterial function is given by utilizing CSL grafting, the polycaprolactone glycol and the acetyl tributyl citrate are synergistically used for toughening and improving the brittleness, the photoinitiator is added, the photoinitiator is added for mixing, and ultraviolet crosslinking curing is performed after film forming. The tocopherol protects the stability of the antibacterial structure of the CSL, the water resistance is improved through photo-crosslinking, and the material is degradable and meets the food packaging requirement.

The invention relates to the field of ecological restoration, in particular to a degradable material for mine ecological restoration and a mine ecological restoration method. The material is prepared from the following raw materials in parts by weight: 30 to 50 parts of polycaprolactone, 10 to 20 parts of gamma-polyglutamate, 5 to 10 parts of a microbial agent, 20 to 30 parts of bentonite and 10 to 20 parts of pure wood fiber. The repairing method is realized based on the material. The degradable material is prepared from alcohol derivatives and gamma-polyglutamic acid through esterification cross-linking modification, the hydrophilicity of the material is reduced by introducing ester bonds, and the hydrophobicity of the material is improved; hydroxyl in the geniposide cross-linking agent reacts with active sites on gamma-polyglutamic acid and alcohol derivatives to form a complex three-dimensional network structure, so that the physical properties, such as hydrophobicity, mechanical properties and degradability, of the material are remarkably improved. The method can restore the ecology of a mine, degrade organic pollutants of soil and adsorb and solidify heavy metal ions, and has a wide application prospect.

The invention provides a method for preparing diglyceride oil by a solventless enzymatic method, which comprises the following steps: S1, mixing saturated fatty acid A and saturated fatty acid B to prepare a liquid eutectic mixture; s2, mixing the eutectic mixture, glycerol and lipase in a reaction system, and carrying out esterification reaction to obtain diglyceride; s3, separating the prepared diglyceride, adding the saturated fatty acid A and / or the saturated fatty acid B into the reaction system again to form a liquid eutectic mixture again, and repeating the step 2; wherein in the step S1, the saturated fatty acid A and the saturated fatty acid B are selected from C10-C20 alkanoic acid, in the step S2, the mass ratio of the eutectic mixture to the glycerol is 1: (1-5), and the temperature of the esterification reaction is 35-78 DEG C. The method solves the problem that the high-melting-point saturated fatty acid is difficult to utilize in the existing technology for preparing the diglyceride oil by the solvent-free enzymatic method.

The invention relates to the technical field of corrosion inhibitors, and discloses a preparation method of a modified imidazoline corrosion inhibitor, which comprises the following steps: carrying out quaternization reaction on tertiary amine group in an imidazoline intermediate and chlorine in benzyl chloride to generate quaternary ammonium salt, introducing hydroxyl, and continuing to carry out esterification reaction with boric acid group in 4-aminophenylboronic acid to obtain the modified imidazoline corrosion inhibitor. The modified imidazoline corrosion inhibitor is prepared by the following steps of: firstly, introducing amino groups, then carrying out amidation reaction with acyl chloride groups in 4-vinyl benzoyl chloride, introducing alkenyl groups, and finally carrying out click reaction with sulfydryl groups in DSB-TA, so as to generate the modified imidazoline corrosion inhibitor. The modified imidazoline corrosion inhibitor disclosed by the invention has a relatively good corrosion inhibition effect.

According to the high-flux chlorine-resistant and acid-alkali-resistant reverse osmosis composite membrane and the preparation method thereof, a porous supporting layer is sequentially immersed into a first water-phase solution, an organic-phase solution, a second water-phase solution and a third water-phase solution, ester bonds are formed through esterification reaction of water-soluble monomers, chlorine attack sites do not exist on the ester bonds, the chlorine resistance can be effectively improved, and the reverse osmosis composite membrane can be used for preparing the high-flux chlorine-resistant and acid-alkali-resistant reverse osmosis composite membrane. The reverse osmosis membrane with high flux and high desalination rate is obtained; the composite membrane is subjected to surface grafting modification, and the reverse osmosis active layer is covered with the protective layer, so that the chlorine resistance, acid resistance and alkali resistance of the membrane are greatly improved, and the service life of the membrane is prolonged; the preparation method has the characteristics of simplicity, convenience in operation, cheap and easily available raw materials, large-scale industrial production and the like, and can be widely applied to brackish water treatment and seawater desalination.

The invention discloses a preparation method of a high-boron filling type dynamic crosslinking organic silicon elastomer. The high-boron filling type dynamic crosslinking organic silicon elastomer is prepared from the following raw materials: 100 parts of vinyl silicone rubber, 0.1-10 parts of a thermal initiator, 1-20 parts of sulfydryl-terminated boric acid ester and 1-50 parts of polyborosiloxane gel PBS. The preparation method comprises the following steps: firstly, preparing polyborosiloxane (PBS) gel by utilizing esterification reaction; secondly, synthesizing sulfydryl-terminated boric acid ester, and preparing a high-boron filling type dynamic crosslinking organic silicon elastomer by utilizing a mixing reactor; and finally, an organic silicon sample piece is processed by compression molding. The invention develops a method which is simple to operate and can be used for preparing the high-boron filling type dynamic crosslinking organic silicon elastomer in batches, and the obtained organic silicon elastomer has the advantages of sensitive strain rate response, good metal adhesion, good heat resistance and repeated thermoplastic processing.

The invention relates to a preparation method and application of a wall coating type enzyme-loaded microchannel reactor. The method comprises the following steps: mixing tetramethyl orthosilicate, methyltrimethoxysilane and methanol to obtain a solution A; deionized water, polyethylene glycol, a sodium fluoride aqueous solution and a dialyzed lipase solution are mixed to obtain a solution B, the solution B and the solution B are mixed for 3-10 minutes in an ice bath environment to obtain a solution C, the solution C is introduced into the pretreated microchannel through an injection pump, after incubation is conducted for 10-60 minutes, nitrogen is adopted for purging, redundant sol-gel is removed, and then the solution C is put into a drying oven to be dried; the catalyst is used for hydrolysis reaction of grease and various ester exchange and esterification reactions. The method can be carried out in a mild environment, and has higher conversion rate and shorter reaction time.

The invention relates to the technical field of biocatalytic synthesis of fatty glyceride, in particular to a flaky zeolite immobilized Candida antarctica lipase B catalyst and a method for preparing medium-chain glyceride through catalytic esterification of the flaky zeolite immobilized Candida antarctica lipase B catalyst. The flaky zeolite is flaky silica-alumina zeolite ZSM-5-P, and the loading capacity of the candida antarctica lipase B is 9-12% of the mass of the flaky zeolite. The candida antarctica lipase B is loaded and fixed in the flaky zeolite to prepare the solid catalyst, and the catalyst is a solid enzyme biocatalyst, is small in usage amount, high in catalytic efficiency and recyclable, is used for preparing the medium-chain glyceride, is high in efficiency, low in cost, simple in process, mild in condition, safe and environment-friendly, and has a wide application prospect.

The invention discloses a functional intermediate for polycarboxylic acid synthesis modification and a preparation process thereof, the intermediate is a copolymer containing a phosphate group, an acrylate group and a hyperbranched polyether polyol chain segment, the molar ratio of all structural units is 1: (1-3): (0.5-2), and the preparation method comprises the following steps: esterification reaction; carrying out phosphorylation reaction; and carrying out gradient vacuum to remove small molecules. The intermediate is high in content of effective components, the viscosity is 500-2000mPa. S, the dispersing performance and the slump loss resistance of the polycarboxylate superplasticizer are remarkably improved through the synergistic effect of phosphate tackifying, acrylate dispersing and hyperbranched compatibilizing, the intermediate is particularly suitable for concrete construction under complex gravel geological conditions, the doping amount is reduced compared with that of traditional hydroxyethyl acrylate, and the service life of the polycarboxylate superplasticizer is prolonged. And the slump of the mother liquor can be kept lossless for a long time.

The invention discloses diglyceride prepared by an enzymic method as well as a preparation method and application thereof, and belongs to the technical field of deep processing of grease. The method comprises the following steps: adding fatty acid into a choline chloride-glycerol-urea ternary eutectic solvent, uniformly mixing, and then adding candida antarctica lipase B for esterification reaction to obtain a reaction mixture; separating the reaction mixture, and recovering an upper-layer oil phase; and separating and purifying the upper-layer oil phase to obtain the diglyceride. According to the method, the reaction rate is high, the selectivity of the diglyceride in the esterification process is high, the yield of the diglyceride can reach 65% or above by reacting for 1 h, the selectivity of the diglyceride is 75% or above, and the purity of the separated and purified diglyceride is 90% or above; in addition, the method is simple to operate, and the reaction product and the eutectic solvent can be separated through simple centrifugation after the reaction is finished; in addition, the monoglyceride content in the product is low, and the subsequent separation and purification difficulty is greatly reduced.

The invention relates to the technical field of biomedicine, in particular to a cross-linked modified artificial valve and a preparation method and application thereof. The preparation method comprises the following steps: firstly, preparing a novel compound POSS-PEG-NHS, and carrying out esterification reaction on a-NHS group of the compound and a-NH2 group on the surface of the decellularized porcine valve to realize covalent binding; then, an inherent reducing agent glutathione in an organism is added, and the cross-linked modified artificial valve PPN-GSH-AV is obtained. The valve shows excellent cell safety and blood compatibility, and has obvious advantages compared with a traditional glutaraldehyde cross-linked valve. In-vivo experiments show that the PPN-GSH-AV has high biological safety and histocompatibility, and can effectively reduce immunological rejection. In addition, the valve can relieve oxidative stress of macrophages and promote M2 type transformation, so that inflammatory response is relieved. In clinical application, the PPN-GSH-AV shows a good anti-inflammatory effect and excellent anti-calcification performance, in-vitro pulsating flow test results meet international standards, and the PPN-GSH-AV has clinical transformation potential and wide application prospects.

The invention belongs to the technical field of food and chemical industry, and particularly relates to a gas-liquid-solid three-phase fluidized bed enzyme reactor and a synchronous preparation method of grease enzyme method deacidification and diglyceride by using the same. According to the method, the gas phase of the three-phase fluidized bed is high-purity dry inert gas, the liquid phase is a uniform solution formed by mixing reaction substrate high-acid-value oil and an acyl acceptor, and the solid phase is immobilized enzyme. The method comprises the following steps: mixing high-acid-value oil and an acyl acceptor to prepare a raw material solution, preheating a feeding tank to a reaction temperature, adding the raw material solution into a gas-liquid-solid three-phase fluidized bed reactor, and carrying out esterification and / or transesterification under the bubbling action of inert gas to realize the deacidification of the high-acid-value oil and the synchronous preparation of the functional lipid. The mixture of the deacidified grease and the functional lipid is obtained. The method has the characteristics of high catalytic efficiency, easiness in separation of the product from enzyme, high nutritional value of the product, no need of adding an organic solvent, difficulty in oxidation of grease and suitability for large-scale production.

The invention discloses a method for separating boron isotopes through extraction-back extraction. The method comprises the following steps: 1, carrying out esterification reaction on a boron-containing water phase and a fatty alcohol reactant to obtain a boron-containing esterification product; 2, carrying out oscillation extraction separation on the boron-containing esterification product by using an organic solvent to obtain a loaded organic phase and a raffinate water phase; 3, the loaded organic phase reacts with a stripping agent, oscillation extraction separation is conducted, and a regenerated raffinate organic phase and an extraction water phase are obtained; and 4, measuring the content of the 10B in the raffinate water phase and the extraction water phase, and determining the enrichment direction of the 10B. According to the method, the separation coefficient of the boron isotope is improved through esterification and hydrolysis, the solvent consumption is reduced by adopting a solvent recycling method, and the economical efficiency of the process is improved.

The invention discloses an esterification reaction device for preparation and protection of malonic acid mono-p-nitrobenzyl ester, and belongs to the field of chemical equipment, the esterification reaction device comprises a fixed cylinder and a reaction kettle, the lower end of the inner surface of the fixed cylinder is fixedly connected with a mounting seat, the outer surface of the upper end of the mounting seat is provided with a sliding groove, and the inner side of the sliding groove is provided with a ball; the upper sides of the outer surfaces of the balls are in rolling contact with the lower end of the reaction kettle, and the outer surface of the reaction kettle is fixedly connected with a heating plate. The stirring assembly is arranged to stir the nitrobenzyl alcohol and the Meldrum's acid in the reaction kettle, so that the mixing uniformity of the organic solvent, the nitrobenzyl alcohol and the Meldrum's acid can be effectively improved, and the preparation and processing quality of the malonic acid mono-p-nitrobenzyl ester can be further improved; and meanwhile, water generated in the esterification reaction can be discharged in a water vapor form, so that the yield of the malonic acid mono-p-nitrobenzyl ester can be improved, and the difficulty of subsequent separation and purification of the malonic acid mono-p-nitrobenzyl ester can be reduced.