Method for continuous enzymatic synthesis of n-3PUFA glyceride

Abstract

The invention relates to a method for continuous enzymatic synthesis of n-3PUFA glyceride, comprising the following steps: preparing fish oil n-3PUFA (EPA, DHA) and glycerol into reaction liquid according to a certain proportion; then pumping reaction liquid into an enzyme reaction column provided with immobilized lipase through a constant current pump for carrying out reaction for enzymatic synthesis of n-3PUFA; finally realizing continuous enzymatic synthetic production of n-3PUFA by controlling circulation flow velocity of the reaction liquid in the reactor and setting a molecular sieve dehydrater in the reaction system. The n-3PUFA glyceride product prepared by the continuous syhthesis method features an ester yield of 30-50%, a monoester content of 20-30%, a diester content of 50-70%and a trig content of 10-20%. With the method of the invention adopted, recycle of enzyme can be realized, cost can be greatly reduced, production cycle can be shortened, production efficiency can beimproved and the operation is easy, so that the method is of great practical value to industrialized batch production.

Description

(1) Technical field [0001] The invention relates to a method for continuously enzymatically synthesizing n-3PUFA glycerides. (2) Background technology [0002] Fish oil n-3 polyunsaturated fatty acid (n-3PUFA)——EPA and DHA have important medical and functional health effects on the human body. As its natural form, n-3PUFA glyceride has stable properties, is not easy to oxidize, and has a good taste. It is most suitable for human digestion and absorption, and has become the best product type for EPA and DHA fish oil health care products and medicines. [0003] Because the content of EPA and DHA glyceride in natural fish oil is relatively low (20~30%), its medical treatment and health care effect are not ideal. In order to increase the content of EPA and DHA glycerides, people have successively used physical methods, chemical methods and enzymatic methods to enrich EPA and DHA glycerides. At present, the enrichment method of using lipase to synthesize DHA and EPA glycerides i...

AI Technical Summary

Problems solved by technology

[0004] However, whether it is physical enrichment method, chemical synthesis method or enzyme-catalyzed synthesis method, it belongs to the batch production mode. The batch reaction is only suitable for the reaction process with small batch and slow reaction rate, and cannot realize the continuous synthesis reaction. It is not suitable for industrial scale production

Especially in the process of enzyme-catalyzed reaction, due to the accumulation of products, the enzyme-catalyzed reaction will be inhibited to a certain extent, thus affecting the balance of the reaction and hindering the forward progress of the enzyme-catalyzed reaction.

In addition, for the batch reaction, the reaction period is long (24-48h), the production efficiency is low, the recycling operation of the enzyme is cumbersome, and the production cost is high, which limits its application in industrialization