131 results about "Carnosine" patented technology

The invention relates to the technical field of cosmetics, and particularly discloses emulsion type foaming cleaning scrub cream with a skin care effect and a preparation method of the emulsion type foaming cleaning scrub cream. The scrub cream is prepared from the following raw material components in percentage by mass: 8 to 15 percent of glycerol, 0.2 to 0.9 percent of sodium hydroxide, 0.3 to 0.8 percent of citric acid, 0.03 to 0.2 percent of EDTA (Ethylene Diamine Tetraacetic Acid) disodium, 0.5 to 1.5 percent of an auxiliary agent, 1.5 to 3.0 percent of a thickening agent A, 1.5 to 3.0 percent of a thickening agent B, 5.86 to 1457 percent of an emollient, 12 to 22 percent of a surface active foaming agent, 0.8 to 3.0 percent of an emulsifying agent, 2.5 to 13 percent of a cutin stripping agent, 0.7 to 1.3 percent of a preservative, 0.32 to 0.92 percent of a skin conditioning agent and the balance of water, the skin conditioner is a mixture prepared from sodium hyaluronate, tocopherol, papain, nicotinamide, carnosine, panthenol and phytosterol oleate.

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention relates to the technical field of microorganisms, and particularly discloses a PhPepD mutant and application of the PhPepD mutant in fermentation production of L-carnosine. According to the invention, a new PhPepD mutant is screened by means of construction of a mutation library from PepD of different sources and error-prone PCR, site-directed saturation mutation and the like, the amino acid sequence of the PhPepD mutant is shown as SEQ ID NO: 18, and compared with wild type PhPepD protein, the PhPepD mutant has N158D / I176F / E393Q mutation. By using the mutant, a microorganism with increased L-carnosine yield can be obtained, and in recombinant microorganisms capable of producing carnosine without adding beta-alanine, the mutant also shows an effect of increasing the L-carnosine yield, and a new effective method is provided for industrial large-scale production of L-carnosine.

The invention relates to the technical field of food processing safety and environmental pollution control, in particular to a natural compound preparation with a synergistic formaldehyde removal function and a preparation method and application thereof. The natural compound preparation is prepared from the following raw materials in percentage by mass: 5%-10% of carnosine, 0.5%-2% of epigallocatechin gallate, 0.05%-0.2% of edible essence, 10%-20% of a surfactant and the balance of a solvent, and the total percentage is 100%. The invention aims to reduce the exposure risk of specific groups such as chefs and guarantee the health level of the specific groups, the main component carnosine is dipeptide which can be used as a food additive and has good antioxidant activity, and amino in the structure of the carnosine can be subjected to Michael addition reaction with carbonyl of aldehydes. The epigallocatechin gallate is a common polyphenol substance, and phenolic hydroxyl groups in the epigallocatechin gallate can be reacted with aldehydes to capture and remove the aldehydes.

The invention discloses a firming and anti-aging composition containing a Campania acuta bark extract and application of the firming and anti-aging composition, and belongs to the technical field of cosmetics. The firming and anti-aging composition containing the Campanumoea maculata bark extract comprises the following components: the Campanumoea maculata bark extract, a Flos Magnoliae Officinalis extract, a Tremella aurantialba extract and a composite polypeptide, and the composite polypeptide is composed of carnosine, acetyl tetrapeptide-5 and tripeptide-10 citrulline in a mass ratio of 1: (0.4-0.6): (0.2-0.6). Through the synergistic effect of the four components, the firming and anti-aging composition has a good effect of inhibiting the activity of elastase and collagenase.

The invention relates to the technical field of cosmetics, in particular to a skin repairing composition for whitening and resisting light damage and a preparation method. The composition comprises polypeptides such as acetyl tetrapeptide-11, glutathione, decarboxylated carnosine, tripeptide-1 and the like, the effects of whitening and brightening the skin, resisting light aging and repairing the skin are achieved through multi-way, multi-azimuth and multi-target combined action and mutual cooperation, and the effects of improving the skin quality and repairing the skin can be better achieved. The composition disclosed by the invention can be widely applied to various cosmetics.

The invention provides a method for extracting goose carnosine and / or carnosine. The method comprises the steps of low-salt water extraction and high-salt water extraction under alkaline and heating conditions. The extraction method of the goose carnosine and / or carnosine provided by the invention is simple to operate, low in cost and easy for large-scale industrial use.

The invention provides a method for producing L-carnosine, a genetic engineering strain as well as a preparation method and application of the genetic engineering strain, and belongs to the field of genetic engineering. According to the genetic engineering strain provided by the invention, by knocking out a glucose-6-phosphate isomerase coding gene pgi, knocking out an L-threonine / L-homoserine transporter coding gene rhtA or knocking out a dipeptide transfer protein coding gene dppABCD, the yield of extracellular L-carnosine is increased, the shake flask yield of the finally constructed strain reaches 9.1 g / L, the 5L fermentation tank yield reaches 65 g / L, and the yield of the extracellular L-carnosine reaches 9.1 g / L; and the method has a good application prospect in L-carnosine biosynthesis.

The invention discloses snowflake cream with pearly luster and a preparation process of the snowflake cream. The snowflake cream comprises the following raw material components: a phase A: water; butanediol; glycerol; methyl glucitol polyether-20; a polyacrylate cross-linked polymer-6; sodium hyaluronate; potassium hydroxide (90%); 4-hydroxyacetophenone; xanthan gum; phase B: caprylic / capric triglyceride; cetostearyl alcohol; stearic acid; a polydimethylsiloxane; squalane; simmondsia chinensis seed oil; mineral oil; and polyethylene glycol (PEG)-100 stearate. Glyceryl stearate; tocopheryl acetate; isononyl isononanoate; a phase C: 1, 2-hexanediol; carnosine; a centella asiatica extract; collagen; the snowflake cream has the advantages of uniform pearly luster texture and the advantages of traditional snowflake cream.

The present disclosure provides, inter alia, compositions and methods for intranasal delivery of carnosine.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with skin anti-aging, anti-inflammatory and soothing functions and application of the compound active peptide. The compound active peptide is prepared from acetyl hexapeptide-1, palmitoyl tripeptide-5, acetyl dipeptide-1 cetyl ester, copper peptide and decarboxylated carnosine hydrochloride. The compound peptide acts on dermal fibroblasts, so that expression of p16 and p21 genes is reduced, and cell cycle arrest is improved; the compound acts on epidermal keratinocytes and dermal fibroblasts and inhibits COX-2 gene expression; the compound peptide acts on epidermal keratinocytes and dermal fibroblasts, and the ROS level is reduced; the compound peptide acts on PC12 cells, inhibits secretion of acetylcholine, blocks transmission of neural signals, and plays a role in immediate wrinkle removal.

This invention belongs to the field of cosmetic technology and discloses a composition containing polypeptides, its preparation method, and its application. The composition provided by this invention comprises: polypeptides and a solubilizing and penetration-enhancing complex; the polypeptides include at least one selected from Codonopsis pilosula cyclic peptide B, conopeptide, carnosine, acetyl tetrapeptide-5, acetyl hexapeptide-8, and nonapeptide-1; the solubilizing and penetration-enhancing complex comprises the fermentation product filtrate of Kluyveromyces lactis / Ganoderma lucidum fruiting body extract, polyols, polysorbate, and ferulic acid. The composition provided by this invention, through the synergistic effect between its components, can effectively improve the solubility and stability of the polypeptides, preventing precipitation or exudation, while effectively promoting the transdermal absorption of the polypeptides; the composition provided by this invention has excellent antioxidant and anti-aging effects.

This invention relates to an anti-wrinkle repair composition containing red camellia extract and its application. The anti-wrinkle repair composition of this invention comprises the following components in weight percentages: polyol 1: 1-25%; red camellia extract: 0.1-15%; creatine: 0.1-2%; carnosine: 0.01-0.5%; recombinant collagen: 0.01-5%; tetrahydrocurcumin inclusions: 1-20%; ergothioneine: 0.0001-1%; water: balance. This invention combats aging through multiple pathways and approaches, providing multi-dimensional skin anti-oxidation and anti-glycation, scavenging free radicals, promoting the production of type I collagen in the skin's extracellular matrix, and strengthening the skin barrier. This composition can be used as an active ingredient in various types of cosmetics, including aqueous solutions, lotions, creams, and shampoos.

The invention discloses a skin care moisturizer preparation method, which comprises: S1, heating an oil phase component to a molten state under a vacuum condition to obtain a homogeneous oil phase; s2, mixing and swelling a water phase component containing a macromolecular thickening agent under a heating condition to obtain a homogeneous water phase; s3, mixing the water phase and the oil phase under a vacuum condition, shearing and homogenizing at 70-80 DEG C to form a primary emulsion, and then starting to cool; s4, when the temperature of the system is reduced to a first preset temperature interval, adding a first group of active substances and uniformly dispersing; and S5, when the temperature of the system is continuously reduced to a second preset temperature interval, adding a second group of active substances, uniformly dispersing, and then adjusting the pH value of the system to 5.5-6.0. According to the present invention, the ceramide, the recombinant collagen and the decarboxylated carnosine are adopted, and the ceramide and the squalane are adopted to simulate the skin lipid so as to actively repair the barrier;

The present disclosure provides, inter alia, compositions and methods for intranasal delivery of carnosine.

The invention discloses a construction method and application of chassis cells for synthesizing carnosine and derivatives thereof, and belongs to the technical field of bioengineering. Compared with the original host cell, the mutant chassis cell formed by the construction method of the multiple escherichia coli gene mutant strains provided by the invention not only effectively relieves the degradation of carnosine, but also keeps the growth performance consistent with that of the original strain, and ensures that the chassis cell normally grows by utilizing a general culture medium and culture conditions. Compared with existing other chassis cells, culture medium components and culture conditions do not need to be changed; compared with an original host cell, the chassis cell disclosed by the invention has the advantages that the degradation of carnosine is obviously relieved; the chassis cell provided by the invention is used for respectively recombining three recombinases from different sources, and compared with the original recombinant cell, the L-histidine conversion rate of a cell-free extraction liquid catalytic system is maximally increased by 20.6%, and the L-histidine conversion rate of a whole-cell catalytic system is increased by 10.5%.

The invention discloses a preparation method of a piezoelectric MOF (Metal Organic Framework) sound-sensitive agent with an ultrasonic response ROS / CO synergistic antibacterial function. The preparation method comprises the following steps: synthesizing a catechol modified ruthenium carbonyl carbon monoxide donor through a coupling reaction of 3, 4-dihydroxyl-1-phenylalanine and carbonyl ruthenium chloride; the method comprises the following steps: mixing an L-carnosine aqueous solution and a zinc ion aqueous solution in a solvent, and self-assembling the two solutions by a hydrothermal method to obtain a piezoelectric metal organic framework (PMOF); and modifying the carbon monoxide donor on the surface of the PMOF through a complexation reaction between zinc ions on the surface of the PMOF and phenolic hydroxyl groups of the carbon monoxide donor to obtain the piezoelectric MOF sound-sensitive agent with ultrasonic response ROS / CO synergistic antibiosis. The invention also discloses the piezoelectric MOF sonosensitizer with ultrasonic response ROS / CO synergistic antibacterial effect obtained by the preparation method and application of the piezoelectric MOF sonosensitizer in preparation of sonosensitizer hydrophobic drugs and / or antibacterial agents. The sonosensitizer provided by the invention has excellent biocompatibility, can controllably start release of active oxygen and carbon monoxide through ultrasound to achieve synergistic antibiosis, enhances the antibacterial ability, and has excellent biological membrane penetrating ability.

The invention discloses a composite multi-oligopeptide composition for skin repair and an application of the composite multi-oligopeptide composition. The multi-oligopeptide composition comprises the following components in parts by weight: 1 to 5 parts of acetyl hexapeptide-8, 0.1 to 1.5 parts of acetyl tetrapeptide-3, 1 to 20 parts of acetyl tetrapeptide-9, 0.1 to 1.5 parts of acetyl tetrapeptide-11, 10 to 190 parts of carnosine, 10 to 100 parts of decarboxylated carnosine hydrochloride, 10 to 120 parts of tripeptide-1, 1 to 50 parts of tetrapeptide-4, 1 to 20 parts of hexapeptide-9, 1 to 10 parts of palmitoyl pentapeptide-4, 1 to 5 parts of palmitoyl tetrapeptide-7, 1 to 10 parts of palmitoyl tripeptide-1, 1 to 35 parts of palmitoyl tripeptide-8, 0.1 to 2.5 parts of oligopeptide-1, 0.1 to 0.5 part of oligopeptide-2 and 0.1 part of carnosine. And 1 part of oligopeptide-5. The multi-oligopeptide composition has excellent anti-wrinkle and anti-aging effects, has the effects of promoting tissue repair and wound healing, strengthening skin barrier and resisting inflammation and relieving, is applied to skin medicine and skin beauty, and has the effects of promoting regeneration and remodeling of an extracellular matrix (ECM), regulating inflammation, blocking inflammatory aging and strengthening a dermis-epidermis joint (DEJ).

The invention belongs to the technical field of biological medicines, and particularly relates to a pharmaceutical composition, which comprises: (a) carnosine; and (b) at least one antibiotic selected from the group consisting of aminoglycoside antibiotics and polymyxin antibiotics. Researches find that carnosine has the effect of enhancing the bactericidal effect of aminoglycoside antibiotics or polymyxin antibiotics, carnosine and the two antibiotics are jointly used for resisting bacterial infection, and the carnosine and the two antibiotics have a remarkable synergistic effect and can jointly act to achieve the remarkable bacterial inhibition effect which cannot be achieved by a single antibiotic.

This invention discloses a multi-target anti-aging composition and its application in cosmetics. By weight, it comprises the following ingredients: 0.001–3 parts hydroxypinazone retinate, 0.01–5 parts hydroxypropyl tetrahydropyranotriol, 0.01–0.5 parts recombinant human collagen, 0.5–3 parts tocopheryl acetate, 0.1–5 parts glyceryl glucoside, 0.01–5 parts Babao Essence Extract, 0.01–0.5 parts carnosine, 0.5–2 parts 3000 peptides, 0.1–5 parts Bifida ferment lysate, 0.01–0.5 parts coenzyme Q10, and 0.1–5 parts black tea fermentation product. This multi-target anti-aging composition targets four aspects: anti-aging, anti-oxidation, anti-glycation, and anti-photochemical activity. The four categories of components work synergistically to significantly enhance the anti-aging efficacy of the product. Human efficacy evaluation tests show that after using the product of this invention for 28 days, the elasticity of the skin around the eyes is significantly increased, the firmness and roughness of the skin around the eyes are significantly improved, and the area, volume and depth of wrinkles at the corners of the eyes are significantly improved.

The invention relates to the technical field of pet food, in particular to an eye-benefiting and liver-protecting fermentation type canine nutrient and a preparation method thereof, and the eye-benefiting and liver-protecting fermentation type canine nutrient comprises the following components: a liver-protecting component, an eye-benefiting component, a chitosan-calcium compound solution, beer yeast powder, honey, lactobacillus plantarum freeze-dried powder and bacillus subtilis freeze-dried powder. Porous starch of the liver protection component carries and protects N-acetylcysteine, and carnosine and quercetin modified by zein remove fermentation byproducts. The eye benefiting component is loaded and protected by octenyl succinic acid modified starch, and the dispersity and the stability of the xanthophyll are improved by grafting a hydrophobic cavity of sulfobutyl-beta-cyclodextrin. The laccase fixed on the surface of the porous starch catalytically oxidizes phenolic hydroxyl groups in a tannic acid-gelatin network on the surface of the eye benefiting component by using dissolved oxygen, a protective shell is formed by in-situ crosslinking and curing, and a chitosan-calcium compound solution closes the physical distance between the laccase and the tannic acid-gelatin network, so that the enzymatic oxidation reaction rate is increased.

The invention belongs to the technical field of skin care products, and relates to an L-carnosine-sophorolipid micelle and a preparation method and application thereof.The method comprises the steps that sophorolipid is dissolved in methyl alcohol or ethyl alcohol or acetone, L-carnosine is dissolved in an aqueous solution with the pH ranging from 2 to 7, after mixing and stirring are conducted according to the mass ratio of 3: 1-8: 1, vacuum rotary evaporation is conducted under the pressure ranging from-0.06 MPa to-0.08 MPa at the temperature ranging from 30 DEG C to 70 DEG C, an organic solvent is removed, a thin film is formed, and the L-carnosine-sophorolipid micelle is obtained. And hydrating with normal saline, purifying with a 1000Da ultrafiltration membrane, and freeze-drying to obtain the product. The obtained micelles are uniform in particle size, the encapsulation efficiency is up to 99.34%, the carnosinase resistance and the transdermal absorption efficiency of the L-carnosine can be improved, and meanwhile, the effects of scavenging free radicals, resisting inflammation, inhibiting bacteria and preserving moisture for a long time are synergistically reserved. The process is simple, convenient and controllable, the problems that the L-carnosine is poor in stability and low in bioavailability are solved, and the preparation method is suitable for smearing type skin care products and has important application value.

The application provides a method for improving the effect of sperm freezing preservation, and particularly relates to adding sperm into a freezing preservation solution containing carnosine to perform freezing preservation. The freezing preservation solution added with carnosine can effectively improve the activity of the sperm after freezing. The addition of carnosine into the sperm freezing preservation solution can effectively improve the quality of the sperm after freezing preservation, and provides certain technical support for sperm preservation.

The invention provides a transferase and a green enzymatic carnosine production method. The high-activity mutants of DmpA-2 and DmpA-6 are obtained by performing single-point or double-point mutation on the 86th site (E86V) and the 259th site (D259A) of wild type DmpA enzyme, so that the catalytic efficiency of the beta-amino acid ester acyltransferase is remarkably improved. According to the method, beta-alanine and L-histidine serve as substrates, recombinant bacteria are used for expressing mutant enzyme, carnosine is catalytically synthesized under the mild condition, the product concentration reaches 5.36 g / L and is 3.53 times that of a wild type, and the method has the advantages of being green in reaction condition, high in product purity and the like and is suitable for industrial production.

The invention discloses a polypeptide composition with functions of whitening and brightening skin, resisting inflammation, resisting light aging and repairing skin, and relates to the technical field of skin care products. The polypeptide composition is prepared from oligopeptide-1, dipeptide-15, hexapeptide-9, palmitoyl tripeptide-5, tripeptide-2, palmitoyl tetrapeptide-7 and carnosine. According to the composition, through the synergistic effect of multiple active peptides, the effects of whitening and brightening skin, resisting inflammation, resisting light aging and repairing skin are achieved through multiple ways and multiple aspects, the effects of improving wrinkles, repairing skin, brightening skin color and delaying senescence can be better achieved, and the composition can be widely applied to various types of cosmetics.

The invention relates to the technical field of enzyme engineering and bioactive peptide preparation, and discloses a preparation method of horse myocardial peptide with antioxidant and antithrombotic effects, and the preparation method comprises the following steps: S1, pretreating horse heart tissue to obtain horse myocardial pulp; s2, composite enzymatic hydrolysis and guiding agent regulation and control: adding a composite enzyme system and an anti-thrombus guiding agent into the horse myocardial plasma; and S3, affinity chromatography enrichment. According to the method disclosed by the invention, a composite enzymolysis, guide regulation and control and affinity enrichment synergistic process is constructed, so that the technical problems of incomplete tissue crushing, low enzymolysis efficiency, insufficient bioactive peptide yield and easiness in oxidation and inactivation caused by high proportion of IIa type muscle fibers and compact connective tissues of horse myocardium are solved in a targeted manner, and a compound enzyme system with a specific ratio is adopted to adapt to the characteristics of the horse myocardium; the enzymolysis efficiency is greatly improved, a cationic guiding agent is introduced to directionally improve the generation proportion of antithrombotic peptide fragments, efficient enrichment of target peptides is achieved by combining thrombin affinity chromatography, and the antithrombotic function is remarkably enhanced while high antioxidant activity is guaranteed.

The application discloses a kind of polypeptide and collagen-containing composition and its application, the composition includes polypeptide and collagen;The polypeptide includes palmitoyl pentapeptide-4, decarboxyl carnosine, acetyl octapeptide-3 and hexapeptide-11;The collagen is recombinant type XVII collagen;The mass ratio of polypeptide and collagen is 12:10 or 12:20.The application adds recombinant type XVII collagen in polypeptide composition, can significantly improve the permeability of polypeptide composition on the one hand, and can improve the generation of collagen, the compounding of polypeptide composition and recombinant type XVII collagen can achieve synergistic effect, improve COL7A1 and COL17A1 gene relative expression, to enhance the overall anti-aging effect.

Provided herein is, inter alia, a hydrogel composition and methods for the treatment of skin conditions such as eczema, dandruff, and acne. The compositions comprise a synergistic blend of active ingredients including salts of carnosine, salts of carnitine, and cyclodextrin, which are formulated to be maintained in solution. The compositions disclosed herein are capable of altering gene expression related to skin health and can be manufactured through a method that involves forming carnosine and / or carnitine salts and combining them with cyclodextrin in solution.

The invention discloses a pentapeptide VE5 with xanthine oxidase inhibitory activity and a preparation method and application thereof, and belongs to the technical field of small molecule peptides. The amino acid sequence of the pentapeptide VE5 is VSIVE, and the pentapeptide VE5 can be prepared through a solid-phase synthesis method and an enzymolysis method. The pentapeptide VE5 is identified from porphyra haitanensis proteolysis liquid and has potential interaction with xanthine oxidase, under the concentration of 0.1 mg / mL, the xanthine oxidase inhibition rate is 67.88%, and compared with goose carnosine (the xanthine oxidase inhibition rate is 36.03%), the xanthine oxidase inhibition rate is remarkably increased (plt; and the compound has higher xanthine oxidase inhibitory activity, and can be used for preparing a preparation for inhibiting xanthine oxidase activity and relieving hyperuricemia.

The invention relates to a collagen mask with skin repairing, anti-aging and brightening effects and a preparation method thereof, and belongs to the technical field of cosmetics. The collagen mask disclosed by the invention is prepared from the following raw materials: deionized water, hyaluronic acid, collagen, natural polysaccharide, a plant composite extract, carnosine, caffeine emulsion, k-carrageenan, nano cellulose and an ionic cross-linking agent. The hyaluronic acid has a moisturizing effect and can open a skin channel, so that caffeine can be absorbed by skin to promote blood circulation, meanwhile, the plant composite extract, carnosine and natural polysaccharide are matched to achieve the effects of repairing, resisting aging and brightening the skin, in addition, k-carrageenan and nano cellulose are added into the mask, and the mask has a good moisturizing effect. The two are combined with metal ions to form a dual cross-linked network structure, so that the elasticity of the mask is improved, and the mask is not easy to break.