A disclosed preparation method for an organosilicon-modified crosslinking polyurethane emulsion is characterized in that: employing diisocyanate, an oligomer polyol, a hydrophilic chain extender and a chain extender to prepare a polyurethane prepolymer, then using a silane coupling agent for end sealing, then adding hydroxyl silicone oil, after reaction, performing cooling, emulsification and pressure-reducing desolvation to obtain the organosilicon-modified crosslinking polyurethane emulsion. According to the preparation method, the silane coupling agent not only helps to combine organosilicon and polyurethane, but also helps to substantially improve crosslinking degree of the material as a cross-linking agent. The emulsion prepared by the invention is good in stability, green and environment-friendly; and the prepared polyurethane film has the advantages of high mechanical strength, low-temperature resistance, good water proofness and the like, and is widely applicable to the fields of paints, fabric coating glues, leather finishing agents, adhesives and the like.