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Method for preparing multifunctional nano-carrier

A nano-carrier and multi-functional technology, applied in the direction of non-active components of polymer compounds, etc., can solve the problem of lack of multi-functional stability of protein nanoparticles, and achieve the effect of good targeting of cancer cells, wide sources, and good controllability

Inactive Publication Date: 2012-07-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, protein nanoparticles prepared by this method lack multifunctionality and good stability, and their surface needs to be modified to achieve multifunctionality.

Method used

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  • Method for preparing multifunctional nano-carrier
  • Method for preparing multifunctional nano-carrier
  • Method for preparing multifunctional nano-carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Adjust bovine serum albumin (BSA) aqueous solution with a concentration of 100mg / mL to pH=7; under magnetic stirring, add ethanol at a rate of 0.5mL / min, the volume ratio of aqueous solution to ethanol is 1:4, and continue Stir, then add 0.8% w / v glutaraldehyde, make the mass ratio of cross-linking agent to BSA 1μg: 1mg, react for 6 hours, centrifuge and wash 3 times, and freeze-dry to obtain BSA nanoparticles. figure 1 ;

[0025] 2) Add a certain amount of polyethylene glycol with a relative molecular mass of 2000, which has a carboxyl group at one end and an aldehyde group at one end, into a 10 mg / mL solution of polyallylamine with a relative molecular mass of 15,000, and maintain the balance between the two. The mass ratio is 4.3:1, adjust the pH value of the solution to 8.5, dialyze after 6 hours of reaction, and freeze-dry to obtain polyallylamine-g-polyethylene glycol-COOH;

[0026] 3) Sodium polystyrene sulfonate (PSS), polyallylamine hydrochloride (PAH) and ...

Embodiment 2

[0031] The steps are the same as in Example 1, but in step 2), polyethylene glycol with a relative molecular mass of 2000 having a carboxyl group at one end and an aldehyde group at one end is added to a 10 mg / mL polylysine solution with a relative molecular mass of 15000 , keep the mass ratio of the two at 4.3:1, adjust the pH value of the mixed solution to 8.5, dialyze after 6 hours of reaction, freeze-dry, and synthesize polylysine-g-polyethylene glycol-COOH; in step 3) -5) Use polylysine (PLL) solution instead of 2mg / mL PAH solution, heparin solution instead of PSS solution, polylysine-g-polyethylene glycol-COOH solution instead of polyallylamine -g-Polyethylene glycol-COOH solution, the transmission electron micrograph of gained multifunctional nanoparticles is shown in Figure 5 .

Embodiment 3

[0033] The steps are the same as in Example 1, but in steps 3) and 4), polylysine solution is used instead of PAH solution, and heparin solution is used instead of PSS solution.

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Abstract

The invention discloses a method for preparing multifunctional nano-carriers through a desolvation technology and a laminated assembling modification technology. Under a stirring condition, in water solution of protein, poor solvent ethanol is gradually dropped, so that the protein is gradually separated from the solution and precipitated with nano-particles; the nano-particle structure is washedby water after the nano-particle structure is stabilized by glutaric dialdehyde cross linking agent; a plurality of polymer electrolyte film layers are alternatively deposited on the surface of the nano-particle, and the polymer electrolyte of short-chain polyoxyethylene is deposited and grafted at the outmost layer so as to obtain the nano-particles modified by the polymer electrolyte; and a targeted molecule adapter is fixed at a carboxyl terminal of the polyoxyethylene so as to obtain the multifunctional nano-carriers. According to the invention, the preparation method is simple, the material resource is easily obtained, and the production efficiency is high; and the obtained nano-particles have excellent stability and good application prospect, and target cancer cells.

Description

technical field [0001] The invention relates to a method for preparing multifunctional nanocarriers, in particular to a method for preparing multifunctional nanoparticle by combining desolvation technology and layer-by-layer assembly modification technology. Background technique [0002] Drug nanocarriers include liposomes, micelles, nanoemulsions, polymer nanoparticles, solid lipid nanoparticles, and flexible vesicles. After surface modification, controllable biological properties can be obtained, and then performed simultaneously in therapy or diagnosis Various important functions. Nanotechnology plays an important role in promoting the development of medicine. The actual needs of disease diagnosis, prevention and treatment have raised expectations for nanotechnology to obtain more advanced drug delivery systems and early detection and diagnostic technologies, such as early diagnosis and early warning; metabolites Discovery of biomarkers and its detection technology for t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08J3/24C08J3/12C08L89/00C08K5/07C08G81/00A61K47/42
Inventor 高长有仝维鋆谢黎黎
Owner ZHEJIANG UNIV
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