53results about How to "Does not cause rejection" patented technology

The invention discloses a mesenchymal stem cell, which is used as a target cell for gene treatment, and used for a carrier of expressing, storing and releasing a transgenic drug. An exogenous promoter and a target gene are led to the mesenchymal stem cell by a virus carrier; and the target gene is integrated into a genome of a host cell, so as to stabilize that the endogenously expressed target gene is not lost and used for gene treatment. It is fully verified that the mesenchymal stem cell can be used as a storage cavern for expressing, storing and releasing a target drug, and has the characteristics of being efficient, safe, reliable and the like by an insulin gene treatment experiment.

The invention discloses a culture method for restoring totipotency of a mesenchymal stem cell. The method for restoring totipotency of a mesenchymal stem cell, provided by the invention, comprises the following steps: 1) performing hanging-drop culture on an in-vitro mesenchymal stem cell with reduced totipotency, thus obtaining a hanging-drop cultured cell; and 2) further performing suspension culture on the hanging-drop cultured cell, thus realizing totipotency restoration of the mesenchymal stem cell. The experiment of the invention proves that, according to the method for restoring totipotency of a mesenchymal stem cell (specifically 3D culture), after the mesenchymal stem cell (in-vitro mesenchymal stem cell with reduced totipotency) subcultured multiple times is subjected to 3D culture treatment, the increased volume is gradually restored, the cell viability is enhanced, the cloning capability and differentiation capability are enhanced, and the paracrine action is also improved, thereby activating the aged mesenchymal stem cell and restoring the characteristics of the stem cell.

The invention relates to a cervical spondylotic myelopathy (CSM) experimental animal model and a making method thereof, belonging to the technical field of osteopathic medicine experimental animal models. According to the technical scheme of the invention, a living rabbit, goat, dog, miniature pig or experimental mouse is used as a model body, a compression nail is placed at C3 cervical vertebra body of the rabbit, goat, dog, miniature pig or experimental mouse, and developing biomaterial is filled inside the filling hole of the compression nail. The making method of the CSM experimental animal model comprises the following steps of: making a hole at the C3 cervical vertebra body, and placing the compression nail into the hole to cause spinal cord ventral compression. The CSM experimental animal model provided by the invention has the following benefits that the C3 cervical vertebra is selected as the vertebral body where the compression nail is placed, which completely breaks the domestic and abroad traditional mode of compression at C4 or C5 cervical vertebra, is a new breakthrough in CSM experimental animal model making and achieves an ideal experimental effect; and the surgical operation is simple and safe, and the change of CSM after long-term chronic compression can be studied in various imaging techniques at the same time.

The invention discloses a personalized bone fracture plate and a manufacturing method thereof. The bone fracture plate made from titanium or titanium alloy through 3D printing is in fit contact with the shape of a patient bone, and the surface of the bone fracture plate is provided with a hydroxyapatite film. The manufacturing method comprises the steps that 1, CTA scanning technology or MRI technology is adopted for carrying out three-dimensional radiographing on the paint bone, and three-dimensional data of the patient bone is obtained; 2, computer aided design is used for displaying the patient bone and determining the shape, size and the corresponding connection way of the bone fracture plate; 3, personalized bone fracture plate data is output, and a metal powder selective laser melting 3D printer is used for printing the bone fracture plate, wherein the adopted titanium or titanium alloy is of the particle diameter of 15-45 micrometers and is in a spherical shape; 4, vacuum heat treatment is carried out on the bone fracture plate obtained through 3D printing; 5, the hydroxyapatite film is produced on the surface of the bone fracture plate.

The invention discloses a fabrication method for a fracture fixator, which includes the following steps: scanning the fracture and the corresponding healthy-side part of a patient, and acquiring initial data; adopting three-dimensional software to convert the initial data into a three-dimensional model, so that a skeleton model at the fracture and the corresponding healthy-side part of the patient is obtained; utilizing the three-dimensional software to carry out a fixation analysis on the skeleton model at the fracture and design a fracture fixator model; preparing bioactive artificial bone material; converting the designed fracture fixator model into a rapid prototyping file format, and feeding the prepared bioactive artificial bone material as prototyping material into a rapid prototyping machine to produce the fracture fixator. The invention has the advantage that the rapid prototyping technique, the three-dimensional computer design and the bioactive artificial bone material are combined to fabricate degradable fracture fixators adapted to different parts and different degrees of individualized shape designs.

The invention discloses yak lactoprotein polypeptide nutrition powder applicable to rehabilitation of people after operations and a preparation method of the powder. The powder comprises the following main components: 20-24% of yak lactoprotein polypeptide powder, 6-10% of sodium caseinate, 18-20% of whey protein, 8-10% of defatted milk powder, 1-2% of bovine coloctrum, 0.02-0.03% of arginine, 0.11-0.14% of taurine, 8-12% of glucose, 1.1-1.5% of a silverweed cinquefoil extract, 0.1-1.4% of a lily extract, 1.5-2.5% of apple powder, 0.1-0.3% of vitamin C, 0.01-0.02% of vitamin E, 0.004-0.008% of group-B vitamins, 8-12% of polydextrose, 5-9% of fructo-oligose and 8-10% of maltodextrin. Through the organic combination of nutrients, energy and nutrient substances required by people after the operations can be comprehensively supplemented, and substances which are required by the people after the operations for the rehabilitation as soon as possible are provided.

The invention discloses an artificial skull repair material, and belongs to the technical field of orthopaedic repair materials. The artificial skull repair material comprises the following components in parts by weight: 15 to 29 parts of bone cement, 11 to 35 parts of a 3D (3-dimensional) titanium mesh, 11 to 17 parts of a polylactic acid composite material chitin and 15 to 21 parts of polyetheretherketone. The invention further provides a preparation method for the artificial skull repair material. A skull structure with a required radian can be rapidly prepared by adopting a 3D printing technology. The skull repair material comprising the components in parts by weight is processed to prepare skull prosthesis with any required radian by adopting the 3D printing technology, and is high in biocompatibility and low in cost, the advantages of materials such as the bone cement and the 3D titanium mesh are integrated, the shortcomings of a common skull repair material in terms of mechanical performance, processibility and consistency are effectively overcome, and meanwhile, side effects in clinical applications are obviously reduced.

The invention relates to a biological composite artificial bone and its preparation process. The artificial bone is prepared by poly lactic acid, hydroxyapatite(or calcium phosphate) and OPG protein with weight ratio of 180-250 : 100-160 : 0.6-1. The invention mixes poly lactic acid, hydroxyapatite(or calcium phosphate) and OPG protein only once by emulsion cross blend method without changing their chemical structure. The adopted ethyl acetate and absolute ethyl alcohol have no harm to the human body, and the hydroxyapatite(or calcium phosphate) bone conduction function and the osteoclast genesis and activation of the OPG protein are kept. It can also initiate osteoclast apoptosis. The compound product is a biological compound artificial bone for treating bone defection and osteoporosis in clinical practice.

The invention relates to a rapid hemostatic. Preparation raw materials of the rapid hemostatic comprise microporous starch, calcium ions or zinc ions, chitosan or chitin. In every 100 g of the microporous starch, the addition amount of the chitosan or chitin is 1 to 50 g, the addition amount of the calcium ions is 10 to 500 micromoles, or the addition amount of the zinc ions is 6 to 80 micromoles. When the rapid hemostatic disclosed by the invention is not used, the temperature of skin at a wound cannot be increased obviously, and sharp temperature rise of the wound during hemostasis can be effectively prevented, so that pains of a patient due to the fact that the temperature of the wound is increased when an existing hemostatic is used are avoided, and the novel hemostatic can be used for hemostasis of internal and external wounds under various middle and small arteries.

The invention belongs to the field of gene engineering, particularly relates to a protein sequence capable of efficiently inhibiting malignant tumors, and further relates to a gene sequence for encoding the protein sequence and application of the protein sequence. The application of the protein sequence as shown in SEQ ID NO.1 in preparation of a preparation for inhibiting the malignant tumors isthe key protected content of the invention. The protein sequence as shown in SEQ ID NO.1 is expressed by a gene sequence as shown in SEQ ID NO.2; and in addition, the gene sequence as shown in SEQ IDNO.2 is also the protected content of the invention. The protein sequence provided by the invention has the following advantages that the protein sequence has an excellent inhibition function in various tumor cells of bladder cancer, lung cancer and the like; the cancer inhibition effect is quick and obvious; and besides, the sequence provided by the invention is a human-derived sequence, does notcause rejection reaction, and has a wide application prospect.

The invention discloses a neuropeptide and a synthesis method and application thereof, wherein the neuropeptide has an amino acid sequence shown by SEQIDNO:1. According to the amino acid sequence represented by the SEQIDNO:1, valine is used as a first amino acid,-NH2 of the valine is exposed, then the-NH2 of the valine is condensed with-COOH of second amino acid-arginine to form an amide bond, other amino acids are sequentially connected, and finally tyrosine is to obtain the neuropeptide. Compared with the prior art, the neuropeptide has the following advantages: 1. the neuropeptide product can effectively improve defects of experimental animal social memory, and no adverse reaction is observed; 2. functional areas of the neuropeptide is a sequence on an endogenous protein, and cell rejection reaction may not be caused; 3. a signaling pathway activated by the neuropeptide is a known nervous system specific signaling pathway; 4. the product can effectively change the activity and state of neurons; and 5. the essence of the product is an amino acid sequence, is easy to degrade, and may not cause bodily organ loads.

The invention discloses a 3D printed artificial periosteum, an artificial bone, an artificial skeleton and a preparation method thereof. The 3D printed artificial periosteum is prepared by compoundingcalcium alginate and nanoscale pig cross-linked collagen fibers as materials through 3D printing according to the shape of the periosteum required by a bone defect part of a patient, wherein the massratio of the calcium alginate to the nanoscale pig cross-linked collagen fibers is 1:(1-5). The 3D printed artificial bone is formed by printing an artificial bone material by using a 3D printer according to the shape of the bone defect part of the patient; and the artificial bone material is prepared from the following components: 1wt%-6wt% of nanoscale pig cross-linked collagen fibers, 3wt%-4wt% of nanoscale hydroxyapatite and 90wt%-96wt% of a degradable biological carrier material. The 3D printed artificial skeleton is composed of the 3D printed artificial bone body and the 3D printed artificial periosteum.

The invention discloses a tissue engineered esophagus and a preparation method thereof. The tissue engineered esophagus contains a dual-layer structure of an epithelial layer and a dermis layer constructed by a scaffold material and seed cells, wherein the scaffold material is acellular matrix, and the seed cells include fibroblasts, vascular endothelial cells, and keratinocytes. The vascular endothelial cells in the dermis layer directly take part in the creation of capillary network, and two revascularization methods are used for quickly communicating with the blood supply channel of receptor, thus the epithele of the tissue engineered esophagus is provided with blood.

The invention relates to a blood glucose regulating polymer vesicle which can be applied to treating diabetes as well as a preparation method and application thereof. The polymer vesicle is prepared from amphipathic glycopolymer. The preparation method of the blood glucose regulating polymer vesicle comprises the following steps: firstly, preparing the amphipathic glycopolymer at least from an initiator, a chain transfer agent and a polymer monomer; then enable the amphipathic glycopolymer to conduct self-assembling to form the blood glucose regulating polymer vesicle. The blood glucose regulating polymer vesicle disclosed by the invention can respond to concentration change of glucose in the external environment to generate particle diameter change, cannot be affected by temperature, further has better stability and can be used as a blood glucose detection agent or a non-drug buffer agent for treating the diabetes.