111results about How to "No inflammatory response" patented technology

The invention provides marine biomaterial composite hydrogel dressing and a preparation method thereof and relates to the technical field of medical dressing. The hydrogel dressing is prepared from asodium alginate-fish collagen mixed solution and a chitosan-crosslinking agent mixed solution, wherein the sodium alginate-fish collagen mixed solution is prepared from sodium alginate, fish collagen,polyvinyl alcohol, sodium carboxymethyl cellulose, pectin, glycerin, propanediol and distilled water; the chitosan-crosslinking agent mixed solution is prepared from chitosan, a crosslinking agent, glacial acetic acid and distilled water. The invention also provides the preparation method of the dressing. The sodium alginate-fish collagen mixed solution is cultured at the constant temperature, the chitosan-crosslinking agent mixed solution is added, the mixture reacts, and the hydrogel dressing is obtained. The hydrogel dressing has excellent mechanical property, moisture retention and biocompatibility, accelerates wound repairing, can diminish inflammation and perform sterilization, has no wound inflammation reaction, effectively promotes wound healing, is easy to remove and does not stick to a wound surface.

The invention relates to the technical field of bone tissue engineering, and discloses a step-by-step tissue engineering bone building method. The method comprises the following steps of: (1) stent repair and pre-vascularization: building a tissue engineering bone sent at a bone defect part, and performing pre-vascularization; (2) later embedding of bone tissue engineering seed cells: after inflammatory reaction stage, embedding the seed cells, subjected to osteogenic induction, into the tissue engineering bone sent at the bone defect part based on growth-in time of capillaries, wherein the later embedding of bone tissue engineering seed cells in the step (2) is carried out 7-14 days later after the stent repair and pre-vascularization in the step (1). In the method provided by the invention, the sent promoting growth of vessels is firstly embedded, the seed cells for osteogenesis are embedded later, thus, a great segment of tissue engineering bone can be built quickly, application amount of the bone tissue engineering seed cells needed for repairing per unit volume of bone tissue is reduced greatly, and utilization rate of the seed cells is improved.

The invention discloses a CS (calcium silicate)/PEEK (poly(ether-ether-ketone)) composite bone repair material, a bone repair body as well as a preparation method and an application of the material. In the CS/PEEK composite bone repair material, CS accounts for 30%-50% and PEEK accounts for 50%-70%; the particle size of CS is 20-30 mu m, and the particle size of PEEK is 30-40 mum. The CS/PEEK composite bone repair material has excellent mechanical properties, has the elasticity modulus similar to that of natural bone, has better osteogenesis bioactivity, has excellent mechanical compatibility, cannot cause bone resorption due to stress shielding, can promote regeneration of peripheral bone tissue and fusion with natural bone tissue after being implanted into a human body and can meet requirements of immediate fixation and long-term stability of postoperative orthopedic instruments.

The invention discloses a mesoporous calcium magnesium silicate and polyetheretherketone composite, bone prosthesis as well as a preparation method and application of the composite. The preparation method of the mesoporous calcium magnesium silicate/polyetheretherketone composite comprises the following steps: uniformly mixing 20-40wt% of mesoporous calcium magnesium silicate and 6-80wt% of polyetheretherketone composite of 10-20microns so as to obtain mixed powder; mixing the mixed powder with absolute ethyl alcohol, ultrasonically dispersing, and evaporating the absolute ethyl alcohol at 58-62 DEG C so as to obtain composite powder; and pressing and molding the composite powder by virtue of a mould pressing method so as to obtain the composite. The mesoporous calcium magnesium silicate is uniform in particle size and is uniform in mesoporous pore size distribution. The composite is good in bioactivity and biocompatibility, relatively good in mechanical compatibility with bone tissue, and capable of stimulating bone growth, accelerating bone healing and shortening healing time of bone after implanting material. The bone prosthesis is good in bone mechanical compatibility and antibacterial property, high in strength, fatigue-resistant, good in corrosion resistance and long in service life.

The invention discloses a preparation method of a pelvic floor patch composite biological material. The preparation method comprises the specific step of treating accellular matrix gel and a polypropylene material at pressure of at least 100MPa for at least 5 minutes. The preparation method is simple, enables the accellular matrix gel and the polypropylene material to be combined firmly, does not utilize chemical crosslinking agent in a crosslinking process, and is free from chemical residuals. The invention also discloses the pelvic floor patch composite biological material prepared by the method, and application of the material. Animal experiments prove that the material has the advantages of being good in tissue compatibility and being free from inflammatory response and can be used for treating pelvic organ prolapse.

The invention belongs to the field of biomedical engineering, and relates to a human tissue-engineered cardiac muscle tissue and a preparation method thereof. The human tissue-engineered cardiac muscle tissue disclosed by the invention is prepared by inoculating all kinds of cell components of a normal heart differentiated from human induced multipotential stem cells by taking a natural decellularized heart substrate as the scaffold. The human tissue-engineered cardiac muscle tissue has cell components, extracellular matrixes and biological functions similar to that of a normal human cardiac muscle tissue; testing and screening of in-vitro drugs, initial in-vivo transplantation researches of clinical earlier-stage small animals and observation of treatment effects can be carried out; and helps can be finally offered for realizing individualized treatment of human cardiovascular diseases.

The invention discloses a polypeptide microneedle and a preparation method thereof. The polypeptide microneedle comprises a base and a needle body fixed to the base, wherein the base is made of a high polymer material, and the high polymer material is selected from polyvinyl alcohol, polycarboxymethyl cellulose, chondroitin sulfate, polylactic acid-polyglycolic acid copolymer, fibroin, dextrin, hyaluronic acid or gelatin; and the needle body is prepared from the following raw materials: bioactive beautifying polypeptide, hyaluronic acid and deionized water. The microneedle is simple in preparation method, low in cost and suitable for mass production, can be widely applied to the field of cosmetic polypeptide, and has the excellent effects of removing wrinkles, resisting ageing, removing acnes, repairing the skin, whitening the skin, removing freckles, relieving the skin, resisting allergy, removing under-eye puffiness or promoting hair growth and strengthening hair.

The invention belongs to the technical field of construction of artificial organs by a tissue engineering method in biomedical engineering and relates to a tissue engineered breast transplant and a constructing method thereof. The tissue engineered breast transplant of the invention takes injectable material I type collagen gel as a carrier bracket and takes adipose-derived stem cells as seed cells; the seed cells are attached to the carrier bracket to form an adipose-derived stem cell/I type collagen gel compound; the final concentration of collagen is 2 mg/ml; and the cell density is 2*10<7>/ml. The tissue engineered breast transplant of the invention consists of injectable material-loaded autologous adipose-derived stem cells and is simple and convenient to prepare. Experiments prove that a new adipose tissue can be produced and the tissue engineered breast transplant is finally converted into autologous fat without an immunological rejection problem, so a new solution is provided for the filling and reconstruction of a breast.

A human anti-HBsAg mono-chain antibody/human antibody light chain constant region/protamine truncate recombinant gene, encoding protein and use are disclosed. The protein has antigen and nucleic acid combined activities. The process is carried out by: combining HBV siRNA with siRNA expression plasmid, transferring to HBV infectious cell specifically, and inhibiting HBV gene expression and copy. It has excellent specificity, efficiency, continuity and safety.

The invention relates to a preparation method of a mulberry silk tissue engineering scaffold. Artificially synthesized macromolecular material PLGA (polylactic-co-glycolic acid) is adopted in the traditional method, degradation products of the PLGA are acid, local pH value is reduced and inflammation can be easily caused. The preparation method provided by the invention comprises that mulberry silk is taken as a raw material and is dissolved with CaCl2 solution with the mass fraction of 40% after being refined by Na2CO3 solution with the concentration of 0.5g/dL, then filtering is carried out by adopting a dialysis bag, the obtained fibroin protein solution is added into mixed solution of chitosan/acetic acid, stirring to be uniform is carried out, then mixture is added into a charging barrel of a sprayer of forming equipment, low-temperature forming is carried out, a freezing scaffold is placed into a freeze drying machine, freeze drying is carried out, and a scaffold which is in solid state at normal temperature is obtained. The degradation products of the mulberry silk tissue engineering scaffold prepared by the preparation method provided by the invention are polypeptides, local pH value can not be reduced, and inflammation can not be caused, thus the mulberry silk tissue engineering scaffold is harmless to human body.

The invention relates to a uterine cavity implant as well as a preparation method and application thereof. The uterine cavity implant is characterized by having a bagged structure. The uterine cavity implant comprises a substrate material of animal small intestine submucosa subjected to decellularization treatment. An implantable medical device for preventing and treating intrauterine adhesion comprises a biological tissue substrate material. The present decellularization technology for a biological repairing material is improved; compared with the present product, the biological tissue substrate material provided by the invention has the advantages of lower DNA residual quantity, lower immunogenicity, higher anti-infection capacity and higher repairing capacity; and the uterine cavity implant is beneficial to the recovering of tissues, such as, uterine cavity base layer, submucosa and mucous layer. Besides, the invention prepares the biological repairing material into the uterine cavity built-in structure which is used for isolating wound surfaces, repairing uterine cavity tissues and reducing scar forming, so that the intrauterine adhesion problem is solved, and the accordingly generated sterility problem also can be solved.

The invention discloses a hemostat, comprising gelatin, microbial transglutaminase zymogen, and an activating agent of the microbial transglutaminase zymogen. The ratio of gelatin to microbial transglutaminase zymogen to the activating agent is 100000:1-200:1-100. The invention also discloses a preparation method and an application of the hemostat. The hemostat provided by the invention has better stability and hemostatic effect, and enhanced safety and reliability.

The invention discloses a preparation method of spherical crystal calcined bone. The preparation method comprises the following steps: firstly, taking an allogeneic bone, removing soft tissue, performing coarse cutting, soaking in a NaOH solution and an H2O2 aqueous solution to remove immunogen and part of impurities, then performing primary low-temperature calcination, soaking in a mixed solution of sodium tripolyphosphate and dicalcium phosphate after calcination, and finally performing secondary high-temperature calcination. The spherical crystal calcined bone prepared by the method has higher compressive strength and breaking strength, does not generate inflammatory response when being implanted into a body, and has high biocompatibility. According to the invention, a secondary calcination technology is adopted, and a key step of adding a mixed solution of sodium tripolyphosphate and dicalcium phosphate to soak is adopted in the secondary calcination, so that the prepared material is enhanced in strength, improved in biocompatibility and suitable for bone transplantation.

The invention relates to the technical field of medical preparations and particularly relates to a preparation method of a collagen-tussah silk fibroin composite bracket loaded with BMSCs (bone marrowmesenchymal stem cells). The preparation method of the collagen-tussah silk fibroin composite bracket loaded with BMSCs comprises the following steps: step 1, preparing a collagen solution; step 2, preparing a tussah silk fibroin solution; step 3, preparing a suspension of the BMSCs; and step 4, preparing the composite bracket. The prepared collagen-tussah silk fibroin composite bracket loaded with the BMSCs is good in biocompatibility, free of inflammatory reaction, toxic reaction, immunological rejection reaction and the like, strong in cell adhesion and capable of promoting the repair of askin wound; and furthermore, the composite bracket can keep the humidity of the wound to prevent loss of body fluid and moisture, can provide a biological barrier to prevent wound infection and bacterial invasion, and has certain flexibility and tensile property to keep the functions and form of the composite bracket during tissue healing.

The invention provides medical dressing capable of promoting wound healing. The medical dressing is prepared from the following raw materials in parts by weight: 100 to 200 parts of gelatin, 50 to 100parts of sodium alginate, 80 to 160 parts of polylactic acid and 20 to 150 parts of mannose. According to the medical dressing capable of promoting the wound healing, all the raw materials are high molecular materials with good biological compatibility; the polylactic acid is added, so that the obtained medical dressing has an anti-inflammatory effect and has a good effect of promoting the woundhealing.

The invention provides a device for controlling animal follicle development and preventing and treating reproductive tract inflammation and a preparation method thereof. The device is internally provided with a nylon support, and a sustained-release material layer with estrogen, progestational hormones and antibacterial peptide is wrapped around the outer part of the support. The device is provided with a connecting part and two long arms, the bottom ends of the two long arms are connected to the connecting part, a certain included angle is formed between the two long arms, and the portions, at the long arms, of the sustained-release material layer protrude to form a plurality of sheet structures which are orderly arranged. According to the device and the preparation method thereof, medicines and silicone rubber raw materials are mixed according to a certain ratio, and after low-temperature vulcanization forming is carried out, the medicines combined with silicone rubber and a high polymer material support are prepared into a special vaginal embedding bolt; the medicines combined with the silicone rubber can be slowly released for a long time (10 days or more), so that the effectsof effectively controlling the animal follicle development and preventing and treating the vagina and uterine inflammation can be achieved; the adopted materials are non-toxic, harmless and smooth andsoft in texture and have a good compatibility with animal tissues; and the pick-and-place operation of the device is simple.

The invention discloses an antibacterial fibroin-based bionic coating and a preparation method thereof. The bionic coating is prepared from the following raw materials: fibroin, caffeic acid dopamine, dopamine hydrochloride, chitosan fibers, polyurethane, boric acid, calcium metaphosphate, strontium fluorapatite, a seaweed extract, galuteolin, puffball powder, zinc oxide, strontium gluconate, glycine fumarate, potassium hydroxide, polyvinyl alcohol, distilled water, glycol salicylate and ethanol. The bionic coating provided by the invention is high in bonding intensity with a metal implant, low in coefficient of wear on a steel ball and excellent in wear resistance, and the bionic coating is excellent in antibacterial effect on staphylococcus aureus; and meanwhile, the bionic coating is good in biocompatibility, free from cell toxicity, free from formation of blood coagulation and thrombosis and free from any inflammatory reaction, and the bionic coating has a good clinical application value.

The invention discloses an elastin-like silk fiber porous composite material. The preparation raw materials of the porous composite material comprise elastin-like polypeptide and silkworm cocoons. A preparation method comprises the concrete steps that silkworm cocoons are cut up; degumming is performed to obtain silk fiber; the elastin-like polypeptide and the silk fiber are subjected to crosslinking to prepare the porous composite material. The composite material can be easily processed and formed; after cobalt 60 irradiation sterilization, the material performance is not influenced. The composite material has no influence on peripheral tissues, and can be melted into a whole with the peripheral tissues; meanwhile, the degradation rate of the composite material is matched with the bone or cartilage regeneration speed; the support effect can be continuously and stably provided for the cell growth.

The invention discloses novel application of a natural silk sericin-free silk fibroin core to preparation of a medical surgical instrument for human bodies. The natural silk sericin-free silk fibroin core has high mechanical property, high flexibility and an appropriate degradation speed. As proved by an in-vivo experiment on an animal, the natural silk sericin-free silk fibroin core is immune to exclusive reactions and toxic reaction, and is a good material for preparing various in-vivo medical instruments.