Natural silk sericin-free silk fibroin core and application thereof
A technology of natural silk and silk fibroin, applied in medical science, prosthesis, surgery, etc., can solve the problems of spermatic cord compression, effusion, foreign body sensation, etc., and achieve the effect of wide application range and good mechanical strength
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Embodiment 1
[0021] Weaving natural silk single or double raw silk into a rectangular mesh patch, the size specifications are 6×7.5cm, 6×13.8cm, 15×15cm, 25×35cm, and the mesh diameter is 2-4mm. Take 0.01 g of sodium carbonate and 0.3 g of sodium lauryl sulfate, dissolve them in 100 ml of deionized water, and make a degumming alkali solution; put the mesh patch in the degumming alkali solution, shake and degumming at 80°C for 10 Minutes, then discard the alkali solution and add an equal amount of citric acid with a concentration of 1% by weight to neutralize, then shake and wash with deionized water 5 times, each time for 30 minutes.
[0022] Getting pH is 200ml of 0.1M phosphate buffer solution of 8, adding 1 gram of pronase is mixed with 0.1M containing pronase phosphate buffer solution of 8, the silk patch after cleaning is immersed in this solution, Shake at 60°C for 120 minutes for further enzyme degumming; after the enzyme degumming is completed, heat the solution to 85°C and keep it...
Embodiment 2
[0025] To weave natural silk raw silk into surgical medical equipment, take 1 gram of sodium carbonate and 0.03 gram of sodium lauryl sulfate, dissolve them in 100 ml of deionized water to make a degumming alkali solution; put the medical equipment into the degumming alkali solution, Shake degumming at ℃ for 10 minutes, then discard the alkali solution and add an equal amount of citric acid with a concentration of 1% by weight to neutralize, and then shake and wash with deionized water 5 times, 50 minutes each time.
[0026] Take 300ml of a 0.1M phosphate buffer solution with a pH of 8, add 1 gram of pronase to prepare a 0.1M phosphate buffer solution containing a pronase with a pH of 8, immerse the cleaned medical equipment in the solution, and heat at 80°C Shake for 90 minutes to carry out further enzyme degumming; after the enzyme degumming is completed, the solution is heated to 65° C. for 50 minutes to inactivate the enzyme activity. Take out the medical equipment, wash i...
Embodiment 3
[0028] Take 200 ml of a 0.1M phosphate buffered saline solution with a pH of 8, and add 1 gram of pronase to prepare a 0.1M phosphate buffered saline solution containing a pronase with a pH of 8.
[0029] Weave natural silk raw silk into blood vessels; take 1 gram of sodium carbonate and 3 grams of sodium lauryl sulfate, dissolve them in 100 ml of deionized water to make a degumming alkali solution; put artificial blood vessels in the degumming alkali solution at 70 ° C Shake and degumming under the conditions for 50 minutes, then discard the alkali solution and add an equal amount of citric acid with a concentration of 1% by weight to neutralize, then shake and wash in deionized water for 5 times, each time for 40 minutes; after that, immerse the artificial blood vessel in 0.1M In the phosphate buffer solution containing pronase, shake at 40°C for 50 minutes to carry out enzyme degumming; after the enzyme degumming is completed, heat the solution to 55°C for 10 minutes to inac...
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