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Preparation method of collagen-tussah silk fibroin composite bracket loaded with BMSCs (bone marrow mesenchymal stem cells)

A technology of tussah silk fibroin and composite scaffolds, which is applied in the field of preparation of collagen-tussah silk fibroin composite scaffolds, can solve problems such as poor differentiation ability, and achieve the goal of promoting wound healing, preventing wound infection, and preventing body fluids and moisture churn effect

Inactive Publication Date: 2018-10-09
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the biomedical materials used for wound repair treatment in the prior art have the defect of poor differentiation ability, therefore, there is an urgent need to improve the prior art

Method used

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  • Preparation method of collagen-tussah silk fibroin composite bracket loaded with BMSCs (bone marrow mesenchymal stem cells)
  • Preparation method of collagen-tussah silk fibroin composite bracket loaded with BMSCs (bone marrow mesenchymal stem cells)
  • Preparation method of collagen-tussah silk fibroin composite bracket loaded with BMSCs (bone marrow mesenchymal stem cells)

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A preparation method of BMSCs-loaded collagen-tussah silk fibroin composite scaffold includes the following steps:

[0042] Step 1. Preparation of collagen solution: extract collagen from animal tissues rich in collagen by acid method to obtain a crude extract, and then purify the crude extract to obtain a pure collagen solution; wherein, the purification includes successively Centrifugation, salting out and ultrafiltration treatment;

[0043] Step 2. Preparation of tussah silk fibroin solution: the tussah silk is degummed, and the treated tussah silk is put into the dissolving solution and heated at 55°C to dissolve it to obtain the crude tussah silk fibroin extract solution. The crude protein extract is filtered and dialyzed to obtain a tussah silk fibroin solution; in this example, the degumming process is to put tussah silk in an alkaline solution and boil for 40 minutes, then wash with water, and repeat the degumming twice; this example Wherein, the dissolving solution...

Embodiment 2

[0047] A preparation method of BMSCs-loaded collagen-tussah silk fibroin composite scaffold includes the following steps:

[0048] Step 1. Preparation of collagen solution: extract collagen from animal tissues rich in collagen by alkaline method to obtain a crude extract, and then purify the crude extract to obtain a pure collagen solution; wherein, the purification includes successively Centrifugation, salting out and ultrafiltration treatment;

[0049] Step 2. Preparation of tussah silk fibroin solution: degumming the tussah silk, and then put the treated tussah silk into the dissolving solution and heat it to dissolve at 50°C to obtain a crude tussah silk fibroin extract solution. The crude protein extract is filtered and dialyzed to obtain a tussah silk fibroin solution; in this example, the degumming process is to put tussah silk into an alkaline solution and boil for 30 minutes, then wash with water, and repeat the degumming twice; this example Wherein, the dissolving soluti...

Embodiment 3

[0053] A preparation method of BMSCs-loaded collagen-tussah silk fibroin composite scaffold includes the following steps:

[0054] Step 1. Preparation of collagen solution: extract collagen from animal tissues rich in collagen by salt method to obtain a crude extract, and then purify the crude extract to obtain a pure collagen solution; wherein the purification process includes successively Centrifugation, salting out and ultrafiltration treatment;

[0055] Step 2. Preparation of tussah silk fibroin solution: degumming the tussah silk, and then put the treated tussah silk into the dissolving solution and heat it to dissolve at 60°C to obtain the crude tussah silk fibroin extract solution, and then the tussah silk fibroin The crude protein extract is filtered and dialyzed to obtain a tussah silk fibroin solution; in this embodiment, the degumming process is to boil tussah silk in an alkaline solution for 50 minutes, then wash with water, and repeat the degumming twice; this embodime...

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Abstract

The invention relates to the technical field of medical preparations and particularly relates to a preparation method of a collagen-tussah silk fibroin composite bracket loaded with BMSCs (bone marrowmesenchymal stem cells). The preparation method of the collagen-tussah silk fibroin composite bracket loaded with BMSCs comprises the following steps: step 1, preparing a collagen solution; step 2, preparing a tussah silk fibroin solution; step 3, preparing a suspension of the BMSCs; and step 4, preparing the composite bracket. The prepared collagen-tussah silk fibroin composite bracket loaded with the BMSCs is good in biocompatibility, free of inflammatory reaction, toxic reaction, immunological rejection reaction and the like, strong in cell adhesion and capable of promoting the repair of askin wound; and furthermore, the composite bracket can keep the humidity of the wound to prevent loss of body fluid and moisture, can provide a biological barrier to prevent wound infection and bacterial invasion, and has certain flexibility and tensile property to keep the functions and form of the composite bracket during tissue healing.

Description

Technical field [0001] The invention relates to the technical field of medical preparations, in particular to a preparation method of a collagen-tussah silk fibroin composite scaffold loaded with BMSCs. Background technique [0002] The research progress of stem cells, especially mesenchymal stem cells, provides new ideas and strategies for wound repair. Research in recent years has shown that bone marrow mesenchymal stem cells (BMSCs) can differentiate into epidermal cells, dermal cells, vascular endothelial cells and skin adnexal cells in the skin microenvironment, providing a new source of seed cells for tissue engineered skin. Moreover, basic and clinical studies suggest that mesenchymal stem cells promote skin repair and serve as skin substitutes mainly depending on the active substances they secrete. For example, human amniotic fluid mesenchymal stem cells cultured under hypoxic conditions can promote skin wound healing through TGF-beta / SMAD2 and PI3K / Akt signaling pathway...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/38A61L27/60
CPCA61L27/3834A61L27/48A61L27/60C08L89/00
Inventor 周艳芳彭新生崔碧玲刘雯恩范志强李玉玲
Owner GUANGDONG MEDICAL UNIV
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