212results about How to "Promote wound repair" patented technology

Systems and methods for prophylactic measures aimed at improving wound repair. In some embodiments, laser-mediated preconditioning would enhance surgical wound healing that was correlated with hsp70 expression. Using a pulsed laser (λ=1850 nm, Tp=2 ms, 50 Hz, H=7.64 mJ / cm2) the skin of transgenic mice that contain an hsp70 promoter-driven luciferase were preconditioned 12 hours before surgical incisions were made. Laser protocols were optimized using temperature, blood flow, and hsp70-mediated bioluminescence measurements as benchmarks. Bioluminescent imaging studies in vivo indicated that an optimized laser protocol increased hsp70 expression by 15-fold. Under these conditions, healed areas from incisions that were laser-preconditioned were two times stronger than those from control wounds. Our data suggest that these methods can provide effective and improved tissue-preconditioning protocols and that mild laser-induced heat shock that correlated with an expression of Hsp70 may be a useful therapeutic intervention prior to or after surgery.

The invention discloses an anti-microbial healing-promoting hydrogel dressing and a preparation method therefor. The main ingredients of the dressing are water-soluble high-molecular polymers, functional polymers with pseudoplastic, anti-microbial agents and water. The above ingredients are dissolved into a whole after a series of technologies. The solution is poured into a mold, and the hydrogel dressing is obtained after curing and irradiation disinfection. The results of wound healing experiments and in vitro anti-microbial experiments show that the hydrogel dressing can promote wound healing effectively, and has good bacteriostatic activity to multiple bacteria (the sterilizing rate is above 70%). The anti-microbial healing-promoting hydrogel dressing is better than existing hydrogel dressings obviously, and has advantages of substantial anti-microbial curative effects, transparency, high water content, simple preparation method, pure product, nontoxicity, innocuousness, no stimulation, good biocompatibility and the like. The hydrogel dressing is suitable for large-area promotion and application.

Compositions and methods for treatment of skin utilizing thymosin β4.

Oxygenating agents are used in the present invention for the novel purpose of treating microbial infections. The invention takes advantage of the fact that the increase in tissue pO2 produced thereby can enhance the efficacy of the body's own antimicrobial defenses (including tissue repair), while also improving the efficacy of adjunctive agents that may be co-administered, such as antimicrobial agents, antioxidants, cytokine modulators, endotoxin antagonists and growth factors. The oxygenating agents of the present invention achieve the desired increased pO2 in the tissues without having to resort to the risks and expenses of hyper-baric oxygen therapy (HBO). The oxygenating agents can be administered systemically, regionally, or topically.

The methods and compositions of this invention provide a means of regenerating injured musculoskeletal tissue by inhibition of myostatin function. The invention provides methods of treating a nonunion fracture in an individual comprising delivering to the fracture via a delivery system comprising biodegradable hydrogel, a pharmacological amount of myostatin propeptide effective to inhibit myostatin function. The invention also teaches compositions useful for the treatment of non-union fracture in an individual, said compositions comprising a pharmacological amount of myostatin propeptide effective to inhibit myostatin function; and a delivery system for delivering said myostatin propeptide to said fracture, wherein the delivery system comprises a biodegradable hydrogel or biodegradable nanobeads.

A wound dressing and method of use is provided that increases healing of tissues by targeting damaged tissue at a predetermined wavelength. The device includes the use of a negative pressure bandage, a flexible light sheet, and one or more bioactive marine extracts. Light emitted from the flexible light sheet penetrates through the bandage to target damaged tissue, which accelerates the wound healing process and works synergistically with the negative pressure bandage and bioactive marine extracts such as collagen fibers, alginate, chitosan and fucoidan, or any combination thereof to accelerate healing.

The invention provides a medical dressing for protecting skin wounds and promoting repairing, an application of the medical dressing and a preparation method of the medical dressing. The medical dressing comprises a biological plural gel film dressing and a medical polyurethane adhesive film; the biological plural gel film dressing is composed of raw materials of a framework material synthesized by polyanion substances and polycation substances, hyaluronic acid, polypeptide and bonding polypeptide; and the potential of hydrogen (pH) value of the biological plural gel film dressing is 5.8-6.4. The medical dressing for protecting the skin wounds and promoting repairing of the skin wounds has the advantages of wound covering medical dressings, more importantly, by means of synergistic actions of the bonding polypeptide and the hyaluronic acid, the structural designs of medical dressing materials are optimized, and additional growth factors or living cells are not required for enabling the medical dressing materials to have functions of in-situ inducing tissue regeneration and repair.

The invention discloses a solid phase synthesis method and a liquid phase synthesis method of chitosan and / or zinc, silver, bismuth and rare earth metal composite of chitosan derivative. The solid phase synthesis comprises the following steps: (1) grinding chitosan and / or chitosan derivative to fine powder; (2) grinding the oxides of zinc and / or silver and / or bismuth and rare earth metal to fine powder of which content is 0.0005-2wt% of the weight of chitosan and / or chitosan derivative; (3) mixing the fine powders obtained in step (1) and step (2) and fully grinding for 1-6h to obtain the chitosan and / or metal composite of chitosan derivative. The product of the preparation method can be used to cure diseases such as female vaginitis, cervicitis, cervical erosion, bedsore, skin ulcer, fireburn, empyrosis, trauma, other dermatitis disease, tinea of feet and hands and the like and can be used to prepare various surgical dressings such as film, sponge, gynecological suppository, adhesivefilm and the like.

The invention provides a degradable expandable local urine tract intracavity support system to effectively support a local lesion urine tract after urine tract stricture obstruction surgery and to prevent stricture obstruction replase due to postoperation scar hyperplasia and contracture. The support system is made of degradable material and can be degraded after scar stability and before adhesive lithogenesis formation and can be discharged with urine outside the body; and the support system comprises but is not confined to a naked rack, a drug eluting stent or a drug coating layer rack, a film covering rack, etc. The support is compressed into an exotheca with small caliber and then released after reaching a target urine tract position, then expanded and enlarged to support the lesion urine tract; the internal diameter and length of the support can be adjusted according to clinic demands; and compared with traditional double J type ureter racks and catheters, interference and damage to normal urine tract except for the stricture part can be avoided, and limitation of rack lumen inner diameter by the urine tract physiological stricture part can be avoided.

The invention belongs to the field of biological materials, and relates to a micro-patterning nano-fiber material composited with medicine-carrying MOF and a preparation method and application thereof. According to the material, hydrophilic composite electrostatic spinning nano fiber arranged in a staggered mode is adopted as a skeleton, the micro-patterning structure is arranged in order, and anopen three-dimensional through porous structure is distributed; the hydrophilic composite electrostatic spinning nano fiber is a mixture of a supporting biological compatible material, a hydrophilic biological compatible material and repair active molecule loaded metal organic frame nano-particles. The preparation method comprises the steps that organic solves of the metal organic frame nano-particles with uniformly dispersed loaded repair active molecules, the supporting biological active material and the hydrophilic biological active material are adopted as spinning solutions, and electro spinning is performed for preparation. According to the material, on the basis of the slow-release medicine carrier activity and biological tissue activity, the activity of cells in the engine body environment and micro-patterning nano-fiber material is collaborated and promoted, tissue repair and wound healing are facilitated, and the material is used for preparing a wound healing material.

The construction process of skin tissue engineering rack containing epidermal growth factor with fetus calf or ox tendo achillis as basic material includes dewatering, defatting, primary enzyme treatment to reduce the covalent bond among tissue fibers, dialysis, secondary enzyme treatment to remove antigenic determinant, dissolving with acetic acid to obtain no-antigenicity collagen solution and collagen sponge film, soaking in propanetriol to adsorb, drying, mixing water solution of heparin and acetic acid, water solution of epidermal growth factor and propanetriol wetted collagen sponge film, and final freeze drying. The rack has no antigenicity, high strength, high flexibility, convenient operation implantation, no toxicity to wound, and capacity of inducing wound cell growth and promoting wound healing.

The present invention describes the incorporation of liposomal gene constructs directly into a wound to further improve wound repair, or into wound coverage and / or closure materials to enhance the functionality of the material. The present invention further describes the use of human fetal membranes (e.g., amnion) enhanced with the liposomal gene therapy as a wound coverage material in full-thickness wound repair. The enhanced fetal membranes or enhanced cadaver skin have advantages over currently used materials lacking the liposomal gene construct and are an efficient and safe approach to improve clinical outcome in patients with burn injuries.

The invention discloses the preparation and clinical application of a fat-derived stromal cell composite graft material rich in cytokines. The preparation method is through 1) the acquisition and purification of fat granules; 2) the acquisition of nano-fat stromal cells; 3) the biological characteristics and multi-directional differentiation identification of nano-fat-derived stem cells (NFSCs); 4) fibrin glue rich in cytokines The preparation of transplant materials in four steps is used for the clinical repair and correction of sunken deformities of the face, chest and other body surface soft tissues. The invention is based on the new technology of stromal vascular components to promote trauma or wound repair, reduce scar hyperplasia, promote tissue regeneration repair and organ reconstruction, shorten the recovery time of patients, improve the quality of rehabilitation, and achieve good curative effect. It is a clinically assisted autologous fat Tissue transplantation provides experimental basis and theoretical support for the repair of soft tissue or organ defects, burn wounds, refractory wounds, and breast reconstruction after breast cancer surgery.

The invention relates to an artificial dermis bracket. The artificial dermis bracket comprises a three-dimensional porous structure with a deep layer and a shallow layer, wherein sustained release microspheres loaded with functional factors are introduced into the artificial dermis bracket, and comprise microspheres loaded with antibiotics and microspheres loaded with skin growth factors, and a gradient structure is formed by the three-dimensional porous structure of the artificial dermis bracket due to different porosities of the deep layer and the shallow layer. The preparation method of the artificial dermis bracket mainly comprises the steps of: preparing the sustained release microspheres loaded with the functional factors; preparing collagen solutions with two concentrations as matrix solutions; and mixing the sustained release microspheres loaded with the functional factors into the corresponding matrix solutions and molding the three-dimensional porous structure comprising the deep layer and the shallow layer so as to form the artificial dermis bracket with introduction of the sustained release microspheres loaded with the functional factors. The artificial dermis bracket provided by the invention has antibacterial activity and the property of promoting wound healing.

The invention discloses a composite electrostatic spinning nanofiber film and a preparation method and application thereof. The preparation method of the composite electrostatic spinning nanofiber film carrying drug controlled-release microspheres includes: (1), preparing polylactic acid-glycolic acid copolymer PLGA microspheres with basic fibroblast growth factors (bFGF) embedded; (2), respectively preparing polycaprolactone / chitosan electrostatic spinning liquid, polycaprolactone electrostatic spinning liquid and gelatin electrostatic spinning liquid; (3), using an electrospinning method to prepare a PCL / CS-PLGA microsphere-PCL / GE electrostatic spinning nanofiber film, namely the composite electrostatic spinning nanofiber film. The nanofiber film is utilized to improve bio-compatibility of PU sponge, promote tissue healing and reduce visceral wear; the microspheres are introduced to realize drug controlled-release, and many blanks of clinically-existing VAC can be filled.

The present invention relates to construction process of artificial skin containing human bone marrow mesenchymal stem cell. Skin tissue engineering rack is first constructed with fetus calf or ox tendo achillis as basic material, collagen sponge film through twice enzyme treatments and the mixture of heparin and epidermal growth factor; and human bone marrow mesenchymal stem cell obtained via purifying human bone marrow blood is implanted on two sides of the rack to constitute the artificial skin with bone marrow mesenchymal stem cell on the two sides of and in the internal of the rack. The artificial skin has no antigenicity, high strength, high flexibility, no toxicity to wound, and capacity of inducing wound cell growth and promoting wound healing.

The invention belongs to the technical field of medication, in particular to a biological preparation for treating and restoring an infective wound surface and a preparation method thereof. The preparation disclosed by the invention comprises the following components in percentage by weight: 0.1 to 30 percent of staphylococcus lysozyme, 3 to 50 percent of chitosan or chitosan derivative and 30 to95 percent of collagen. The preparation method comprises the following steps: crosslinking the natural biodegradable chitosan derivative and the collagen serving as dressings to form hydrogel used asa carrier; and loading the staphylococcus lysozyme into the hydrogel to prepare a novel preparation with a function of promoting the healing of the wound surface and a bactericidal effect.

A wound dressing and method of use is provided that increases healing of tissues by targeting damaged tissue at a predetermined wavelength. The device includes the use of a negative pressure bandage, a flexible light sheet, and one or more bioactive marine extracts. Light emitted from the flexible light sheet penetrates through the bandage to target damaged tissue, which accelerates the wound healing process and works synergistically with the negative pressure bandage and bioactive marine extracts such as collagen fibers, alginate, chitosan and fucoidan, or any combination thereof to accelerate healing.

The invention provides a hyaluronic acid and silica gel composition for scar prevention and early repair and a preparation method of the hyaluronic acid and silica gel composition. The method includes the steps of adding purified water to hyaluronic acid to be evenly stirred to prepare a hyaluronic acid solution, adding purified water to onion extract to prepare an onion extract solution, adding carbomer and purified water to glycerol to be evenly stirred to prepare a carbomer solution, sequentially adding the hyaluronic acid solution and the onion extract solution to the carbomer solution to be stirred, adding polydimethylsiloxane and dimethyl siloxane cross-linked polymers to be evenly stirred, making the mixture pass through a colloid mill, and obtaining the hyaluronic acid and silica gel composition. By adding natural plant extract for promoting skin wound healing, natural moisturizing factors, namely, hyaluronic acid, and siloxane polymers, the good effect of preventing scars from being formed is achieved, the effect of rapidly repairing scars in the early stage is achieved, and good effects are achieved for prevention and early repair of scars caused by general operations, traumas and burns.

The invention relates to a temperature-sensitive type skin wound dressing as well as a preparation method and applications of the temperature-sensitive type skin wound dressing. The temperature-sensitive type skin wound dressing is prepared from the following components in percentage by weight: 1-20 % of biological active glass, and 80-99 % of temperature-sensitive type composite hydrogel, wherein the temperature-sensitive type composite hydrogel is prepared from the following components in percentage by weight: 0.5-2 % of alginate, 0.5-2 % of agar and / or agarose, the balance of water. The temperature-sensitive type skin wound dressing is composed of the temperature-sensitive type composite hydrogel and the biological active glass, is in the solution state at high temperature, can form hydrogel at the temperature close to the body temperature, has a certain bacteria inhibiting effect, and has an acceleration effect for the wound repair.

The invention relates to a wound repairing material which comprises an upper-layer stent and a lower-layer stent, wherein the upper-layer stent comprises a polyester fiber layer, the lower-layer stent comprises a polyester layer with a three-dimensional porous membrane structure, and natural high-molecular materials rich in protein and / or polysaccharide are compounded in the pores. A preparation method of the wound repairing material comprises the steps of preparing the lower-layer stent by combining a particle filtering process with a freeze-drying process; spinning on the lower-layer stent by an electrostatic spinning process to obtain the upper-layer stent. The wound repairing material provided by the invention has an effect on imitating the fetal skin type, and can effectively reduce the wound shrinkage to promote quick healing of skin and inhibit scar hyperplasia and contracture to achieve the ultimate goal of traceless healing.

The invention provides a human placenta extract, a method for preparing the same and application of the extract in the aspect of skin care products and belongs to the field of placenta extracting preparation and application. The problems that a current human placenta extract preparation method is complex, causes great damage to beneficial substances in the placenta and is not suitable for applying the human placenta extract to the field of skin care products. According to the human placenta extract, the method for preparing the same and the application, after being cut up and washed, the human placenta is stirred and extracted under the conditions of the special pH in a special extraction buffer solution, the obtained human placenta extract contains various active nutritional components such as high-concentration cell factors, lecithin, polysaccharide and amino acids. Skin care products prepared by taking the human placenta extract as the main raw material to be matched with bioactive protective agents and various plant essences, humectants and stabilizers have very good effects of skin care, wound healing, allergy resistance, anti-inflammation, wrinkle reduction and stain fading.

The invention comprises 1) an oral composition, including an oral rehydration composition or solution, comprising an effective amount of a compound selected from epidermal growth factor (EGF) and agonists to the epidermal growth factor receptor, 2) a kit comprising an oral rehydration composition containing an effective amount of a compound selected from epidermal growth factor and agonists to the epidermal growth factor receptor, and 3) methods for the treatment and prevention of dehydration and diarrhea, and enhancing intestinal healing, reducing bacterial colonization, reducing the incidence of weight loss, increasing food uptake, enhancing rehydration, and enhancing mucosal healing in an animal having diarrhea.

The invention discloses polysaccharide biomedical colloid. The colloidal fluid is prepared from, by weight, 0.5-10% of sodium carboxymethyl cellulose, 0.5-1.5% of sodium chloride, 0.5-1.5% of carbomer, 0.08-0.7% of sodium hyaluronate, 0.01-0.05% of phytate, 0.02-0.1% of sodium alginate, 0.1-0.3% of glycerol, 0.15-0.45% of chitobiose, 0.15-0.35% of polyhexamethylene guanidine, 0.01-0.2% of liquorices extract and the balance water used for injection. By means of formula optimization, the polysaccharide biomedical colloid is free of toxic and side effects, has great clinical application value, is short in healing time and high in healing rate, forms a wound surface wet environment, can directly restrain scar forming when used for cell repairing, and can form a wound surface protection film, block injuries caused by bacteria to wound surfaces, and restrain the adsorption process, the multiplication process, the copying process and other processes of bacteria on wound surfaces.

The invention relates to a composite type artificial dura mater, comprising two hydrogel layers and a woven layer, wherein the woven layer is arranged between the two hydrogel layers, the woven layer is a degradable polymer material, and cell growth factors are contained in the hydrogel layers. The composite type artificial dura mater disclosed by the invention has the following beneficial effects: (1) the artificial dura mater is made from the degradable polymer material and biological protein which are easily available, and has good biocompatibility; (2) atmospheric pressure plasma jet treatment increases the fiber surface area of the woven layer, decreases a contact angle of a material surface, and improves water transmissibility, degradation speed and cellular affinity; (3) regulation on physical characteristics of the artificial dura mater, such as malleability, toughness, mechanical strength and the like can be realized by regulating a weaving method; and 4) the composite type artificial dura mater is favorable for wound repair, and silver ions enable artificial skin to have good antibacterial performance, so that toxic and side effects on a human body due to the use of a chemical antibacterial medicine are avoided, and storage life of the artificial skin is prolonged.

The invention discloses an xenogenic dermal reticular layer scaffold with basilar membrane removal and no cells, which is taken from an dermal reticular layer of a mammal, and has a natural three-dimension structure with larger pores of the dermal reticular layer; the dermal scaffold removes a basilar membrane and has no cells; and the thickness of the dermal scaffold is between 0.2 and 0.8mm. The scaffold is prepared by performing sterile dermis getting, then removing subcutaneous tissue, epidermis, the basilar membrane and a dermal papillary layer, then orderly using distilled water to soak and using trypsinase and an EDTA.Na2 digestive juice to digest, using a phosphate buffer solution to wash after the persistent oscillation, then placing into a Triton X-100 solution for soaking and performing the persistent oscillation, and then using the phosphate buffer solution to wash. The scaffold completely removes the source of a main immunogenic substance in dermis, and reduces the immunogenicity; the scaffold has strong nutrition permeability, quick vascularization speed, high survival rate and good effect when used for the repair of skin wounds, the construction of tissue engineering skin, and tissue filling; and the scaffold has the advantages of rich dermis sources, simple preparation technology, short production period, and low cost.

The invention relates to a hyaluronic acid bacteriostatic gel, which comprises the following raw materials by weight: 50-60 parts of hyaluronic acid, 30-40 parts of a Chinese medicinal extract, 15-20 parts of DCBA, 3-5 parts of ethanol, 5-8 parts of glycerol, and 10-15 parts of purified water. The Chinese medicinal extract includes the following raw materials: Ampelopsis japonica, radix angelicae dahuricae, white poria, kudzuvine root, trichosanthes root, bighead atractylodes rhizome, dandelion, forsythia, honeysuckle, radix paeoniae rubra, cortex moutan, sophora flavescens and licorice. The hyaluronic acid bacteriostatic gel provided by the invention has bacteriostatic effect on staphylococcus aureus, escherichia coli, and pseudomonas aeruginosa, can be daubed on the skin to form a skin protective film, and is suitable for skin bacteriostasis, bacteriostatic protection postoperative skin wounds and daily skin care.

The invention relates to a proliferation accelerant and application thereof, in particular to a fibroblast proliferation accelerant capable of promoting fibroblast proliferation and fibroblast extractand fibroblast extract freeze-dried powder prepared through fibroblasts. The proliferation accelerant comprises loofah saponin and / or momordica saponin. A loofah saponin and momordica saponin combined inducer is adopted for culture of skin fibroblasts, so that synthesis and secretion of bioactive substances derived from the skin fibroblasts are promoted effectively, content of active ingredientsin cell derivatives is increased, and production efficiency and biologic effect of the skin fibroblast derived cell derivatives.

The invention relates to a dandelion and honeysuckle acne removing face mask and a production method thereof, relating to the technical field of daily necessaries. The dandelion and honeysuckle acne removing face mask is produced by adopting the following raw materials: 80-100g of dandelion, 20-30g of honeysuckle, 30-50g of mung bean and 10-15g of honey. The production method comprises the following steps of decocting the amount of dandelion and the honeysuckle with water, filtering, remaining 500-600ml of filtrated juice, adding the amount of the mung bean, boiling with water for 1 to 3 hours, and adding the amount of honey. According to the technical scheme, the dandelion has the effects of clearing away heat and toxic materials and a broad-spectrum antibacterial effect, and the honeysuckle has the effect of clearing heat and diminishing inflammation, so that the face mask can be used for effectively treating acnes. The matched mung bean can be used for enhancing the curative effect of clearing heat and diminishing inflammation. The matched honey can be used for flavoring and enhancing the antisepsis and anti-inflammation effect of the dandelion. After being applied on a face, the dandelion and honeysuckle acne removing face mask has direct dirt removal and bactericidal effect on the skin and also has nutrition and caring effects.