Human placenta extract, method for preparing the same and application

A technology of extract and human placenta, which is applied in the field of placenta extraction, preparation and application, can solve the problems of insufficient utilization, high cost, complicated process, etc., and achieve the effects of small damage, reducing wrinkles and avoiding immune reaction

Inactive Publication Date: 2015-09-30
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the freeze-dried powder of this extract has cosmetic effects such as "prolonging puberty, beautifying skin, increasing life expectancy, and delaying aging", this freeze-dried powder is an extract of stem cells isolated and purified from human placenta after cultivation, and has no Make full use of other beneficial substances in the placenta, and also need to separate and purify stem cells, the process is complicated and the cost is high

Method used

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  • Human placenta extract, method for preparing the same and application
  • Human placenta extract, method for preparing the same and application
  • Human placenta extract, method for preparing the same and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of human placenta extract, comprising the following steps:

[0037] 1. Before the parturient gives birth, reach a preparation agreement with the parturient, collect and quickly freeze at the delivery site;

[0038] 2. Take the thawed placenta and wash away most of the blood clots in the placenta repeatedly with deionized water;

[0039] 3. Under the condition of 4℃-25℃, use surgical scissors to cut into 1cm 3 size;

[0040] 4. Under the condition of 4°C-25°C, take 1L of normal saline to soak and wash for 2-5min, and wash repeatedly for a total of 5-10 times. When the tissue block turns to flesh-colored, it means that it has been cleaned;

[0041] 5. Drain the washed tissue pieces with gauze, add 1L of extraction buffer solution, and pulverize with a homogenizer at 4°C-25°C (rotating speed is 8000rpm-12000rpm), and the mixture A obtained after crushing is in the form of porridge liquid; by weight, the extraction buffer solution contains 80-99 pa...

Embodiment 2

[0050] With the human placenta extract that obtains in embodiment 1, the active ingredient wherein is detected and analyzed:

[0051] Molecular weight detection: SDS-PAGE electrophoresis to detect the molecular weight of human placenta extract

[0052] The human placenta extract obtained in Example 1 was diluted 3 times with protein loading buffer, and then divided into two parts as parallel samples. SDS-PAGE protein electrophoresis was used to detect the molecular weight of each substance in the human placenta extract. For the test results, see figure 1 .

[0053] figure 1 Among them, M represents the protein maker, 1 and 2 respectively represent the parallel samples averaged into two; figure 1 The number on the left indicates the molecular weight of each protein marker. from figure 1 It can be seen from the figure that using the method of Example 1, the extracted human placenta extract contains a variety of human proteins, and the molecular weight of each protein is g...

Embodiment 3

[0064] With the human placenta extract obtained in embodiment 1, detect its effect of suppressing melanin secretion, concrete operation is as follows:

[0065] The human placenta extract was co-incubated with mouse melanoma cell B16, and the inhibitory effect of the placenta extract on melanin secretion of melanoma cells was tested. The results are shown in figure 2 shown.

[0066] figure 2 ABCD in represents the inhibition results of placental extracts in different amounts added at the same culture time, wherein,

[0067] A means culturing with 2ml serum medium with a mass concentration of 10% for 96 hours; B means culturing with 0.5ml placenta extract + 1.5ml serum-free medium for 96 hours; C means culturing with 1.0ml placenta extract + 1.0ml serum-free culture medium for 96 hours; D means culture with 1.5ml placenta extract + 0.5ml serum-free medium for 96 hours.

[0068] from figure 2 It can be seen that the human placenta extract obtained in Example 1 has a dose-g...

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Abstract

The invention provides a human placenta extract, a method for preparing the same and application of the extract in the aspect of skin care products and belongs to the field of placenta extracting preparation and application. The problems that a current human placenta extract preparation method is complex, causes great damage to beneficial substances in the placenta and is not suitable for applying the human placenta extract to the field of skin care products. According to the human placenta extract, the method for preparing the same and the application, after being cut up and washed, the human placenta is stirred and extracted under the conditions of the special pH in a special extraction buffer solution, the obtained human placenta extract contains various active nutritional components such as high-concentration cell factors, lecithin, polysaccharide and amino acids. Skin care products prepared by taking the human placenta extract as the main raw material to be matched with bioactive protective agents and various plant essences, humectants and stabilizers have very good effects of skin care, wound healing, allergy resistance, anti-inflammation, wrinkle reduction and stain fading.

Description

technical field [0001] The invention belongs to the field of extraction, preparation and application of placenta, and in particular relates to a human placenta extract, a preparation method thereof, and an application of the extract in skin care products. Background technique [0002] The placenta is a transitional organ for exchanging substances between mother and child formed by the joint growth of the embryonic membrane and the maternal endometrium during the pregnancy of metatherians and eutherian mammals. The fetus develops in the womb, relying on the placenta to obtain nutrition from the mother, while both parties maintain considerable independence. The placenta also produces a variety of pregnancy-maintaining hormones and is an important endocrine organ. In China, the placenta after childbirth is also a kind of traditional Chinese medicine, which is called human afterbirth and Ziheche. [0003] Generally, the main components of placenta include proteins, nucleic aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61Q19/00A61Q19/02A61P37/08A61P29/00
Inventor 胡忠国海泉陈静娴高雪华孙雯廖知恒赵峻
Owner CHENGDU QINGKE BIOTECH
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