The invention discloses a rapid preparation method of
umbilical cord-derived mesenchymal stem cells, and relates to the technical field of preparation of
umbilical cord-derived mesenchymal stem cells, and the rapid preparation method comprises the following experimental steps: step 1, taking a fresh healthy
umbilical cord of 16 + / -1cm, equally dividing the umbilical cord into two parts by using
surgical scissors in a culture dish, respectively putting the two parts into a new culture dish, and marking the two parts as a group A; 2, 75% medical
alcohol is sprayed to the umbilical cord A, and
saline water is used for flushing after standing is conducted for 30 s; step 3, extracting the group A by using a straight pair of surgical
forceps, a bent pair of surgical
forceps, a left pair of surgical
forceps and a right pair of surgical forceps, discharging blood in blood vessels in an
extrusion manner, washing the blood by using
saline water, putting the umbilical cord tissues into a 50ml centrifugal tube, and fully
cutting the umbilical cord tissues into blocks with the size of 1.5 + / -0.1 mm by using
surgical scissors for later use; according to the rapid preparation method of the umbilical cord source mesenchymal stem cells, blood vessels do not need to be removed, growth of vascular endothelial cells is prevented, and purity uniformity and biological
efficacy of the mesenchymal stem cells are guaranteed to meet industrial requirements.