Preparation and clinical applications of cytokine-rich fat-derived stromal cell composite transplantation material

Abstract

The invention discloses the preparation and clinical application of a fat-derived stromal cell composite graft material rich in cytokines. The preparation method is through 1) the acquisition and purification of fat granules; 2) the acquisition of nano-fat stromal cells; 3) the biological characteristics and multi-directional differentiation identification of nano-fat-derived stem cells (NFSCs); 4) fibrin glue rich in cytokines The preparation of transplant materials in four steps is used for the clinical repair and correction of sunken deformities of the face, chest and other body surface soft tissues. The invention is based on the new technology of stromal vascular components to promote trauma or wound repair, reduce scar hyperplasia, promote tissue regeneration repair and organ reconstruction, shorten the recovery time of patients, improve the quality of rehabilitation, and achieve good curative effect. It is a clinically assisted autologous fat Tissue transplantation provides experimental basis and theoretical support for the repair of soft tissue or organ defects, burn wounds, refractory wounds, and breast reconstruction after breast cancer surgery.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the preparation and clinical application of a fat-derived stromal cell composite graft material rich in cytokines. Background technique [0002] Depression and deformity of body surface tissues and organs due to trauma, infection, tumor resection or congenital defects, etc., not only affect the appearance, but also affect the patient's mental health and social communication activities to varying degrees. Autologous fat grafting is currently one of the main methods for the treatment of soft tissue depressions or asymmetrical contours. Its main disadvantage is that it is easy to absorb after transplantation, and the absorption rate accounts for 20% to 90%. Most of them need to receive repeated injections to achieve a satisfactory effect. research hotspots. [0003] Adipocytes lack effective blood supply in the early stage of transplantation. Before the blood suppl...

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Problems solved by technology

Its main disadvantage is that it is easy to absorb after transplantation, and the absorption rate accounts for 20% to 90%. Most of them need to receive repeated injections to achieve a satisfactory effect. research hotspots

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