63 results about "Schizophyllan" patented technology

The invention relates to the field of personal care, in particular to a schizophyllan-containing additive for cosmetics. The schizophyllan-containing additive for cosmetic is prepared in parts by weight: 0.5 to 2 parts of schizophyllan, 0.5 to 4 parts of a polyhydric alcohol, 0.1 to 0.7 parts of a thickener, and 90 to 100 parts of water; and the polyhydric alcohol is selected from one or more of polyhydric alcohols from C2 to C12. The schizophyllan-containing additive for cosmetic can be applied to a variety of cosmetics, when being applied to hair care products, problems such as hair fallingand dry and itchy scalp can be improved and solved, the schizophyllan-containing additive for cosmetic can also be used in cleaning and wash supplies and skin care products, and immunity and repair ability of the skin tissues are improved; in addition, the long-term solubility and stability of the schizophyllan-containing additive for cosmetic at low temperatures is improved by adding the polyhydric alcohols and the amino acids, and activity of the schizophyllan can be maintained for a long time.

The invention discloses a phosphorylated derivative of schizophyllan. The phosphorylated derivative of schizophyllan is prepared from, by mass, 1 to 10% of schizophyllan ad 1.5 to 20% of a phosphorylation reagent, with the balance being a solvent. The invention also discloses a preparation method for the phosphorylated derivative of schizophyllan. The preparation method comprises the following steps: (1) adding schizophyllan and the phosphorylation reagent into the solvent and carrying out mixing and a stirring reaction; and (2) subjecting a reaction solution obtained after the reaction in thestep (1) to separation and purification so as to obtain the phosphorylated derivative of schizophyllan. The phosphorylated derivative of schizophyllan has the advantages of good instant and long-lasting effect when used as an antioxidation active component, a humectant and an anti-aging active component for preparation of external agents for skin.

The invention discloses a method for regulating the molecular weight of schizophyllan as a fermentation product of schizophyllum commune franch. The method comprises the following steps: taking slopestrains of schizophyllum commune franch, and carrying out slope inoculation and culture by adopting a PDA culture medium test tube, thereby preparing fermentation seed liquid; inoculating the seed culture liquid into a fermentation tank for fermentation, wherein the initial stage of fermentation is 0-36 hours, and the fermentation conditions are controlled as follows: the stirring rotation speed is 100-400r / min, the ventilation volume is 0.20-0.8vvm, and the temperature is 26-28 DEG C; the middle stage of fermentation is 37-84 hours, and the later stage of fermentation is 85-96 hours; and respectively controlling the fermentation conditions, discharging after the fermentation is ended, and removing mycelia; concentrating, precipitating, filtering to obtain refined schizophyllan. Accordingto the method disclosed by the invention, polysaccharides are degraded by utilizing enzyme self-secreted by the thalli, so that not only is the procedure of degrading after separating and purifying crude polysaccharides with larger molecular weight and poorer solubility and obtained by fermentation in the traditional method greatly simplified, but also the product yield is increased and the production cost is reduced.

The invention discloses a schizophyllan extract and a preparation method thereof in the field of biological pesticides. The method comprises the following steps of: adding schizophyllum commune hypha powder into hot water in a ratio, performing extraction for 8 to 10 hours in a water bath of 100 DEG C, filtering the extract by using gauze, extracting the obtained filter residue again by using thesame method, combining the filtrate of two times, performing vacuum concentration, mixing the concentrate and 95 percent ethanol in an amount which is 5 times volume of the concentrate, standing the mixture over night at the temperature of 4 DEG C, centrifuging, washing the sediment for 2 times by using ethanol, drying the sediment at the temperature of 60 DEG C, and thus obtaining the schizophyllan extract. The invention also discloses application of the schizophyllan extract in prevention and control of tobacco mosaic virus diseases. The schizophyllan extract has good prevention and controleffect on tobacco mosaic viruses, the tobacco mosaic virus inhibiting rate can reach over 70 percent, and a new path is provided for prevention and control of the tobacco mosaic virus diseases; the schizophyllan extract is a natural extract, and is harmful to the human being and the environment; and moreover, the preparation method is simple and low in cost.

The invention provides a macro fungus, namely white ginseng, derived from coastal mudflat; the macro marine fungus is identified to be schizophyllum commune which is capable of growing in a high-salinity culture medium and is capable of generating schizophyllan. The marine schizophyllum commune disclosed by the invention is screened by virtue of a natural seawater prepared restrictive medium by taking rotten woods from coastal and seaside wetlands as samples; and the schizophyllum commune is subjected to liquid fermentation, so that the marine schizophyllan is obtained. The chemical component of the schizophyllan produced by the invention is beta-1,3 / 1,6-glucan, which is derived from the marine schizophyllum commune and is applicable to the fields of food, cosmetics and medicines; and the schizophyllan is suitable for industrial production and is quite high in application and popularization values.

The invention discloses a hair lash growth liquid containing schizophyllan, rehmannia Chinensis root and a pine extract, which contains deionized water, butanediol, ethanol, schizophyllan, a Chinese rehmannia Chinensis root extract, a sophora flavescens root extract, hydroxypropyl cyclodextrin, glycerin, sodium metabisulfite, glycine, an European larch extract, zinc chloride, a tea extract, oligopeptide-1, propylene glycol, capryloyl hydroxamic acid, glycerol caprylate, 1,2-hexanediol, p-hydroxyacetophenone, a cacumen biotae extract, a Chinese honey locust extract, and a fleece-flower root extract, The liquid contains a plurality of natural plant extracts, the product is natural and mild, the spraying of the growth liquid can greatly improve the mental appearance of the user, the white hair and the eyelashes gradually turn black, the density of hair and eyelashes can be increased, the regrowth of the loss can be stimulated, and alopecia areata can be significantly improved.

The invention belongs to the technical field of cosmetics, and in particular relates to a composition with functions of skin repairing and age defying, and a preparation method of the composition. Theskin care composition with the functions of skin repairing and age defying is prepared from the following components in parts by mass: 33-47 parts of chitosan oligosaccharide, 10-20 parts of acylatedchitosan oligosaccharide, 20-30 parts of chitosan, 22-38 parts of carboxymethyl chitosan, 10-15 parts of beta-glucan and 3-6 parts of schizophyllan. The composition with the functions of skin repairing and age defying is safe to use, mild in properties and free from skin irritation, and does not cause skin allergy. Furthermore, the composition also can replenish skin cell nutrients so as to achieve the effects of deeply repairing, promoting cell metabolism and activating cell vitality; the composition also has better anti-bacterial, moisturizing and anti-aging functions for the skin.

The invention relates to a schizophyllan liposome as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving a lipid substance in anorganic solvent, and removing the organic solvent to form a lipid membrane; (2) mixing the lipid membrane with schizophyllan, a surfactant and a buffer solution, and performing hydration until the lipid membrane is separated to form a milk white liquid, so as to obtain a crude suspension of schizophyllan lipidosome; and (3) carrying out ultrasonic treatment on the crude suspension of the schizophyllan liposome, and performing filtering with a filter membrane to obtain the schizophyllan liposome. The schizophyllan serving as a macromolecular and water-soluble natural product is added into cosmetics and cannot well permeate into and stay in the skin in a free state, so that the moisturizing or anti-aging effect of the schizophyllan is limited. The schizophyllan lipidosome is wrapped in lipidosome to prepare a the schizophyllan lipidosome product which is more beneficial to penetration and absorption by skin and retention in the skin, so that the utilization rate of the schizophyllan lipidosome product by skin is remarkably improved, and better moisturizing and anti-aging effects are achieved.

The invention provides schizophyllan modified sodium hyaluronate microsphere gel, a preparation method of the sodium hyaluronate microsphere gel, and application of the sodium hyaluronate microspheregel in cosmetics. The method comprises the following steps: adding dried sodium hyaluronate powder into a hyaluronidase solution, and performing enzymolysis to obtain a sodium hyaluronate small-molecule solution; slowly adding the sodium hyaluronate small-molecule solution into a mixed oil phase, slowly stirring, adding a mixed cross-linking agent of calcium chloride and divinyl sulfone, cross-linking and curing to obtain sodium hyaluronate microspheres; adding the sodium hyaluronate microspheres and pre-treated schizophyllan into an alkaline solution containing polyethylene glycol, uniformlystirring, regulating the pH value of the system to be neutral, adding a phosphate buffer solution, standing and aging, thereby obtaining the schizophyllan modified sodium hyaluronate microsphere gel.The schizophyllan modified sodium hyaluronate microsphere gel prepared by the method disclosed by the invention is small in particle size, easily penetrates into the deep part of skin, can form tiny gel with protein-water, achieves the effect of deeply moisturizing for a long time, and has excellent moisture retention property.

The invention relates to repair essence and a preparation method thereof. The repair essence comprises the raw material components of water, a hydrating agent, a thickening agent, a mussel extract, caprylhydroxamic acid, ethylhexylglycerin, schizophyllan, hydroxyacetophenone, 1, 2-hexanediol, a cork tree bark extract, a rhizoma coptidis extract, a radix sophorae yellowish sophora extract, an acardiaceae rhus chinensis insect gall extract, a common cnidium fruit extract, a Chinese prickly ash peel extract and a redleaf litse root and / or stem extract. The repair essence contains various naturalcomponents, when the repair essence is used for a long term, the water holding capacity of skin and muscle can be improved, the drying and sensitive symptoms can be effectively relieved, the inflammation can be alleviated, and the couperose skin can be improved. The repair essence is good to absorb, the components are safe and non-irritating, and the preparation method is simple, and is suitable for promotion and application.

The invention discloses a method for preparing rare ginsenoside by using schizophyllum commune for biotransformation of ginsenoside. A novel method is used for biotransformation of ginsenoside, duringa fermentation process, schizophyllum commune is capable of degrading ginsenoside to generate a lot of rare saponins, a lot of various biologically-active substances such as schizophyllan, polypeptide, alkaloid, enzyme, amino acid, and vitamin can be simultaneously generated; and health care and medicinal efficacy of a fermentation product can be increased with a maximum. For biotransformation ofginsenoside, during the fermentation process, schizophyllum commune can degrade ginsenoside to generate a lot of rare saponins, a lot of various biologically-active substances such as schizophyllan,polypeptide, alkaloid, enzyme, amino acid, and vitamin can be simultaneously generated; and health care and medicinal efficacy of a fermentation product can be increased with a maximum. The method canbe used for preparing a monomer rare saponin, and can be directly extract a crude extract product for product exploitation, a simple processing purpose with high efficiency, safety, environment protection, and low cost can be achieved, and the method provides guarantee for industrial production, and food and new drugs preparation.

Disclosed is a gene carrier composed of a hydrogen-bonding polymer having a polymer chain with hydrogen-bonding sites formed on the polymer chain, in which the polymer chain has a conformation similar to the conformation of a nucleic acid, and particularly, the polymer chain has helix parameters similar to the helix parameters of a nucleic acid of a helix structure. In a preferred embodiment, the hydrogen-bonding polymer is composed of β-1,3-glucan such as schizophyllan. The carrier can be expected to be applied as a vector and a separator for nucleic acids because it is capable of interacting with a nucleic acid to form a complex with the nucleic acid and thus carrying the nucleic acid.

The invention discloses a method for degrading and modifying schizophyllan by using self-produced endonuclease of a schizophyllum commune Fr. fermentation system. The method comprises the steps of firstly, carrying out revaccination, activation and culture on a slant strain of schizophyllum commune Fr. by using a PDA culture medium to obtain fermented seed liquid; inoculating in the fermented seedliquid, continuously culturing and fermenting under the conditions that the stirring speed is 100-250rpm, the ventilation rate is 0.2-0.7vvm and the culture temperature is 26-29 DEG C; then, carryingout ceramic microfiltration membrane separation, modifying polysaccharide in filtrate by means of enzymolysis, and separating the modified polysaccharide to obtain the modified polysaccharide. Afterthe method is adopted, the molecular weight of the modified polysaccharide is reduced to 1500kDa or less from 4000kDa or more by means of degradation, and the modified polysaccharide can be completelydissolved in distilled water within 24h. The method provided by the invention utilizes enzyme secreted by the strain to degrade the polysaccharide, thus not only solving the problem that specific enzyme is difficult to obtain, but also greatly simplifying the technology and lowering the production cost; therefore, the method for obtaining the soluble polysaccharide has industrial potential.

Disclosed is a new type of immunostimulating agent including an immunostimulating oligonucleotide complexed with a carrier which is safe and has a high transfection effect. The carrier complexed with the immunostimulating oligonucleotide to form the immunostimulating agent is a polysaccharide having β-1,3-bonds (preferably β-1,3-glucan such as schizophyllan). A preferred example of the immunostimulating oligonucleotide is one containing an unmethylated CpG motif. The polysaccharide for use is preferably modified with nucleic acid-binding functional group and / or cell membrane-affinitive functional group.

The invention relates to the technical field of schizophyllan extraction, and discloses a method for separating and purifying schizophyllan by two aqueous phase extraction. The method comprises the following steps of preparing schizophyllum fermentation solution and performing extraction by using a sodium carbonate / ethyl alcohol dual water phase extraction system. The schizophyllan extracted by the method has the advantages that the structure is complete; the schizophyllan yield is high; the protein removal rate is high; the operation is simple and convenient; the toxicity is low; the safety is realized and the industrial scale preparation is easy.

The invention discloses a method for preparing schizophyllan and a special culture medium. The composition of the culture medium is as follows: every kilogram of culture medium contains 400-450g of corn cob, corn stalk or corn bran, 50-100g of wheat bran, rice bran or bagasse, 0.05-0.1g of growth-promoting factor VB1, L-glutamic acid 0.05-0.1g, the rest is water. The method for preparing schizophyllan by solid fermentation of the present invention uses agricultural and by-product resources (corn stalks, corncobs, corn bran, wheat bran, bagasse, etc.) rich in fiber substrates as raw materials for fermentation, greatly reducing the raw material cost, and realized the efficient transformation of agricultural and sideline product resources, which is both environmentally friendly and economical; the method has a high yield of schizophyllan, and the polysaccharide content in the fermentation raw material can reach 4.5-5.8% (mass percentage content) .

The invention discloses a composition with long-acting moisturizing and whitening functions. The composition is prepared from the following components in percentage by weight: 0.1-10% of schizophyllan, 0.01-1.0% of phenylethyl resorcinol, 0.01-10% of glycyrrhizic flavone and the balance of water. The composition disclosed by the invention has the beneficial effects that schizophyllans with two molecular weight ranges are selected for combination with the whitening components, i.e., the phenylethyl resorcinol and the glycyrrhizic flavone, the schizophyllan with small molecular weight provides instantaneous moisturizing, the schizophyllan with large molecular weight wraps the whitening components, i.e., the phenylethyl resorcinol and the glycyrrhizic flavone, in a three-dimensional network structure, and then the whitening components can be gradually released, so that the lasting time of the whitening effect for the skin is longer, the composition is endowed with the long-acting moisturizing and whitening functions, and can provide lasting and obvious moisturizing and whitening effects for the skin; the composition can be used for preparing cosmetics with moisturizing and whitening functions and has the advantages of good instantaneous moisturizing effect and lasting moisturizing and whitening effects.

Disclosed is a new type of immunostimulating agent including an immunostimulating oligonucleotide complexed with a carrier which is safe and has a high transfection effect. The carrier complexed with the immunostimulating oligonucleotide to form the immunostimulating agent is a polysaccharide having β-1,3-bonds (preferably β-1,3-glucan such as schizophyllan). A preferred example of the immunostimulating oligonucleotide is one containing an unmethylated CpG motif. The polysaccharide for use is preferably modified with nucleic acid-binding functional group and / or cell membrane-affinitive functional group.

An object of the present invention is to provide a highly stable nucleic acid-polysaccharide complex of an siRNA and schizophyllan. A nucleic acid-polysaccharide complex is formed by adding polydeoxyadenine in which at least part of the phosphodiester link portion is phosphorothioated to an siRNA and allowing the siRNA and schizophyllan to form a complex.

An object of the present invention is to provide a highly stable nucleic acid-polysaccharide complex of an siRNA and schizophyllan. A nucleic acid-polysaccharide complex is formed by adding polydeoxyadenine in which at least part of the phosphodiester link portion is phosphorothioated to an siRNA and allowing the siRNA and schizophyllan to form a complex.

The invention discloses an anti-aging and anti-wrinkle composition and a preparation method thereof as well as cosmetics and a preparation method thereof, and relates to the technical field of skin care products, the anti-aging and anti-wrinkle composition comprises the following components: 10-30 parts of supramolecular elastin, 3-15 parts of a distiller's grain extract, 3-15 parts of a bifidus yeast fermentation product lysate, 1-10 parts of a rehmannia extract, 1-10 parts of a kapok extract, 1-5 parts of glucan and 1-5 parts of schizophyllan. 1 to 5 parts of soy isoflavone and 0.1 to 3 parts of ergothioneine. The anti-aging and wrinkle-removing composition disclosed by the invention has an excellent anti-oxidation effect, and is capable of scavenging free radicals, delaying senescence, repairing damaged collagen and collagen fibers, tightening skin and removing wrinkles; the distiller's grain extract and the bifidus yeast fermentation product lysate are rich in moisturizing factors, and deep moistening is achieved. The supramolecular elastin is obtained through modification by a supramolecular technology, so that the solubility of the elastin can be increased, the surface skin barrier is disturbed, the permeation effect is greatly improved, and the absorption of the elastin is promoted.

The invention provides a preparation method of activity-enhanced schizophyllum commune polysaccharide, which comprises the following steps: by taking schizophyllum commune sporocarp powder as a raw material, irradiating with limited high-energy electron flow, adding compound enzyme to carry out enzymolysis reaction, then carrying out subcritical deionized water extraction on enzymatic hydrolysate, and carrying out subsequent impurity removal treatment to obtain the schizophyllum commune polysaccharide. The molecular weight of the schizophyllum commune polysaccharide extracted by the method is remarkably reduced, so that the schizophyllum commune polysaccharide not only can effectively improve the diversity of intestinal flora, promote the growth of beneficial intestinal flora and inhibit the growth of potential pathogenic bacteria in intestinal tracts, but also can effectively resist the weight increase, and can be used as a natural auxiliary food which is free from side effects and is used for losing weight and regulating the intestinal flora.

Disclosed is a gene carrier composed of a hydrogen-bonding polymer having a polymer chain with hydrogen-bonding sites formed on the polymer chain, in which the polymer chain has a conformation similar to the conformation of a nucleic acid, and particularly, the polymer chain has helix parameters similar to the helix parameters of a nucleic acid of a helix structure. In a preferred embodiment, the hydrogen-bonding polymer is composed of β-1,3-glucan such as schizophyllan. The carrier can be expected to be applied as a vector and a separator for nucleic acids because it is capable of interacting with a nucleic acid to form a complex with the nucleic acid and thus carrying the nucleic acid.

The invention belongs to the technical field of gel, and discloses hydrogel for inflammatory resistance and repair. The hydrogel comprises the following components in parts by weight: 0.01-0.5 part ofan acrylic acid (ester) cross-linked polymer, 0.1-3 parts of carboxymethyl cellulose, 0.1-4 parts of hydroxyethyl cellulose, 1-20 parts of a humectant, 0.05-0.5 part of allantoin, 1-10 parts of schizophyllan, 1-10 parts of scleroglucan, 0.1-3 parts of tetrahydromethylpyrimidine carboxylic acid, 0.5-3 parts of glucolactone, 0.2-2 parts of sodium benzoate, 0.05-1 part of a pH regulator and 43-95 parts of deionized water. The invention also discloses a preparation method of the hydrogel. The hydrogel disclosed by the invention is improved in the aspects of product use mode, biocompatibility, liquid absorption, liquid feeding property, moisturizing, -inflammation resistance, repairing and the like, and the defects of a traditional product in wound nursing are overcome, so that a patient can obtain a better experience feeling in the nursing process, and wound healing is promoted more quickly.

The invention provides a fertilizer for indoor cultivation of dracaena marginata, and belongs to the technical field of dracaena marginata cultivation. The fertilizer comprises (i) ammonium salt, (ii)oxime, an additive, auxiliary materials and a stabilizer containing schizophyllan. The auxiliary materials are selected from an oligosaccharide composition with at least 20 mol% of alpha-1,6 glycosidic bonds and / or at least 20 mol% of beta-1,4 glycosidic bonds and at least one aromatic C7-C15 aldehyde; and the auxiliary materials are selected from at least one organic anhydride, at least three vitamins, at least three medium and trace elements and at least one sulfur-containing amino acid. The fertilizer has the advantages that the nitrogen content in the fertilizer is sufficient; and meanwhile, the fertilizer is rich in oxime, aldehyde, sugar, anhydride, vitamins, medium and trace elements and sulfur-containing amino acids; the liquid fertilizer does not delaminate, the sedimentation rate is small, the fertilizer is easy to store and has fewer bubbles, so that the performance of the final product is prevented from being influenced due to foam formation and / or system pressure increase.

The invention relates to hormonal face servicing essence cream and a preparation method therefor. Raw material ingredients of the hormonal face servicing essence cream comprise a mussel extract, schizophyllan, a gentian root extract, coconut oil, an oak root extract, a cork oak bark extract, urea and the like. The hormonal face servicing essence cream disclosed by the invention has the beneficialeffects that the ingredients are natural, and the hormonal face servicing essence cream is moderate and non-irritating; and in case of insisted use, the skin barrier can be enhanced, the defense capability of skin is improved, the physiological repair of the skin is promoted, the immunologic functions of the skin are improved, and thus, the action of repairing hormonal faces is exerted. The hormonal face servicing essence cream can be used for effectively repairing erubescence, edema, red spots, papillae, pustular eruption, dryness desquamation, atrophoderma thinning, angiotelectasis, pigmentation, itching, burning sensation, ache and skin tight sense caused by the hormonal faces and gradually restoring the skin to a health status; and the hormonal face servicing essence cream has a certain repair effect on the hormonal faces of various degrees, is short in repair time and is free of rebound after repair.

The invention discloses long-acting hair-blacking liquid and a preparation method thereof. The hair blacking liquid is prepared from water, benzyl alcohol, butanediol, hydroxyethyl cellulose, arginine, a polyamino acid polysaccharide condensate, xanthan gum, bis (lauramide glutamine) lysine sodium, a radix polygoni multiflori extracting solution, an angelica sinensis SINNENSIS extracting solution, a chlorella extract, a gallnut extracting solution, ethanolamine, vitamin B5, ammonia-terminated polydimethylsiloxane, sodium pyrrolidone carboxylate, sodium lactate and dipotassium glycyrrhizinate. The hair blacking liquid is prepared from schizophyllan, glycine, pyrrolidone carboxylic acid, serine, isoleucine, threonine, CI20470, PEG-40 hydrogenated castor oil, histidine, sodium benzoate, essence and EDTA (ethylene diamine tetraacetic acid) disodium, and the prepared hair blacking liquid is convenient to use, low in cost, small in dosage, quick in effect and long in effect time.

The invention discloses sulfated schizophyllan, a preparation method of the sulfated schizophyllan and application of the sulfated schizophyllan in cosmetics. The sulfated schizophyllan is prepared by the following steps: reacting infiltrated schizophyllan with pyridine sulfur trioxide, reducing the temperature to 0-20 DEG C, adding concentrated sulfuric acid to continuously react, dissolving a reaction product by distilled water, adjusting the pH value of the solution, carrying out alcohol precipitation, redissolving, dialyzing, concentrating under reduced pressure and drying, thereby obtaining the sulfated schizophyllan. The addition amount of the pyridine sulfur trioxide is controlled to be 2-6 times of the mass of the schizophyllan, the reaction temperature of the schizophyllan and the pyridine sulfur trioxide is 50-80 DEG C, and the reaction time is 1-4 hours; the addition amount of the concentrated sulfuric acid is controlled to be 1-3 times of the mass of the schizophyllan, and the continuous reaction time is 10-40 minutes. The yield of the sulfated schizophyllan prepared by the method is high and is 90-200%, the substitution degree of the product is 1.6-2.0, and the product is high in solubility and does not need to be dissolved by heating.

The invention belongs to the field of production of high-added-value products by microorganisms, and particularly relates to a fermentation culture method for producing schizophyllan from schizophyllum commune. The method comprises the following steps of: performing deep liquid culture on schizophyllum commune by using a basic culture medium, and then adding cyclopentanone fatty acid to continue culture. The fermentation culture medium and the cyclopentanone fatty acid additive provided by the invention can shorten the fermentation period of schizophyllum commune hyphae, and greatly improve the content of high-added-value products produced by unit mycelium.