Composition and beverage for improving eye health based on fermentation product and preparation method of composition and beverage

Abstract

The invention relates to the field of oral drinks, in particular to a composition and drink for improving eye health based on leavening and a preparation method of the composition and drink. 3.2 to 5.5 parts of a schizophyllum commune fermentation product; 2.1 to 4.5 parts of yeast powder containing SOD; 1.5 to 3.0 parts of sea rice leavening; 3.0 to 5.0 parts of mulberry fruit fermentation liquid; and 1.2 to 2.5 parts of a tomato extract. The yeast powder containing SOD can specifically remove superoxide anion free radicals, the mulberry fermentation liquor is rich in polyphenol, and the tomato extract has a relatively strong anti-oxidation effect and can achieve a synergistic effect, so that the ABTS free radical scavenging capacity and the microcirculation effect are remarkably improved in multiple dimensions; the sea rice leavening is rich in various trace elements and can effectively relieve asthenopia; schizophyllan contained in the schizophyllum commune leavening has a macromolecular polysaccharide spiral result, forms a gel-like substance in an aqueous solution, and can uniformly disperse and wrap each active ingredient, so that the slow-release effect of the active ingredients is improved.

Description

Technical Field

[0001] The present application relates to the field of oral beverages, and in particular to a fermentation-based composition for improving eye health, a beverage and a preparation method thereof. Background Art

[0002] Eye health issues nowadays refer to more than just visual fatigue. As awareness of eye health care gradually increases, people are not only concerned about alleviating eye fatigue, but also about dark circles and fine lines around the eyes. This is an upgraded focus of young and middle-aged people on beauty and health.

[0003] At present, the food raw materials for improving eye fatigue in China are mainly traditional Chinese medicine (such as chrysanthemum, cassia seed, wolfberry, etc.) and modern functional raw materials (such as vitamins, bilberry, lutein, etc.). These principles can improve eye fatigue through different mechanisms of action.

[0004] However, new market concerns such as dark circles and fine lines around the eyes cannot be improved. Summary of the invention

[0005] The purpose of the embodiments of the present application is to provide a fermentation-based composition, a beverage and a preparation method thereof for improving eye health.

[0006] In a first aspect, the present application provides a fermentation-based composition for improving eye health, comprising, by weight:

[0007] 3.2-5.5 parts of Schizophyllum fermentation product;

[0008] Contains 2.1-4.5 parts of SOD yeast powder;

[0009] 1.5-3.0 parts of fermented sea rice;

[0010] 3.0-5.0 parts of mulberry fermented liquid; and

[0011] 1.2-2.5 parts of tomato extract.

[0012] Visual fatigue is caused by excessive use of the eyes, staying up late, long hours of light exposure, etc., which lead to dry eyes, blurred vision, photophobia, etc. Dark circles are mainly caused by staying up late, emotional instability, eye fatigue, long-term sun exposure, insufficient oxygen supply to the red blood cells in the eye skin, chronic hypoxia, darker blood, and venous blood retention in the veins or capillaries, as well as the formation of melanin and precipitation around the eyes to form fine lines.

[0013] In the above technical solution, the various components cooperate with each other to achieve a synergistic effect, which can effectively improve visual fatigue, relieve eye fatigue and dry eyes, and can improve dark circles and lighten fine lines around the eyes, playing a role in all-round protection of the eyes. Schizophyllum polysaccharide can not only enhance eye moisturizing, relieve eye fatigue and improve the moisture of skin around eyes, but also enhance immunity and reduce the incidence of eye problems; SOD yeast powder can specifically remove superoxide anion free radicals; it can significantly improve ABTS free radical scavenging ability and improve microcirculation in multiple dimensions; mulberry fermentation liquid can promote rhodopsin regeneration, increase visual sensitivity, and reduce the recovery time of retinal macules, so as to protect eyesight and eliminate visual fatigue; at the same time, it can reduce the oxidative damage of blue light and ultraviolet rays to eyes and eye skin; it can also promote eye microcirculation; tomato extract can prevent eye skin damage caused by solar radiation; it can reduce fibrin degradation and pigmentation, improve microcirculation, and promote collagen production, thereby reducing dark circles and fine lines around eyes; sea rice fermentation helps to improve eye fatigue and dark circles caused by poor sleep quality; the combination of the above ingredients can achieve synergistic effect, improve visual fatigue in multiple dimensions, relieve eye fatigue and dry eyes, and at the same time improve dark circles and fade eye fine lines.

[0014] Furthermore, in the above technical solution, the schizophyllan contained in the fermented product of Schizophyllan has a macromolecular polysaccharide spiral, which forms a gel-like substance in the aqueous solution, and can evenly disperse and wrap each active ingredient, thereby increasing the sustained release effect of the active ingredient. When this composition is applied to oral beverages, the product can reduce its damage in the stomach and increase its absorption rate in the intestine when taken orally, thereby further improving the eye care effect.

[0015] In other embodiments of the present application, the above-mentioned parts by mass include:

[0016] 3.5-5.0 parts of Schizophyllum fermentation product;

[0017] Contains 2.5-4.0 parts of SOD yeast powder;

[0018] 1.5-3.0 parts of fermented sea rice;

[0019] 3.0-5.0 parts of mulberry fermented liquid; and

[0020] 1.2-2.5 parts of tomato extract.

[0021] In other embodiments of the present application, preparing a Schizophyllum fermentation product comprises: based on the total volume of the culture medium, inoculating 5% to 15% of the Schizophyllum seed solution into the culture medium, fermenting and culturing at 28°C to 30°C and 140 r / min to 170 r / min for 100 h to 150 h, and taking the supernatant.

[0022] In other embodiments of the present application, preparing yeast powder containing SOD includes: inoculating yeast strains into a culture medium for culturing; obtaining a fermentation product; and extracting and separating the fermentation product to obtain yeast powder containing SOD.

[0023] In other embodiments of the present application, preparing the sea rice fermentation product includes: crushing the sea rice, adding water, fermenting with brewer's yeast at 25° C. to 30° C. for 40 h to 50 h, and obtaining the fermentation liquid.

[0024] In other embodiments of the present application, preparing mulberry fermentation broth includes: based on the total mass of mulberry slurry, inoculating 0.01% to 0.04% lactic acid bacteria into the mulberry slurry, fermenting at 35° C. to 40° C. for 30 h to 48 h, and obtaining the fermentation broth.

[0025] In other embodiments of the present application, preparing the tomato extract includes: mixing tomato pulp with pectinase and cellulase, treating at 40° C. to 60° C. for 2 h to 3 h, removing pectinase and cellulase, centrifuging, and taking a precipitate.

[0026] In a second aspect, the present application provides a beverage having the effect of improving eye health, comprising the fermentation-based composition for improving eye health provided in the first aspect.

[0027] In other embodiments of the present application, the beverage does not contain a suspending agent.

[0028] In other embodiments of the present application, the dosage form of the beverage includes at least one of a beverage, soft candy, or suckable jelly.

[0029] In a third aspect, the present application provides a method for preparing a beverage having the function of improving eye health, comprising:

[0030] Dissolving SOD-containing yeast powder and sea rice fermentation product to obtain a first mixed solution;

[0031] adding mulberry fermented liquid to the first mixed liquid to obtain a second mixed liquid;

[0032] dissolving the Schizophyllum fermented product to obtain a Schizophyllum fermented product dispersion; adding tomato extract to the Schizophyllum fermented product dispersion to obtain a third mixed solution;

[0033] The second mixed solution and the third mixed solution are mixed. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0035] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0036] The present application provides a fermentation-based composition for improving eye health, which includes, by weight:

[0037] 3.2-5.5 parts of Schizophyllum fermentation product;

[0038] Contains 2.1-4.5 parts of SOD yeast powder;

[0039] 1.5-3.0 parts of fermented sea rice;

[0040] 3.0-5.0 parts of mulberry fermented liquid; and

[0041] 1.2-2.5 parts of tomato extract.

[0042] Visual fatigue is caused by excessive use of the eyes, staying up late, and long hours of light exposure, which lead to dry eyes, blurred vision, photophobia, etc. Dark circles in young people are mainly caused by staying up late, emotional instability, eye fatigue, and long-term sun exposure, which causes insufficient oxygen supply to the red blood cells in the eye skin, resulting in chronic hypoxia, darker blood, and venous blood retention in the veins or capillaries, as well as the precipitation of melanin around the eyes.

[0043] This product composition is applied in the food field, specifically relates to a composition that is beneficial to eye health and a preparation method thereof. This product can not only improve visual fatigue, relieve eye fatigue and dry eyes, but also improve dark circles and lighten eye fine lines, playing a multi-faceted role in protecting the eyes.

[0044] The present invention provides a composition and product composed of a plurality of fermented products with the function of improving eye health, which can relieve visual fatigue, improve dry eyes, and improve dark circles and fine lines around the eyes. In the above technical scheme, the various components cooperate with each other to achieve a synergistic effect, which can effectively improve visual fatigue, relieve eye fatigue and dry eyes, and can improve dark circles and lighten fine lines around the eyes, playing a multi-faceted role in protecting the eyes.

[0045] Schizophyllum polysaccharide can not only enhance eye moisturizing, relieve eye fatigue and improve the moisture of skin around eyes, but also enhance immunity and reduce the incidence of eye problems; SOD yeast powder can specifically remove superoxide anion free radicals; it can significantly improve ABTS free radical scavenging ability and improve microcirculation in multiple dimensions; mulberry fermentation liquid can promote rhodopsin regeneration, increase visual sensitivity, and reduce the recovery time of retinal macules, so as to protect eyesight and eliminate visual fatigue; at the same time, it can reduce the oxidative damage of blue light and ultraviolet rays to eyes and eye skin; it can also promote eye microcirculation; tomato extract can prevent eye skin damage caused by solar radiation; it can reduce fibrin degradation and pigmentation, improve microcirculation, and promote collagen production, thereby reducing dark circles and fine lines around eyes; sea rice fermentation helps to improve eye fatigue and dark circles caused by poor sleep quality; the combination of the above ingredients can achieve synergistic effect, improve visual fatigue in multiple dimensions, relieve eye fatigue and dry eyes, and at the same time improve dark circles and fade eye fine lines.

[0046] Furthermore, in the above technical scheme, the schizophyllan contained in the fermentation product of Schizophyllan has a macromolecular polysaccharide spiral, and as a result, a gel-like substance is formed in an aqueous solution, which can evenly disperse and wrap each hydrophobic active ingredient, thereby increasing the sustained-release effect of the active ingredient. This composition is applied to oral beverages, and when the product is taken orally, its damage in the stomach can be reduced, and its absorption rate in the intestine can be increased; thereby further improving the eye care effect. In addition, this combination improves the stability of the overall product and reduces the use of suspensions in traditional liquid beverages. The schizophyllan contained in the fermentation product of Schizophyllan has a macromolecular polysaccharide spiral, and as a result, a gel-like substance is formed in an aqueous solution, and it can evenly disperse and wrap each hydrophobic active ingredient (such as tomato extract), and when the product is taken orally, its damage in the stomach can be reduced, and its absorption rate in the intestine can be increased.

[0047] In general, the product composition is different from conventional eye health products, not only in terms of the effect on improving eye health, but also in terms of ensuring product stability without the use of an external suspending agent.

[0048] Further optionally, in some embodiments of the present application, in parts by mass, including:

[0049] 3.2, 3.4, 3.5, 3.6, 3.9, 4.0, 4.1, 4.2, 4.3, 4.5, 4.6, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5 or the range between any two of the foregoing values ​​of Schizophyllum fermentation product;

[0050] Containing 2.1 parts, 2.2 parts, 2.5 parts, 2.6 parts, 2.7 parts, 2.8 parts, 2.9 parts, 3 parts, 3.3 parts, 3.4 parts, 3.5 parts, 3.6 parts, 3.9 parts, 4.0 parts, 4.1 parts, 4.2 parts, 4.3 parts, 4.4 parts, 4.5 parts of SOD yeast powder or the range between any two of the foregoing values;

[0051] 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2.0 parts, 2.1 parts, 2.2 parts, 2.3 parts, 2.4 parts, 2.5 parts, 2.8 parts, 2.9 parts, 3.0 parts of fermented sea rice, or a range between any two of the foregoing values;

[0052] 3.0 parts, 3.1 parts, 3.4 parts, 3.5 parts, 3.6 parts, 3.7 parts, 3.8 parts, 3.9 parts, 4.0 parts, 4.3 parts, 4.4 parts, 4.5 parts, 4.6 parts, 4.7 parts, 4.8 parts, 4.9 parts, 5.0 parts of mulberry fermented liquid or the range between any two of the foregoing values; and

[0053] 1.2 parts, 1.3 parts, 1.5 parts, 1.8 parts, 2 parts, 2.1 parts, 2.2 parts, 2.3 parts, 2.4 parts, 2.5 parts of tomato extract or the range between any two of the foregoing values.

[0054] Further optionally, in some embodiments of the present application, the fermentation-based composition for improving eye health includes, by weight: 3.5-5.0 parts of Schizophyllum fermentation product; 2.5-4.0 parts of SOD-containing yeast powder; 1.5-3.0 parts of sea rice fermentation product; 3.0-5.0 parts of mulberry fermentation liquid; and 1.2-2.5 parts of tomato extract.

[0055] In the above technical scheme, the fermented product of Schizophyllum: the fruiting body of Schizophyllum is a precious edible fungus, and the main active substance present in its fruiting body, mycelium and fermentation liquid is Schizophyllan. The fermented product of Schizophyllum obtained by liquid fermentation of Schizophyllum species has a high content of Schizophyllan and a clear color and appearance. Compared with the intracellular polysaccharide obtained by solid culture and extraction, it has stronger application feasibility and wider range.

[0056] After using the eyes for a long time, the eyes will experience dryness, blurred vision, soreness and swelling. Schizophyllum polysaccharide has high water retention and viscosity, which can regulate the water balance of the skin and maintain the cell structure. In addition, it can increase the number of long fiber cells and fill the gaps between skin cells. The above technical solution can not only enhance eye moisturizing, relieve eye fatigue and improve the moisture of the skin around the eyes by compounding Schizophyllum polysaccharide, but also enhance immunity and reduce the incidence of eye problems.

[0057] Furthermore, in the above technical scheme, by orally combining the fermented product of Schizophyllum with other ingredients, the stability of the product can be effectively improved, and it is beneficial to improve the sustained release effect of the active ingredients and improve the absorption effect. The Schizophyllum polysaccharide contained in the fermented product of Schizophyllum has a macromolecular polysaccharide spiral, which forms a gel-like substance in an aqueous solution, and can evenly disperse and wrap each active ingredient (tomato extract, SOD-containing yeast powder, sea rice fermented product, and each active ingredient of mulberry fermented liquid). When the product is taken orally, its destruction in the stomach can be reduced and its absorption rate in the intestine can be increased; thereby further improving the eye care effect.

[0058] Furthermore, in the above technical scheme, by compounding the fermented product of Schizophyllum with tomato extract, SOD-containing yeast powder, sea rice fermented product, and mulberry fermented liquid, and applying it to oral beverages, it is possible to ensure good stability of the beverage without using the suspension in traditional liquid beverages.

[0059] Furthermore, in the above technical scheme, sea rice fermentation: sea rice is a salt-alkali tolerant rice that grows on the beach. Sea rice fermentation is rich in various nutrients, including γ-aminobutyric acid and inositol hexaphosphate, etc., which can supplement the nutrients needed by the human body. At the same time, γ-aminobutyric acid can regulate the body's mood and sleep, and help improve eye fatigue and dark circles caused by poor sleep quality. In the above technical scheme, sea rice fermentation is compounded and taken orally with tomato extract, SOD-containing yeast powder, sea rice fermentation, and mulberry fermentation liquid, which can achieve a synergistic effect, improve visual fatigue, relieve eye fatigue and dry eyes, and can improve dark circles and dilute fine lines around the eyes, playing a multi-faceted role in protecting the eyes.

[0060] Furthermore, in the above technical solution, the yeast powder containing SOD: superoxide dismutase is an active substance derived from living organisms, and is a metal enzyme that can specifically remove superoxide anion free radicals; it can prevent cells from producing reactive oxygen and other oxides due to metabolism that damage the cell membrane system. In the above technical solution, the yeast powder containing SOD is compounded and taken orally with sea rice fermentation, tomato extract, sea rice fermentation, and mulberry fermentation liquid, which can achieve a synergistic effect, improve visual fatigue, relieve eye fatigue and dry eyes, and improve dark circles and lighten eye fine lines, playing a multi-faceted role in protecting the eyes.

[0061] Further optionally, in some embodiments of the present application, the above-mentioned SOD-containing yeast powder can be purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0062] Furthermore, in the above technical scheme, mulberry fermented liquid: mulberry is rich in anthocyanins, polysaccharides and polyphenols. The total phenolic, anthocyanin and flavonoid content in the mulberry fermented liquid prepared by the fermentation process is significantly improved. Studies have shown that anthocyanins can promote the regeneration of rhodopsin, increase visual sensitivity, and reduce the recovery time of retinal plaques, thereby protecting vision and eliminating visual fatigue. At the same time, it can reduce the oxidative damage of blue light and ultraviolet rays to the eyes and eye skin. It can also promote eye microcirculation. In the above technical scheme, the mulberry fermented liquid is compounded with SOD yeast powder, sea rice fermented product, tomato extract, and sea rice fermented product for oral administration, which can achieve a synergistic effect, improve visual fatigue, relieve eye fatigue and dry eyes, and can improve dark circles and dilute eye fine lines, playing a multi-faceted role in protecting the eyes.

[0063] Furthermore, in the above technical solution, tomato extract is rich in lycopene, and the polyunsaturated bond structure it contains has a strong antioxidant capacity, which is twice that of beta-carotene and 100 times that of vitamin E. Oral lycopene inhibits the upregulation of heme oxygenase, intercellular adhesion molecule and matrix metallopeptidase 1 mRNA induced by UVA1 and UVA / B; it can prevent eye skin damage caused by solar radiation. It can reduce fibrin degradation and pigment deposition, improve microcirculation, and promote collagen production, thereby reducing dark circles and fine lines around the eyes. In the above technical solution, mulberry fermentation liquid is compounded with SOD-containing yeast powder, sea rice fermentation, tomato extract, and sea rice fermentation, which can achieve a synergistic effect, improve visual fatigue, relieve eye fatigue and dry eyes, and improve dark circles and dilute eye fine lines, playing a multi-faceted role in protecting the eyes.

[0064] Furthermore, in some embodiments of the present application, preparing a Schizophyllum fermentation product comprises: based on the total volume of the culture medium, introducing 5% to 15% of the Schizophyllum seed solution into the culture medium, fermenting and culturing at 28°C to 30°C, 140r / min to 170r / min for 100h to 150h, and taking the supernatant.

[0065] Further optionally, in some embodiments of the present application, illustratively, preparing a Schizophyllum fermentation product comprises: based on the total volume of the culture medium, adding 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15% or a range between any two of the foregoing Schizophyllum seed liquid into the culture medium, fermenting and culturing for 100h, 110h, 120h, 130h, 140h, 150h or a range between any two of the foregoing conditions at 28°C, 29°C, 30°C or a temperature in the range between any two of the foregoing values, and 140r / min, 145r / min, 150r / min, 155r / min, 160r / min, 165r / min, 170r / min or a range between any two of the foregoing values, and taking the supernatant.

[0066] Exemplarily, in some embodiments of the present application, the preparation of the Schizophyllum fermentation product comprises: activating and culturing the Schizophyllum strain provided by the Guangdong Institute of Microbiology, wherein the culture contains yeast extract, magnesium sulfate, dihydrogen phosphate, glucose and the remainder of water. After activation, the Schizophyllum is obtained. 10% of the activated Schizophyllum (v / v, Schizophyllum seed liquid / total volume of the culture medium after accessing the seed liquid) is accessed into the culture medium, and the fermentation is terminated after culturing for 120 hours at 28°C-30°C and 140r / min-170r / min. The fermentation product is centrifuged to obtain the supernatant, which is then concentrated to obtain the Schizophyllum fermentation product, and the Schizophyllum fermentation broth is rich in Schizophyllum polysaccharide.

[0067] Further optionally, in some embodiments of the present application, the above-mentioned Schizophyllum (strain is from Guangdong Provincial Microbiological Culture Collection Center, numbered GDMCC 5.43).

[0068] Furthermore, in some embodiments of the present application, preparing yeast powder containing SOD includes: inoculating yeast strains into a culture medium for culturing; obtaining a fermentation product; and extracting and separating the fermentation product to obtain yeast powder containing SOD.

[0069] Illustratively, in some embodiments of the present application, SOD-containing yeast powder is obtained by fermentation, culture, extraction, separation and purification of yeast species, and is purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0070] Furthermore, in some embodiments of the present application, preparing the sea rice fermentation product includes: crushing the sea rice, adding water, fermenting with brewer's yeast at 25° C. to 30° C. for 40 h to 50 h, and obtaining the fermentation liquid.

[0071] Illustratively, in some embodiments of the present application, preparing sea rice fermentation includes: crushing the sea rice, adding water, fermenting with brewer's yeast at 25°C, 26°C, 27°C, 28°C, 29°C, 30°C or a range between any two of the foregoing values ​​for 40h, 41h, 42h, 43h, 44h, 45h, 46h, 47h, 48h, 49h, 50h or a range between any two of the foregoing values, and taking the fermentation liquid.

[0072] Further optionally, in some embodiments of the present application, illustratively, preparing the sea rice fermentation product includes: crushing the sea rice, adding a certain proportion of water, and fermenting with brewer's yeast at 28°C for 48 hours, then filtering and centrifuging to obtain the sea rice fermentation liquid, and concentrating and freeze-drying the fermentation liquid to obtain the sea rice fermentation product.

[0073] Furthermore, in some embodiments of the present application, preparing mulberry fermentation broth includes: based on the total mass of mulberry slurry, inoculating 0.01% to 0.04% lactic acid bacteria into the mulberry slurry, fermenting at 35° C. to 40° C. for 30 h to 48 h, and obtaining the fermentation broth.

[0074] Exemplarily, in some embodiments of the present application, preparing mulberry fermentation broth includes: based on the total mass of the mulberry slurry, inoculating the mulberry slurry with 0.01%, 0.02%, 0.03%, 0.04% or lactic acid bacteria in a range between any two of the foregoing values, and fermenting at 35°C, 36°C, 37°C, 38°C, 39°C, 40°C or in a range between any two of the foregoing values ​​for 30h, 31h, 32h, 33h, 34h, 35h, 36h, 37h, 38h, 40h, 42h, 43h, 45h, 46h, 48h or in a range between any two of the foregoing values ​​to obtain the fermentation broth.

[0075] Further optionally, in some embodiments of the present application, illustratively, preparing mulberry fermentation liquid includes: washing fresh mulberries, removing impurities and stems, placing them in a pulper for pulping, homogenizing, sterilizing, cold-cutting, inoculating 0.04% lactic acid bacteria, fermenting at a constant temperature of 37°C for 48 hours, then centrifuging, concentrating to a solid content of 55-65%, and sterilizing to obtain mulberry fermentation concentrated juice.

[0076] Furthermore, in some embodiments of the present application, preparing the tomato extract includes: mixing tomato pulp with pectinase and cellulase, treating at 40° C. to 60° C. for 2 h to 3 h, removing pectinase and cellulase, centrifuging, and taking a precipitate.

[0077] Illustratively, in some embodiments of the present application, preparing a tomato extract comprises: mixing tomato pulp with pectinase and cellulase, treating at 40°C, 41°C, 42°C, 43°C, 45°C, 48°C, 50°C, 52°C, 53°C, 54°C, 55°C, 56°C, 57°C, 58°C, 60°C or in a range between any two of the foregoing values ​​for 2 h, 2.2 h, 2.3 h, 2.5 h, 2.6 h, 2.7 h, 2.8 h, 2.9 h, 3 h or in a range between any two of the foregoing values, removing pectinase and cellulase, centrifuging, and taking a precipitate.

[0078] Further optionally, illustratively, in some embodiments of the present application, the preparation of tomato extract includes: washing the tomatoes and then scalding them with 100°C hot water to peel them, drying and crushing the tomato skins; beating and crushing the tomato flesh with a high-speed blender; mixing to obtain a slurry, adding 0.2-0.6% pectinase and cellulase (the sum of the two enzymes accounts for the mass percentage of the tomato slurry; the two enzymes are mixed in any proportion), treating at 50°C for 2-3h to remove 90% of non-pigment substances such as pectin and cellulose, and then centrifuging, washing the precipitate with 95% ethanol and filtering, separating the ethanol and vegetable oil phases, and freeze-drying the oil phase to obtain the tomato extract.

[0079] Some embodiments of the present application provide a beverage having the function of improving eye health, including: a fermentation-based composition for improving eye health provided in any of the aforementioned embodiments.

[0080] Further optionally, in some embodiments of the present application, the beverage does not contain a suspending agent.

[0081] The beverages mentioned above not only enhance the effect of the beverages in improving eye health by compounding the fermented products of Schizophyllum with other raw materials, but also greatly enhance the stability of the products. Compared with traditional beverages (which add a large amount of suspending agents), they are greener and more natural.

[0082] The beverage improves the stability of the overall product and reduces the use of suspensions in traditional liquid beverages. The schizophyllan contained in the fermented product of Schizophyllan has a macromolecular polysaccharide spiral that forms a gel-like substance in an aqueous solution, which can evenly disperse and encapsulate hydrophobic substances (such as tomato extract). When the product is taken orally, it can reduce its damage in the stomach and increase its absorption rate in the intestine.

[0083] In general, the above-mentioned beverages are different from conventional eye health products, not only in their effects on improving eye health, but also in ensuring product stability without the use of external suspending agents.

[0084] Further optionally, in some embodiments of the present application, the dosage form of the beverage includes: at least one of a beverage, soft candy or suckable jelly.

[0085] Some embodiments of the present application provide a method for preparing a beverage having the function of improving eye health, comprising:

[0086] Dissolving SOD-containing yeast powder and sea rice fermentation product to obtain a first mixed solution;

[0087] adding mulberry fermented liquid to the first mixed liquid to obtain a second mixed liquid;

[0088] dissolving the Schizophyllum fermented product to obtain a Schizophyllum fermented product dispersion; adding tomato extract to the Schizophyllum fermented product dispersion to obtain a third mixed solution;

[0089] The second mixed solution and the third mixed solution are mixed.

[0090] Further optionally, in some embodiments of the present application, illustratively, a method for preparing a beverage having the function of improving eye health comprises:

[0091] Step S1, weighing the formula amount of SOD yeast powder, sea rice fermentation product, and food sweetener, adding them into purified water and stirring them evenly at 35-45° C. until they are completely dissolved, then adding mulberry fermentation concentrated juice and stirring the mixture 1 evenly.

[0092] Step S2, add appropriate amount of water to the fermented product of Schizophyllum, stir and disperse it at 40-60°C to dissolve it (the concentration of the solution is 3-6 mg / ml), then add the tomato extract, and continue stirring for 8-15 minutes until it is evenly mixed to obtain mixed solution 2. Combine mixed solution 1 and mixed solution 2, stir them evenly, then add water to make up the volume.

[0093] Step S2: After preparation, the material is sterilized by ultra-high temperature instantaneous sterilization (UHT) technology and then filled into bottles.

[0094] The features and performance of the present application are further described in detail below in conjunction with the embodiments:

[0095] Examples and Comparative Examples

[0096] A fermentation-based composition for improving eye health is provided. The proportions of various embodiments and comparative examples are shown in Table 1, based on weight percentage.

[0097] Table 1

[0098]

[0099] Preparation process:

[0100] According to Table 1, weigh the formula amount of SOD yeast powder and sea rice fermentation product, add them into purified water, stir evenly at 35-45°C until completely dissolved, then add mulberry fermentation liquid, and stir evenly to form a mixed solution 1.

[0101] Add appropriate amount of water to the fermented product of Schizophyllum, stir and disperse under the condition of keeping warm at 40-60℃ (solution concentration is 3-6mg / ml), then add tomato extract, continue stirring for 8-15min until mixed evenly, and obtain mixed solution 2. Combine mixed solution 1 and mixed solution 2, stir evenly, then add, and add water to make up to volume.

[0102] After preparation, the material is sterilized using ultra-high temperature instantaneous sterilization (UHT) technology before being filled into bottles.

[0103] Performance Test:

[0104] 1. Antioxidant effect-ABTS free radical scavenging ability determination

[0105] Weigh ABTS and potassium persulfate and dissolve them in 10 mL of distilled water to prepare a 3.5 mmol ABTS solution. Place the solution at room temperature in a dark place for 16 h to obtain an ABTS solution, which is diluted with methanol to an application solution with an absorbance of 0.683 at 734 nm.

[0106] In a reaction container, add 0.2 mL of the sample of each embodiment and comparative example (vitamin C is used as a positive control group), 1.3 mL of ABTS free radical application solution, shake well, and stand at room temperature in the dark for 6 minutes, then measure the absorbance A1 of the solution at 734 nm. Use 95% methanol solution instead of ABTS free radical application solution as a control, and measure the absorbance A2 of the solution at 734 nm at room temperature. Use methanol solution instead of the sample to be tested as a control, and measure the absorbance A0 of the solution at 734 nm. Measure 3 times for each group, calculate the average value, and calculate the clearance rate of the sample according to the following formula. And draw a clearance rate and sample absorbance curve.

[0107] ABTS free radical scavenging rate (%) = (A0-A1-A2) / A0×100

[0108] Wherein: A0 refers to the absorbance value of the reaction system containing ABTS application solution but not the sample to be tested; A1 refers to the absorbance value after the sample reaction; A2 refers to the absorbance value of the reaction system containing the sample to be tested but not the ABTS application solution.

[0109] The test results are shown in the table below:

[0110] Table 2 Results of ABTS free radical scavenging ability and microcirculation improvement efficacy

[0111] Free radical scavenging rate% Blank Group 0 Positive control group 90 Comparative Example 1 43 Comparative Example 2 32 Comparative Example 3 40 Comparative Example 4 36 Example 1 76 Example 2 70 Example 3 78 Example 4 73

[0112] It can be seen from the data in the above table that the ABTS free radical scavenging ability of each embodiment is better than that of each comparative example; this shows that the various components of the composition of the present application achieve a synergistic effect, which can significantly improve the ABTS free radical scavenging ability and improve microcirculation efficacy; therefore, it can be used to relieve visual fatigue, improve dry eyes, and improve dark circles and fine lines around the eyes.

[0113] 2. Zebrafish test improves microcirculation

[0114] 110 4dpf wild-type AB strain zebrafish were randomly selected in a six-well plate, 30 in each well. The test solution was given in water solution in groups, and acetylsalicylic acid was used as the positive control. A normal control group and a model control group were set up at the same time, and the capacity of each well was 3mL. Except for the normal control group, the rest of the experimental groups were given ponatinib in water solution to induce a zebrafish microcirculatory disorder model. After the test sample was treated with acetylsalicylic acid for 18 hours, 10 zebrafish were randomly selected from each experimental group, and the blood flow velocity of zebrafish was measured using a heartbeat blood flow analysis system to evaluate the improvement effect of the test sample of each embodiment and comparative example on the blood flow velocity of zebrafish microcirculatory disorder.

[0115] The test results are shown in the table below:

[0116] Table 3

[0117] Intersegmental blood flow velocity (μm / s) Blank Group 496±28.2 Positive control group 681±28.05 Comparative Example 1 564±36.0 Comparative Example 2 572±40.2 Comparative Example 3 559±41.2 Comparative Example 4 568±38.2 Example 1 654±36.2 Example 2 646±50.0 Example 3 674±43.4 Example 4 665±32.0

[0118] 3. Crowd testing

[0119] The volunteers were selected to be between 25 and 50 years old, with easily fatigued eyesight and dark circles. Consume 1 stick a day for 28 days. Use VISIA to take facial images at 0D and 28D, and analyze the wrinkle scores with the system software. The higher the score, the more wrinkles there are. At the same time, the color change of dark circles around the eyes was detected. Eye color change value = D28 color value - D0 color value

[0120] A questionnaire survey was conducted using the visual fatigue symptom judgment criteria to evaluate the improvement of eye fatigue.

[0121] Table 4 Method for determining visual fatigue symptoms (semi-quantitative integration method)

[0122]

[0123] Note: “Occasionally” means 1-2 times / 2 days; “Sometimes” means 1-3 times / day; “Often” means >3 times / day

[0124] Judgment criteria: cured means there are points before taking the medicine, but no points after taking it; improved means there are points before and after taking it, but the points before taking it are greater than the points after taking it; ineffective means the points before and after taking it are the same or the points after taking it are greater than the points before taking it.

[0125] Then the improvement rate % = (cured number + improved number) / total number * 100%

[0126] After trying the product for 28 days, observe changes in dark circles and crow's feet around the eyes, and whether dry eyes are relieved.

[0127] The test results are shown in the table below:

[0128] Table 5 Results of alleviating visual fatigue, wrinkles around the eyes and chromaticity

[0129]

[0130]

[0131] It can be seen from the data in the above table that the eye fatigue improvement rate, fine line reduction effect, and chromaticity change value effect of each embodiment are better than those of the comparative examples; this can explain that the synergistic cooperation between the various components of the composition of the present application achieves a synergistic effect, effectively improving the eye fatigue improvement rate, fine line reduction effect, and chromaticity change value effect.

[0132] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A fermentation-based composition for improving eye health, characterized in that: In parts by mass, including:

2. The fermentation-based composition for improving eye health according to claim 1, characterized in that: In parts by mass, including:

3. The fermentation-based composition for improving eye health according to claim 1, characterized in that: The method for preparing the schizophyllum fermentation product comprises: adding 5% to 15% of schizophyllum seed liquid to the culture medium based on the total volume of the culture medium, fermenting and culturing for 100 to 150 hours at 28 to 30°C and 140 to 170 r / min, and taking the supernatant.

4. The fermentation-based composition for improving eye health according to claim 1, characterized in that: The preparation of the SOD-containing yeast powder comprises: inoculating yeast strains into a culture medium for culturing; obtaining a fermentation product; and extracting and separating the fermentation product to obtain the SOD-containing yeast powder.

5. The fermentation-based composition for improving eye health according to claim 1, characterized in that: The preparation of the sea rice fermented product comprises: crushing the sea rice, adding water, fermenting with brewer's yeast at 25° C. to 30° C. for 40 to 50 hours, and obtaining fermented liquid.

6. The fermentation-based composition for improving eye health according to claim 1, characterized in that: The preparation of the mulberry fermentation liquid comprises: based on the total mass of the mulberry slurry, inoculating 0.01% to 0.04% lactic acid bacteria into the mulberry slurry, fermenting at 35° C. to 40° C. for 30h to 48h, and obtaining the fermentation liquid.

7. The fermentation-based composition for improving eye health according to claim 1, characterized in that: The preparation of the tomato extract comprises: mixing tomato pulp with pectinase and cellulase, treating at 40-60°C for 2-3h, removing the pectinase and the cellulase, centrifuging, and taking a precipitate.

8. A beverage that improves eye health, characterized in that: include: The fermentation-based composition for improving eye health according to any one of claims 1 to 7.

9. The beverage for improving eye health according to claim 8, characterized in that: The beverage does not contain a suspension; Optionally, the beverage is in the form of at least one of a beverage, soft candy or suckable jelly.

10. The method for preparing a drink having the function of improving eye health according to any one of claims 8 to 9, characterized in that: include: Dissolving the SOD-containing yeast powder and the sea rice fermentation product to obtain a first mixed solution; adding mulberry fermented liquid to the first mixed liquid; obtaining a second mixed solution; dissolving the Schizophyllum fermented product to obtain a Schizophyllum fermented product dispersion; adding the tomato extract to the Schizophyllum fermentation product dispersion to obtain a third mixed solution; The second mixed liquid and the third mixed liquid are mixed.

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