88 results about "Loganin" patented technology

The invention provides a method for extracting cornel iridoid glycoside by virtue of ultrasonic-microwave synergetic extraction. The method comprises the following steps: (1) preparing cornus officinalis coarse powder; (2) adding the cornus officinalis coarse powder into a 80% methanol solution in a material-liquid ratio of (1:5)-(1:25), sealing, weighing and determining the weight, connecting a condensing device, and extracting for 3-15 minutes with microwave power of 300-700W and ultrasonic powder of 120-600W to obtain an extract; (3) carrying out suction filtration on the extract to obtain filtrate, keeping the constant volume, filtering, concentrating, drying and purifying to obtain the cornel iridoid glycoside. A method for determining the cornel iridoid glycoside comprises the following steps: a, preparing a mixed reference substance solution; b, preparing a test substance solution; c, determining the contents of morroniside, loganin, sweroside and cornel iridoid glycoside in the mixed reference substance and the test substance; d, respectively calculating the contents of the four glycosides according to the result of liquid chromatographic analysis.

The invention discloses a new medical application of loganin, and in particular relates to an application of the loganin in preparation of medicaments for treating diabetes, wherein the loganin is extracted from dogwood. The method for extracting the loganin comprises the following steps of: (1) performing water extraction and alcohol precipitation on dogwood coarse powder to obtain a dogwood water extraction and alcohol precipitation extracting solution; (2) loading the dogwood water extraction and alcohol precipitation extracting solution into a macroporous adsorption resin column, and performing enrichment to obtain dogwood iridoid glycosides; (3) performing chromatographic separation by using a silica gel column to obtain a loganin flow component; and (4) purifying to obtain a loganin monomeric compound. Pharmacodynamics experimental results prove that the loganin has the functions of reducing the content of blood sugar, serum AGEs and urokinase protein, raising the serum insulin level, relieving pathologic lesions of pancreas, kidney, liver, heart, testis and the like, and obviously improving a 'three more one less' symptom. Therefore, the loganin can be used for preparing the medicaments for treating the diabetes.

The invention provides a quality control method for extract concentrated liquor of condensed pills of six ingredients with rehmannia root, which comprises the following steps of: 1, measuring the specific gravity, paeonol content and loganin content of the extract concentrated liquor of the qualified condensed pills of six ingredients with rehmannia root, and collecting a near infrared spectrum; 2, establishing corresponding models between the near infrared spectrum and the specific gravity, the paeonol concentration and the loganin concentration in the extract concentrated liquor of the condensed pills of six ingredients with rehmannia root respectively by adopting chemometric software; 3, measuring the near infrared spectrum of an extract concentrated liquor sample, which is to be measured, of the condensed pills of six ingredients with rehmannia root; and 4, by utilizing the corresponding models, acquiring the specific gravity, the paeonol concentration and the loganin concentration of the extract concentrated liquor sample, which is to be measured, of the condensed pills of six ingredients with rehmannia root by the chemometric software respectively.

Liuwei Dihuang Decoction extract, comprising 11.4-17% by weight of polysaccharides, and/or 1.85-2.27% by weight of total glycosides (morroniside, loganin, paeoniflorin) and /or 23.5-35.5% by weight of mannotriose, wherein Liuwei Dihuang Decoction is composed of six traditional Chinese medicines of Rehmannia glutinosa, Cornus officinalis, Chinese yam, Alisma, Paeonol and Poria cocos in a weight ratio of 8:4:4:3:3:3.

The invention discloses a quality detection method of a ginseng basis-consolidating oral solution with the functions of nourishing yin and qi and consolidating basis. According to the quality detection method, the preparations of ginseng, dogwood, cortex moutan and rhizoma alismatis are distinguished with thin layer chromatography, and meanwhile the contents of ginsenosides Rg1 and Re of the ginseng, the content of paeonol of the cortex moutan, the contents of morroniside and loganin of the dogwood in the preparations are measured by adopting HPLC (high performance liquid chromatography). The quality detection method disclosed by the invention is stable, reliable, high in specificity and good in repeatability; content determination is carried out by selecting monarch drugs such as ginseng and cortex moutan containing volatile constituents in the prescription, so that the quality of the ginseng basis-consolidating oral solution can be comprehensively and effectively controlled, the stabilization for product quality is facilitated, the safety and effectiveness of clinical medication are guaranteed, and requirements of medical treatment and market are preferably met.

The invention discloses a novel method for simultaneously measuring contents of multiple active ingredients of dogwood. The method is used for simultaneously measuring the contents of seven ingredients, namely loganin, gallic acid, 5-HMF (hydroxymethyl furfural), protocatechuic acid, morroniside, sweroside and cor-nuside, in dogwood medicinal materials from four main producing areas by utilizing a high performance liquid diode array detection method. The method comprises the steps of: carrying out gradient elution at the flow speed of 1.0 mL.min-1 by utilizing a Phenoemenex C18 polar chromatographic column and taking acetonitrile and 0.1% methanoic acid solution as mobile phases, wherein the column temperature is 30 DEG C, the detection wavelengths are 220 nm, 240 nm, 260 nm, 265 nm and 280 nm. The contents of the loganin, the gallic acid, the 5-HMF, the protocatechuic acid, the morroniside, the sweroside and the cor-nuside in each sample are respectively 0.22-0.800 mg/g, 0.002-0.024 mg/g, 0.069-3.775 mg/g, 8.192-28.111 mg/g, 0.048-0.598 mg/g, 1.228-9.311 mg/g and 1.450-8.381 mg/g. The method is simple and accurate, is good in reproducibility and is capable of providing bases for comprehensively evaluating and controlling the quality of dogwood decoction pieces.

The invention especially relates to a method for extraction and content determination of loganin, belonging to the technical field of determination methods. The method provided by the invention has the advantages of simple operation and accurate determination. According to a technical scheme in the invention, the method comprises the following steps: weighing loganin and adding methanol to prepare a loganin solution with a concentration of 60 mu g/ml; weighing to-be-determined powder, sieving the powder with a sieve No. 3, placing the sieved powder into a cone flask with a plug, adding 80% methanol, carrying out ultrasonic treatment for 30 min, then carrying out centrifugation for 20 min, taking a supernatant and subjecting the supernatant to microporous membrane filtration; taking a loganin reference solution and carrying out sample introduction 3 to 10 times, wherein a peak area RSD is 0.84%; weighing medicinal powder and calculating loganin content, wherein RSD of the medicinal powder is 1.07%; and weighing 6 parts of the medicinal powder with known content, putting the medicinal powder into a round bottom flask, adding the loganin reference solution and carrying out determination.

The invention provides a multi-index fast detection method of dogwood medicinal materials. The multi-index fast detection method comprises the following steps of: acquiring the dogwood medicinal materials; determining the contents of morroniside and loganin in the dogwood by using a high-performance liquid chromatography, determining the content of water by using a drying method, and determining the content of extract by using a cold-extraction method; collecting near-infrared spectrum data of dogwood medicinal material powder; preprocessing spectrum at the modeling waveband of the near-infrared spectrum; establishing a fast detection model for the water, the extract and the morroniside and the loganin in the dogwood medicinal materials; and using the established model for fast determination of the water, the extract and the morroniside and the loganin in the dogwood medicinal materials. The multi-index fast detection method provided by the invention has the advantages that a near-infrared spectrum technology is introduced as the fast detection method of the contents of the water, the extract, the morroniside and the loganin in the dogwood medicinal materials; and compared with the traditional method, the established analysis method has the advantages that whether the quality of the medicinal materials is qualified can be fast judged, whether the medicinal materials can enter the following production-process links can be determined, the requirements of fastness and high efficiency in production can be met, and the application prospect for site medicinal-material screening and comprehensive quality evaluation is achieved.

The invention discloses a method for extraction and purification of four iridoid glycosides in cornus officinalis. The method comprises the following steps of 1, accurately weighing cornus officinalis, carrying out heating reflux extraction by an ethanol aqueous solution having a volume concentration of 40-90% for 1-4h, wherein the weight of the ethanol aqueous solution is 5-15 times the weight of the weighed cornus officinalis, carrying out filtration, carrying out extraction 2-4 times, and merging the filtrate, and 2, carrying out purification of the merged filtrate by a mixed macroporous resin comprising HPD300, LSA30 and SP825, carrying out elution by 1-4BV of an ethanol aqueous solution having a volume concentration of 20-80% at an elution flow rate of 1-3BV/h, collecting the eluent, and carrying out condensation to obtain a mixture of four iridoid glycosides. The method has the advantages that 1, processes are simple and safe; a process is short; a cost is low; and industrial production is convenient; and 2, through extraction and purification by the mixed resins, high-purity loganin, swertiamarin, morroniside and cornuside are obtained.

The invention discloses a method for quickly detecting six ingredients of dogwood by use of UFLC (Ultra Fast Liquid Chromatography). The method comprises the following steps: at first, preparing a test solution; then preparing six reference solutions of gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, sweroside and cornuside; adopting the UFLC for detection, and performing gradient elution: 0-3 min, 2-5% A; 3-5 min, 5-17% A; 5-10 min 17-17.5%; 10-11 min, 17.5-30% A; 11-15 min, 30-60% A; finally, calculating the result according to setting of a standard curve. The method has the advantages of being simple, quick and high in sensitivity, precision and accuracy.

The invention discloses a quality control method of a traditional Chinese medicine compound preparation for treating osteoporosis. The quality control method comprises the following steps: (1) by taking eucommia ulmoides as a contrast medicinal material and taking pinoresinol diglucoside and geniposidic acid as comparison products, carrying out qualitative identification on eucommia ulmoides in the traditional Chinese medicine compound preparation by adopting a thin-layer chromatography; (2) by taking radix achyranthis bidentatae as a contrast medicinal material and taking beta-ecdysterone as a comparison product, carrying out qualitative identification on radix achyranthis bidentatae in the traditional Chinese medicine compound preparation by adopting the thin-layer chromatography; (3) by taking wolfberry fruits as contrast medicinal materials, carrying out qualitative identification on the wolfberry fruits in the traditional Chinese medicine compound preparation by adopting the thin-layer chromatography; and (4) determining the content of three active components (or index components) including epimedium glucoside, gentiopicroside and loganin acid in the traditional Chinese medicine compound preparation by adopting a high performance liquid chromatography. According to the quality control method, the quality of the traditional Chinese medicine compound preparation can be controlled very well, and the stability of a product production process is monitored; and the quality of the traditional Chinese medicine compound preparation is stable, uniform and controllable.

The invention relates to a method for processing dogwood with wine. The method comprises the following steps: 1, taking clean dogwood meat medicinal material, and adding the dogwood meat into yellow wine or white wine according to the mass ratio of the yellow wine or the white wine and the clean dogwood meat medicinal material of 0.15-0.25; 2, braising a mixture obtained in the step 1 for 30-90 minutes, wherein when the content of loganin tested in the step 1 is low, the braising time is reduced, or when the content of oleanolic acid tested in the step 1 is too high, the braising time is extended; 3, heating, boosting and steaming a product obtained in the step 2 in an autociaving pot, wherein when the steaming time reaches 110-125 DEG C, taking out the product is taken out after steaming for 20-60 minutes, wherein when the content of the loganin or morroniside tested in the step 2 is low, the steaming time is reduced, or wherein when the content of the loganin or morroniside tested in the step 2 is low, the steaming temperature is raised; 4, drying a product obtained in the step 3 for 2-3 hours in a 80-DEG C in a drying oven and taking out; and 5, airing, and hermetically packaging.

The invention provides a content measuring method for four components in a Chinese medicinal composition. The Chinese medicinal composition consists of Chinese medicaments such as ginseng, dwarf lilyturf tuber, dogwood, root of red rooted salvia, sauted spina data seed, Chinese taxillus twig, red peony root, ground beetle, nardostachys root and the like. The Chinese medicinal composition is clinically used for treating arrhythmia. The content of paeoniflorin, content of loganin, content of berberine and content of salvianolic acid B in the Chinese medicinal composition are measured at the same time by adopting an efficient liquid phase method; and the method is simple, convenient and feasible, the measurement results are accurate and reliable, and the method can be used for evaluating the quality of the Chinese medicinal composition.

The invention relates to a method for extracting high-purity loganin from cornus officinalis fruits. The method includes: using traditional Chinese medicine cornus officinalis fruits as the raw material, and performing reflux extraction, acid-reaction impurity removing, organic-solvent purification, crystallization, recrystallization and the like to obtain the loganin with the purity being larger than 95%. The method has the advantages that acid reaction is used to remove impurities which affects the crystallization, and the method is simple in process, low in production cost and suitable for large-scale industrial production.

The invention relates to a method for simultaneously preparing high-purity morroniside and high-purity loganin from dogwood extract. The method comprises the following steps: performing macroporous adsorption resin enrichment and normal phase silica gel preliminary separation on the dogwood extract, then performing treatment by a medium-pressure reverse phase silica gel chromatographic column, performing gradient elution by taking ethanol and water as a mobile phase, mixing the same components, and performing concentration under reduced pressure to respectively obtain a morroniside crude product of which the purity is greater than or equal to 90% and a loganin crude product of which the purity is greater than or equal to 90%; and respectively performing purification with a Sephadex LH-20 gel column, performing elution by adopting a chloroform and methanol mixed solvent, mixing the same components, and performing evaporation to dryness under reduced pressure to obtain the high-purity morroniside and the high-purity loganin. The method is simple and easy in operation and mild in conditions and is suitable for simultaneously preparing and producing morroniside of which the purity is greater than or equal to 98% and loganin of which the purity is greater than or equal to 98% in a large scale from the dogwood extract.

The invention discloses a UPLC fingerprint spectrum detection method of lamiophlomis rotata (Benth.)Kudo and preparations thereof. The method includes the following steps of: (a) the preparation of areference substance solution; (b) the preparation of a test solution; (c) the provision of UPLC detection conditions; (d) the formation of a standard fingerprint spectrum with sesamoside, caffeic acid, chlorogenic acid, shanzhiside methyl ester, loganin, 8-O-acetyl shanzhiside methyl ester, forsythiaside B, galuteolin, verbascoside, Isoacteoside and the like adopted as reference peaks; and (e) thequality control of the fingerprint spectrum. According to the method, during test solution preparation, an extraction solvent is environment-friendly. The method is simple. Compared with a traditional HPLC method, the method is more convenient and rapider, and save energy and reduce energy consumption. The separation effect of the characteristic peaks of the fingerprint spectrum of the lamiophlomis rotata (Benth.)Kudo and the preparation thereof is good; and characteristic compositions and medicinal compositions can be reserved completely. The method has the advantages of good repeatability,high stability, high precision and strong specificity. The method can accurately and quickly control the lamiophlomis rotata (Benth.)Kudo and the preparations thereof.

The invention relates to a rehmannia decoction pharmaceutical composition and a preparation method thereof, and a detection method. The pharmaceutical composition consists of the following formula: 0.5-1.5 parts of prepared rehmannia roots, 0.5-1.5 parts of Morinda pulp, 0.5-1.5 parts of cornus officinalis pulp, 0.5-1.5 parts of dendrobium, 0.5-1.5 parts of wine-processed cistanche, 0.5-1.5 partsof prepared common monkshood branched roots, 0.5-1.5 parts of Chinese magnoliavine fruits, 0.5-1.5 parts of cinnamon, 0.5-1.5 parts of poria cocos, 0.5-1.5 parts of dwarf lilyturf tuber, 0.5-1.5 partsof rhizoma acori graminei, 0.5-1.5 parts of milkwort root, 4-6 parts of raw ginger, 3-6 parts of Chinese dates and 0.5-1.5 parts of mint. The composition is prepared by a method including water extraction, freeze drying and the like. The process solves the technical problems that in the prior art, morroniside, loganin and schisandrin are lost due to reduced-pressure drying, and the dry paste powder is granular and hard. The invention determines a specific detection method for qualitative identification, fingerprint spectrum and content determination. The good detection method effectively reflects the advantages and disadvantages of the material reference mass and solves the technical problem that quality detection methods in the prior art are difficult to reflect the advantages and disadvantages of the material reference mass.

The invention discloses a fingerprint spectrum establishing method for controlling the processing process parameters of wine-soaked cornus officinalis Sieb.et Zucc. According to the method, the processing process parameters, namely, the content (peak area) of loganin, morroniside, gallic aci, protocatechuic acid, and 5-hydroxymethyl furfural, in a fingerprint spectrum in a processing process are controlled, so that the processing technology of the wine-soaked cornus officinalis Sieb.et Zucc. can be controlled. The influence of each of the three factors of processing time, covered moistening time and steaming time on the five chemical components before and after the processing is investigated, so that optimal processing time, covered moistening time and steaming time can be confirmed.

The invention provides a method for simultaneously determining the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid. The method comprises the following steps: extracting a to-be-tested sample by virtue of a polarity organic solvent under an ultrasonic condition, so as to obtain an extracting solution; and carrying out high performance liquid chromatography on the obtained extracting solution to obtain the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid in the to-be-tested sample according to standard curves of the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid and a liquid chromatogram of the to-be-tested sample. By utilizing the method provided by the invention, the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid can be simultaneously determined; and furthermore, the separation degree is good, the sensitivity and the simultaneously are high, the recovery rate is relatively high, and an effective detection measure is provided for a preparation utilizing the five active components as quality test indexes.

The invention discloses a method for extracting and separating a compound loganin from flowers and flower buds or branches and leaves of a featured herbal medicine Loliseria hypoglauca Miq in Guangxi province. The method is characterized by comprising the steps of extracting through high-concentration low-grade alcohols (such as methanol and ethanol), concentrating and then extracting through ethyl acetate to remove liposoluble components, extracting with n-butyl alcohol, concentrating through polyamide column chromatography, performing SephadexLH-20 gel chromatography, eluting with low-grade alcohols (such as methanol and ethanol) as an eluent, recovering the solvent for concentrating, separating, crystallizing, and re-crystallizing to obtain loganin with the purity of more than 95%. According to the method, the natural medicine active ingredient having wide market prospects can be recovered from flowers and clipped waste branches and leaves after deflowering of Loliseria hypoglauca Miq, and the method has a tremendous promotion effect on the comprehensive utilization of Loliseria hypoglauca Miq.