Quality detection method of ginseng basis-consolidating oral solution

A technology of oral liquid and ginseng, which is applied in the field of quality inspection of ginseng guben oral liquid, can solve the problems of not being able to fully control the quality of ginseng guben oral liquid, not enough to represent ten medicinal materials, poor accuracy and controllability, etc. Achieve scientific and reasonable detection methods, meet medical and market needs, and ensure stability

Pending Publication Date: 2017-02-01
杨本官 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this standard only qualitatively or quantitatively analyzes the two flavors of ginseng and paeonol in the preparation, which is narrow in coverage and not enough to represent the ten medicinal materials in the preparation. The content of paeonol is determined by ultraviolet spectrophotometry, which is cumbersome to operate. Poor accuracy and controllability
Another bibliographical report measures the content of ginsenoside Rg1 in ginseng Guben oral liquid by TLC scanning method (Xu Guihua et al. Deter...

Method used

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  • Quality detection method of ginseng basis-consolidating oral solution

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Effect test

Embodiment 1

[0050] The HPLC methodology research of embodiment 1 ginsenoside Rg1, ginsenoside Re

[0051] 1) Instruments and reagents: Agilent 1100 high performance liquid chromatograph, VWD detector; acetonitrile and methanol are chromatographically pure, other reagents are analytically pure, and water is double distilled water.

[0052] 2) Chromatographic conditions: Kromasil C 18 Chromatographic column (4.6mm×150mm, 5μm); mobile phase is acetonitrile-water (19:81); detection wavelength is 203nm; flow rate is 1mL / min, column temperature is 25°C.

[0053] 3) Preparation of the test solution: Accurately measure 50mL of this product, concentrate to 25mL, let it cool, put it in a separatory funnel, add ethyl acetate to shake and extract twice (30mL, 20mL), discard the ethyl acetate layer , the water layer was shaken and extracted 4 times with water-saturated n-butanol, 20mL each time, combined the n-butanol extracts, washed 3 times with 5% sodium hydroxide solution, 30mL each time, discard...

Embodiment 2

[0088] The HPLC methodology research of embodiment 2 paeonol

[0089] 1) Instruments and reagents: SHMADZU high-performance liquid chromatography, SPD-10A ultraviolet detector. Methanol is chromatographically pure, other reagents are analytically pure, and water is double distilled water.

[0090]2) Chromatographic conditions: HYPER SIL BDS C 18 5μm column; the mobile phase is methanol: water = 60:40; the detection wavelength is 274nm; the flow rate is 0.8mL / min.

[0091] 3) Preparation of the test solution: draw 3mL of this product, put it in a 25mL volumetric flask, add absolute ethanol to dilute to the mark, shake well, and centrifuge. Take the supernatant and filter it with a 0.45 μm microporous membrane to obtain the obtained solution.

[0092] 4) Preparation of reference substance solution: Accurately weigh an appropriate amount of paeonol reference substance (Paeonol reference substance batch number: 110708-200506, China Institute for the Control of Pharmaceutical an...

Embodiment 3

[0128] The HPLC methodology research of embodiment 3 morroniside, loganin

[0129] 1) Instruments and reagents: Agilent 1100 high performance liquid chromatography, DAD detector. Acetonitrile and methanol are chromatographically pure, other reagents are analytically pure, and water is double distilled water.

[0130] 2) Chromatographic conditions: Kromasil C 18 Chromatographic column (4.6mm×250mm, 5μm); mobile phase: use acetonitrile as mobile phase A, 0.3% phosphoric acid solution as mobile phase B, carry out gradient elution as specified in the table below;

[0131]

[0132] The detection wavelength is 240nm; the column temperature is 35°C; the flow rate is 1mL / min.

[0133] 3) Preparation of the test solution: Accurately measure 5mL of this product under the difference in filling volume, put it in a 50mL measuring bottle, add 50% methanol to dilute to the mark, shake well, centrifuge, take the supernatant and use a microporous filter membrane (0.45μm) filter, that is....

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Abstract

The invention discloses a quality detection method of a ginseng basis-consolidating oral solution with the functions of nourishing yin and qi and consolidating basis. According to the quality detection method, the preparations of ginseng, dogwood, cortex moutan and rhizoma alismatis are distinguished with thin layer chromatography, and meanwhile the contents of ginsenosides Rg1 and Re of the ginseng, the content of paeonol of the cortex moutan, the contents of morroniside and loganin of the dogwood in the preparations are measured by adopting HPLC (high performance liquid chromatography). The quality detection method disclosed by the invention is stable, reliable, high in specificity and good in repeatability; content determination is carried out by selecting monarch drugs such as ginseng and cortex moutan containing volatile constituents in the prescription, so that the quality of the ginseng basis-consolidating oral solution can be comprehensively and effectively controlled, the stabilization for product quality is facilitated, the safety and effectiveness of clinical medication are guaranteed, and requirements of medical treatment and market are preferably met.

Description

technical field [0001] The invention relates to a quality detection method of ginseng-guben oral liquid with the functions of nourishing yin and replenishing qi, and strengthening the base and nourishing the essence, and belongs to the field of analysis of traditional Chinese medicine preparations. Background technique [0002] Guben Guben Pills is a classic prescription, which is included in the first volume of "Drug Standards of the Ministry of Health of the People's Republic of China Chinese Medicine Prescriptions". This prescription is based on Liuwei Dihuang Pills, adding ginseng, asparagus, Radix Ophiopogon japonicus and raw rehmannia glutinosa. Composed of 10 traditional Chinese medicines, it has the functions of nourishing yin and replenishing qi, consolidating the foundation and cultivating vitality, and has a good curative effect on the syndromes of yin deficiency and qi deficiency. It is mainly used for postoperative rehabilitation in clinical practice. [0003] ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/90G01N30/94
CPCG01N30/02G01N30/06G01N30/90G01N30/94
Inventor 张则平张贵民苏瑞强刘武占
Owner 杨本官
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