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46results about How to "The detection method is scientific and reasonable" patented technology

Polarity finder of electronic device of automatic braiding machine and testing method thereof

The invention belongs to an electronic device polarity supervisory instrument and testing method of automatic braider. It characterized in that several vacuum suction nozzles are equispaced in circumference of swash plate, and divided into two groups to realize the shifting that a component is transferred from one station to the next station. The detecting station, component rotating station, quadratic detecting station of the component and component abandoning station are set symmetrically under the vacuum suction nozzles to realize the polarity detecting, polarity adjusting, quadratic polarity detecting and the abandoning of components in course of shifting that a component is transferred from one station to the next station. The whole apparatus is simple and compact, can realize synchronously the detecting in course of shifting of components, the efficiency of work is improved and the cost is decreased. The apparatus has the function of self-centering.
Owner:TIANJIN UNIV OF SCI & TECH

Method for detecting overload crane by detecting power

InactiveCN101691189AScientific and reliable design principlePracticalSafety gearPower factorMathematical model
The invention relates to a method for detecting the overload of a crane by detecting the power, comprising the following steps: (1) detecting the working voltage and the working current of an electric motor; (2) calculating the load of the crane according to the following mathematic model: m=KEtaUICOSPhi / gv, wherein the m is the load of the crane, the K is the coefficient, the U is the working voltage of the electric motor, the I is working current of the electric motor, the COSPhi is the power factor of the electric motor, the Eta is the craning efficiency of the crane, v is the ascending speed of the load of the crane and g is the gravity acceleration of the load; and (3) comparing the calculated load of the crane with the rated load of the crane and judging the overload of the crane. The method is used for detecting the overload of the crane by detecting the working voltage and the working current of the electric motor according to the ascending speed of the load of the crane and related parameters provided by the user without using a weight sensor and a standard weight to calibrate, can not be affected by the fluctuation of the voltage and has the characteristics of rapid response speed, high degree of accuracy, wide application range and the like.
Owner:TIANJIN TIANAN CRANE ELECTRIC EQUIP

Transmission line icing detection method and system based on image processing

The invention discloses a transmission line icing detection method and system based on image processing, belonging to the technical field of transmission line maintenance. The method includes collecting an original image; preprocessing the original image to get the target image; extracting the edge of the iced transmission line in the target image to obtain the iced transmission line contour; theice-covered transmission line profile is divided into several sections, and the average diameter of the ice-covered transmission line is obtained by the ice-covered transmission line profile diameterof each section. According to the average diameter of the iced transmission line and the actual diameter of the transmission line, the thickness of the iced transmission line is obtained and output. The invention also discloses a system, a device and a storage medium for realizing the method. The technical proposal of the invention is used for detecting the icing thickness of the transmission line, has high safety and accurate result, and greatly reduces the cost compared with the manual patrol inspection or the unmanned aerial vehicle patrol inspection adopted at present.
Owner:ANHUI JIYUAN SOFTWARE CO LTD +1

Detection method for traditional Chinese medicine preparation compounded Sichuan fritillary bulb extract tablets

The invention discloses a detection method for traditional Chinese medicine preparation compounded Sichuan fritillary bulb extract tablets. The detection method comprises the steps of carrying out thin-layer chromatography qualitative identification and carrying out content determination, wherein thin-layer chromatography qualitative identification comprises the steps of identifying ephedrannin and neoephedrine A in the preparation, identifying polygala root and cynanchum atratum and identifying glycyrrhiza uralensis and schisandra fruit; content determination comprises the step of determining the content of ephedrine hydrochloride in the preparation. The detection method disclosed by the invention has the advantages that the mixing of radix ephedrae and cynanchum atratum is effectively controlled, and meanwhile, the composition and content of the compounded Sichuan fritillary bulb extract tablets is effectively controlled, so that the drug can be safer and more effective.
Owner:九寨沟天然药业股份有限公司

Detection method of medicinal preparation for treating arthralgia

The invention discloses a detection method of a medicinal preparation for treating arthralgia. The detection method is composed of character presentation, identification, inspection and content measurement, wherein the identification is to identify gaultheria yunnanensis, sargentgloryvine stem, psammosilene tunicoides, and Alangium lamarchii Thwaites, and the content measurement is to measure the content of the Alangium lamarchii Thwaites alkali in capsules according to Chinese pharmacopeia 2010 edition Part one appendix VID high performance liquid chromatorgraphy, and to measure the content of the total flavone in the capsules according to Chinese pharmacopeia 2010 edition Part one appendix VA UV-VIS spectrophotometry. The invention provides the detection method of Jingulian capsule preparations for treating painful joints. The employed method is scientific and reasonable, has high accuracy and good reproducibility, can comprehensively and effectively control the quality of the Jingulian capsules, and better ensures the clinic curative effect and safety of the preparation.
Owner:GUIZHOU YIBAI PHARMA CO LTD

Quality detection device for door/window screen and using method thereof

ActiveCN104655444AEasy to set upThe test results are close to the realStructural/machines measurementDrive motorEngineering
The invention discloses a quality detection device for a door / window screen, belonging to the field of quality detection. The quality detection device disclosed by the invention comprises an overall frame, a screen clamping mechanism, a detection mechanism and a loading mechanism, wherein the screen clamping mechanism adjusts the relative height of a screen fixing rod on the overall frame by utilizing a tensioning mechanism; a screen fixing clamping groove is formed in the overall frame, and the upper and lower sides of a to-be-detected screen are clamped by virtue of the screen fixing clamping groove and the screen fixing rod; the detection mechanism comprises a driving motor, a lead screw sliding table, a support frame, a detection meter bracket, a digital indicator and a sliding rail; the lead screw sliding table is driven by the driving motor and is used for driving the support frame to move along the horizontal direction; a measuring head of the digital indicator faces the to-be-detected screen, and the digital indicator is positioned in the middle of the to-be-detected screen; and the loading mechanism hooks the to-be-detected screen by virtue of a hook so as to realize tension loading of the to-be-detected screen. The special detection device and method are designed according to the screen detection standard, the structure is simple, the detection is convenient, and the intelligent degree is high.
Owner:常州常工院技术转移有限公司

Fuel on-line detection method of gasoline and diesel oil mixing flexible fuel engine

The invention relates to a fuel on-line detection method of a gasoline and diesel oil mixing flexible fuel engine. The fuel on-line detection method is characterized in that power equipment is used, and the power equipment comprises an engine electronic control unit, a flexible fuel compression ignition engine, a piezoresistive cylinder pressure sensor and a fuel recognition electronic control unit; the fuel recognition electronic control unit comprises a circulating fuel-injection quantity calculating module, an accumulative heat release calculating module, a fuel volume calorific value calculating module and a fuel recognition module; the engine electronic control unit collects the fuel temperature, the rail pressure and the injection time, and the piezoresistive cylinder pressure sensor collects cylinder pressure and sends the cylinder pressure to the fuel recognition electronic control unit; the circulating fuel-injection quantity calculating module calculates the circulating fuel-injection quantity and sends the circulating fuel-injection quantity to the fuel volume calorific value calculating module; the accumulative heat release calculating module calculates the circulating accumulative heat release and sends the circulating accumulative heat release the fuel volume calorific value calculating module; the fuel volume calorific value calculating module calculates the volume calorific value of mixing fuel and sends the volume calorific value of the mixing fuel to the fuel recognition module; the fuel recognition module calculates the mixing ratio and feeds back the mixing ratio to the engine electronic control unit to perform closed-loop control. The fuel on-line detection method can be widely applied to the on-line detection of engine fuel.
Owner:TSINGHUA UNIV

Method for detecting wind-cold cold granules

The invention discloses a method for detecting wind-cold cold granules. The method comprises thin layer identification of Chinese ephedra and platycodon root and content determination of ephedrine hydrochloride, wherein identification of the Chinese ephedra is performed by taking ephedrine hydrochloride as a reference substance and a mixed solution including ethyl acetate, methanol and water as a developing agent and using a thin layer chromatography; and the identification of the platycodon root is performed by taking a platycodon root contrast medicinal material as a reference substance and a mixed solution including n-hexane, ethyl acetate and glacial acetic acid as a developing agent and using the thin layer chromatography. By adopting the method, the quality of the main components including the Chinese ephedra and the platycodon root, which cause problems easily, in a prescription is effectively controlled, so that the quality of the wind-cold cold granules is better and more comprehensively reflected, fake and shoddy wind-cold cold granules produced by illegal manufacturers can be effectively detected and identified, and then the safety and effectiveness of mass medication are ensured.
Owner:SICHUAN FENGCHUN PHARMA

Detection method for nucleic acid amplification product

The invention provides a detection method for a nucleic acid amplification product, particularly a detection and pollution control method for the nucleic acid amplification reaction product with no need for opening a reaction vessel. The detection method includes steps that: before a nucleic acid amplification reaction, solid-state microcrystalline waxes wrapped with nucleic acid dye are added into the reaction vessel, and the microcrystalline waxes are guaranteed not to contact with reaction liquid; during the nucleic acid amplification reaction, the amplification reaction temperature is maintained to be lower than the melting point of the microcrystalline waxes, and the microcrystalline wax beads are guaranteed not to contact with the reaction liquid; after the nucleic acid amplification reaction, the reaction vessel is heated to the melting point of the microcrystalline waxes which are converted into a liquid state from a solid state and float on the upper layer of the nucleic acid reaction liquid; the wrapped dye enters the reaction liquid, is evenly mixed with the nucleic acid amplification reaction liquid, the nucleic acid amplification product is combined with the dye, and a color reaction is performed.
Owner:陶志勇 +2

Quality detection method for dysuria remedying granules

The invention discloses a quality detection method for Chinese patent medicine dysuria remedying granules for treating chronic prostatitis. The quality detection method comprises the steps that the thin layer chromatography is adopted for recognizing cinnamon, Chinese violets, radix scrophulariae, golden cypress, radix sophorae flavescentis, rhizoma dioscoreae hypoglaucae and radix cyathulae in the preparation, and meanwhile HPLC is adopted for determining the content of berberine hydrochloride of the golden cypress in the preparation and the content of amygdalin in peach kernels. The quality detection method is stable, reliable, high in specificity, good in reproducibility, capable of comprehensively and effectively controlling the quality of the dysuria remedying granules, and capable of stabilizing the product quality, ensuring the clinical medication safety and effectiveness and better meeting the requirement for medical treatment and market.
Owner:LUNAN HOPE PHARM CO LTD

Detection method for compound Dan Yin paste

The invention provides a detection method for compound Dan Yin paste. The method includes identification on characters and components, inspection, and content measurement. The invention aims to incomplete detection standards of the compound Dan Yin paste in the prior art, in which there are no any quality detection measures besides detections on common indices according to preparation general rules, so that the detection methods, in the prior art, has defects of monitoring medicine quality. In the method, the following items are added: identification between capillary artemisia and artemisia apiacea, identification between salvia miltiorrhiza and gentiana macrophylla, identification on radix bupleuri and licorice roots, content measurement of tanshinone IIA, and content measurement of salvianolic acid B. The method can effectively detect whether the corresponding medicine raw materials are less than standard contents or are not added due to lawless enterprises or not, and also can detect adulteration of the artemisia apiacea and gentiana macrophylla, which are liable to be confused, thereby preventing mis-use. The method can effectively guarantee the quality of the compound Dan Yin paste, ensures that the compound Dan Yin paste to be safe and effective, can guarantees clinical curative effects of the medicine and protects patient benefits.
Owner:SICHUAN FENGCHUN PHARMA

Method for detecting performance of air purifying product on killing bacteriophage in air

The invention discloses a method for detecting the performance of an air purifying product on killing bacteriophage in the air. The method comprises following steps: step one, preparing a fresh bacteriophage stock solution, measuring the titer of the stock solution, and diluting the stock solution; step two, setting same conditions of a test cabin and a blank cabin, and placing an purifying product in the test cabin; step three, releasing the diluted bacteriophage solution in an aerosol mode, stirring 2 to 5 minutes, and then allowing the system to stand still for 2 to 5 minutes; step four, collecting the primary samples in the test cabin and blank cabin, then starting the purifying product in the test cabin, and after a while, collecting the residue samples in the test cabin and the blankcabin; and step five, culturing, calculating the natural attenuation rate, and evaluating the purifying effect. The provided method can detect multiple bacteriophage host bacteria, and has a wide application range. Furthermore, natural attenuation rate is taken into account, the objectiveness and realness of the detection results are ensured, and the method can be used to accurately evaluate thevirus killing effect of an air purifying product.
Owner:广州市微生物研究所 +1

Detection method of high-quality Sihuang dysentery-stopping granules

The present invention discloses a detection method of high-quality Sihuang dysentery-stopping granules. According to the quality detection method, the coptis chinensis, golden cypress, rheum officinale, scutellaria baicalensis, isatis roots and liquorice in a preparation are identified through thin-layer chromatography, and meanwhile the content of baicalin in the preparation is determined throughan HPLC. After a quality process is changed, the content of baicalin is remarkably increased, and in combination with the detection method provided by the invention, the detection result is stable and reliable, the specificity is strong, the reproducibility is good, the quality of the Sihuang dysentery-stopping granules can be comprehensively and effectively controlled, the product quality in themarket can be stabilized, and the veterinary drug safety and effectiveness can be ensured, so that the market requirements can be better met.
Owner:山东琪康生物技术有限公司

Concrete pole rebar quality tester and detection method by using tester

The invention relates to a concrete pole rebar quality tester and a detection method by using the tester. The tester is composed of a fixed stand, an exciter, a rotary encoder, a cell box, a signal preprocessor, an ARM (analog digital conversion) processor, a magnet measuring element and the like, wherein the fixed stand can be sheathed on a concrete pole to be tested, and the magnet measuring element and the exciter are installed together. When the concrete pole rebar quality tester is used for detection, the fixed stand is tightly sheathed on the concrete pole to be tested; the clip of the cell box is clamped on the belt of an inspector; the exciter magnetizes the rebar to generate back magnetic flux; the magnet measuring element measures the back magnetic flux passing through an armature; and the back magnetic flux is relevant to the diameter, the amount, the position, the corrosion and the like of the rebar, i.e. the back magnetic flux is converted into an electrical signal, is amplified, filtered and the like by the signal preprocessor, is subjected to analog digital conversion by the ARM processor and is analyzed to obtain a parameter which needs to be detected. The tester disclosed by the invention is simple to manufacture and is convenient to operate, the rebar in the concrete pole can be detected, the diameter, the position, the amount and the corrosion degree of the rebar can be accurately judged, and the detection method is scientific and reasonable.
Owner:陈厚桂

Method for detecting trichloropropanol in corn oil by utilizing gas chromatograph-mass spectrometer

The invention belongs to the technical field of oil detection and in particular relates to a method for detecting trichloropropanol in corn oil by utilizing a gas chromatograph-mass spectrometer. Thedetection method comprises the following steps: (1) determining instrument detection conditions; (2) preparing reagents and materials; (3) performing sample pretreatment; (4) establishing a marked line; and (5) calculating the trichloropropanol content. According to the method for detecting trichloropropanol in corn oil by utilizing the gas chromatograph-mass spectrometer disclosed by the invention, the detection sensitivity, accuracy and stability can be obviously improved, and the detection method is simple, rapid, stable and reliable. Meanwhile, the complicated process of existing nationalstandards at home at present is overcome, and the problem that an international technique for detecting the trichloropropanol in corn oil does not exist at present is solved. The method disclosed by the invention can be widely applied to determination of trichloropropanol in vegetable oil, is capable of realizing batch detection, and brings convenience to the quality control of trichloropropanol content in each vegetable oil production unit.
Owner:SHANDONG SANXING CORN IND SCI CO LTD

Detection method of speaker in telephone conference based on NDN

InactiveCN103167191AWorkaround for blind speaker detection bugReduce useless traffic transmissionSpecial service for subscribersSubstation remote connection/disconnectionTeleconferenceTraffic volume
The invention relates to a method of detecting a speaker in an ACT telephone conference. The method includes a step 101), a step 102) and a step 103). The step 101) is that in the ACT telephone conference, an audience list is maintained at a conference participant end. The conference participant is informed that a speaking node is arranged as the emerging information of a new speaker by using a broadcast way when the new speaker emerges in conference participants. The step 102) is that the notice is replied by using the maintained audience list after the audience receives the information. Specific information of the new speaker node is inquired by a request packet delivered by an audience node. The step 103) is that the request packet delivered by all audiences is replied by the stored speaker list by the new speaker node. The conference participants comprise all the audiences and all the speakers. According to the detection method of the speaker in the telephone conference based on NDN, the audience is informed passively that the new speaker emerges, thus the expenditure of a listen-in user in the telephone conference is saved. Useless flow transferring of a network is reduced. A network bandwidth load is relieved. The network transferring efficiency is effectively improved.
Owner:INST OF ACOUSTICS CHINESE ACAD OF SCI

Method for evaluating sleep improvement effect of dairy product by zebra fish

The invention provides a method for evaluating the sleep improvement effect of a dairy product by using zebra fish. Influence factors such as dairy product treatment time selection, dairy product treatment concentration selection and instrument parameter selection are investigated, a zebra fish model capable of evaluating the sleep improvement effect of the dairy product is successfully constructed, the detection accuracy is high, the detection method is scientific and reasonable, the modeling period is short, so that the detection cost is greatly reduced, and the practical feasibility is provided for popularizing and popularizing the detection method to broad masses of people, enterprises and governments.
Owner:HANGZHOU HUANTE BIOLOGICAL TECH CO LTD

Detection method and detector for multiple color ratio high efficiency false proof luminescent material capable of loading cipher

This invention discloses a method and apparatus for measuring counterfeit deterrence material with multiple colour ratios and code loaded. And it comprises a high-density intelligence detecting element with emission, conversion and receiving and is combined with output and magnification processing system of digital signals to synchronously collectively detect, convert and process different areas of the whole object to be measured and let different areas form a set of colour ratio parameters separately. And it solves the problems of outdated measuring method, thin wave range, complicated system and being unable to accomplish multiple codes in the technique field of printing counterfeit deterrence. It is widely used in the counterfeit deterrence of current printing bills, bond papers, badges, marked commodity and of stamp pad used in seal, ink pad, signature and inkstone.
Owner:北京友邦联合高新技术有限公司

Detection method of compound Danyin ointment

The invention provides a detection method for compound Dan Yin paste. The method includes identification on characters and components, inspection, and content measurement. The invention aims to incomplete detection standards of the compound Dan Yin paste in the prior art, in which there are no any quality detection measures besides detections on common indices according to preparation general rules, so that the detection methods, in the prior art, has defects of monitoring medicine quality. In the method, the following items are added: identification between capillary artemisia and artemisia apiacea, identification between salvia miltiorrhiza and gentiana macrophylla, identification on radix bupleuri and licorice roots, content measurement of tanshinone IIA, and content measurement of salvianolic acid B. The method can effectively detect whether the corresponding medicine raw materials are less than standard contents or are not added due to lawless enterprises or not, and also can detect adulteration of the artemisia apiacea and gentiana macrophylla, which are liable to be confused, thereby preventing mis-use. The method can effectively guarantee the quality of the compound Dan Yin paste, ensures that the compound Dan Yin paste to be safe and effective, can guarantees clinical curative effects of the medicine and protects patient benefits.
Owner:SICHUAN FENGCHUN PHARMA

Primer group and probe for building specific quantitative PCR (polymerase chain reaction) precise detection by transgenic maize MON88017 and method thereof

The invention provides a primer group and probe for building specific quantitative PCR (polymerase chain reaction) precise detection by transgenic maize MON88017 and a detecting method thereof. The sequence of a forward primer is 5'-GGGCTTAGATGAGAAACTTCACGAT-3'; the sequence of a reverse primer is 5'-CCGACTCTCTTCTCAAGCATATGA-3'; the sequence of the probe is 5'-FAM CGCGCCAAAGCTTACTCGAGGTCA-TAMARA-3'. The primer group and the probe provided by the invention have the advantages that the specificity is good; the specific detection of the transgenic maize MON88017 can be realized; the detection method has the advantages of high amplification efficiency, high accuracy degree and good sensitivity degree. The problem that no high-sensitivity quantitative PCR precise detection technology by the transgenic maize MON88017 exists in the prior art can be effectively solved. The method can be applied to detection of the transgenic maize MON88017 and products of the transgenic maize MON88017.
Owner:四川省农业科学院分析测试中心

A quality detection device for door and window gauze and its application method

ActiveCN104655444BEasy to set upThe test results are close to the realStructural/machines measurementDrive motorEngineering
The invention discloses a quality detection device for a door / window screen, belonging to the field of quality detection. The quality detection device disclosed by the invention comprises an overall frame, a screen clamping mechanism, a detection mechanism and a loading mechanism, wherein the screen clamping mechanism adjusts the relative height of a screen fixing rod on the overall frame by utilizing a tensioning mechanism; a screen fixing clamping groove is formed in the overall frame, and the upper and lower sides of a to-be-detected screen are clamped by virtue of the screen fixing clamping groove and the screen fixing rod; the detection mechanism comprises a driving motor, a lead screw sliding table, a support frame, a detection meter bracket, a digital indicator and a sliding rail; the lead screw sliding table is driven by the driving motor and is used for driving the support frame to move along the horizontal direction; a measuring head of the digital indicator faces the to-be-detected screen, and the digital indicator is positioned in the middle of the to-be-detected screen; and the loading mechanism hooks the to-be-detected screen by virtue of a hook so as to realize tension loading of the to-be-detected screen. The special detection device and method are designed according to the screen detection standard, the structure is simple, the detection is convenient, and the intelligent degree is high.
Owner:常州常工院技术转移有限公司

A method for evaluating the safety of meat products using zebrafish

ActiveCN111766354BAdd processing stepsThe real environment of the evaluation resultsPreparing sample for investigationTesting foodBiotechnologyAnimal science
The invention provides a method for evaluating the safety of meat products by using zebrafish. Firstly, the meat product is treated with bionic digestion and extraction to obtain the meat sample extract, and then the processing time of the meat sample extract is determined to be 24 hours. The treatment concentration was 1.4-5.6 mg / mL to construct a zebrafish model and evaluate the safety of meat products. The zebrafish model constructed by the present invention and the method for evaluating the safety of meat products have high detection accuracy, the detection method is scientific and reasonable, and the short modeling cycle greatly reduces the detection cost, which provides a basis for popularizing and popularizing the detection method to the general public, enterprises and governments. Realistic feasibility.
Owner:HANGZHOU HUANTE BIOLOGICAL TECH CO LTD +1

Concrete pole rebar quality tester and detection method by using tester

The invention relates to a concrete pole rebar quality tester and a detection method by using the tester. The tester is composed of a fixed stand, an exciter, a rotary encoder, a cell box, a signal preprocessor, an ARM (analog digital conversion) processor, a magnet measuring element and the like, wherein the fixed stand can be sheathed on a concrete pole to be tested, and the magnet measuring element and the exciter are installed together. When the concrete pole rebar quality tester is used for detection, the fixed stand is tightly sheathed on the concrete pole to be tested; the clip of the cell box is clamped on the belt of an inspector; the exciter magnetizes the rebar to generate back magnetic flux; the magnet measuring element measures the back magnetic flux passing through an armature; and the back magnetic flux is relevant to the diameter, the amount, the position, the corrosion and the like of the rebar, i.e. the back magnetic flux is converted into an electrical signal, is amplified, filtered and the like by the signal preprocessor, is subjected to analog digital conversion by the ARM processor and is analyzed to obtain a parameter which needs to be detected. The tester disclosed by the invention is simple to manufacture and is convenient to operate, the rebar in the concrete pole can be detected, the diameter, the position, the amount and the corrosion degree of the rebar can be accurately judged, and the detection method is scientific and reasonable.
Owner:陈厚桂

Slab Inclusion Detection Method

The invention provides a method for detecting inclusions in a slab, which belongs to the technical field of continuous casting. The method comprises selecting a fixed-length slab to be inspected for inclusions as the slab to be inspected, and performing surface quenching on the slab to be inspected before grain boundary embrittlement. Cool; cut the slab to be tested into a slab cross-section of a set thickness along the drawing direction; perform low-power pickling on the cross-section of the slab cross-section; observe the quenched area of ​​the slab cross-section to see if cracks occur; If cracks are formed in the quenching area of ​​the transverse sample of the slab, it is determined that there are large inclusions in the slab to be detected; the present invention realizes continuous analysis and detection of the inclusions on the entire fixed-length slab, thereby improving the accuracy of the detection results. The technical effect of improving the performance and reducing the cost of detection.
Owner:CONTINUOUS CASTING TECH ENG OF CHINA

Transgenic maize mon88017 constructs a primer set and probe for accurate detection of specific quantitative PCR and its method

The invention provides a primer group and probe for building specific quantitative PCR (polymerase chain reaction) precise detection by transgenic maize MON88017 and a detecting method thereof. The sequence of a forward primer is 5'-GGGCTTAGATGAGAAACTTCACGAT-3'; the sequence of a reverse primer is 5'-CCGACTCTCTTCTCAAGCATATGA-3'; the sequence of the probe is 5'-FAM CGCGCCAAAGCTTACTCGAGGTCA-TAMARA-3'. The primer group and the probe provided by the invention have the advantages that the specificity is good; the specific detection of the transgenic maize MON88017 can be realized; the detection method has the advantages of high amplification efficiency, high accuracy degree and good sensitivity degree. The problem that no high-sensitivity quantitative PCR precise detection technology by the transgenic maize MON88017 exists in the prior art can be effectively solved. The method can be applied to detection of the transgenic maize MON88017 and products of the transgenic maize MON88017.
Owner:四川省农业科学院分析测试中心

Method for evaluating safety of vegetables and fruits by using zebra fish

The invention provides a method for evaluating the safety of vegetables and fruits by using zebra fish. The method comprises the following steps of constructing a zebra fish toxicity evaluation model, pre-treating the vegetables and the fruits in a manner of enzymolysis first and then chemical extraction to obtain an extract of a detection product, treating the extract by using the zebra fish to obtain the toxicity evaluation data, and analyzing and evaluating the safety of the vegetables and the fruits. The method provided by the invention is high in detection accuracy, scientific and reasonable in detection method and short in modeling period, so that the detection cost is greatly reduced, and practical feasibility is provided for popularizing and popularizing the detection method to the broad public, enterprises and governments.
Owner:南京新环检测科技有限公司
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