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A method for evaluating the safety of meat products using zebrafish

A technology for meat products and zebrafish, which is applied in the preparation of test samples, material inspection products, and test food, etc. It can solve the problems of unknown physical and chemical properties, difficult detection, and chemical hazards, and achieve scientific and reasonable detection methods. Effects of cost reduction and high detection accuracy

Active Publication Date: 2022-04-26
HANGZHOU HUANTE BIOLOGICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current food safety inspection items are basically physical and chemical inspections and biochemical inspections. The detection indicators are mechanized and poorly related to organisms, and have defects that cannot be ignored: 1) Due to the interaction between various chemicals, the residues may also be low Chemicals that are lower than the standard value are harmful to the human body, so whether the residual amount of harmful substances below the standard value is absolutely safe for the human body, there is uncertainty
2) The existing detection system only detects known harmful substances listed in the testing standards, and cannot detect harmful substances that are not listed in the testing standards or unknown. We cannot judge and prevent possible harm caused by food poisoning unknown substances
[0007] 1) Unable to detect the synergistic toxicity of different chemical substances to the human body
Due to the unknown physical and chemical properties of different chemicals, there is uncertainty about whether they can produce synergistic effect or synergistic reduction when interacting; it is more difficult to judge the synergistic toxicity of the sample to be tested than the toxicity of a single toxic substance
[0008] 2) Unknown additives cannot be detected, only known chemical substances can be detected
If there are analogues, derivatives of known chemical substances and their metabolites in the human body as unknown additives, it is difficult to detect
[0009] 3) There are many types of toxic substances, considering cost factors such as time, manpower, and material resources, it is impossible to cover everything and achieve full inspection
[0013] To sum up, how to provide a suitable method to evaluate the safety of meat products with zebrafish, provide safety guarantee for people to eat meat products with confidence, provide quality control guarantee for meat products produced by suppliers with confidence, and reassure regulatory agencies Supervising the safety of meat products in the production and distribution links and providing technical support is a technical problem that has not been solved by those skilled in the art; it is also a major technical challenge to establish testing standards for meat products using biological means

Method used

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  • A method for evaluating the safety of meat products using zebrafish
  • A method for evaluating the safety of meat products using zebrafish
  • A method for evaluating the safety of meat products using zebrafish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 determines the content volume ratio of meat products and acetonitrile in the pretreatment stage

[0043] 1. Purpose of the experiment

[0044] In this experiment, only the content volume ratio of meat products and acetonitrile was changed, and the rest of the experimental conditions were unchanged, to explore the effect of different content volume ratios of meat products and acetonitrile (set as 1:1, 1:1.5 and 1:2) on the dissolution The effect of oxygen to determine the optimal content volume ratio of meat products and acetonitrile.

[0045] 2. Experimental equipment

[0046] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China).

[0047] 3. Experimental method

[0048] Take commercially available antibiotic-free meat ("antibiotic-free meat" refers to animal source food that does not use any chemical drugs, antibiotics, and synthetic hormones in the breeding process, and is purely naturally farmed animal source food), and...

Embodiment 2

[0053] Embodiment 2 determines the processing time of the meat sample extract

[0054] 1. Purpose of the experiment

[0055] In this experiment, only the processing time of the meat sample extract was changed, and the rest of the experimental conditions were kept unchanged to explore the effects of different processing times (set at 6h, 24h and 48h) on the mortality of zebrafish and the detection of water quality, so as to determine the best The processing time of the meat sample extract.

[0056] 2. Experimental equipment

[0057] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China).

[0058] 3. Experimental method

[0059] 1) Pretreatment

[0060] Take meat products and use a homogenizer to break them evenly. After homogenizing for 30 seconds, take 5g of homogenized meat and add 10ml of prepared simulated gastric juice (containing sodium chloride 5.18g / 1000ml, potassium chloride 0.49g / 1000ml, pepsin 3g / 1000ml), after vortexing for 30s, a...

Embodiment 3

[0081] Embodiment 3 determines the processing concentration of the meat sample extract

[0082] 1. Purpose of the experiment

[0083] In this experiment, on the basis of the treatment time of 24 hours, only the treatment concentration of the meat sample extract was changed, and the rest of the experimental conditions were unchanged to explore different treatment concentrations (set to 0.7mg / mL, 1.4mg / mL, 2.8mg / mL, 5.6mg / mL and 11.2mg / mL) on the mortality of zebrafish in order to determine the optimal concentration of meat sample extract.

[0084] 2. Experimental equipment

[0085] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China).

[0086] 3. Experimental method

[0087] 1) Pretreatment

[0088] Take meat products and use a homogenizer to break them evenly. After homogenizing for 30 seconds, take 5g of homogenized meat and add 10ml of prepared simulated gastric juice (containing sodium chloride 5.18g / 1000ml, potassium chloride 0.49g / 1000m...

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Abstract

The invention provides a method for evaluating the safety of meat products by using zebrafish. Firstly, the meat product is treated with bionic digestion and extraction to obtain the meat sample extract, and then the processing time of the meat sample extract is determined to be 24 hours. The treatment concentration was 1.4-5.6 mg / mL to construct a zebrafish model and evaluate the safety of meat products. The zebrafish model constructed by the present invention and the method for evaluating the safety of meat products have high detection accuracy, the detection method is scientific and reasonable, and the short modeling cycle greatly reduces the detection cost, which provides a basis for popularizing and popularizing the detection method to the general public, enterprises and governments. Realistic feasibility.

Description

technical field [0001] The invention relates to the technical field of safety evaluation of meat products, in particular to a method for evaluating the safety of meat products by using zebrafish. Background technique [0002] Meat products are an indispensable consumer food in people's daily life, such as chicken, duck, fish, pig, cattle, sheep and other animal meat products, most of which come from farming. With the increasing demand of residents for daily products, driven by economic interests, the abuse of veterinary drugs (including antibiotics and their semi-synthetic products, chemically synthesized drugs and other additives) in the breeding industry has also intensified, resulting in the use of veterinary drugs in animal-derived foods. The residual content exceeds the standard; some unscrupulous farmers even use various adulteration methods to make "synthetic meat" to make huge profits. The risk of eating is higher. All kinds of strange phenomena have led to increas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/12G01N1/28
CPCG01N33/12G01N1/28
Inventor 戴明珠俞航萍陆夏逸李红群沈锡明李春启
Owner HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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