A method for evaluating the safety of meat products using zebrafish
A technology for meat products and zebrafish, which is applied in the preparation of test samples, material inspection products, and test food, etc. It can solve the problems of unknown physical and chemical properties, difficult detection, and chemical hazards, and achieve scientific and reasonable detection methods. Effects of cost reduction and high detection accuracy
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Embodiment 1
[0042] Embodiment 1 determines the content volume ratio of meat products and acetonitrile in the pretreatment stage
[0043] 1. Purpose of the experiment
[0044] In this experiment, only the content volume ratio of meat products and acetonitrile was changed, and the rest of the experimental conditions were unchanged, to explore the effect of different content volume ratios of meat products and acetonitrile (set as 1:1, 1:1.5 and 1:2) on the dissolution The effect of oxygen to determine the optimal content volume ratio of meat products and acetonitrile.
[0045] 2. Experimental equipment
[0046] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China).
[0047] 3. Experimental method
[0048] Take commercially available antibiotic-free meat ("antibiotic-free meat" refers to animal source food that does not use any chemical drugs, antibiotics, and synthetic hormones in the breeding process, and is purely naturally farmed animal source food), and...
Embodiment 2
[0053] Embodiment 2 determines the processing time of the meat sample extract
[0054] 1. Purpose of the experiment
[0055] In this experiment, only the processing time of the meat sample extract was changed, and the rest of the experimental conditions were kept unchanged to explore the effects of different processing times (set at 6h, 24h and 48h) on the mortality of zebrafish and the detection of water quality, so as to determine the best The processing time of the meat sample extract.
[0056] 2. Experimental equipment
[0057] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China).
[0058] 3. Experimental method
[0059] 1) Pretreatment
[0060] Take meat products and use a homogenizer to break them evenly. After homogenizing for 30 seconds, take 5g of homogenized meat and add 10ml of prepared simulated gastric juice (containing sodium chloride 5.18g / 1000ml, potassium chloride 0.49g / 1000ml, pepsin 3g / 1000ml), after vortexing for 30s, a...
Embodiment 3
[0081] Embodiment 3 determines the processing concentration of the meat sample extract
[0082] 1. Purpose of the experiment
[0083] In this experiment, on the basis of the treatment time of 24 hours, only the treatment concentration of the meat sample extract was changed, and the rest of the experimental conditions were unchanged to explore different treatment concentrations (set to 0.7mg / mL, 1.4mg / mL, 2.8mg / mL, 5.6mg / mL and 11.2mg / mL) on the mortality of zebrafish in order to determine the optimal concentration of meat sample extract.
[0084] 2. Experimental equipment
[0085] Dissecting microscope (SZX7, OLYMPUS, Japan); 6-well plate (Fisher Scientific, China).
[0086] 3. Experimental method
[0087] 1) Pretreatment
[0088] Take meat products and use a homogenizer to break them evenly. After homogenizing for 30 seconds, take 5g of homogenized meat and add 10ml of prepared simulated gastric juice (containing sodium chloride 5.18g / 1000ml, potassium chloride 0.49g / 1000m...
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