45results about How to "Short modeling cycle" patented technology

The invention discloses a calculation method for obtaining an antenna arm vibration abatement response. According to the antenna arm vibration abatement scheme of a piezoelectric material, the stress of the piezoelectric material is equivalent to an internal moment, and the strain of the piezoelectric material is equivalent to a displacement difference to obtain an analytic coupling calculation model of satellite dynamics, vibration abatement and gesture control. Through the resolving of the model, the antenna vibration frequency response and a time response after vibration abatement can be obtained, the layout analysis of the piezoelectric material on the antenna arm is realized, and the active vibration abatement affect analysis of the antenna arm is realized. An obtained calculation model is a coupling system model which contains the satellite dynamics, the vibration abatement and the gesture control on a top layer, and the cross coupling of each analysis link is fully considered to finish response calculation and also realize the analysis of a vibration transfer mechanism. The stress of the piezoelectric material is equivalent to the internal moment, the strain of the piezoelectric material is equivalent to the displacement difference, and therefore, the method has the advantages of being simple, practical, short in modeling period and convenient in engineering application.

The invention belongs to a power device system in the technical field of aviation, and relates to a method for correcting the installation thrust of an engine model. The method comprises the following steps: (1.1) acquiring an engine bench thrust Fi data table; (1.2) constructing an engine bench thrust model Ft by adopting a three-dimensional interpolation algorithm; (1.3) carrying out an air inlet blowing test; (1.4) modeling by using an interpolation method; (1.5) establishing an air inlet loss correction model K1; (1.6) building a power extraction loss coefficient database; (1.7) establishing a power extraction loss correction model K2; (1.8) building a spray pipe afterbody resistance loss coefficient database; (1.9) establishing a spray pipe afterbody resistance loss correction model K3; (1.10) finishing modelling of an engine installation thrust model. The method has the advantages of high calculation accuracy, simulation instantaneity, easiness in implementation, short modeling period and high engineering applicability.

An ice coating simulation method for a 220kV transmission line tower-coupling system is provided. The method comprises the following steps: (1) performing finite element modeling of an ice-covered transmission line tower-coupling system, including: modeling a tower by using a unit mirror command, modeling of an insulator and a split conductor spacer, and modeling of split sub-conductors and groundlines; (2) performing form-finding on the transmission line tower-coupling system; (3) calculating the ice-covered loads of the transmission line tower-coupling system; (4) calculating the ice-covered-wind loads of the transmission line tower-coupling system; and (5) performing response calculation and data extraction analysis under the ice coating. According to the method provided by the presentinvention, finite element modeling of the refined tower line is performed by using the unit mirror command, the modeling period is short, and the calculation efficiency is high; according to the actual arrangement manner of the split conductor, the coupling effect between the split sub-conductors is considered, and the model is more refined; and the analytical method and the finite element methodare combined to simulate the conductor, and the technical problems of finite element modeling and form-finding of the overhead line are solved.

The invention provides an establishment method of an endoplasmic reticulum stress induced mouse acute liver injury model. The establishment method orderly comprises the following steps: carrying out intragastric administration treatment on mice by use of an endoplasmic reticulum stress inducer with a dosage of 0.2ml / 10g for each mouse, and completing the establishment of the mouse acute liver injury model in 8-48 hours after administration, wherein the endoplasmic reticulum stress inducer is prepared by a method comprising the steps of taking (0.5-4)mg of tunicamycin for dissolving 100 microliters of dimethyl sulfoxide to obtain a preservation solution, taking 100 microliters of preservation solution and adding 19.9ml of double distilled water to 20ml, and thus completing the preparation of the endoplasmic reticulum stress inducer. The mouse model of the induced acute liver injury is established by inducing the endoplasmic reticulum stress effect; through the study on the mechanism of action of the endoplasmic reticulum stress, the self regulation ability of cells in the stress state can be further known, and the occurrence mechanism of the liver diseases can be further known; therefore, new intervention and treatment measures can be adopted for the liver diseases to achieve the purposes of preventing and treating diseases.

The invention discloses a construction method of animal models of cognitive decline caused by anxiety combined with continuous sleep loss as well as application of the animal models. The constructionmethod of the animal models of cognitive decline caused by anxiety combined with continuous sleep loss comprises the following steps: stimulating experimental animals by performing combined noise intervention, electrical stimulation intervention and light interference intervention; and then, performing animal behavior tests on the experimental animals after completion of the interventions. The construction method of the animal models of cognitive decline caused by anxiety combined with continuous sleep loss disclosed by the invention focuses cognitive decline synchronously caused by anxiety combined with continuous sleep loss; and moreover, the construction method is easy to operate, short in modeling cycle, good in modeling effects, and non-invasive to the animals. The animal models obtained by the construction method can be closer to scenarios of health symptoms of target populations with cognitive decline caused by the status of anxiety and continuous sleep loss due to high pressure, heavy workload and emotional tension; and thus, the animal models can be used for effective evaluation on lifestyle, diet and drugs on symptoms of cognitive decline caused by anxiety combined with continuous sleep loss. Moreover, reliable evaluation models are also provided for screening of related foods and health products, drug research and development, and the like.

The invention relates to an evaluation method for therapeutic effect of CAR-T immune cells and a device thereof. Specifically, the method comprises the steps of: 1) preparation of CAR-T cell: constructing a CAR-T plasmid and packaging to lentivirus; obtaining a T cell of the subject, amplifying the cell after activation; and infecting the activated T cell with lentivirus, obtaining the CAR-T cellexpressing an antibody of the target gene; 2) preparation of 3D tumor-like organs: obtaining tumor tissue cells of the same subject as in step 1), obtaining primary cells, mixing primary cells with matrigel, and introducing matrigel and fluorocarbon oil containing primary cells into a three-way device respectively, and obtaining an organ-like sphere and culturing the material; 3) co-culturing the3D tumor-like organs with the CAR-T cells obtained in the step 1); and 4) measuring the survival rate of the tumor cells in the tumor-like organ spheres, and obtaining the therapeutic effect of the CAR-T cells. The method of the invention is simple, the preparation is fast, and the degree of reduction is high.

The invention discloses a numerical simulation method of heat exchange between an environment and an air conditioner unit outdoor machine. The method comprises the steps that firstly an outdoor machine three-dimensional model is built according to an installation design scheme of the air conditioner unit outdoor machine, then the outdoor machine three-dimensional model is solved, and finally the optimum installation position of the air conditioner unit outdoor machine is determined according to calculation results. According to the numerical simulation method, a CFD simulation software is adopted, a mode that figures, cartoons and detailed digits are combined is used for expressing actual results, and the method is more persuasive; due to the fact that the modeling period is short, the modeling process is easy and convenient, a large amount of time is saved, software simulation is close to the temperature air flow distribution in actual conditions, and the method has high reliability; through software simulation, the method assists and is matched with a data center air conditioner design to the utmost extent, application effects are expected, and a reasonable and scientific solution is provided.

The invention discloses a unilateral ureteral ligation recanalisation animal model and a building method thereof. The method comprises the following steps of: 1) preparing an elastic plastic tube; and 2) after implanting an elastic plastic fat pad into the left ureter of an SD male mouse to pressurize to perform ligation obstruction, performing a surgery 7 days later for the second time, in the surgery, taking out the elastic plastic tube, dredging the left ureter to release the left kidney obstruction and separate the envelope of the left kidney at the same time, and ligaturing the right ureter with a NO. 4 thread. In addition, the invention also discloses the animal model built by the method. The animal model can reduce renal functions and cause renal tubule mesenchyme fibrosis change. The model building period is short; the response is quick; the cost is low; the model is convenient to use; the performance of the model is stable; the repeatability is good; pathomorphological changes of kidney after obstruction can be observed; the functional level of the kidney can be studied; and the relation between the pathomorphological changes and the functional level can be embodied; and the animal model provides a new study target for clinical research and development of medicaments for preventing and treating renal fibrosis.

The invention relates to an endometriosis fibrosis animal model construction method and application thereof and belongs to the technical field of animal models. According to a preparation method of anendometriosis fibrosis animal model, estrogen is subcutaneously injected to neck and back of an SPF-grade BALB / c female mouse and endometrial tissue fragments are intraperitoneally injected, so thatthe endometriosis fibrosis animal model is constructed. The preparation method has the advantages that the model has high conformity with clinical pathologic characteristics of endometriosis; the model is convenient to operate, the modeling period is short and the modeling success rate is high; an efficient modeling method is provided for researches of the endometriosis. Meanwhile, the model can be used as an ideal model for exploring the pathogenesis and an effective diagnosis and treatment strategy of endometriosis fibrosis.

The invention discloses a model for inducing drug resistance in a pseudomonas aeruginosa animal as well as a construction method and an application of the model. With the application of the model constructed by the method, better simulation of a phenomenon of PA infection in respiratory tract of a patient who accepts wide-spectrum antibiotics clinically for a long time can be achieved, and development and change rule of the drug resistance of pseudomonas aeruginosa in a mouse body can be observed, so as to simulate the change of the bacterium (the pseudomonas aeruginosa) in the body and to facilitate study on antibacterial drug-resistance medicine treatment; and meanwhile, the animal model, as a lung index evaluating animal model, is more intuitive, accurate in evaluating result, short in construction cycle and high in efficiency.

The invention discloses a method for establishing a skin-sensitized food allergy animal model, and belongs to the technical field of medical evaluation and detection. The method comprises an allergen sensitization stage and an allergen excitation stage, in the allergen sensitization stage, an MC903 ethanol solution containing OVA is smeared on the skin of an experimental animal, and in the allergen excitation stage, OVA dissolved in a buffer solution is used for intragastric administration excitation. The method of establishing the skin-sensitized food allergy animal model has the advantages of being easy and convenient to operate, short in modeling period, low in cost, good in reproducibility and the like.

The invention discloses a method for collecting and establishing a mine internal model based on HoloLens glasses. The method comprises the following steps that 1, mine internal structure information is collected by adopting a space mapping function of the HoloLens glasses; 2, the HoloLens glasses transmit a plurality of groups of mine internal structure information grids and photo information stored in the HoloLens glasses to a data processing computer; and 3, the data processing computer analyzes and processes the multiple groups of mine internal structure information grids and the mine innerwall photo information to obtain a mine internal 3D model. The method is simple in step, novel and reasonable in design, easy and clear to operate, good in universality, short in modeling period, good in modeling authenticity, high in practicability, good in using effect and convenient to popularize and use.

The invention discloses a method for establishing a meriones meridianus hepatic alveolar echinococcosis animal model. By adopting a series of technical means, such as screening of experimental animals, dissection and washing of cysts, preparation of protoscoleces suspension, microscopic examination of protoscoleces, anesthesia of meriones meridianus and modeling, the method for establishing the meriones meridianus hepatic alveolar echinococcosis animal model is creatively provided. According to the hepatic alveolar echinococcosis animal model established by means of the method, 60 d cyst tissue almost covers the whole thoracic cavity and completely covers the lung, and 15d, 45d and 60d meriones meridianus cyst coefficient is significantly different from the BALB / c mice cyst coefficient. The provided method for establishing the meriones meridianus hepatic alveolar echinococcosis animal model has the advantages of being high in molding rate, short in molding cycle, low in cost and good in repeatability, and the method in widely used in the medical technical field and also practical for the medical technical field.

The invention provides a method for preparing organoid spheroids. The preparation method comprises: passing cell-containing Matrigel and fluorine oil into a three-way device respectively to obtain cell spheroids, and forming organoid spheroids after culturing; the present invention provides The preparation method of organoid spheroids uses microfluidic method to generate monodisperse biomaterial organoids, which realizes high-throughput production. The size, shape and structure of organoids are controllable and uniform, compared with 2D tumor sensitivity Predictive model, the organoids prepared by the present invention combine tumor microenvironmental factors to predict more accurate results; compared with the PDX mouse tumor sensitivity prediction model, the present invention has a shorter modeling period and lower cost; compared with ordinary Gene sequencing, the clinical prediction rate of the present invention is higher; the preparation method of organoids provided by the present invention is of great significance and value for clinical application, and provides an important basis for the detection of related results.

The invention discloses an application and construction method of a high-selenium induced insulin resistance animal model. The application and construction method comprises the following steps of: feeding 100mug / kg-200ug / kg of selenium to the stomach of an SD (Sprague Dawley) rat every day; and six weeks later, obtaining the insulin resistance animal model. According to the statistic analysis of glucose tolerance and insulin tolerance, insulin signal transduction and gluconeogenesis key enzyme test, the construction of the insulin resistance animal model by utilizing sodium selenite is feasible; and the insulin resistance animal model can be used as a high-selenium induced insulin resistance animal model and a pathogenesis and drug action mechanism research model of diabetes mellitus II. The insulin resistance rat model constructed by the application and the construction method disclosed by the invention has the advantages of simpleness in modeling method, high model establishing success rate, low cost, short period, good reproducibility and the like.

The invention belongs to a power device system in the technical field of aviation, and relates to a method for correcting the installation thrust of an engine model. The method comprises the following steps: (1.1) acquiring an engine bench thrust Fi data table; (1.2) constructing an engine bench thrust model Ft by adopting a three-dimensional interpolation algorithm; (1.3) carrying out an air inlet blowing test; (1.4) modeling by using an interpolation method; (1.5) establishing an air inlet loss correction model K1; (1.6) building a power extraction loss coefficient database; (1.7) establishing a power extraction loss correction model K2; (1.8) building a spray pipe afterbody resistance loss coefficient database; (1.9) establishing a spray pipe afterbody resistance loss correction model K3; (1.10) finishing modelling of an engine installation thrust model. The method has the advantages of high calculation accuracy, simulation instantaneity, easiness in implementation, short modeling period and high engineering applicability.

The invention discloses a method for preparation of a liver fibrosis animal model with juvenile zebrafish. The method adopts juvenile zebrafish as the model animal, and selects diethylnitrosamine andethanol as the modeling drug. The zebrafish genome adopted by the method provided by the invention is similar to the human genome, and presents physiological characteristics very similar to human, andcan be well applied to study of various human diseases. Also the method has the advantages of short modeling cycle, low cost and simple operation. The obtained model can be applied to high throughputscreening of various drugs.

The invention discloses a method for screening genes related to early liver injury of passive smoking rats. By the method, a rat passive smoking model is established. Through a liver gene chip technology, differential genes of early liver injury in passive smoking rats were screened out, and functional annotation and analysis of the differential genes are performed, thus providing gene-level discovery and explanation for liver injury caused by smoking. The established rat passive smoking model has higher scientificity and stronger reliability, is accurate in controlling the concentration of poisoned smoke, has short modeling period, is of great clinical significance, and can realize early detection and early prevention.

The invention provides a construction method of a concanavalin A induced mouse xerophthalmia model and application. The construction method of the mouse xerophthalmia model induced by the concanavalin A comprises the following steps that 10 microgram / milliliter of a PBS solution of the concanavalin A is prepared, the PBS solution is sub-packaged into 20 microliter / drop, one drop is sucked by using an insulin injection needle when the PBS solution is used, and the drop is injected into a lacrimal gland on one side of a mouse, the used mouse strain is a common BALB / c mouse, the price is lower, and the model forming rate is 70% or above. The method is a single injection method, the medicine utilization rate is low, the molding period is short, the xerophthalmia model induced by the method accords with part of clinical characteristics of the xerophthalmia, and the xerophthalmia mouse model can be applied to research and development of medicines for treating xerophthalmia.

The invention provides a method for evaluating the safety of meat products by using zebrafish. Firstly, the meat product is treated with bionic digestion and extraction to obtain the meat sample extract, and then the processing time of the meat sample extract is determined to be 24 hours. The treatment concentration was 1.4-5.6 mg / mL to construct a zebrafish model and evaluate the safety of meat products. The zebrafish model constructed by the present invention and the method for evaluating the safety of meat products have high detection accuracy, the detection method is scientific and reasonable, and the short modeling cycle greatly reduces the detection cost, which provides a basis for popularizing and popularizing the detection method to the general public, enterprises and governments. Realistic feasibility.

The invention relates to the technical field of medical engineering, and particularly relates to a human-derived chordoma bone in-situ PDX model construction method and application thereof. The human-derived chordoma bone in-situ PDX model construction method comprises the steps that (1), chordoma patient tissue is selected; (2), the chordoma tissue is divided into three parts, one part is used for transcriptome sequencing, the other part is used for making a tissue chip, and the last part is implanted into the tibia medullary cavity of an immunodeficiency mouse; (3), the growth condition of the tibia tumor of the mouse is observed; (4), passage is conducted; and (5), the effectiveness and feasibility of the method are judged, and the method is used for detecting curative effect prediction and curative effect monitoring of potential new drugs. The method has the advantages that a model obtained through a chordoma construction method in the prior art cannot well simulate the disease characteristics and cannot well conduct drug screening to obtain effective drugs, the defects are overcome, and the model obtained through the construction method has the advantages of being high in simulation degree and good in feasibility.

The invention relates to a method for inducing a non-alcoholic fatty liver disease model through fluorine intervention. The method comprises the steps that high-fat diet is combined with fluorine intervention to induce the non-alcoholic fatty liver disease of an animal, the fat content in the high-fat feed is 45-60%, 0.01%-0.2% of sodium fluoride is added into the high-fat feed, or 50-1000 mg / L of sodium fluoride is added into drinking water of the animal, and the intervention period is 2-3 months. The non-alcoholic fatty liver disease animal model is high in modeling rate, stable in performance, low in cost and short in period, and the non-alcoholic fatty liver disease can be systematically and scientifically researched by utilizing the method.

The invention discloses a calculation method for obtaining an antenna arm vibration abatement response. According to the antenna arm vibration abatement scheme of a piezoelectric material, the stress of the piezoelectric material is equivalent to an internal moment, and the strain of the piezoelectric material is equivalent to a displacement difference to obtain an analytic coupling calculation model of satellite dynamics, vibration abatement and gesture control. Through the resolving of the model, the antenna vibration frequency response and a time response after vibration abatement can be obtained, the layout analysis of the piezoelectric material on the antenna arm is realized, and the active vibration abatement affect analysis of the antenna arm is realized. An obtained calculation model is a coupling system model which contains the satellite dynamics, the vibration abatement and the gesture control on a top layer, and the cross coupling of each analysis link is fully considered to finish response calculation and also realize the analysis of a vibration transfer mechanism. The stress of the piezoelectric material is equivalent to the internal moment, the strain of the piezoelectric material is equivalent to the displacement difference, and therefore, the method has the advantages of being simple, practical, short in modeling period and convenient in engineering application.

The invention relates to the field of experimental animal models, in particular to a construction method of an ulcerative colitis animal model. The construction method specifically comprises the steps that after a test animal is empty for a period of time, citrobacter and dextran sodium sulfate in a specific proportion are administered every day, and after administration is carried out for 3-5 days, the ulcerative colitis animal model is obtained. According to the novel construction method of the ulcerative colitis animal model, the mouse immune function is reduced through citrobacter, and then dextran sodium sulfate is combined for inflammation, and the problems that an existing ulcerative colitis animal model is prone to self-healing, long in modeling period, high in cost and low in success rate can be solved.

The invention discloses a model for inducing drug resistance in a pseudomonas aeruginosa animal as well as a construction method and an application of the model. With the application of the model constructed by the method, better simulation of a phenomenon of PA infection in respiratory tract of a patient who accepts wide-spectrum antibiotics clinically for a long time can be achieved, and development and change rule of the drug resistance of pseudomonas aeruginosa in a mouse body can be observed, so as to simulate the change of the bacterium (the pseudomonas aeruginosa) in the body and to facilitate study on antibacterial drug-resistance medicine treatment; and meanwhile, the animal model, as a lung index evaluating animal model, is more intuitive, accurate in evaluating result, short in construction cycle and high in efficiency.

The invention relates to a construction method of a mouse talaromyces marneffei latent reactivation model, which comprises the following steps: constructing a TM infected mouse, performing immunosuppression intervention on the TM infected mouse, and finally detecting the TM latent reactivation state of the mouse to realize the construction of the model. The talaromyces marneffei latent reactivation model of the mouse is successfully established for the first time, and the TM latent reactivation model is established in the mouse body, so that the dynamic process of fungi in the human body is simulated, and a model basis is provided for researching the latent infection state of TM in the body. And the TM latent activation model is used as a tool model for researching the TM, so that the molecular mechanism of TM latent infection can be further clarified conveniently, a sufficient basis and an early-stage basis are provided for analyzing the pathogenesis of the TM, a tool basis is also provided for deeply excavating the TM latent infection mechanism, and the research and development of related drugs in the field are promoted.

The invention discloses a method for constructing a urinary calculus animal model by using a locust, and belongs to the field of biomedicine. The method comprises the following steps of (1) injecting4-8 [mu]L of a copper chloride solution with the concentration of 5.0-10.5 mM into the abdominal cavity of the locust for the first time, and feeding the locust with fresh wheat seedlings under the conditions that the ratio of illumination time to darkness time is 13: 11 hours, the illumination temperature is (24-28)+ / -2 DEG C, the darkness temperature is (20-26)+ / -2 DEG C and the humidity is 60-85%; (2) injecting 4-8 [mu]L of the copper chloride solution into the abdominal cavity of the locust for the second time, and feeding the locust with the fresh wheat seedlings under the conditions thatthe ratio of illumination time to darkness time is 14: 10 hours, the illumination temperature is (24-28)+ / -2 DEG C, the darkness temperature is (20-26)+ / -2 DEG C and the humidity is 60-85%; and (3) after the locust injected with the copper chloride solution is subjected to physiological metabolism for 24-48 hours, gradually generating calcium oxalate crystals in different forms in a Markov tube.The method has the advantages of low material cost, good stability, controllable process and short period.

The invention provides a schisanhenol derivative with structures represented by general formulas (1), (2), (3) and (4), a preparation method and application thereof. According to the schisanhenol derivative, an active natural product schisanhenol is used as a substrate, and related groups are introduced to synthesize a series of schisanhenol derivatives; it is disclosed for the first time that theschisanhenol derivative has an obvious protective effect on concanavalin A induced HL-7702 human hepatocyte immune injury, it is prompted that the compound can be used for preparing liver protectiondrugs, and the liver protection effect is further explained through biological tests; schisanhenol in the raw materials mainly comes from dried ripe fruits of schisandra chinensis, and is rich in content, high in quality and low in price; and the preparation process is simple in steps, easy to operate and beneficial to large-scale production.