87results about How to "Induced fast" patented technology

An ablative apparatus and associated methods that can be used to treat atrial fibrillation and other cardiac arryhythmias by ablating cardiac tissue is disclosed. When the distal end of the apparatus reaches the tissue to be ablated, an ablation probe driven by a transducer is vibrated. Scratching the tissue with abrasive members, the vibrating ablation probe is capable of mechanically ablating tissues. This mechanical ablation may be utilized to penetrate epicardial fat, thereby exposing the underlying myocardium. The ablative apparatus may then be used subject the exposed myocardium to mechanical ablation, cryoablation, ultrasonic ablation, and/or any combination thereof.

Based on a CRISPR system, the invention provides sgRNAs specifically targeting to a human LAG-3 gene and a method for specifically knocking out the LAG-3 gene in human cells. The sgRNAs provided by the invention can accurately and effectively target to the human LAG-3 gene and precisely knock out the gene, and has excellent characteristics of high efficiency, short period and low cost. The methodcan be used for preparing human T cells having LAG-3 immune checkpoints knocked out, and then the human T cells are used in for tumor immunotherapy.

The invention provides methods and compositions for the modulation of systemic immune responses by transplantation of hematopoietic stem cells transduced with genes encoding antigens and antigen presenting cell regulatory molecules. The invention includes bi-cistronic lentiviral expression vectors adapted for antigen expression in antigen presenting cells for use in DNA vaccines directed against pathogens and tumor antigens as well as for the treatment of autoimmune disease and for the establishment of antigen tolerance.

A method of evaluating the dynamics of caloric restriction (CR). In one embodiment, a method of evaluating the dynamics of CR comprises obtaining control data from an administration of a long-term control diet program. Each of several mammalian sample groups is subjected to a CR diet program for a different amount of time. The effect of CR between each of the several mammalian sample groups and the control data are compared to each other. Additionally, the effects among members of CR among the several mammalian sample groups are compared to each other.

The invention relates to a method applicable to on-scale production for haematococcus pluvialis green cell preservation and later astaxanthin induction, and belongs to the field of biotechnology. The method provided by the invention comprises the following steps: firstly, performing concentration, mixing and centrifugation to prepare haematococcus pluvialis green cell alga slurry with a freeze-preservation agent and skimmed milk, performing pre-cooling at 4 DEG C, and performing freeze-preservation at -20 DEG C; performing water-bath unfreezing on preserved alga cells, performing freeze-preservation agent elution, performing low-nitrogen weak-light culture to activate alga cells, and performing nutrition deficiency and mixed carbon source addition, thereby achieving rapid induction of green cells and accumulation of astaxanthin. The method is simple to operate and applicable to large-scale production application, the dilemma that production of a production enterprise is halted in winter can be effectively solved, astaxanthin can be induced in spring from green alga cells produced in winter, the production efficiency of equipment and operators can be improved, and the yield of the astaxanthin can be increased.

The invention relates to a method for improving rapid differentiation and bud formation of anthurium andraeanum callus, and relates to a culture method of authurium andraeanum seedling, which comprises the following steps that: A. the anthurium andraeanum variety which is difficult to induce the callus or the callus of which is difficult to be differentiated to adventitious buds is selected as seed; B. watering and fertilizer and medicine application are stopped for one week before the seed production, and the leaf surface is maintained clean; C. the leaves which are just unfolded but is not changed are selected as explant materials; D. the callus is induced; E. adventitious buds are differentiated. Different culture medium formulas and different cutting technologies are used at different key stages during the induction of the anthurium andraeanum callus and the bud differentiation so as to realize the rapid induction and rapid bud differentiation. By adopting the method, the clone of the anthurium andraeanum varieties which are difficult to develop is effectively established, and the development of the factory-oriented production of the test tube plantlet of different anthurium andraeanum varieties can be realized.

The invention relates to a method for rapidly inducing broomrape germination of sunflowers under indoor conditions, in particular to a culture vessel filter paper method and an application thereof to identification of the broomrape resistance level of the sunflowers. Broomrape seeds are induced to germinate on an indoor culture vessel, moisture is preserved by filter paper, the germination condition of the seeds can be directly observed by a microscope, culture is continued for 3 weeks, and broomrape parasitism wart structures of the sunflowers can be observed with naked eyes. Compared with a nutrition bowl method, the steps of sunflower planting and later fine root counting, operation is facilitated, observation is easily realized, and time is greatly saved. A system can be built to rapidly screen and indentify the broomrape resistance level of different sunflower varieties, and serves as a premise and a basis for broomrape resistance breeding of the sunflowers. The identification result of a culture vessel filter paper method is effectively consistent with a field test result, so that the broomrape resistance level of the different sunflower varieties can be rapidly identified.

The invention relates to a hydroxyapatite hollow micro-carrier material and a preparation method thereof. The method comprises the following steps: immerging a cleaned and dried hollow glass microsphere into a NaOH solution and soaking under the continuous agitation; taking out the hollow glass microsphere, drying and immersing the hollow glass microsphere into a container containing 1.5*SBF (Simulated Body Fluid) solution of bioactive glass for soaking and activating at a constant temperature; and immerging the hollow glass microsphere into the 1.5*SBF solution for soaking and depositing at a constant temperature. The hydroxyapatite hollow micro-carrier material and preparation method thereof have the benefit that the obtained material is small in density, HA (Hyaluronic Acid) on the surface is compact and uniform, a coating is weakly-crystallized HA containing carbonate and has good functions of promoting cell adhesion, multiplication and differentiation, and the components of the coating are more close to human bones to facilitate the growth of cells; and meanwhile, the coating is not limited by matrix shape, and the process is simple and easy to operate.

The invention discloses a production method of spherical basic cobalt carbonate with a controllable particle size, and the production method comprises the following general steps of: (1) preparing ofa solution; (2) magnetic inducing nucleation; (3) grain growth; (4) filter pressing and washing; and (5) drying. According to the method, nucleation supersaturation is reduced by applying of an external magnetic field during the synthesis of the basic cobalt carbonate, the induction nucleation period is shortened, and a large amount of basic cobalt carbonate crystal nuclei are instantly formed inthe solution; meanwhile, the quality of the product can be well adjusted by controlling the stirring speed, the reaction temperature and the adding speed of a cobalt liquid, so that the effects of uniform particles and spherical morphology are achieved, and the cobalt content can reach more than 52%. The basic cobalt carbonate product has standard normal distribution of particle size, uniform particle size and good fluidity, and the maximum D50 exceeds 40mu m, the uniformity and stability of the particle size of large-scale continuous produced spherical basic cobalt carbonate can be realized,and the production method simple process, easy operation and low cost, and provides a high-quality raw material for the production of high-end lithium batteries.

The invention discloses a non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs. The non-woven container cutting seedling method includes the following steps: (1) preparing cutting media: the cutting media are formed by preparing the following raw materials according to the volume percent: 15-25% of perlite, 5-15% of vermiculite, 65-75% of turfy soil, 2-7% of bagasse and 3-8% of saw dust, and spraying carbendazim solutions on the cutting media for disinfection; (2) preparing rooting agents: dissolving 100-400mg naphthylacetic acid in 40-50mL 95% ethyl alcohol, filling in 20-30mg 2,4-dichlorophenoxyacetic acid, 5-10mg zinc nitrate, 5-10mg nitric acid molybdenum, 5-10mg lanthanum nitrate and 40-50mg boric acid, filling in water until the solutions are at 1,000mL, and evenly mixing the solution; (3) preparing and treating cutting slips; (4) inserting the cutting slips into the cutting media, wherein the cutting depth is 8-9cm; (5) moving the non-woven fabric seedling raising container into a greenhouse, and erecting a shading screen for shading, wherein the shading percentage is 60-70%, the indoor temperature is kept at 26-29 DEG C, and the humidity is kept at 85-90%. The non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs improves the survival rate and ensures to cultivate robust high-quality seedlings.

The invention relates to a tissue culture and bud induction method for peonies, which is characterized in that the method comprises the following steps: a. peony plants are processed with bactericide, and filled with substrate, kept wet with plastic foil and refrigerated; b. when dormancy of peonies is about to be relieved, outer leaves of bulbils are removed, and the peonies are disinfected and washed by sterile water; c. the bulbils are peeled in an aseptic environment, 2-4 small leaves at basal part of the bulbils in buds and tips of upper leaves in buds are cut off, and the remainder parts are inoculated in solid substrate to be cultured; d. after inoculation the explants are placed in an environment with culture temperature in the range from 23 to 27 DEG C and with certain illumination to be cultured to form aseptic buds. The invention is characterized by easy disinfection, little pollution of mixed bacterium, and a rapid speed to obtain peony aseptic buds.

The invention relates to an induction and detection method for androgenesis dihaploid of Paralichthys olivaceus. The induction and detection method provided by the invention has the beneficial effects that nuclear genetic materials in eggs can be deactivated by only carrying out cold-shock treatment on the fertilized eggs for a certain time, the effect the same as that achieved by ray irradiation deactivation in the traditional androgenesis induction techniques is achieved; and hydrostatic treatment is adopted, the androgenesis dihaploid of the Paralichthys olivaceus can be rapidly prepared.

Disclosed are devices and methods for body temperature management using a heat transfer catheter carrying a heat transfer fluid to heat or cool a desired, focused portion of a patient's body. In accordance with certain aspects of the invention, the heat transfer catheter may include hollow nodes that have greater rigidity than the remainder of the catheter to aid in placement of the heat transfer catheter within and navigation of the desired portion of the patient's body. Methods of using such heat transfer catheter to effect temperature management of the patient's body are also disclosed.

The invention relates to the field of genetic transformation, specifically to a wild tomato Solanum sitiens somatic embryo induction method and a somatic embryo genetic transformation method. The induction method provided by the invention comprises the following steps: (1) culturing Solanum sitiens aseptic seedlings;(2) carrying out dark culture and inducing roots, stems (without axillalry buds) and leaves of Solanum sitiens on a embryogenic callus induction medium ECIM to generate embryogenic callus;(3) transferring the generated embryogenic callus onto a somatic embryogenesis induction medium SEIM in the light to induce somatic embryogenesis; and (4) inducing budding and seedling.

The invention provides a sgRNA specifically targeted to a human TIM-3 gene and a method for specific knock-out of the TIM-3 gene in human cells based on a CRISPR system. In virtue of sgRNA provided bythe invention, the human TIM-3 gene can be accurately and effectively targeted and be precisely knocked out, so the sgRNA has the excellent characteristics of high efficiency, short period and low cost. The method can be used for preparing human T-cells in which TIM-3 immune check points are knocked out, and the prepared human T-cells can be applied to immunotherapy of tumors.

The invention discloses a preparation technology of porous calcium gluconate tantalite/nono-funicular hydroxylapatite bioactive coating. The preparation technology comprises the following step of firstly preparing calcium and phosphorus-containing gluconate tantalite-based complex phase layer on the surface of tantalite by a micro arc oxidation technology, i.e. taking calcium and phosphorus-containing water solution as electrolyte, carrying out the micro arc oxidation treatment on the tantalum under high voltage by using a pulse power source, and carrying out the hydro-thermal treatment on the calcium and phosphorus-containing gluconate tantalite-based complex phase layer under a special hydro-thermal environment condition. The obtained coating with a double-layer structure has the following structure and performance characteristics: an inner layer (which is adjacent to a base body) is a gluconate tantalite-based complex phase layer which consists of gluconate tantalite, and a trace amount of antalum pentoxide and tantalum monoxide and is of a microporous structure in shape; and a surface layer is nono-funicular hydroxylapatite, which is inclined to or in parallel with the gluconate tantalite-based complex phase layer. No incontinuous interface exists between the coating with the double-layer structure and the base body, so that the coating is high in combining strength, and the osteolith can be quickly formed in the pseudo body fluid environment in an inducing way, so that the coating is good in biological activity.

The invention provides an inducer for in-vitro rapidly inducing neutrophil to generate extracellular traps and an induction method thereof. The inducer is prepared from the following components: a pretreatment component A which is selected from IgG2 and IgG4; an induction component B which is selected from APD or a mixture of APD and PMA; and lipopolysaccharide. Meanwhile, the invention also provides an inducation method for in-vitro rapidly inducing the neutrophil to generate extracellular traps (NETs). The induction method comprises the following steps: separating peripheral blood neutrophil; and using the inducer comprising the component A, component B and lipopolysaccharide to induce the neutrophil to form NETs. The inducer and induction method provided by the invention have good application prospect in the aspects for rapidly detecting mammal NETs and screening for promoting or inhibiting the NETs to generate a drug.

The invention provides an inducible promoter capable of synchronously responding to induction of salicylic acid (SA) and jasmonic acid (JA). The inducible promoter is a novel synthetic promoter obtained by inserting a JA responding element in a PR-1a promoter by using a nest type PCR amplification method with a tobacco PR-1a promoter as a basic skeleton, and is named as PR-1a-JA. An inducible activity analysis result shows that the PR-1a-JA can synchronously respond to the induction of SA and JA, has higher SA inducible activity, and can respond to the induction of pathogenic bacteria; responding parts are mainly concentrated in an infection part. The inducible promoter has the advantages of no background expression and no induction from abiotic stress such as high temperature, low temperature, high salt and mechanical injury.

The invention relates to a method for sealing of a circumferentially closed canal. A closure element is secured to one free end of a light guide conducting a laser beam. The light guide with the closure element is introduced into the canal. The closure element is positioned in the region of the canal to be sealed and after positioning of the closure element energy is introduced, so that the closure element melts or softens and remains in this position in the canal and seals it tightly.

The invention relates to a shallow needle oriented to insomnia treatment and a using method thereof. The shallow needle comprises a needle tail, a needle handle, a needle root, a needle body and a needle point from top to bottom. The needle tail and the needle handle are both composed of tightly-spiral stainless steel wires or copper wires, and the needle tail is horizontally arranged at the upper end of the needle handle. The needle root is located at the position where the needle handle is connected with the needle body, and the needle point is located at the lower end of the needle body. According to the using method of the shallow needle, the needle point is ejected on the acupuncture point, the needle tail is slightly pressed with the finger pulp of the thumb of the right hand, and the needle handle is vertically scraped and pushed with the fingernail of the middle finger of the right hand. The vertical scraping and pushing include upward scraping and downward pushing, the upward scraping direction is upwards from the lower end of the needle handle to the top end of the needle handle, and the downward pushing direction is downwards from the top end of the needle handle to the lower end of the needle handle. Vertical and continuous scraping and pushing are performed in the way in a reciprocating mode till the scraping and pushing are completed and stopped. The shallow needle can quickly and accurately induce alpha waves of 3-12 hertz, enables a person to be in a static state, the operating frequency of the shallow needle conforms to the best operating frequency of a doctor, and relief of the operating burden of the doctor is facilitated.

The invention relates to a composite adjuvant for an animal vaccine, a preparation method and the vaccine. In one embodiment, the composite adjuvant is made by emulsifying a composition of squalene, SPAN85, and TWEEN 80. In another embodiment, the composite adjuvant is made by emulsifying a composition of squalene, SPAN85, TWEEN 80, and a saponin. By utilizing the method provided by the invention, the animal vaccine adjuvant can cooperate with the antigen for use at a relatively high ratio and has good biocompatibility and stability.