Tissue culture and bud induction method for peony

A technology of tissue culture and tree peony, applied in the field of plant tissue culture, can solve the problems of death, callus differentiation into buds, long culture period, etc., achieve easy sterilization, promote the rapid propagation process of peony in vitro, and prevent bacterial contamination little effect

Inactive Publication Date: 2011-06-08
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

[0005] The purpose of the present invention is to provide an improved method for inducing bud formation by tissue culture of tree peony, which can overcome the difficulty in explant cont...

Method used

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Embodiment

[0020] Before the peony germinates, dig it out from the field, remove all the soil, cut off the diseased, residual roots, diseased, residual branches and budless branches of the plant, soak the whole plant to be treated with chlorothalonil fungicide solution, and then fish it out. After drying, use moss and sawdust as the cold storage substrate, disinfect by watering, stir while pouring, thoroughly mix the chlorothalonil fungicide solution and the substrate, and keep the substrate humidity at 60%. First put a small amount of substrate at the bottom of the plastic bag, then bundle 2 to 3 peony plants into a group and place them in it to prevent the peony buds from being injured, fill the surroundings of the plants with sterilized substrate to prevent the plants from dehydrating and drying up, and then tie the bag tightly Open the mouth, and evenly tie four 0.5-1.0cm ventilation holes around the plastic bag; finally move the peony plants to the cold storage or refrigerator for re...

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Abstract

The invention relates to a tissue culture and bud induction method for peonies, which is characterized in that the method comprises the following steps: a. peony plants are processed with bactericide, and filled with substrate, kept wet with plastic foil and refrigerated; b. when dormancy of peonies is about to be relieved, outer leaves of bulbils are removed, and the peonies are disinfected and washed by sterile water; c. the bulbils are peeled in an aseptic environment, 2-4 small leaves at basal part of the bulbils in buds and tips of upper leaves in buds are cut off, and the remainder parts are inoculated in solid substrate to be cultured; d. after inoculation the explants are placed in an environment with culture temperature in the range from 23 to 27 DEG C and with certain illumination to be cultured to form aseptic buds. The invention is characterized by easy disinfection, little pollution of mixed bacterium, and a rapid speed to obtain peony aseptic buds.

Description

Technical field: [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing bud formation through tissue culture of peony. Background technique: [0002] Peony (Paeonia Suffruticosa) is a perennial shrub belonging to the Paeoniaceae family and the genus Paeoniae. It is a traditional famous flower in my country. Due to the problems of low seed setting rate, strong seed variability, and low asexual reproduction coefficient in the traditional breeding methods of peony, it is still difficult for my country's peony production to meet the growing social needs, and the lagging of the breeding method also limits the germplasm resources of peony. Progress in preservation and breeding of new varieties. [0003] Using plant tissue culture technology, within a certain period of time, small plants that are hundreds of times or even tens of millions times more similar to the parent's traits can be grown from an explant in vitro. Th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 殷丽青胡永红王新其黄卫昌
Owner SHANGHAI ACAD OF AGRI SCI
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