63 results about "Anthurium andraeanum" patented technology

Two special water-cultured flower 'Hongzhang' nutrient fluid formulas are used in nutrient growth period and generative growth period. It adopts large amount of elements, microelements and hormone, which prevent root bacteria infringing and improve rooting. It utilizes solid package and is simplified and can be used for industrial production.

The invention relates to a method for improving rapid differentiation and bud formation of anthurium andraeanum callus, and relates to a culture method of authurium andraeanum seedling, which comprises the following steps that: A. the anthurium andraeanum variety which is difficult to induce the callus or the callus of which is difficult to be differentiated to adventitious buds is selected as seed; B. watering and fertilizer and medicine application are stopped for one week before the seed production, and the leaf surface is maintained clean; C. the leaves which are just unfolded but is not changed are selected as explant materials; D. the callus is induced; E. adventitious buds are differentiated. Different culture medium formulas and different cutting technologies are used at different key stages during the induction of the anthurium andraeanum callus and the bud differentiation so as to realize the rapid induction and rapid bud differentiation. By adopting the method, the clone of the anthurium andraeanum varieties which are difficult to develop is effectively established, and the development of the factory-oriented production of the test tube plantlet of different anthurium andraeanum varieties can be realized.

The invention discloses an in vitro tissue cultivation method of potted anthurium andraeanum varieties. A leaf blade, a leaf stem, a flower head, a spathe blade or a lateral bud is taken as an explant material; after the explant material is sterilized, the explant material is inoculated to an appropriate culture medium and cultivated for 40-60 days, and then dedifferentiates to form a callus, and the callus rate can reach 87.5%; the material is continuously cultivated for 40-60 days and differentiates again to form an adventive bud, and the differentiation rate can reach 94.12%; and a large quantity of tube seedlings can be rapidly cultivated through multiplication, when the tube seedlings grow to certain heights, the tube seedlings are transferred into the culture medium and transplanted after the tube seedlings take root, and the survival rate can be larger than 95%. With the adoption of an in vitro tissue cultivation technology, a regeneration plant can be stably and efficiently obtained through the way that the callus induces the adventive bud, and rapid and mass propagation of the potted anthurium andraeanum varieties can be achieved; and according to the characteristics of different anthurium andraeanum varieties, the appropriate explant material and a culture medium condition are selected, and the method has a high application value in the production of seedlings of excellent anthurium andraeanum varieties.

The present invention discloses a culture medium for tissue culture of Anzuhua flower and its propagation method by using tissue culture seedlings. Said culture medium is characterized by that it contains macro-element components and abscisic acid, and its propagation method includes the following steps: utilizing the above-mentioned culture medium to induce leaf or petiole to produce callus and adventitious bud; successively transferring the callus whose adventitious bud is differentiated to improved MS culture medium to make culture and propagate adventitious bud; transferring the adventitious bud into rooting culture medium and inducing it to root, then using conventional tissue culture seedling transplantation method to transplant the rooted tissue culture seedling into soil matrix.

The present invention relates to a root system nuturalization method of anthurium andraeanum (a Chinese plant) from matrix culture to water culture. The Chinese plant anthurium andraeanum is a terrestrial plate, if its culture is changed into water culture environment, its root system must be naturalized by means of some measures so as to make it be normally grown for a long period in water environment. The invented kernel is characterized by that in earlier stage of water culture the anthurium andraeanum is placed in a regularly oxygen-charged water-culture environment to make root system naturalization until the naturalized anthurium andraeanum grows the aquatic root adaptable to water-culture environment.

Bacillus methylotrophicus, and preparation and applications of a microbial inoculum thereof are disclosed and belong to the technical field of agricultural microbes. The bacillus methylotrophicus HZ-9 is deposited in the China General Microbiological Culture Collection Center on April 13, 2017, and the accession number of the strain is CGMCC No.14022. The antibacterial spectrum of the strain is wide. The strain has strong inhibiting effects on pathogenic bacteria causing anthurium andraeanum bacterial leaf spot, and other pathogenic bacteria such as colletotrichum gloeosporioides, fusarium oxysporum, poplar rot pathogenic bacteria, rhizoctonia solani and botrytis cinerea. The inhibiting effects on the pathogenic bacteria causing anthurium andraeanum bacterial leaf spot are especially significant. The bacillus methylotrophicus can stably colonize anthurium andraeanum root, blades and petiole surfaces. The microbial inoculum prepared from the strain can be applied for preventing and controlling the anthurium andraeanum bacterial leaf spot and other diseases.

The invention discloses a flower forcing method of potted anthurium andraeanum, comprising: monopotassium phosphate solution or the mixed solution of monopotassium phosphate and 6-benzyl-purine is independently sprayed on the anthurium andraeanum plant leaf surface of which the seeding age is 10-12 months, wherein the concentration of the sprayed monopotassium phosphate is 3mg/L, the concentration of the sprayed 6-benzyl-purine is 10-40 mg/L, the spraying amount is 200 ml/ plant, and the spraying time interval is one time per week. The flower forcing method of the invention can ensure that the tissue culture seedling of the anthurium andraeanum blooms 3-6 months before the blooming period, the flowering rate is improved by 50%, and the flower yield of single plant can reach more than 18. In addition, the flower forcing method of the invention has the advantages of high speed, environment protection, low cost, high benefit and the like and can accelerate anthurium andraeanum tissue culture technology to be widely applied.

The invention belongs to the technical field of plant cross breeding, and discloses an anthurium andraeanum body cell cross breeding method based on a protoplast asymmetric fusion technology. The method comprises the following steps: inoculating a disinfected tender leaf or root tip of an anthurium andraeanum tender during the growth onto a culture medium, so as to culture and form callus tissue, and then transferring the disinfected tender leaf or root tip into a liquid induction medium to perform shaking suspension culture, so as to obtain a suspension cell line; inoculating the suspension cell line into a liquid medium containing enzymatic hydrolysate, so as to perform enzymolysis treatment and obtain protoplast, treating donor protoplast through colchicines, and then performing induction through a PEG high ca<2+>-high pH value method, so as to perform protoplast fusion; and transferring a fusion product into a protoplast liquid medium, and transferring an obtained cell cluster onto an induction medium to culture the cell cluster, so as to obtain a complete body cell hybrid plant. The method is simple and easy and high in fusion frequency, can greatly broaden the genetic diversity of anthurium andraeanum, and develops a novel approach for the cross breeding, genetic improvement and new germplasm development of anthurium andraeanum.

The invention relates to the technical field of plant tissue culture, and provides a method for detoxifying culture of anthurium andraeanum to solve the problems that a detoxified tissue culture seedling is high in operation difficulty, low in detoxification efficiency, long in production cycle and the like in production. The method comprises the following steps: explant preparation, induced culture, enrichment culture, variable-temperature heat treatment, differentiation culture, induced culture of a quasi detoxification stock plant, proliferation and differentiation of the quasi detoxification stock plant, growth of a quasi detoxification strain, virus detection, proliferation and differentiation of detoxification materials, and rooting and transplantation of detoxified seedlings. The method for detoxifying culture of the anthurium andraeanum is simple and convenient to operate, high in detoxification material yield and high in speed.

The invention discloses a method for extracting total ribonucleic acid (RNA) from bracts and inflorescences of Anthurium andraeanum. The method comprises the following steps of: grinding young and tender bracts and inflorescences of the Anthurium andraeanum by using liquid nitrogen, standing an RNA extracting solution in a water bath at the temperature of 63 to 67 DEG C for 5 to 8min, adding chloroform A in an amount which is one fifth of the volume of the RNA extracting solution, violently shaking for 15 to 20s again, standing for 5 to 8min at room temperature, centrifuging to obtain a supernatant, treating the supernatant by using chloroform and a 8M LiCl solution, and centrifuging to obtain the total RNA of the bracts and the inflorescences of the Anthurium andraeanum. By the method, the using amount of toxic and harmful medicines is furthest reduced, and the hazard of RNA extraction on human bodies is reduced; high-quality RNA which is adequate and pure and is not subjected to deoxyribonucleic acid (DNA) contamination can be obtained; and the method is easy to operate, and a high-quality product is obtained in a short time.

The invention designs a tissue culture and rapid propagation inoculation method of anthurium andraeanum. The tissue culture and rapid propagation inoculation method comprises the following steps of (1) stripping adventitious buds obtained through differentiation of callus tissue of the anthurium andraeanum from the callus tissue, and inoculating the stripped adventitious buds to elongation culture mediums or rooting culture mediums or propagation culture mediums for culture, wherein culture conditions include the temperature being 25 DEG C, the illumination intensity being 1500-2500Lux and illumination every day being 15-17h, and performing 40-60 d of culture so as to obtain healthy anthurium andraeanum tissue culture seedlings; and selecting the healthy anthurium andraeanum tissue culture seedlings which are 2cm or above in plant height and have two stalk nodes or above from the obtained healthy anthurium andraeanum tissue culture seedlings, in inoculation disks, performing transection at a position 2mm higher than first nodes of the stalk nodes from bottoms to tops of the seedling stalks to form upper parts and lower parts, enabling the upper parts to be inoculated to the rooting culture mediums, enabling the lower parts to be inoculated to the elongation culture mediums for continuous culture, so as to obtain the anthurium andraeanum tissue culture seedlings which grow orderly, wherein the culture conditions are the same as those in the step 1. According to the tissue culture and rapid propagation inoculation method disclosed by the invention, the problem that plants are uneven to grow in the tissue culture and rapid propagation process of the anthurium andraeanum can be solved.

The invention discloses a cultivation method for acquiring virus-free seedlings of anthurium andraeanum lind by combining thermal treatment and stem-tip stripping. The method comprises the following steps of explant treatment; callus induction and proliferation; adventitious bud induction and proliferation; adventitious bud variable temperature heat treatment; stem-tip stripping; adventitious buddifferentiation and proliferation of stem tip callus; virus detection; acquisition of a virus-free adventitious bud material; and acquisition of virus-free seedlings. The method has the beneficial effects that compared with the treatment method directly performing thermal treatment but avoiding stem-tip stripping, the method can obtain the virus-free seedlings with higher virus elimination rate; and compared with the method of directly acquiring a stem tip explant from a potted plant and cultivating the virus-free seedling, the method has the advantage of providing thousands of explants for stem-tip stripping through one plant, and also has the characteristics of low pollution rate, low cost, no damage to a mother plant offering the explant and the like. Through adoption of the cultivationmethod for acquiring virus-free seedlings of anthurium andraeanum lind by combining thermal treatment and stem-tip stripping, a large quantity of strong virus-free seedlings with high virus elimination efficiency can be acquired within a short time, so that the method provides a technology basis for mass reproduction of virus-free seedlings of anthurium andraeanum lind.

The invention discloses a method of increasing cold resistance of anthurium andraeanum by using exogenous calcium. Exogenous calcium with a concentration of 3.0 mmol*L<-1>-5.0 mmol*L<-1> is added into a cultivation nutrient solution during a seedling stage of the anthurium andraeanum, and the cultivation nutrient solution with Exogenous calcium is applied once a week for 2 months. The method provided by the invention is used in anthurium andraeanum cultivation, and can increase the physiological metabolism level of the anthurium andraeanum in a low temperature, and accordingly after the low temperature is over, leaves do not wither or fall, so that the anthurium andraeanum can grow and develop normally in an environment having a temperature 3 DEG C lower than a conventional cultivation temperature, and the product qualities are ensured. The method provided by the invention can effectively increase the cold resistance of the anthurium andraeanum, so as to decrease the energy consumption of anthurium andraeanum production in winter.

The invention discloses a construction method and application of complementary deoxyribonucleic acid (cDNA) of a somatic embryogenesis receptor-like kinase (SERK) coding gene for regulating somatic embryogenesis of anthurium andraeanum. Initiation codon of a coding gene cDNA sequence is located at 19 base pairs, termination codon of the coding gene cDNA sequence is located at 1888 base pairs, and 622 amino acids are coded in total. The tender leaf of a mature anthurium andraeanum plant is taken as an explant, and through three stages of initiating culture, subculture and development culture, a regeneration plantlet is successfully obtained through an indirect somatic embryogenesis method; the induction callus period of the explant is shorter, and the plant has a high survival rate, quick propagation speed and stable heredity and is not easy to generate cytometaplasia; and the whole SERK gene cDNA sequence of the anthurium andraeanum is separated and cloned, and the SERK gene expression in various periods of different culture stages are analyzed at a transcriptional level to deepen the understanding on the somatic embryogenetic mechanism of the anthurium andraeanum, thereby disclosing the SERK coding gene cDNA for regulating the somatic embryogenesis of the anthurium andraeanum and the construction method of the SERK coding gene cDNA, and providing a high reference value for the function of SERK genes in somatic embryo sex transformation.

The invention discloses a culture medium for tissue culture and rapid propagation of anthurium andraeanum. The culture medium comprises a callus induction culture medium, a callus proliferation culture medium, a callus differentiation culture medium, a subculture medium and a rooting culture medium, the anthurium andraeanum tissue culture and rapid propagation seed production method comprises the following steps: (1) preparing explants; (2) inducing calluses; (3) propagating the calluses; (4) differentiating and culturing the calluses; by utilizing the sterilization method disclosed by the invention, the pollution rate is effectively reduced, the culture medium formula is utilized, the callus induction rate is high, the callus differentiation variation is low, the culture period is greatly shortened, the probability of variation seedlings during subculture is reduced, the generations of subculture are prolonged, and the yield is increased.

The invention discloses a preparation method of traditional Chinese medicinal compound fertilizer used for preventing and treating plant diseases and insect pests of anthurium andraeanum. The traditional Chinese medicinal compound fertilizer is prepared from the following raw materials: 60-70 parts of biogas residue, 13-15 parts of ground phosphate rock, 3-4 parts of boric acid, 12-14 parts of attapulgite powder, 16-19 parts of potassium chloride, 3-4 parts of boric acid, 2-3 parts of polyacrylamide, 1-2 parts of sodium lignin sulfonate, 2-3 parts of itaconic acid, 30-34 parts of plant ash, 8-10 parts of soybean meal, 10-14 parts of mulberry leaves, 15-20 parts of a citric acid aqueous solution, 1-3 parts of hydroxymethyl cellulose, 1-4 parts of amino acid, 10-14 parts of lavender, 4-7 parts of celastrus angulatus, 5-8 parts of xanthium sibiricum, 4-8 parts of matrine, 3-6 parts of table salt and 100-110 parts of water. The traditional Chinese medicinal compound fertilizer can be rapidly absorbed and utilized by plants and does not stimulate or damage the plants, due to diversity of the raw materials, nutrients of the traditional Chinese medicinal compound fertilizer can be more comprehensive, the diversity demand of crops on nutrient elements can be met, and alkaline substances and other active substances in traditional Chinese medicines can damage cell membranes, have good inhibiting effect on pathogenic bacteria and also have the effects of repelling insect pests and inhibiting reproduction of insect pests.

The invention discloses a nutritional substrate with a high survival rate for anthurium andraeanum linden family culture. The substrate is prepared from peat, humus, glass beads, mushroom residue, vermiculite, micro fertilizer, traditional Chinese herbal medicine decoction, biogas slurry by mixing, fermentation, sterilization and the control of water content. The nutritional substrate is reasonable in component composition, suitable for the long term nutritional demands of the growth of the anthurium andraeanum linden, the nutrition is sustainable for long time, the nutrition loss is minimum, at the same time, the water and fertilizer preservation is good, the ventilation is good. When the pot of the anthurium andraeanum linden is changed, the substrate can be used to promote the development of the root system and the fast development of the fibrous roots which can grab the soil. The substrate is beneficial to the pot change of the anthurium andraeanum linden. The anthurium andraeanum linden after the pot change is strong in growth momentum, the root system is strong, the leaves are green, the stems are thick, the quality is good, and the nutritional substrate can achieve the strong anti-disease ability.