18 results about "Anthurium andraeanum" patented technology

The invention provides an anthurium andraeanum virus-free culture method. The anthurium andraeanum virus-free culture method comprises the following steps: step 1, pretreatment of explants; step 2, pretreatment of the composite detoxification induction liquid; step 3, photosensitive detoxification; step 4, starting culture of cluster buds; 5, proliferation and rooting culture; and step 6, seedling hardening and transplanting. According to the method, multiple mild and efficient technologies are organically integrated, so that multi-dimensional accurate removal of viruses is realized, and meanwhile, the life vitality of the explant is protected and excited to the greatest extent, so that the method has remarkable advantages in the aspects of detoxification rate, survival rate, seedling quality, stability and the like.

The invention relates to an anthurium andraeanum root promoting maintenance method before transplanting, which comprises the following steps: S1, pretreatment: selecting anthurium andraeanum seedlings meeting transplanting standards and cutting off bad roots; s2, preparing maintenance liquid, wherein the maintenance liquid is prepared from 0.1-0.3% of sodium hypochlorite, 0.2-0.3% of anthurium flower extract, 5-10% of multicoloured leaf extract, 0.8-1.2% of calcium nitrate, 0.3-0.6% of sodium silicate, 0.05-0.1% of lanthanum nitrate, 1.5-2.5% of chitin and 0.5-1.0% of monopotassium phosphate, wherein the sodium hypochlorite, the anthurium flower extract, the multicoloured leaf extract, the calcium nitrate, the sodium silicate, the lanthanum nitrate, the chitin and the monopotassium phosphate are dissolved The root system is immersed in the maintenance liquid, direct current is applied to the maintenance liquid, the voltage ranges from 1.0 V to 1.5 V, the current ranges from 0.1 mA to 0.2 mA, the direct current is applied for 10 min to 20 min every time, the direct current is applied once every 22 h to 26 h, and the operation is repeated for 4 times to 6 times in total. The method can improve the toughness of adventitious roots of anthurium andraeanum and the self-healing ability after damage, and aims at inhibiting the problem that the roots are easy to rot after the hydroponic anthurium andraeanum seedlings are transplanted into soil.

The invention relates to a method for inducing anthurium callus and synchronously differentiating to generate adventitious buds, which comprises the following steps: 1) selecting explants; 2) induced differentiation culture; and (3) subculture multiplication culture: inoculating adventitious buds generated by induced differentiation into a multiplication culture medium for culture to obtain single seedlings with good growth vigor, and controlling the culture time to be 38-42 days. According to the method, the process from callus induction to differentiation seedling formation of the anthurium explant can be completed in the same culture medium, the stages of callus proliferation culture, differentiation culture and the like are not needed, the adventitious buds can be directly obtained from the explant only by culturing for about 40 days, the culture period is greatly shortened, the factory production cost is effectively reduced, and the method is suitable for industrial production. The economic benefit and the social benefit are improved. According to the method, the key technical bottleneck from explant induced dedifferentiation to redifferentiation in anthurium andraeanum tissue culture is solved, the anthurium andraeanum tissue culture period is greatly shortened, and the anthurium andraeanum tissue culture production cost is greatly reduced.

The application provides a method for promoting the growth of an anthurium andraeanum root system. The method for promoting the growth of the anthurium andraeanum root system is characterized in that the anthurium andraeanum plant is subjected to root irrigation with an anthurium andraeanum root growth-promoting liquid to soak the roots of the anthurium andraeanum plant, and the anthurium andraeanum root growth-promoting liquid is an endophytic fungal culture solution of an orange-yellow calanthe. The method for promoting the growth of the anthurium andraeanum root system can effectively promote the growth of the anthurium andraeanum root system, and further promote the rapid growth of the anthurium andraeanum plant.

The invention discloses a gene AaBRC1 for regulating and controlling plant lateral branch development and application, and the gene AaBRC1 is related to plant lateral branch development and is used for regulating and controlling plant types. The nucleotide sequence of the AaBRC1 gene is as shown in SEQ ID No. 1, and the amino acid sequence of the gene coding protein is as shown in SEQ ID No. 2. According to the plant phenotype research after the AaBRC1 gene is knocked out, it is found that the number of lateral branches of the vaccinium bracteatum after the AaBRC1 gene is knocked out is remarkably increased, the length of the lateral branches of the vaccinium bracteatum is remarkably increased, and in other words, regulation and control of the plant type are achieved by inhibiting expression of the AaBRC1 gene; the research finds that the method plays an important role in research and application of directional improvement of plant lateral branch development, and can be widely used for cultivating new varieties of cruciferous crops with different lateral branch states.

The application discloses an AaHDS gene of ananas comosus and application of the AaHDS gene in regulating aroma, and belongs to the field of genetic engineering of plants. The AaHDS gene of the ananas comosus is obtained by PCR amplification and cloning, the DNA sequence of the gene in the ananas comosus with aroma and the ananas comosus without aroma has obvious difference, and the expression amount of the gene is the highest in the inflorescence. The VIGS technology is used to silence the gene, the expression amount of the gene is reduced by 49.1 %, and the contents of alpha-pinene, beta-pinene and eucalyptol are significantly reduced. The AaHDS gene cloned by the application is beneficial to genetic improvement of plant aroma characteristics, and can be used for cultivating more new varieties of the ananas comosus with pleasant aroma.

The invention discloses an anthurium andraeanum flower color regulation gene AaSC71 and application thereof. Based on an anthurium andraeanum genome, an anthurium andraeanum AaSC71 gene with base difference is cloned from an anthurium andraeanum variety not prone to color fading at high temperature and an anthurium andraeanum variety prone to color fading at high temperature. Researches show that the expression quantity of the AaSC71 gene is continuously increased along with development of Buddha bract and reaches the expression quantity peak value in the S5 period, and the expression quantity difference between varieties not prone to color fading at high temperature and varieties prone to color fading at high temperature is remarkable; under the stress of high temperature, the expression quantity of the AaSC71 gene is firstly increased and then decreased, corresponds to the color change trend of a faded variety, and is an important gene influencing the flower color stability. Meanwhile, the tobacco flower color of the overexpressed AaSC71 gene becomes light, and the anthocyanin content is reduced, so that the overexpressed AaSC71 gene can reduce the anthocyanin content of the plant and regulate the flower color, can be used for cultivating more light-color plants, is beneficial to molecular genetic improvement of plant flower color traits, and can be used for cultivating more new varieties of different flower colors.

The utility model discloses an anthurium soilless culture device, and particularly relates to the technical field of soilless culture devices, the anthurium soilless culture device comprises two groups of symmetrical supporting legs, a water circulation component is fixed at one ends, close to each other, of the two groups of supporting legs, and a mounting component is fixed at the upper ends of the two groups of supporting legs; a sunshade water collecting part is fixed to the upper end of the mounting part, the mounting part comprises a mounting plate fixed to the upper ends of the two sets of supporting legs, two symmetrical vertical plates are fixed to the upper end of the mounting plate, a flow divider and a collector are fixed to the ends, away from each other, of the two vertical plates respectively, and the flow divider is connected with a circulating water pump through a water pipe. According to the soilless culture device for anthurium, through mutual cooperation of the water circulation component, the installation component and the sunshade water collection component, the purpose of automatic irrigation can be achieved, rainwater can be collected to flow into the inner cavity of the water tank, the device can supply water to the water tank in rainy days, and water resources are saved.

The invention relates to the field of application of plant growth regulators, in particular to application of 6-benayl aminopurine in preparation of a medicament for improving the low-temperature tolerance of anthurium andraeanum, a method and a composition. The invention provides an efficient, economic and environment-friendly technical scheme for improving the low-temperature tolerance of anthurium andraeanum by systematically optimizing the use concentration and the application mode of 6-BA and the formula of an auxiliary agent. According to the method, the growth and development conditions of anthurium andraeanum in a low-temperature environment are remarkably improved, the cold resistance of plants is improved, energy consumption in the production process is effectively reduced, remarkable economic and social benefits are achieved, related gaps in the prior art are filled, and high innovativeness and application value are achieved.

The application provides a propagation method for tissue culture of anthurium andraeanum, which comprises the following steps: external explant selection and disinfection, external explant cutting, callus induction, proliferation body cultivation, differentiation of adventitious buds, rooting and strong root training, seedling training and transplantation. The composite disinfectant is composed of chlorobromoisocyanuric acid, everlasting flower oil, Tween and distilled water in a specific ratio. Chlorobromoisocyanuric acid and everlasting flower oil produce complementary effects through different antibacterial mechanisms, thereby improving the broad-spectrum and bactericidal effect of the composite disinfectant. Sambucus nigra extract is added to the rooting culture medium, and the coumarin and ferulic acid contained in the sambucus nigra extract can be converted into lignin in the anthurium andraeanum tissue culture seedlings, thereby enhancing the mechanical connection between cells and making the connection between the adventitious roots and the callus more compact. In addition, the polysaccharides and their derivatives contained in the sambucus nigra extract can promote the synthesis and deposition of pectin in the tissue culture seedlings, thereby increasing the adhesion between cells and making the connection between the adventitious roots and the callus more compact.

The application provides a tissue culture and rapid propagation culture medium for an anthurium and a preparation method thereof, the culture medium contains a large amount of elements, trace elements, iron salts, organic components and plant growth regulators in specific weights per liter, and the culture medium is additionally added with an osmotic regulator and an anti-reverse enhancer and the like to improve the effect of tissue culture and rapid propagation of the anthurium. The elderberry extract is obtained by a supercritical extraction method, and the purity and quality of the elderberry extract are ensured, the preparation method covers multiple steps, and includes operations such as sequential dissolution of components, stirring, pH value adjustment, sub-packaging and sterilization and the like, and the key parameters of each step are clear, such as sterilization conditions, a mass-volume ratio of a plant growth regulator to anhydrous ethanol and the like. The scheme provides an optimized culture medium and a matching preparation method for tissue culture and rapid propagation of the anthurium, and is helpful to improve the culture effect of the anthurium.

The application discloses a SNP molecular marker primer for assisting offspring selection in a short vegetative growth period of an anthurium andraeanum and application thereof. The application takes a CDC73 gene differentially expressed in different vegetative growth periods of anthurium andraeanum varieties as a target of a SNP site, and develops a SNP marker primer which is a molecular marker closely linked to the short vegetative growth period, can quickly and accurately select offspring in the short vegetative growth period, and has an accuracy of 70.96% in the assisted selection of offspring in the short vegetative growth period through the SNP marker primer. Meanwhile, a method for assisting selection of offspring in the short vegetative growth period of anthurium andraeanum by using the SNP molecular marker is provided, the target traits of the short vegetative growth period can be quickly and accurately selected in a seedling period, the breeding cycle is shortened, the plants in the non-short vegetative growth period can be directly eliminated, the planting scale is reduced, the breeding efficiency and benefit of the anthurium andraeanum are improved, and a foundation is laid for carrying out molecular breeding of the vegetative growth period of the anthurium andraeanum.

A method for rapid verification of an anthocyanin biosynthesis gene function using Anthurium andraeanum ‘Xueyu’ is provided. An engineered bacterium carrying an anthocyanin biosynthesis-related functional gene AnUFGT1 from Anthurium andraeanum is injected into a young pedicel of the Anthurium andraeanum ‘Xueyu’ once a day for 3 consecutive times using a syringe. The anthocyanin biosynthesis on a white spathe is observed to verify a function of the AnUFGT1 gene in regulating the anthocyanin biosynthesis. The method has a simple process flow, including using a non-blossomed inflorescence of the Anthurium andraeanum ‘Xueyu’ as a material, injecting a bacterial suspension into the material, conducting routine management, and then observing a phenotype after the spathe is unfolded. The method also shows a short cycle taking 15 d to 20 d during the entire process. The method provides an efficient means for rapid verification of partial functional genes.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Streptomyces gracilis* AH001 isolated from *Anthurium stenoptera* and its applications. *Streptomyces gracilis* (… Streptomyces pratensis Streptomyces AH001 was deposited at the Guangdong Provincial Microbial Culture Collection Center on January 14, 2026, with accession number GDMCC No. 67655. Indoor experiments have shown that Streptomyces AH001 has a significant inhibitory effect on various pathogens originating from Anthurium andraeanum, and can be used to prepare highly effective microbial biocontrol agents to ensure biological and environmental safety while controlling Anthurium andraeanum diseases.

The invention discloses an anthurium andraeanum tissue culture seedling propagation and inoculation device which comprises a layered goods shelf, and light sources are arranged at the tops of compartments of the goods shelf. The core of the unit is an inoculation box, and the bottom of the front end of the inoculation box is provided with a drainage pipe and a drainage valve. A partition frame is arranged in the inoculation box, a plurality of slots are formed in the partition frame, plug bushes are detachably arranged in the slots, upper and lower openings are formed in the plug bushes, connecting rods are arranged in the plug bushes to fix a plug box, a first through hole is formed in the bottom of the plug box, and limiting foam is placed in the first through hole. The top of the vertical rod is provided with an adjusting box, the bottom of the box is provided with a second through hole for light transmission, and a shading part can be placed in the box to adjust the light transmission intensity. An illumination intensity sensor is arranged between the plug bush and the second through hole, and an illumination intensity display assembly is arranged at the front end of the compartment and electrically connected with the sensor. In addition, a stirring assembly is further arranged in the inoculation box and used for stirring liquid in the box. According to the device, the inoculation posture of the tender tissue can be ensured, the pH value of a culture solution can be easily maintained, and proper illumination can be easily provided for each tender tissue, so that the survival rate and the superior product rate of propagation inoculation are improved.

The invention discloses a genetic transformation method of anthurium andraeanum, and belongs to the field of biological breeding, the method comprises the steps of callus induction, callus propagation, callus infection and the like, in the method, through treatment of a specific resting culture medium, growth of positive cells is recovered; and screening the calluses through two rounds of propagation, so that the positive calluses can be propagated. The efficiency of anthurium callus infection is improved by improving an infection solution, a co-culture medium, a resting culture medium, a screening culture medium and the like. Besides, in the method, specific pre-culture is carried out on the agrobacterium dip dyeing liquid, expression of related protein infected by agrobacterium is induced in advance, and the infection efficiency of the agrobacterium on the callus can be improved.

The invention discloses a method for improving the vitrification ultralow-temperature preservation effect of anthurium andraeanum calluses. The method comprises the following steps: S1, pre-culturing the anthurium andraeanum calluses; s2, carrying out permeation protection on the pre-cultured anthurium andraeanum calluses by using loading liquid; s3, sucking out the loading liquid, and carrying out dehydration treatment on the percolated and protected anthurium andraeanum callus by using vitrification liquid containing plum blossom dehydrin protein; and S4, carrying out cryopreservation on the dehydrated anthurium andraeanum calluses by using liquid nitrogen. According to the method, 10 [mu] mol / L plum blossom dehydrin protein is added into the vitrification solution to serve as an exogenous additive substance, so that the cell activity after ultralow-temperature cryopreservation of the vaccinium bracteatum calluses is effectively improved, and the ultralow-temperature preservation effect of multiple vaccinium bracteatum varieties can be obviously improved, including Guanghua Ziyun, Xiaolinglin, Xianwang, Jun Zhongjiang and the like.