Root-promoting maintenance method before anthurium andraeanum transplantation

Abstract

The invention relates to an anthurium andraeanum root promoting maintenance method before transplanting, which comprises the following steps: S1, pretreatment: selecting anthurium andraeanum seedlings meeting transplanting standards and cutting off bad roots; s2, preparing maintenance liquid, wherein the maintenance liquid is prepared from 0.1-0.3% of sodium hypochlorite, 0.2-0.3% of anthurium flower extract, 5-10% of multicoloured leaf extract, 0.8-1.2% of calcium nitrate, 0.3-0.6% of sodium silicate, 0.05-0.1% of lanthanum nitrate, 1.5-2.5% of chitin and 0.5-1.0% of monopotassium phosphate, wherein the sodium hypochlorite, the anthurium flower extract, the multicoloured leaf extract, the calcium nitrate, the sodium silicate, the lanthanum nitrate, the chitin and the monopotassium phosphate are dissolved The root system is immersed in the maintenance liquid, direct current is applied to the maintenance liquid, the voltage ranges from 1.0 V to 1.5 V, the current ranges from 0.1 mA to 0.2 mA, the direct current is applied for 10 min to 20 min every time, the direct current is applied once every 22 h to 26 h, and the operation is repeated for 4 times to 6 times in total. The method can improve the toughness of adventitious roots of anthurium andraeanum and the self-healing ability after damage, and aims at inhibiting the problem that the roots are easy to rot after the hydroponic anthurium andraeanum seedlings are transplanted into soil.

Description

Technical Field

[0001] This invention belongs to the field of Anthurium transplantation and maintenance technology, and specifically relates to a method for promoting root growth and maintenance of Anthurium before transplantation. Background Technology

[0002] Anthuriums, belonging to the genus Anthurium in the Araceae family, are commonly propagated through cuttings. The specific procedure involves selecting leaves from healthy mother plants with intact, robust petioles. After surface sterilization, the base of the petiole is inserted into a transparent container filled with nutrient solution for hydroponic rooting. Environmental parameters must be controlled during the cultivation process, primarily maintaining a suitable temperature range of 18-25℃, a light intensity of 1500-2000 lux, and a fixed photoperiod of 12 hours / day. Plants are considered ready for transplanting when they reach a height of ≥8 cm, have ≥5 adventitious roots, and the shortest adventitious root is ≥4 cm. Once transplanted, the plants are transferred to a soil-based substrate consisting of peat moss, perlite, and coconut coir.

[0003] However, because the adventitious roots of Anthurium are fleshy and brittle, transplanting hydroponically propagated Anthurium seedlings directly into soil can easily cause them to bend or break due to pressure from hard particles such as perlite, leading to root infection and rot. Furthermore, the significant differences in physicochemical parameters (such as moisture, nutrients, oxygen content, and microbial community) between hydroponics and soil cultivation environments can easily trigger root stress, further increasing the risk of root rot. Summary of the Invention

[0004] This invention provides a method for promoting root growth and maintenance before transplanting Anthurium, which can improve the toughness of roots in hydroponic Anthurium seedlings and their self-healing ability after root damage.

[0005] The technical solution adopted in this invention:

[0006] A method for promoting root growth and maintaining Anthurium roots before transplanting, comprising the following steps:

[0007] S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove any unhealthy roots;

[0008] S2. Preparation of the maintenance solution: The maintenance solution comprises, by mass-volume ratio g / mL, 0.1-0.3g sodium hypochlorite, 0.2-0.3g anthurium flower extract, 5-10g pandanus leaf extract, 0.8-1.2g calcium nitrate, 0.3-0.6g sodium silicate, 0.05-0.1g lanthanum nitrate, 1.5-2.5g chitosan, and 0.5-1.0g potassium dihydrogen phosphate dissolved in deionized water;

[0009] S3. Immersion maintenance: Immerse the roots in the maintenance solution and apply direct current to the maintenance solution. The voltage is 1.0-1.5V and the current is 0.1-0.2mA. Each time, it is 10-20 minutes. Repeat this every 22-26 hours for a total of 4-6 times.

[0010] Furthermore, during the soaking process in step S3, air is used as an air source to aerate the curing solution for 5 minutes every 15-20 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc is 0.1-0.15 mm, the distance between adjacent aeration holes is 2-3 mm, and the ratio of the volume of aeration per cycle to the volume of curing solution is m. 3 / L is 0.05-0.1.

[0011] Furthermore, the extraction steps for the anthurium flower extract are as follows:

[0012] S211. Collect mature Anthurium flowers, remove damaged parts, wash with deionized water and drain. Crush the flowers to a particle size ≤2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities.

[0013] S212. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity ≥70 μW / cm². 2 Under these conditions, the sample is treated for 10-15 minutes, supplemented with 35KHz ultrasonic treatment, and the power density is controlled at 50-100W / L.

[0014] Furthermore, the extraction steps for the pandan leaf extract are as follows:

[0015] S221. Wash the pandan leaves with deionized water and drain them. Crush the pandan leaves to a particle size ≤2mm and then extract the juice. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities.

[0016] S222. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity ≥70 μW / cm². 2 Under these conditions, the sample is treated for 10-15 minutes, supplemented with 35KHz ultrasonic treatment, and the power density is controlled at 50-100W / L.

[0017] Furthermore, the preparation of the maintenance solution in step S2 includes the following steps:

[0018] S21. Heat 80% deionized water to 40-45℃ and stir continuously at a speed of 100-120 rpm.

[0019] S22. Add sodium hypochlorite, anthurium flower extract, pandan leaf extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin, and potassium dihydrogen phosphate in sequence, with an interval of 5-10 minutes between each component. After adding the last component, add the remaining deionized water and continue stirring for more than 20 minutes.

[0020] Furthermore, in step S3, two electrodes are inserted into the nutrient solution and positioned on either side of the roots of the Anthurium seedling. The closest distance between either electrode and the roots of the Anthurium is at least 5 cm. The power is turned on and the voltage is increased to the target value at a rate of 0.2 V / s. When the preset value is reached, the voltage is decreased at a rate of 0.2 V / s and the power is turned off.

[0021] Furthermore, in step S3, the temperature of the curing solution is controlled at 20-30℃, and the light intensity is maintained at 1500-2000 lux.

[0022] Furthermore, after completing step S3, proceed to step S4: evenly spray castor oil onto the roots of the anthurium, with a spraying amount of 1-2 mL per plant.

[0023] Furthermore, after completing step S4, proceed to step S5: place the Anthurium seedlings in a dark environment for 12-18 hours at a temperature of 22-25℃ and a relative humidity of 70%-80%.

[0024] Furthermore, the term "unhealthy roots" includes rotten roots and dead roots.

[0025] Compared with the prior art, the beneficial effects of the present invention are:

[0026] 1. During the soaking and maintenance process of the anthurium's roots, applying appropriate voltage and current to the maintenance solution can promote the absorption of calcium ions (Ca) while avoiding electrical damage. 2+ Sodium ions (Na) + ) and lanthanum ion (La 3+ The dissociation and migration of ions enhance the absorption efficiency of these ions by the roots by activating the opening of ion channels in root cell membranes and increasing the activity of ATP synthase. This process, through physical stimulation and chemical synthesis, thickens the cell walls of Anthurium roots, increases lignin deposition, and strengthens cell wall cross-linking. Furthermore, under appropriate nutrient composition and electrical stimulation, it also improves the roots' ability to absorb and store nutrients. Therefore, through the synergistic effect of reasonable electrical stimulation parameters and nutrient solution composition, the compressive strength, bending resistance, wound healing ability, and new root germination ability of the root system can be significantly improved.

[0027] 2. The polysaccharides and amino acids in the extract of Anthurium flowers can chelate calcium. 2+ and La 3+ This forms a complex that is easily absorbed by root cells, while the linoleic acid in the pandan leaf extract can activate Na+. + / K + -ATPase, synergistic electrical stimulation is beneficial for increasing the rate of ion transmembrane transport.

[0028] 3. The polyphenols in Anthurium flower extract can cross-link cell wall cellulose, while the squalene in Pandanus leaf extract can penetrate into the interfibrillary spaces of the cell wall. Together, they increase the mechanical strength of the cell wall. Furthermore, the lignin deposition process is regulated by the alkaloids in Anthurium flower extract, which, combined with the antioxidant protection of Pandanus leaf extract, promotes the enhancement of lignin synthase activity and facilitates the formation of a denser protective layer.

[0029] 4. The glucose derivatives in the Anthurium flower extract can be used directly as a carbon source, and the vitamin K3 in the Pandanus leaf extract can activate the glycolysis pathway. The two work together to enhance the energy reserves of root cells. Furthermore, the polyphenols in the Anthurium flower extract and the 2-acetyl-1-pyrrolline in the Pandanus leaf extract can form an antioxidant network to scavenge reactive oxygen species (ROS) generated during electrical stimulation.

[0030] 5. The 2-acetyl-1-pyrrolidone in the variegated leaf extract can stabilize the cell membrane potential and, in synergy with the potential difference generated by electrical stimulation, facilitate the further opening of ion channels; the squalene in the variegated leaves can enhance cell membrane fluidity, promote the transport of the active ingredients in the anthurium flower extract to the root tip meristem, and accelerate the germination of new roots; the linoleic acid in the variegated leaf extract can activate the stress response genes in the roots of anthurium and, in synergy with the physical resistance induction by electrical stimulation, help improve the root system's tolerance to drought and salinity. Detailed Implementation

[0031] To better understand the technical content of this invention, specific embodiments are provided below and the invention will be further explained.

[0032] Example 1

[0033] This invention provides a method for promoting root growth and maintaining Anthurium roots before transplanting, comprising the following steps:

[0034] S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove rotten roots, dead roots and other undesirable roots for later use;

[0035] S2. Preparation of maintenance solution: The maintenance solution includes sodium hypochlorite 0.1 g / mL, anthurium flower extract 0.2 g / mL, pandan leaf extract 5 g / mL, calcium nitrate 0.8 g / mL, sodium silicate 0.3 g / mL, lanthanum nitrate 0.05 g / mL, chitin 1.5 g / mL, and potassium dihydrogen phosphate 0.5 g / mL.

[0036] The extraction steps for Anthurium flower extract are as follows:

[0037] S211. Collect mature Anthurium flowers, remove damaged parts, wash with deionized water and drain, crush the flowers to a particle size of 2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities.

[0038] S212. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 10 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 50W / L.

[0039] The extraction steps for pandan leaf extract are as follows:

[0040] S221. Wash the pandan leaves with deionized water and drain them. Crush the pandan leaves to a particle size of 2 mm and then extract the juice. Collect the juice and filter it through a 0.8 μm microporous membrane to remove impurities.

[0041] S222. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 10 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 50W / L.

[0042] The preparation steps for the maintenance solution include:

[0043] S21. Heat 80% deionized water to 40°C and stir continuously at a stirring speed of 100 rpm.

[0044] S22. Add sodium hypochlorite, anthurium flower extract, pandan leaf extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin and potassium dihydrogen phosphate in sequence, with an interval of 5 minutes between each component. When adding the last component, add the remaining deionized water and continue stirring for 20 minutes.

[0045] S3. Immersion Maintenance: Immerse the roots in the maintenance solution. Specifically, insert two electrodes into the maintenance solution on both sides of the roots of the Anthurium seedling, with the closest distance between any electrode and the root being 5cm. Repeat this process every 22 hours for a total of 4 times. Each time, start the power supply and increase the voltage at a rate of 0.2V / s to the target value of 1.0V, then maintain the output current at 0.1mA for 10 minutes. After that, decrease the voltage at a rate of 0.2V / s and turn off the power supply. The electrodes do not directly contact the roots but rather the maintenance solution. This avoids damage to the roots from the current while providing a uniform current stimulation to the roots through the maintenance solution. Simultaneously, the gradual increase and decrease of voltage avoids damage to the roots from sudden voltage changes.

[0046] During the soaking process, air was used as an air source to aerate the curing solution for 5 minutes every 15 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc was 0.1 mm, and the distance between adjacent aeration holes was 2 mm. The ratio of the volume of aeration per cycle to the volume of curing solution was m. 3The concentration of oxygen and carbon dioxide in the solution is 0.05 L / L. The temperature of the solution during treatment is 20°C, and the light intensity is 1500 lux. Proper aeration settings not only maintain suitable oxygen and carbon dioxide levels in the solution but also prevent sedimentation that could affect root absorption. Furthermore, the application of electricity to the solution and aeration should not overlap. Specifically, electricity and aeration should not be applied at the same time; they should be spaced apart. Specifically, electricity should be applied to the solution at least 2 minutes after aeration ends, or aeration should begin only after electricity has finished. This is to prevent bubbles from bursting near the roots under the influence of current and to avoid voltage spikes near the roots that could damage them.

[0047] S4. Spray castor oil evenly onto the roots of the anthurium, at a rate of 1 mL per plant. The castor oil forms a hydrophobic oil film on the root surface, reducing frictional damage to the roots from perlite particles during transplanting. It also provides short-term antibacterial effects and good air permeability.

[0048] S5. Place the Anthurium seedlings in a dark environment for 12 hours at a temperature of 22℃ and a relative humidity of 70%. The dark environment enhances the accumulation of endogenous hormones, promotes corking of the cut surfaces, and also promotes the transfer of nutrients to the roots.

[0049] Example 2

[0050] This invention provides a method for promoting root growth and maintaining Anthurium roots before transplanting, comprising the following steps:

[0051] S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove rotten roots, dead roots and other undesirable roots;

[0052] S2. Preparation of maintenance solution: The maintenance solution comprises sodium hypochlorite 0.3 g / mL, anthurium flower extract 0.3 g / mL, pandan leaf extract 10 g / mL, calcium nitrate 1.2 g / mL, sodium silicate 0.6 g / mL, lanthanum nitrate 0.1 g / mL, chitin 2.5 g / mL, and potassium dihydrogen phosphate 1.0 g / mL, dissolved in deionized water.

[0053] The extraction steps for the anthurium flower extract are as follows:

[0054] S211. Collect mature Anthurium flowers, remove damaged parts, wash with deionized water and drain, crush the flowers to a particle size of 2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities.

[0055] S212. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 100W / L.

[0056] The extraction steps for pandan leaf extract are as follows:

[0057] S221. Wash the pandan leaves with deionized water and drain them. Crush the pandan leaves to a particle size of 2 mm and then extract the juice. Collect the juice and filter it through a 0.8 μm microporous membrane to remove impurities.

[0058] S222. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 100W / L.

[0059] The preparation steps for the maintenance solution include:

[0060] S21. Heat 80% deionized water to 45°C and stir continuously at a stirring speed of 120 rpm.

[0061] S22. Add sodium hypochlorite, anthurium flower extract, pandan leaf extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin and potassium dihydrogen phosphate in sequence, with each component added 10 minutes apart. After adding the last component, add the remaining deionized water and continue stirring for 20 minutes.

[0062] S3. Immersion Maintenance: Immerse the roots in the maintenance solution. Specifically, insert two electrodes into the maintenance solution and position them on both sides of the roots of the Anthurium seedling. The closest distance between any electrode and the Anthurium root should be 5cm. Repeat this process every 26 hours for a total of 6 times. Each time, start the power supply and increase the voltage to the target value of 1.5V at a rate of 0.2V / s, then maintain the output current at 0.2mA for 20 minutes. After that, decrease the voltage at a rate of 0.2V / s and turn off the power supply.

[0063] During the soaking process, air was used as an air source to aerate the curing solution for 5 minutes every 20 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc was 0.15 mm, and the distance between adjacent aeration holes was 3 mm. The ratio of the volume of aeration per cycle to the volume of curing solution was m. 3 The concentration is 0.1 L / L, the temperature of the curing solution during the treatment process is 30℃, and the light intensity is 2000 lux. The energizing and aeration of the curing solution must not overlap in time. Specifically, the energizing and aeration of the curing solution should not be performed at the same time; they should be spaced apart. Specifically, at least 2 minutes should pass after aeration ends before energizing the curing solution can begin, or aeration can begin only after energizing has ended.

[0064] S4. Spray castor oil evenly at the base of the anthurium, with a spraying amount of 2 mL per plant;

[0065] S5. Place the Anthurium seedlings in a dark environment for 18 hours at a temperature of 25°C and a relative humidity of 80%.

[0066] Example 3

[0067] This invention provides a method for promoting root growth and maintaining Anthurium roots before transplanting, comprising the following steps:

[0068] S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove rotten roots, dead roots and other undesirable roots for later use;

[0069] S2. Preparation of maintenance solution: The maintenance solution includes sodium hypochlorite 0.2 g / mL, anthurium flower extract 0.25 g / mL, pandan leaf extract 7 g / mL, calcium nitrate 1.0 g / mL, sodium silicate 0.5 g / mL, lanthanum nitrate 0.08 g / mL, chitin 2.0 g / mL, and potassium dihydrogen phosphate 0.7 g / mL.

[0070] The extraction steps for Anthurium flower extract are as follows:

[0071] S211. Collect mature Anthurium flowers, remove damaged parts, wash with deionized water and drain, crush the flowers to a particle size of 2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities.

[0072] S212. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 700W / L.

[0073] The extraction steps for pandan leaf extract are as follows:

[0074] S221. Wash the pandan leaves with deionized water and drain them. Crush the pandan leaves to a particle size of 2 mm and then extract the juice. Collect the juice and filter it through a 0.8 μm microporous membrane to remove impurities.

[0075] S222. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 700W / L.

[0076] The preparation steps for the maintenance solution include:

[0077] S21. Heat 80% deionized water to 43°C and stir continuously at a stirring speed of 110 rpm.

[0078] S22. Add sodium hypochlorite, anthurium flower extract, pandan leaf extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin and potassium dihydrogen phosphate in sequence, with an interval of 7 minutes between each component. When adding the last component, add the remaining deionized water and continue stirring for 20 minutes.

[0079] S3. Immersion Maintenance: Immerse the roots in the maintenance solution. Specifically, insert two electrodes into the maintenance solution and position them on both sides of the roots of the Anthurium seedling. The closest distance between any electrode and the Anthurium root should be 5cm. Repeat this every 24 hours for a total of 5 times. Each time, start the power supply and increase the voltage to the target value of 1.2V at a rate of 0.2V / s, then maintain the output current at 0.15mA for 15 minutes. After that, decrease the voltage at a rate of 0.2V / s and turn off the power supply.

[0080] During the soaking process, air was used as an air source to aerate the curing solution for 5 minutes every 17 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc was 0.12 mm, and the distance between adjacent aeration holes was 2.5 mm. The ratio of the volume of aeration per cycle to the volume of curing solution was m. 3 The concentration of the curing solution is 0.07, the temperature of the curing solution during the treatment process is 25℃, and the light intensity is 1700 lux. The energizing and aeration of the curing solution should not overlap in time. Specifically, the energizing and aeration of the curing solution should not be performed at the same time. The two should be separated. Specifically, the energizing of the curing solution should be started at least 2 minutes after the aeration ends, or the aeration should be started after the energizing ends.

[0081] S4. Spray castor oil evenly onto the base of the anthurium, at a rate of 1.5 mL per plant.

[0082] S5. Place the Anthurium seedlings in a dark environment for 16 hours at a temperature of 25°C and a relative humidity of 75%.

[0083] Comparative Example 1

[0084] The difference from Example 3 is the removal of the operation in step S3, which involves "inserting two electrodes into the nutrient solution and positioning them on either side of the roots of the Anthurium seedling, with the closest distance between any electrode and the root of the Anthurium 'Atropurpurea' being 5cm. This is repeated 3 times every 20 hours. Each time, the power is turned on and the voltage is increased to the target value of 1.2V at a rate of 0.2V / s, then the output current is maintained at 0.15mA for 15 minutes. After that, the voltage is decreased at a rate of 0.2V / s and the power is turned off."

[0085] This invention provides a method for promoting root growth and maintaining Anthurium roots before transplanting, comprising the following steps:

[0086] S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove rotten roots, dead roots and other undesirable roots for later use;

[0087] S2. Preparation of maintenance solution: The maintenance solution includes sodium hypochlorite 0.2 g / mL, anthurium flower extract 0.25 g / mL, pandan leaf extract 7 g / mL, calcium nitrate 1.0 g / mL, sodium silicate 0.5 g / mL, lanthanum nitrate 0.08 g / mL, chitin 2.0 g / mL, and potassium dihydrogen phosphate 0.7 g / mL.

[0088] The extraction steps for the anthurium flower extract are as follows:

[0089] S211. Collect mature Anthurium flowers, remove damaged parts, wash with deionized water and drain, crush the flowers to a particle size of 2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities.

[0090] S212. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 700W / L.

[0091] The extraction steps for the pandan leaf extract are as follows:

[0092] S221. Wash the pandan leaves with deionized water and drain them. Crush the pandan leaves to a particle size of 2 mm and then extract the juice. Collect the juice and filter it through a 0.8 μm microporous membrane to remove impurities.

[0093] S222. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 700W / L.

[0094] The preparation steps for the maintenance solution include:

[0095] S21. Heat 80% deionized water to 43°C and stir continuously at a stirring speed of 110 rpm.

[0096] S22. Add sodium hypochlorite, anthurium flower extract, pandan leaf extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin and potassium dihydrogen phosphate in sequence, with an interval of 7 minutes between each component. When adding the last component, add the remaining deionized water and continue stirring for 20 minutes.

[0097] S3. During the soaking process, the curing solution is aerated with air for 5 minutes every 17 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc is 0.12 mm, the distance between adjacent aeration holes is 2.5 mm, and the ratio of single aeration volume to curing solution volume is m. 3 The concentration of the curing solution is 0.07, the temperature of the curing solution during the treatment process is 25℃, and the light intensity is 1700 lux. The energizing and aeration of the curing solution should not overlap in time. Specifically, the energizing and aeration of the curing solution should not be performed at the same time. The two should be separated. Specifically, the energizing of the curing solution should be started at least 2 minutes after the aeration ends, or the aeration should be started after the energizing ends.

[0098] S4. Spray castor oil evenly onto the base of the anthurium, at a rate of 1.5 mL per plant.

[0099] S5. Place the Anthurium seedlings in a dark environment for 16 hours at a temperature of 25°C and a relative humidity of 75%.

[0100] Comparative Example 2

[0101] The difference from Example 3 lies in the removal of the anthurium flower extract from the conditioning solution and its preparation process:

[0102] This invention provides a method for promoting root growth and maintaining Anthurium roots before transplanting, comprising the following steps:

[0103] S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove rotten roots, dead roots and other undesirable roots for later use;

[0104] S2. Preparation of maintenance solution: The maintenance solution includes sodium hypochlorite 0.2 g / mL, pandan leaf extract 7 g / mL, calcium nitrate 1.0 g / mL, sodium silicate 0.5 g / mL, lanthanum nitrate 0.08 g / mL, chitin 2.0 g / mL, and potassium dihydrogen phosphate 0.7 g / mL, dissolved in deionized water.

[0105] The extraction steps for pandan leaf extract are as follows:

[0106] S221. Wash the pandan leaves with deionized water and drain them. Crush the pandan leaves to a particle size of 2 mm and then extract the juice. Collect the juice and filter it through a 0.8 μm microporous membrane to remove impurities.

[0107] S222. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 700W / L.

[0108] The preparation steps for the maintenance solution include:

[0109] S21. Heat 80% deionized water to 43°C and stir continuously at a stirring speed of 110 rpm.

[0110] S22. Add sodium hypochlorite, pandan leaf extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin and potassium dihydrogen phosphate in sequence, with an interval of 7 minutes between each component. When adding the last component, add the remaining deionized water and continue stirring for 20 minutes.

[0111] S3. Immersion Maintenance: Immerse the roots in the maintenance solution. Specifically, insert two electrodes into the maintenance solution and position them on both sides of the roots of the Anthurium seedling. The closest distance between any electrode and the Anthurium root should be 5cm. Repeat this every 24 hours for a total of 5 times. Each time, start the power supply and increase the voltage to the target value of 1.2V at a rate of 0.2V / s, then maintain the output current at 0.15mA for 15 minutes. After that, decrease the voltage at a rate of 0.2V / s and turn off the power supply.

[0112] During the soaking process, air was used as an air source to aerate the curing solution for 5 minutes every 17 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc was 0.12 mm, and the distance between adjacent aeration holes was 2.5 mm. The ratio of the volume of aeration per cycle to the volume of curing solution was m. 3 The concentration of the curing solution is 0.07, the temperature of the curing solution during the treatment process is 25℃, and the light intensity is 1700 lux. The energizing and aeration of the curing solution should not overlap in time. Specifically, the energizing and aeration of the curing solution should not be performed at the same time. The two should be separated. Specifically, the energizing of the curing solution should be started at least 2 minutes after the aeration ends, or the aeration should be started after the energizing ends.

[0113] S4. Spray castor oil evenly onto the base of the anthurium, at a rate of 1.5 mL per plant.

[0114] S5. Place the Anthurium seedlings in a dark environment for 16 hours at a temperature of 25°C and a relative humidity of 75%.

[0115] Comparative Example 3

[0116] The difference from Example 3 lies in the removal of the pandan leaf extract and its preparation process from the maintenance solution:

[0117] This invention provides a method for promoting root growth and maintaining Anthurium roots before transplanting, comprising the following steps:

[0118] S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove rotten roots, dead roots and other undesirable roots for later use;

[0119] S2. Preparation of maintenance solution: The maintenance solution includes sodium hypochlorite 0.2 g / mL, anthurium flower extract 0.25 g / mL, calcium nitrate 1.0 g / mL, sodium silicate 0.5 g / mL, lanthanum nitrate 0.08 g / mL, chitin 2.0 g / mL, and potassium dihydrogen phosphate 0.7 g / mL, dissolved in deionized water.

[0120] The extraction steps for Anthurium flower extract are as follows:

[0121] S211. Collect mature Anthurium flowers, remove damaged parts, wash with deionized water and drain, crush the flowers to a particle size of 2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities.

[0122] S212. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 700W / L.

[0123] The preparation steps for the maintenance solution include:

[0124] S21. Heat 80% deionized water to 43°C and stir continuously at a stirring speed of 110 rpm.

[0125] S22. Add sodium hypochlorite, anthurium flower extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin and potassium dihydrogen phosphate in sequence, with an interval of 7 minutes between each component. When adding the last component, add the remaining deionized water and continue stirring for 20 minutes.

[0126] S3. Immersion Maintenance: Immerse the roots in the maintenance solution. Specifically, insert two electrodes into the maintenance solution and position them on both sides of the roots of the Anthurium seedling. The closest distance between any electrode and the Anthurium root should be 5cm. Repeat this every 24 hours for a total of 5 times. Each time, start the power supply and increase the voltage to the target value of 1.2V at a rate of 0.2V / s, then maintain the output current at 0.15mA for 15 minutes. After that, decrease the voltage at a rate of 0.2V / s and turn off the power supply.

[0127] During the soaking process, air was used as an air source to aerate the curing solution for 5 minutes every 17 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc was 0.12 mm, and the distance between adjacent aeration holes was 2.5 mm. The ratio of the volume of aeration per cycle to the volume of curing solution was m. 3 The concentration of the curing solution is 0.07, the temperature of the curing solution during the treatment process is 25℃, and the light intensity is 1700 lux. The energizing and aeration of the curing solution should not overlap in time. Specifically, the energizing and aeration of the curing solution should not be performed at the same time. The two should be separated. Specifically, the energizing of the curing solution should be started at least 2 minutes after the aeration ends, or the aeration should be started after the energizing ends.

[0128] S4. Spray castor oil evenly onto the base of the anthurium, at a rate of 1.5 mL per plant.

[0129] S5. Place the Anthurium seedlings in a dark environment for 16 hours at a temperature of 25°C and a relative humidity of 75%.

[0130] Comparative Example 4

[0131] The difference from Example 3 is that a voltage of 2V and a current of 0.3mA are introduced into the curing solution:

[0132] This invention provides a method for promoting root growth and maintaining Anthurium roots before transplanting, comprising the following steps:

[0133] S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove rotten roots, dead roots and other undesirable roots for later use;

[0134] S2. Preparation of maintenance solution: The maintenance solution includes sodium hypochlorite 0.2 g / mL, anthurium flower extract 0.25 g / mL, pandan leaf extract 7 g / mL, calcium nitrate 1.0 g / mL, sodium silicate 0.5 g / mL, lanthanum nitrate 0.08 g / mL, chitin 2.0 g / mL, and potassium dihydrogen phosphate 0.7 g / mL.

[0135] The extraction steps for Anthurium flower extract are as follows:

[0136] S211. Collect mature Anthurium flowers, remove damaged parts, wash with deionized water and drain, crush the flowers to a particle size of 2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities.

[0137] S212. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 700W / L.

[0138] The extraction steps for pandan leaf extract are as follows:

[0139] S221. Wash the pandan leaves with deionized water and drain them. Crush the pandan leaves to a particle size of 2 mm and then extract the juice. Collect the juice and filter it through a 0.8 μm microporous membrane to remove impurities.

[0140] S222. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity of 70 μW / cm². 2 The sample was treated for 15 minutes under the specified conditions, supplemented by 35KHz ultrasonic treatment, with the power density controlled at 700W / L.

[0141] The preparation steps for the maintenance solution include:

[0142] S21. Heat 80% deionized water to 43°C and stir continuously at a stirring speed of 110 rpm.

[0143] S22. Add sodium hypochlorite, anthurium flower extract, pandan leaf extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin and potassium dihydrogen phosphate in sequence, with an interval of 7 minutes between each component. When adding the last component, add the remaining deionized water and continue stirring for 20 minutes.

[0144] S3. Immersion Maintenance: Immerse the roots in the maintenance solution. Specifically, insert two electrodes into the maintenance solution and position them on both sides of the roots of the Anthurium seedling. The closest distance between any electrode and the Anthurium root should be 5cm. Repeat this every 24 hours for a total of 5 times. Each time, start the power supply and increase the voltage to the target value of 2V at a rate of 0.2V / s, then maintain the output current at 0.3mA for 15 minutes. After that, decrease the voltage at a rate of 0.2V / s and turn off the power supply.

[0145] During the soaking process, air was used as an air source to aerate the curing solution for 5 minutes every 17 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc was 0.12 mm, and the distance between adjacent aeration holes was 2.5 mm. The ratio of the volume of aeration per cycle to the volume of curing solution was m. 3 The concentration of the curing solution is 0.07, the temperature of the curing solution during the treatment process is 25℃, and the light intensity is 1700 lux. The energizing and aeration of the curing solution should not overlap in time. Specifically, the energizing and aeration of the curing solution should not be performed at the same time. The two should be separated. Specifically, the energizing of the curing solution should be started at least 2 minutes after the aeration ends, or the aeration should be started after the energizing ends.

[0146] S4. Spray castor oil evenly onto the base of the anthurium, at a rate of 1.5 mL per plant.

[0147] S5. Place the Anthurium seedlings in a dark environment for 16 hours at a temperature of 25°C and a relative humidity of 75%.

[0148] Test results

[0149] 1. Compression test:

[0150] Randomly select Anthurium seedlings from Examples 1 to 3 and Comparative Examples 1 to 4 after cultivation. Each example or comparative example constitutes a group, with 10 seedlings selected in each group. After cutting the adventitious roots from the connecting stem, place the adventitious roots flat on a platform, utilizing an area of ​​9mm². 2 A planar indenter was placed in the middle of the adventitious root, and the pressure value at which the root was fractured was recorded. The average value of all roots fractured in each group was calculated, and the results are shown in Table 1.

[0151] 2. Bending resistance test:

[0152] Anthurium seedlings that had been cultivated in Examples 1 to 3 and Comparative Examples 1 to 4 were randomly selected. Each example or comparative example constituted a group, and 10 seedlings were selected in each group. Adventitious roots with a length of ≥4CM were cut off from the connecting stem. The middle 2cm of the adventitious root was cut off, and the two ends were fixed or clamped about 5mm apart and folded inward. The angle at which the root was folded was recorded. The folding angle was the angle between the folding point and the line connecting the two ends. The average value of all roots folded in each group was calculated. The results are shown in Table 1.

[0153] Table 1

[0154]

[0155]

[0156] Note: The rate of decline is rounded to the nearest whole number.

[0157] 3. Self-healing and root-promoting experiments:

[0158] Anthurium seedlings from Examples 1 to 3 and Comparative Examples 1 to 4, after cultivation, were randomly selected. Each example or comparative example constituted a group, with 20 seedlings selected in each group. Four roots from each seedling, representing the largest, second largest, smallest, and second smallest root diameters, were drilled with holes centered on the roots at their midpoints. The hole diameter was one-third of the root diameter at that point. The seedlings were transplanted into the soil and planted for 20 days. The adventitious root growth was then observed. The results are shown in Table 2.

[0159] Evaluation criteria:

[0160] Wound healing rate = Number of roots with completely healed wounds in a single group / Number of all perforated roots in a single group before transplantation × 100%;

[0161] Average number of new roots: (Number of new roots in all anthuriums in a single group / Number of anthuriums in a single group) × 100%;

[0162] Incidence of root rot = Number of rotten roots at the wound site in a single group / Number of all perforated roots in the group before transplantation × 100%;

[0163] Table 2

[0164] Processing group Wound healing rate (%) Average of new roots (number of roots) Incidence of root rot (%) Example 1 97.5％ 11.2 2.5％ Example 2 100％ 12.5 0 Example 3 100％ 13.1 0 Comparative Example 1 40％ 4.5 60％ Comparative Example 2 45％ 5.5 55％ Comparative Example 3 55％ 6.2 45％ Comparative Example 4 35％ 4.1 65％

[0165] Because during the soaking and maintenance process of the anthurium's roots, introducing appropriate voltage and current into the maintenance solution can promote the absorption of calcium ions (Ca) while avoiding electrical damage. 2+ Sodium ions (Na) + ) and lanthanum ion (La 3+ Dissociation and migration of ) . Anthurium flower polysaccharide chelation of Ca 2+ / La 3+ Forming an easily absorbed complex, linoleic acid from pandanus leaf activates Na+. + / K + -ATPase, together with the other two, activates the opening of ion channels in root cell membranes, enhancing ATP synthase activity and improving root ion absorption efficiency. This process, through physical stimulation and chemical synthesis, increases calcium deposition in the cell wall and enhances lignin synthase activity, while also strengthening cell wall cross-linking. Simultaneously, the anthurium flower glucose derivative and the variegated leaf vitamin K3 synergistically activate the glycolysis pathway, promoting root cell energy reserves. Therefore, Examples 1 to 3, through the synergistic effect of the conditioning solution and electrical stimulation, significantly improve root compressive strength, bending resistance, wound healing rate, and new root germination ability. Among these, Example 3, with its optimal conditioning solution ratio and treatment process, exhibits the best overall effect.

[0166] Comparative Example 1 (Removal of Electrical Stimulation): The absence of an electric field leads to decreased ion migration efficiency, insufficient opening of ion channels in root cell membranes, and Ca2+. 2+ La 3+ La 3+When nutrient absorption is hindered, ATP synthesis decreases. Insufficient cell wall thickening leads to a decrease in root cracking pressure, reduced bending resistance, decreased wound healing rate, and increased root rot rate, weakening the growth capacity of new roots. It can be seen that directly injecting appropriate current and voltage into the nutrient solution and directly contacting the roots of the anthurium has the greatest impact on the nutrient solution's effectiveness.

[0167] Comparative Example 2 (Extract from Anthurium Flowers Removed): The lack of polysaccharides and polyphenols in Anthurium flowers led to calcium deficiency. 2+ / La 3+ Decreased chelation capacity, reduced calcium deposition in cell walls, and decreased lignin synthase activity. The synergistic effect of sodium silicate and calcium nitrate is disrupted, preventing the formation of a silica gel protective film on the root surface and resulting in loss of physical barrier function. This ultimately leads to decreased fracturing pressure, decreased fracture angle, and increased root rot rate. Furthermore, due to the lack of synergistic activation of the glycolysis pathway by anthurium glucose derivatives and variegated leaf vitamin K3, root cell energy reserves cannot be promoted. The dual deficiency of both physical and chemical defenses in the roots inhibits meristematic tissue activity, directly limiting the germination of new roots.

[0168] Comparative Example 3 (removal of pandan leaf extract): The lack of linoleic acid in pandan leaves led to Na+ deficiency. + / K + Decreased ATPase activity hinders transmembrane ion transport and reduces cell wall mechanical strength. Simultaneously, the absence of 2-acetyl-1-pyrroline decreases cell membrane potential stability and weakens the synergistic effect of electrical stimulation. This leads to decreased fracturing pressure and fracture angle. Furthermore, it results in a double decrease in root cell energy metabolism (ATP synthesis) and ion uptake efficiency, leading to insufficient supply of substances and energy required for new root germination and a reduction in the number of new roots.

[0169] Comparative Example 4 (Increased Voltage and Current): High voltage causes excessive electrolysis of the nutrient solution, producing excessive reactive oxygen species and damaging the cell membrane integrity in the root hair zone; strong current interferes with ion channel homeostasis and inhibits Ca2+. 2+ Orderly deposition leads to abnormal cell wall development. This results in decreased fracturing pressure, increased root rot rate, and decreased wound healing rate. Based on the comparative results of Comparative Example 1 and Comparative Example 4, it can be shown that inappropriate electrical stimulation is more detrimental than no stimulation.

[0170] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for promoting root growth and maintaining Anthurium roots before transplanting, characterized in that: Includes the following steps, S1. Pretreatment: Select Anthurium seedlings that meet the transplanting standards and remove any unhealthy roots; S2. Preparation of the maintenance solution: The maintenance solution comprises, by mass-volume ratio g / mL, 0.1-0.3g sodium hypochlorite, 0.2-0.3g anthurium flower extract, 5-10g pandanus leaf extract, 0.8-1.2g calcium nitrate, 0.3-0.6g sodium silicate, 0.05-0.1g lanthanum nitrate, 1.5-2.5g chitosan, and 0.5-1.0g potassium dihydrogen phosphate dissolved in deionized water; S3. Immersion maintenance: Immerse the roots in the maintenance solution and apply direct current to the maintenance solution. The voltage is 1.0-1.5V and the current is 0.1-0.2mA. Each time, it is 10-20 minutes. Repeat this every 22-26 hours for a total of 4-6 times.

2. The method for promoting root growth and maintaining Anthurium roots before transplanting according to claim 1, characterized in that: During the soaking process in step S3, the curing solution is aerated with air for 5 minutes every 15-20 minutes using a microporous aeration disc. The diameter of the aeration holes in the microporous aeration disc is 0.1-0.15 mm, the spacing between adjacent aeration holes is 2-3 mm, and the ratio of single aeration volume to curing solution volume is m. 3 / L is 0.05-0.

1.

3. The method for promoting root growth and maintaining Anthurium roots before transplanting according to claim 1, characterized in that: The extraction steps for the anthurium flower extract are as follows: S211. Collect mature Anthurium flowers, remove damaged parts, wash with deionized water and drain. Crush the flowers to a particle size ≤2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities. S212. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity ≥70 μW / cm². 2 Under these conditions, the sample is treated for 10-15 minutes, supplemented with 35KHz ultrasonic treatment, and the power density is controlled at 50-100W / L.

4. The method for promoting root growth and maintaining Anthurium before transplanting according to claim 1, characterized in that: The extraction steps for the pandan leaf extract are as follows: S221. Wash the pandan leaves with deionized water and drain them. Crush the pandan leaves to a particle size ≤2mm and then juice them. Collect the juice and filter it through a 0.8μm microporous membrane to remove impurities. S222. The juice is sterilized with ultraviolet light at a wavelength of 253.7 nm and an irradiation intensity ≥70 μW / cm². 2 Under these conditions, the sample is treated for 10-15 minutes, supplemented with 35KHz ultrasonic treatment, and the power density is controlled at 50-100W / L.

5. The method for promoting root growth and maintaining Anthurium roots before transplanting according to claim 1, characterized in that: The preparation of the maintenance solution in step S2 includes the following steps: S21. Heat 80% deionized water to 40-45℃ and stir continuously at a speed of 100-120 rpm. S22. Add sodium hypochlorite, anthurium flower extract, pandan leaf extract, calcium nitrate, sodium silicate, lanthanum nitrate, chitin, and potassium dihydrogen phosphate in sequence, with an interval of 5-10 minutes between each component. After adding the last component, add the remaining deionized water and continue stirring for more than 20 minutes.

6. The method for promoting root growth and maintaining Anthurium roots before transplanting according to claim 1, characterized in that: In step S3, insert two electrodes into the nutrient solution and position them on either side of the roots of the Anthurium seedling. The closest distance between either electrode and the roots of the Anthurium should be at least 5 cm. Turn on the power supply and increase the voltage to the target value at a rate of 0.2 V / s. When the preset value is reached, decrease the voltage at a rate of 0.2 V / s and turn off the power supply.

7. The method for promoting root growth and maintaining Anthurium before transplanting according to claim 1, characterized in that: In step S3, the temperature of the curing solution should be controlled at 20-30℃, and the light intensity should be maintained at 1500-2000 lux.

8. The method for promoting root growth and maintaining Anthurium roots before transplanting according to claim 1, characterized in that: After completing step S3, proceed to step S4: spray castor oil evenly onto the roots of the anthurium, using 1-2 mL per plant.

9. The method for promoting root growth and maintaining Anthurium roots before transplanting according to claim 1, characterized in that: After completing step S4, proceed to step S5: place the Anthurium seedlings in a dark environment for 12-18 hours at a temperature of 22-25℃ and a relative humidity of 70%-80%.

10. The method for promoting root growth and maintaining Anthurium roots before transplanting according to claim 1, characterized in that: The term "unhealthy roots" includes rotten roots and dead roots.

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