Anthurium andraeanum tissue culture seedling propagation and inoculation device

Abstract

The invention discloses an anthurium andraeanum tissue culture seedling propagation and inoculation device which comprises a layered goods shelf, and light sources are arranged at the tops of compartments of the goods shelf. The core of the unit is an inoculation box, and the bottom of the front end of the inoculation box is provided with a drainage pipe and a drainage valve. A partition frame is arranged in the inoculation box, a plurality of slots are formed in the partition frame, plug bushes are detachably arranged in the slots, upper and lower openings are formed in the plug bushes, connecting rods are arranged in the plug bushes to fix a plug box, a first through hole is formed in the bottom of the plug box, and limiting foam is placed in the first through hole. The top of the vertical rod is provided with an adjusting box, the bottom of the box is provided with a second through hole for light transmission, and a shading part can be placed in the box to adjust the light transmission intensity. An illumination intensity sensor is arranged between the plug bush and the second through hole, and an illumination intensity display assembly is arranged at the front end of the compartment and electrically connected with the sensor. In addition, a stirring assembly is further arranged in the inoculation box and used for stirring liquid in the box. According to the device, the inoculation posture of the tender tissue can be ensured, the pH value of a culture solution can be easily maintained, and proper illumination can be easily provided for each tender tissue, so that the survival rate and the superior product rate of propagation inoculation are improved.

Description

Technical Field

[0001] The invention relates to the technical field of anthurium andraeanum propagation, and in particular to an anthurium andraeanum tissue culture seedling propagation inoculation device. Background Art

[0002] Anthurium, scientifically known as Anthurium, is a perennial evergreen herb of the genus Anthurium in the Araceae family. Native to the tropical rainforests of South America, Anthurium is beloved for its unique flower shape and vibrant colors. Anthurium propagation techniques primarily include seed propagation, division, and tissue culture. Tissue culture, which involves cultivating young Anthurium tissue under sterile conditions, can rapidly and massively produce plants with identical genetic characteristics to the parent plant. This method is particularly suitable for the rapid propagation of superior varieties, not only enabling the cultivation of superior varieties but also meeting market demand for Anthurium seedlings.

[0003] In tissue culture propagation, key techniques include the extraction of young tissue, the preparation of culture medium, the inoculation position of the young tissue in the culture medium, temperature control of the culture environment, and light control. When properly controlled, these key techniques can not only improve the survival rate of young tissue inoculation, but also increase its growth rate and quality rate.

[0004] After many tests and verifications, the existing tissue culture seedlings are generally selected to select healthy adventitious buds larger than 2 cm and inoculate them into the culture medium during the inoculation process. The pH value of the culture medium is maintained between 5.5 and 5.8. The growth temperature is 24 to 28 ° C, the light intensity is appropriate, and the light duration is appropriate. Healthy anthurium tissue culture seedlings can be obtained within 40 to 60 days. Among the above conditions, the control of growth temperature is relatively easy to achieve. Currently, there are relatively mature and low-cost greenhouse culture rooms or sheds used in various industries. Although the other conditions have relatively mature application methods in individual cases, they have not yet been effectively combined to provide a suitable device for the expansion and inoculation of anthurium tissue culture seedlings.

[0005] Therefore, in order to meet the above-mentioned propagation and inoculation conditions, it is necessary to design a device that can achieve the above-mentioned propagation and inoculation conditions, which can ensure the inoculation posture of young tissues, can easily maintain the pH value of the culture medium stable, and can provide suitable light for each young tissue, so as to reduce the difficulty of propagation and inoculation and improve the survival rate and quality rate of propagation and inoculation. Summary of the Invention

[0006] The purpose of the present invention is to provide an anthurium andraeanum tissue culture seedling propagation and inoculation device to solve the problems described in the background technology.

[0007] The technical solution of the present invention is achieved in this way:

[0008] Anthurium andraeanum tissue culture seedling propagation inoculation device, including a layered shelf. The layered shelf includes several compartments separated up and down. There are several light sources at the top of the compartment. An inoculation unit is placed in the compartment under the light source. The inoculation unit includes an inoculation box with an open upper end. A drain pipe is provided at the bottom of the front end of the inoculation box. A drain valve is provided on the drain pipe. A partition shelf is provided in the inoculation box, which is spaced from the bottom of the inoculation box. Several slots are provided inside the partition shelf. A socket is detachably and fixably inserted into the slot from top to bottom. The socket is an open structure at both the upper and lower ends. A connecting rod and an upper-end open socket box are further provided inside the socket. The socket box is fixedly connected to the socket through the connecting rod. A first through hole is opened at the bottom of the socket box. A circular columnar limiting foam is placed inside the socket box. A vertical rod is also fixedly provided at the top of the socket. An adjusting box with an open upper end is further fixedly provided at the top of the vertical rod. The adjusting box is used to block directly above the socket. A second through hole for providing a light transmission path for the socket is opened at the bottom of the adjusting box. A light-shielding component for adjusting the light transmission intensity of the second through hole is detachably placed inside the adjusting box. A light intensity sensor is also provided between the socket and the second through hole. A light intensity display component is further provided at the front end of the compartment. The light intensity sensor is electrically connected to the light intensity display component. A stirring component for the liquid inside the inoculation box is also provided inside the inoculation box.

[0009] When using the above solution, place the layered shelf in a greenhouse or shed with temperature control. Then pour the culture solution with adjusted pH value into the inoculation box so that the culture solution submerges the limiting foam. Then insert the adventitious buds obtained into the limiting foam, keeping the adventitious buds in a vertical posture. The bottom of the adventitious bud passes through the limiting foam downward and then passes through the first through hole downward. The top of the adventitious bud passes through the limiting foam upward. The setting of the limiting foam can not only absorb the culture solution, but also softly clamp the adventitious buds, ensuring the inoculation posture of the adventitious buds. Insert the sockets with inoculated adventitious buds into the slots of the partition shelf one by one, and put the partition shelf into the inoculation box uniformly. At this point, all the adventitious buds in the partition shelf are immersed in the culture solution of the inoculation box. Place the inoculation boxes in the compartments of the layered shelf in sequence, connect the light intensity sensor and the light intensity display component, and then turn on the light source. According to the value feedback by the light intensity display component, adjust the light-shielding component to adjust the light intensity passing through the second through hole, so that each adventitious bud in each socket is within an appropriate light intensity range.

[0010] Due to the overlapping of the irradiation areas of multiple light sources in the same layer of compartments of the layered shelf, plus the superposition of the light intensity of the greenhouse and the shed itself, and the factor of different placement positions of the layered shelf, the light intensity received by each adventitious bud in the same inoculation box is different. Under this precondition, it is unrealistic to provide a separate light source for the top of each adventitious bud. By setting the light-shielding component in cooperation with the light intensity sensor and the light intensity display component, a suitable light can be provided for each young tissue at low cost.

[0011] By providing an inoculation box to increase the volume of the culture solution, the volume of the culture solution is increased. The larger volume of culture solution has higher stability. Compared with a small amount of culture solution in a small volume container, the changes in the pH value and the changes in the nutrient components of the culture solution in the large volume container tend to be slow, providing a buffer time for the growers to discover and adjust, which is more conducive to the stable development of adventitious buds. By providing a drain pipe, a drain valve and a stirring assembly at the front end of the inoculation box, when the pH value of the culture solution is monitored to change, the growers can conveniently release part of the culture solution through the drain valve at the front end of the layered shelf to facilitate the discharge of the liquid. At the same time, after adding new culture solution, the stirring assembly can make the newly added culture solution quickly and evenly distributed throughout the inoculation box. Through the operation of adding one row at a time, the pH value of the culture solution in the inoculation box can be adjusted quickly and conveniently on the spot.

[0012] The device can ensure the inoculation posture of young and tender tissues, can easily maintain the pH value of the culture solution stable, and can provide suitable light for each young and tender tissue, so as to reduce the difficulty of propagation inoculation and improve the survival rate and quality rate of propagation inoculation.

[0013] A further technical solution is that the partition frame includes a first frame and a first grid arranged inside the first frame, the first grid has a plurality of mesh holes, and the mesh holes constitute the slots. The partition frame also includes a plurality of support rods fixed to the bottom of the first frame, and the bottom of the support rods is against the bottom wall of the inoculation box to support the first frame and separate it from the bottom of the inoculation box.

[0014] When the above solution is used, the partition frame is formed by arranging the first frame, the first grid and the support rods, which has the advantages of simple structure and low cost, and is easy to disassemble and assemble, and simple to clean and maintain.

[0015] A further technical solution is that the top of the sleeve further extends outward to form a limiting frame, the sleeve is slidably inserted into the slot, and the sleeve is placed on the first grid through the limiting frame.

[0016] When the above solution is used, the design of the limit frame has the advantages of simple structure and low cost, and is easy to assemble and disassemble.

[0017] A further technical solution is that the stirring assembly includes a sleeve fixed on the inner wall of the inoculation box, and a vertically arranged rotating shaft is rotatably installed in the sleeve. The rotating axis of the rotating shaft is the central axis of the rotating shaft. A turning handle is fixed on the top of the rotating shaft, and a long plate is fixed horizontally on the bottom of the rotating shaft.

[0018] When using the above solution, the incubator grasps the handle and rotates it back and forth, which drives the shaft to rotate, and the shaft drives the long plate to swing back and forth, so that the new and old culture solutions in the inoculation box are evenly mixed. This design is not only simple in structure and low in cost, but also easy to operate.

[0019] A further technical solution is that the light-shielding component includes a placing net and a light-shielding net. The placing net is a net with a rigid structure, and the light-shielding net is a net with a flexible structure. The placing net is placed above the second through-hole, and the edge of the placing net is placed on the bottom of the adjusting box surrounding the second through-hole. The light-shielding net is placed above the placing net.

[0020] When using the above solution, the placing net is used to hold the light-shielding net. The light-shielding net with a flexible structure is not only easy to cut, but also easy to put in and take out, reducing the difficulty of adjusting the light-shielding net. When it is necessary to adjust the light intensity, the covering layer number of the light-shielding net can be adjusted or the light-shielding net with different apertures can be used.

[0021] A further technical solution is that an installation rod is also fixedly provided at the upper end of the connecting rod, and the light intensity sensor is installed on the installation rod.

[0022] When using the above solution, by setting the installation rod, it is convenient to install the light intensity sensor.

[0023] A further technical solution is that the light intensity display component includes a circuit board provided at the front end of the compartment. A plurality of digital display boards are installed on the circuit board, and a controller is also installed on the circuit board. The controller is electrically connected to the circuit board. The number of digital display boards is the same as the number of light intensity sensors, and the light intensity sensors are electrically connected to the digital display boards through the controller.

[0024] When using the above solution, the circuit board is used to install and connect the digital display boards and the controller, and it is also convenient to install a plurality of digital display boards orderly at the front end of the compartment. The light intensity sensors are connected to the digital display boards through the controller, which is convenient to adjust the voltage and current values and use the controller to install a suitable warning device, reducing the monitoring intensity of the cultivation personnel.

[0025] A further technical solution is that the inoculation box is a cuboid box, the first frame is a rectangular frame, the slot of the first wire rack is a rectangular slot, the socket is a sleeve in the shape of a ring rectangle column, the number of vertical rods is four and they are respectively arranged at the four corners of the socket, the adjusting box is a cuboid box, and the tops of the vertical rods are respectively connected to the four corners of the bottom of the adjusting box. The second through-hole is a rectangular hole.

[0026] When using the above solution, the structures of the cuboid-shaped and rectangular-shaped components can achieve a high space utilization rate.

[0027] A further technical solution is that an opening is provided at the front end of the adjusting box, and a bevel platform is also provided at the front-end opening of the adjusting box. The bevel platform slopes downward gradually from the outside of the adjusting box to the inside of the adjusting box.

[0028] When using the above solution, by setting the bevel platform, the front end of the light-shielding net can be lifted through the bevel platform, which is convenient to put in and take out the light-shielding net from the front end.

[0029] The beneficial effects of the present invention are as follows:

[0030] 1. Precise light adjustment: Through the cooperation of a light intensity sensor and a display component with a light-shielding component, it can provide suitable light for each young tissue at a low cost, effectively addressing the problem of uneven light intensity in greenhouses or sheds.

[0031] 2. High stability of the culture solution: By setting up an inoculation box to increase the volume of the culture solution, the stability of the culture solution is improved, making the changes in pH value and nutrient components tend to be slow, providing a buffer time for cultivation personnel to adjust and discover, which is beneficial to the stable development of adventitious buds.

[0032] 3. Convenient pH value adjustment: The drain pipe, drain valve and stirring component set at the front end of the inoculation box make the adjustment of the pH value of the culture solution convenient and fast. The pH value of the culture solution in the inoculation box can be adjusted in place through the operation of draining and adding in turn.

[0033] 4. Guarantee of inoculation posture: The design of the limit foam can not only absorb the culture solution, but also softly clamp the adventitious buds, ensuring the inoculation posture of the adventitious buds, which is beneficial to their growth.

[0034] 5. Simple structure and low cost: The overall structure of this device is simple and easy to implement, with low cost, improving the practicability and economy of the whole device.

[0035] 6. Convenient adjustment of the shading net: The shelving net is used to hold the shading net. The flexible structure of the shading net is convenient for cutting, putting in and taking out, reducing the adjustment difficulty. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 is the overall schematic diagram of the present invention;

[0037] Figure 2 is the enlarged structural view of the inoculation unit;

[0038] Figure 3 is the three-dimensional view of the socket and each component;

[0039] Figure 4 is the three-dimensional view of the socket and each component from another perspective;

[0040] Figure 5 is the top view matching diagram of the inoculation box and the partition;

[0041] Figure 6 is the top view of the socket and each component.

[0042] In the figure, 1 is a layered shelf, 2 is a compartment, 3 is a light source, 4 is a digital display board, 5 is a circuit board, 6 is an inoculation unit, 7 is an inoculation box, 8 is a support rod, 9 is a first frame, 10 is a long board, 11 is a rotating shaft, 12 is a shaft sleeve, 13 is a turning handle, 14 is a socket, 15 is a limiting frame, 16 is a first wire mesh rack, 17 is a connecting rod, 18 is a plug-in box, 19 is a first through hole, 20 is a limiting foam, 21 is a tender tissue, 22 is a mounting rod, 23 is a vertical rod, 24 is an adjusting box, 25 is a second through hole, 26 is a shelving net, 27 is a light-shielding net, 28 is an inclined platform, 29 is a slot, 30 is a drain pipe, 31 is a drain valve. Detailed implementation manner

[0043] In order to better understand the technical content of the present invention, specific embodiments are provided below, and the present invention will be further described in conjunction with the accompanying drawings.

[0044] See Figures 1 to 6 , a propagation inoculation device for Anthurium andraeanum tissue culture seedlings, including a layered shelf 1, and the layered shelf 1 includes a plurality of compartments 2 separated up and down.

[0045] It should be particularly noted that the layered shelf 1 can be made of shelves of various materials, and its main structure is a compartment 2 with a plurality of cuboid spaces from top to bottom for placing the inoculation unit 6.

[0046] A plurality of light sources 3 are provided at the top of the compartment 2, and the light sources 3 can be LED light sources 3, preferably strip-shaped light sources 3.

[0047] The inoculation unit 6 is placed in the compartment 2 under the light source 3. The inoculation unit 6 includes an inoculation box 7 with an open upper end. Preferably, the inoculation box 7 is a cuboid box. A drain pipe 30 is provided at the bottom of the front end of the inoculation box 7, and a drain valve 31 is provided on the drain pipe 30. A partition shelf is provided in the inoculation box 7 at a distance from the bottom of the inoculation box 7, and a plurality of slots 29 are provided inside the partition shelf.

[0048] Specifically, the partition shelf includes a first frame 9 and a first wire mesh rack 16 provided inside the first frame 9. Preferably, the first frame 9 is a rectangular frame. The first wire mesh rack 16 has a plurality of mesh holes, and the mesh holes form the slots 29. Preferably, the slots 29 of the first wire mesh rack 16 are rectangular slots 29, and a plurality of rectangular slots 29 are arranged in a matrix. The partition shelf also includes four support rods 8 respectively fixed at the four corners of the bottom of the first frame 9, and the bottom of the support rods 8 abuts against the inner bottom wall of the inoculation box 7 to support the first frame 9 to be separated from the bottom of the inoculation box 7.

[0049] A socket 14 is detachably and fixably inserted into the slot 29 from top to bottom, and the socket 14 is an open structure at both the top and the bottom.

[0050] Specifically, the socket 14 is a sleeve in the shape of a ring rectangle column. The top of the socket 14 also extends outward to form a limit frame 15 in the shape of a ring rectangle. The socket 14 is slidably inserted into the slot 29, and the socket 14 is placed on the first grid 16 through the limit frame 15.

[0051] Inside the socket 14, there are also two connecting rods 17 and a plug-in box 18 with an open upper end. The outer wall of the plug-in box 18 is fixedly connected to the socket 14 through the connecting rod 17. A first through hole 19 is opened at the bottom of the plug-in box 18. A ring-shaped columnar limit foam 20 is placed inside the plug-in box 18. The limit foam 20 is made of foam with a porous structure, which has water absorption while also having good flexibility and elasticity to wrap and limit the tender tissue 21 (adventitious bud).

[0052] At the top of the socket 14, there is also a vertical rod 23 fixedly installed. The number of the vertical rods 23 is four and they are respectively arranged at the four corners of the socket 14. At the top of the vertical rod 23, there is also a regulating box 24 with an open upper end. The regulating box 24 is used to block directly above the socket 14. The regulating box 24 is a cuboid box. The tops of the vertical rods 23 are respectively connected to the four corners at the bottom of the regulating box 24. A second through hole 25 for providing a light transmission path for the socket 14 is opened at the bottom of the regulating box 24. The second through hole 25 is a rectangular hole. A light-shielding component for adjusting the light transmission intensity of the second through hole 25 is detachably placed inside the regulating box 24.

[0053] Specifically, the light-shielding component includes a shelf net 26 and a light-shielding net 27. The shelf net 26 is a net with a rigid structure made of PP plastic. The light-shielding net 27 is a net with a flexible structure made of polyethylene plastic. The shelf net 26 is placed above the second through hole 25, and the edge of the shelf net 26 is placed on the bottom of the regulating box 24 outside the periphery of the second through hole 25. The light-shielding net 27 is placed above the shelf net 26.

[0054] Preferably, the front end of the regulating box 24 is provided with an opening, and a bevel platform 28 is also provided at the opening at the front end of the regulating box 24. The bevel platform 28 slopes downward gradually from the outside of the regulating box 24 to the inside of the regulating box 24.

[0055] A light intensity sensor is also provided between the socket 14 and the second through hole 25.

[0056] Specifically, at the upper end of the connecting rod 17, there is also a mounting rod 22 fixedly installed. The light intensity sensor is installed on the mounting rod 22, and the sensing direction of the light intensity sensor is upward and aligned with the second through hole 25.

[0057] At the front end of the compartment 2, there is also a light intensity display component. The light intensity sensor is electrically connected to the light intensity display component.

[0058] Specifically, the light intensity display component includes a circuit board 5 provided at the front end of the compartment 2. The circuit board 5 can be a customized PCB circuit board 5 or a basic breadboard. A number of digital display boards 4 are installed on the circuit board 5. A controller is also installed on the circuit board 5, and the controller is electrically connected to the circuit board 5. The number of digital display boards 4 is the same as the number of light intensity sensors. The light intensity sensors are electrically connected to the digital display boards 4 through the controller. The controller uses an STM32 single-chip microcomputer or a SMART200 PLC controller and is connected to electrical components such as a buzzer or a warning light for giving a warning when the light intensity is abnormal.

[0059] A stirring component for the liquid in the inoculation box 7 is also provided in the inoculation box 7.

[0060] Specifically, the stirring component includes a bushing 12 fixedly arranged on the inner side wall of the inoculation box 7. A vertically arranged rotating shaft 11 is rotatably installed in the bushing 12. The rotation axis of the rotating shaft 11 is the central axis of the rotating shaft 11. A rotating handle 13 is fixedly arranged at the top of the rotating shaft 11, and a long plate 10 is fixedly horizontally arranged at the bottom of the rotating shaft 11.

[0061] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A propagation inoculation device for Anthurium andraeanum tissue culture seedlings, comprising a layered shelf, the layered shelf including several compartments separated up and down, and several light sources being provided at the top of the compartments, characterized in that: Inside the compartment, there is an inoculation unit placed under the light source. The inoculation unit includes an inoculation box with an open top. At the bottom of the front end of the inoculation box, there is a drain pipe, and a drain valve is provided on the drain pipe. Inside the inoculation box, there is a shelf spaced from the bottom of the inoculation box. Inside the shelf, there are several slots. A sleeve is detachably and fixably inserted into the slots from top to bottom. The sleeve is an open structure at both the top and bottom. Inside the sleeve, there is also a connecting rod and a plug-in box with an open top. The plug-in box is fixedly connected to the sleeve through the connecting rod. A first through hole is opened at the bottom of the plug-in box. A ring-shaped cylindrical limit foam is placed inside the plug-in box. A vertical rod is also fixedly provided at the top of the sleeve. At the top of the vertical rod, there is also an adjustment box with an open top. The adjustment box is used to block directly above the sleeve. A second through hole for providing a light transmission path for the sleeve is opened at the bottom of the adjustment box. A light-shielding component for adjusting the light transmission intensity of the second through hole is detachably placed inside the adjustment box. A light intensity sensor is also provided between the sleeve and the second through hole. At the front end of the compartment, there is also a light intensity display component. The light intensity sensor is electrically connected to the light intensity display component. Inside the inoculation box, there is also a stirring component for the liquid inside the inoculation box.

2. The multiplication inoculation device for Anthurium andraeanum tissue culture seedlings according to claim 1, wherein: The shelf includes a first frame and a first wire mesh arranged inside the first frame. The first wire mesh has several mesh holes, and the mesh holes form the slots. The shelf also includes several support rods fixedly provided at the bottom of the first frame. The bottom of the support rods abuts against the inner bottom wall of the inoculation box to support the first frame and separate it from the bottom of the inoculation box.

3. A propagation inoculation device for Anthurium andraeanum tissue culture seedlings according to claim 2, characterized in that: The top of the sleeve also extends outward to form a limit frame. The sleeve is slidably inserted into the slot and is placed on the first wire mesh through the limit frame.

4. A propagation inoculation device for Anthurium andraeanum tissue culture seedlings according to claim 1, characterized in that: The stirring component includes a bushing fixedly provided on the inner side wall of the inoculation box. A vertically arranged rotating shaft is also rotatably installed in the bushing. The rotation axis of the rotating shaft is the central axis of the rotating shaft. A rotating handle is fixedly provided at the top of the rotating shaft, and a long plate is fixedly horizontally provided at the bottom of the rotating shaft.

5. The anthurium tissue culture seedling propagation inoculation device according to claim 1, characterized in that: The light-shielding component includes a placement mesh and a light-shielding mesh. The placement mesh is a mesh of rigid structure, and the light-shielding mesh is a mesh of flexible structure. The placement mesh is placed above the second through hole, and the edge of the placement mesh is placed on the bottom of the adjustment box around the second through hole. The light-shielding mesh is placed above the placement mesh.

6. The aroid tissue culture seedling propagation inoculation device according to claim 1, characterized in that: An installation rod is also fixedly provided at the upper end of the connecting rod. The light intensity sensor is installed on the installation rod.

7. The anthurium tissue culture seedling propagation inoculation device according to claim 6, characterized in that: The light intensity display component includes a circuit board provided at the front end of the compartment. Several digital display boards are installed on the circuit board. A controller is also installed on the circuit board. The controller is electrically connected to the circuit board. The number of digital display boards is the same as the number of light intensity sensors. The light intensity sensors are electrically connected to the digital display boards through the controller.

8. The anthurium andraeanum tissue culture seedling propagation inoculation device according to claim 3, characterized in that: The inoculation box is a rectangular box. The first frame is a rectangular frame. The slots of the first wire mesh are rectangular slots. The sleeve is a sleeve in the shape of a ring rectangle. The number of vertical rods is four and they are respectively arranged at the four corners of the sleeve. The adjustment box is a rectangular box. The top of the vertical rods are respectively connected to the four corners of the bottom of the adjustment box. The second through hole is a rectangular hole.

9. The aroid tissue culture seedling propagation inoculation device according to claim 8, characterized in that: An opening is provided at the front end of the adjustment box. A bevel platform is also provided at the front end opening of the adjustment box. The bevel platform slopes downward gradually from the outside of the adjustment box to the inside of the adjustment box.

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