Cultivation device and cultivation system for daphnia magna

Abstract

The invention discloses a daphnia magna cultivation device and system, and relates to the technical field of aquatic organism cultivation, the daphnia magna cultivation device comprises a daphnia magna cultivation box, a first screening plate and a partition plate, the bottom end of one side of the daphnia magna cultivation box is provided with a first outlet, the first outlet is provided with a first valve, and the daphnia magna cultivation box is provided with a first light source close to the first outlet; a second light source is arranged far away from the first outlet, and the first screening plate and the partition plate are detachably mounted in the daphnia culture box; when the device is used, the first outlet is in a closed state, the functions of daphnia magna screening and culture solution replacement can be achieved at the same time by controlling opening and closing of the first light source and the second light source and installing the first screening plate and the partition plate in the daphnia magna culture box, the screening efficiency is improved, a straw is not needed to suck a disturbance object, damage to the disturbance object is reduced, and the practicability is high. The daphnia magna cultivation system can also achieve the functions of feeding and culture solution filtering and recycling.

Description

Technical Field

[0001] This invention relates to the field of aquatic organism cultivation technology, and in particular to a large-scale Daphnia cultivation device and cultivation system. Background Technology

[0002] Daphnia macrocarpa is a zooplankton belonging to the genus Daphnia in the family Daphnia of the order Cladocera in the class Crustacea of ​​the phylum Arthropoda. It mainly inhabits eutrophic small bodies of water with abundant aquatic plants and is an important component of freshwater zooplankton. It feeds on algae and has characteristics such as short growth cycle, rapid reproduction and easy economic acquisition. Due to its sensitivity to toxins, it is increasingly used in environmental toxicology standard biological research and is an internationally recognized standard test organism.

[0003] In experiments on the toxicity of large Daphnia magna, juvenile Daphnia magna are often chosen as experimental organisms because they are more sensitive. It is necessary to separate the juvenile Daphnia magna from the mother. At the same time, when Daphnia magna is artificially cultivated indoors in a static culture method, its molting and food residue will change the pH and dissolved oxygen concentration of the culture medium, so frequent medium changes are required. The traditional method of separation and medium change is to transfer and separate them one by one with pipettes, which is inefficient and can easily cause damage to the organisms during the process of aspirating them one by one with pipettes. Summary of the Invention

[0004] The purpose of this invention is to provide a large-scale Daphnia cultivation device and system to solve the technical problems existing in the prior art, and to simultaneously realize the functions of large-scale Daphnia screening and culture medium replacement, thereby improving screening efficiency and reducing cell damage.

[0005] To achieve the above objectives, the present invention provides the following solution:

[0006] This invention provides a large-scale Daphnia davidii cultivation device, including a Daphnia davidii culture box for holding culture medium, mother Daphnia davidii, and juvenile Daphnia davidii. The bottom surface of the Daphnia davidii culture box has a first outlet, and a first valve is provided at the first outlet to control the opening or closing of the first outlet. A first light source is provided near the first outlet, and a second light source is provided away from the first outlet. A first sieving plate is detachably installed inside the Daphnia davidii culture box, positioned between the first and second light sources. The first sieving plate has a first sieving hole, the diameter of which only allows juvenile Daphnia davidii to pass through. A partition is detachably installed inside the Daphnia davidii culture box, positioned between the first light source and the first sieving plate, with the first outlet located on the side of the partition closer to the first light source. The partition isolates the mother Daphnia davidii and the juvenile Daphnia davidii on the side of the partition away from the first outlet.

[0007] In some embodiments, a second sieving plate is further included, which is detachably installed inside the Daphnia incubator. The second sieving plate is disposed between the first sieving plate and the partition plate. The second sieving plate has a second sieving hole with a diameter that allows the mother Daphnia and the juvenile Daphnia to pass through.

[0008] In some embodiments, the bottom surface of the Daphnia incubator is inclined downward toward the first outlet.

[0009] In some embodiments, a vibration device is installed on the outer side of the bottom surface of the daphne incubator, which is capable of causing the bottom surface of the daphne incubator to vibrate.

[0010] In some embodiments, the zeolite incubator is further provided with a first inlet, and a second valve is provided on the first inlet, the second valve being capable of controlling the opening and closing of the first inlet.

[0011] In some embodiments, a buoyancy switch is also included, which is fixed to the inner wall of the daphne incubator and located above the first outlet. When the buoyancy switch is triggered, the culture medium enters the daphne incubator through the first inlet.

[0012] The present invention also provides a large-scale Daphnia cultivation system, including a feeding device, a filtration and circulation device, and the large-scale Daphnia cultivation device described in any one of the above, wherein the feeding device is connected to the Daphnia incubator, and the filtration and circulation device is connected to the first outlet.

[0013] In some embodiments, the feeding device includes an algae culture tank, which is equipped with a culture lamp and a circulation pump.

[0014] In some embodiments, the filtration and circulation device includes a filter box and a storage tank, with the first outlet connected to one end of the filter box and the other end of the filter box connected to the storage tank, and the filter box capable of filtering impurities.

[0015] In some embodiments, the zeolite incubator is further provided with a first inlet, and the liquid storage tank is connected to the first inlet through a fluid compression device, which can transport the culture medium in the liquid storage tank into the zeolite incubator.

[0016] The present invention achieves the following technical effects compared to the prior art:

[0017] This invention provides a large-scale Daphnia cultivation device and system. In use, the first outlet is initially closed, and the first light source is turned on. Due to phototaxis, the mother and juvenile Daphnia move towards the first light source. Then, a first sieve plate is installed between the first and second light sources. The first light source is then closed, and the second light source is turned on. The mother and juvenile Daphnia move towards the second light source, with the juveniles able to pass through the first sieve plate and reach the side of the first sieve plate closest to the second light source. The mother Daphnia is isolated on the side of the first sieve plate away from the second light source, thus achieving the sieving of large-scale Daphnia. Finally, a partition is installed on the side of the first sieve plate away from the second light source to separate the mother and juvenile Daphnia. The mother and juvenile daphnia are isolated on the side of the partition away from the first outlet. After the first outlet is opened, the original culture medium flows out and new culture medium is added, thus completing the function of culture medium replacement. Therefore, this large daphnia cultivation device and system can simultaneously realize the functions of large daphnia screening and culture medium replacement. Through the phototaxis of large daphnia and the first screening plate, the screening of all large daphnia in the daphnia incubator is completed in a short time without the need for individual separation, which improves screening efficiency. Through the phototaxis of large daphnia and the partition, the mother and juvenile daphnia are isolated on the side of the partition away from the first outlet, thus completing the culture medium replacement. In this process, there is no need to use a pipette to aspirate the daphnia, reducing the damage to the daphnia. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of a large-scale Daphnia cultivation device according to one embodiment of this invention;

[0020] Figure 2 This is a schematic diagram of the buoyancy switch structure according to one embodiment of this invention;

[0021] Figure 3 This is a schematic diagram of the structure of a large-scale Daphnia cultivation system according to one embodiment of this invention;

[0022] Figure 4 This is a schematic diagram of the feeding device structure according to one embodiment of this invention;

[0023] Figure 5 This is a schematic diagram of the structure of a filtration and circulation device according to one embodiment of this invention.

[0024] In the diagram: 1-Zephyranthes incubator; 11-First outlet; 12-First valve; 13-First light source; 14-Second light source; 15-Card slot; 16-Vibration device; 17-First inlet; 18-Second valve; 19-Buoyancy switch; 191-Float; 192-Lever frame; 193-Lever; 194-Positive contact; 195-Negative contact; 2-First sieve plate; 3-Baffle; 4-Second sieve plate; 5-Feeding device; 51-Algae incubator; 52-Cultivation lamp; 53-Circulation pump; 54-Third valve; 6-Filtration and circulation device; 61-Filter box; 62-Storage tank; 63-Fluid compression device; 7-Frame structure; 8-Controller. Detailed Implementation

[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] The purpose of this invention is to provide a large-scale Daphnia cultivation device and system to solve the technical problems existing in the prior art, and to simultaneously realize the functions of large-scale Daphnia screening and culture medium replacement, thereby improving screening efficiency and reducing cell damage.

[0027] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0028] Example 1

[0029] This embodiment provides a large-scale Daphnia cultivation device, such as... Figures 1-5As shown, the device includes a Daphnia incubator 1, a first sieving plate 2, and a partition 3. The Daphnia incubator 1 is used to hold culture medium, mother Daphnia, and young Daphnia. A first outlet 11 is provided on the bottom surface of the Daphnia incubator 1, and a first valve 12 is provided at the first outlet 11. The first valve 12 can control the opening or closing of the first outlet 11. A first light source 13 is provided near the first outlet 11, and a second light source 14 is provided away from the first outlet 11. The first sieving plate 2 is detachably installed inside the Daphnia incubator 1 and is positioned between the first light source 13 and the second light source 14. The first sieving plate 2 has a first sieving hole, the diameter of which is only large enough for juvenile daphnia to pass through. A partition 3 is detachably installed inside the daphnia incubator 1. The partition 3 is positioned between the first light source 13 and the first sieving plate 2, with the first outlet 11 located on the side of the partition 3 closest to the first light source 13. The partition 3 isolates the mother daphnia and juvenile daphnia on the side of the partition 3 away from the first outlet 11. In use, the first outlet 11 is first closed, and the first light source 13 is turned on. Due to phototaxis, the mother daphnia and juvenile daphnia move towards the first light source 13. Then, the daphnia and juvenile daphnia... A first screening plate 2 is installed between the second light sources 14. The first light source 13 is turned off and the second light source 14 is turned on. The female and juvenile daphnia move towards the second light source 14. The juvenile daphnia can pass through the first screening plate 2 and reach the side of the first screening plate 2 closest to the second light source 14, while the female daphnia is isolated on the side of the first screening plate 2 away from the second light source 14, thus achieving the screening of large daphnia. Then, a partition 3 is installed on the side of the first screening plate 2 away from the second light source 14, isolating the female and juvenile daphnia on the side of the partition 3 away from the first outlet 11. Finally, the first outlet 11 is opened, allowing... After the original culture medium flows out, new culture medium is added, thus completing the function of culture medium replacement. Therefore, this large-scale Daphnia cultivation device can simultaneously realize the functions of large-scale Daphnia screening and culture medium replacement. Through the phototaxis of large-scale Daphnia and the first screening plate 2, the screening of all large-scale Daphnia in the Daphnia incubator 1 is completed in a short time without the need for individual separation, which improves screening efficiency. Through the phototaxis of large-scale Daphnia and the partition plate 3, the mother Daphnia and the young Daphnia are isolated on the side of the partition plate 3 away from the first outlet 11, thus completing the culture medium replacement. In this process, there is no need to use a pipette to suck up the Daphnia, reducing damage to the Daphnia.

[0030] Specifically, large-scale Daphnia screening and culture medium replacement often overlap in time. When it is observed that the density of Daphnia in the culture chamber 1 is too high due to the large-scale reproduction of juvenile Daphnia, there will also be more food residue and molted skin in the culture medium. Therefore, replacing the culture medium at the same time as screening can save the time that staff spend on large-scale Daphnia screening and culture medium replacement, and improve work efficiency.

[0031] In some implementations of this embodiment, such as Figure 1As shown, the large-scale Daphnia cultivation device also includes a second sieving plate 4, which is detachably installed inside the Daphnia incubator 1. The second sieving plate 4 is positioned between the first sieving plate 2 and the partition plate 3. The second sieving plate 4 has second sieving holes, the diameter of which allows both mother and juvenile Daphnia to pass through. When changing the large-scale Daphnia sieving culture medium, the first light source 13 is turned on. Due to phototaxis, the mother and juvenile Daphnia move towards the first light source 13. Then, the first sieving plate 2 and the second sieving plate 4 are installed between the first light source 13 and the second light source 14. The first light source 13 is turned off and the second light source 14 is turned on. The mother daphnia and the juvenile daphnia move towards the second light source 14. Both the mother daphnia and the juvenile daphnia can pass through the second screening plate 4. The juvenile daphnia can pass through the first screening plate 2 and reach the side of the first screening plate 2 that is closer to the second light source 14. The mother daphnia is isolated between the first screening plate 2 and the second screening plate 4. Then, a partition 3 is installed on the side of the second screening plate 4 away from the second light source 14. By setting the second screening plate 4, the impact on the mother daphnia when installing the partition 3 can be reduced, and the damage to the daphnia body can be further reduced.

[0032] In some embodiments of this example, the aperture of the first screening plate 2 is 450-500μm, allowing only juvenile daphnia to pass through, while the aperture of the second screening plate 4 is 7-9mm, allowing both mother and juvenile daphnia to pass through. Both the first screening plate 2 and the second screening plate 4 are opaque black materials. Thus, when the first light source 13 is turned off and the second light source 14 is turned on, light can only pass through the screening holes on the first screening plate 2 or the second screening plate 4, providing better guidance for larger daphnia.

[0033] In some implementations of this embodiment, such as Figure 1 As shown, a slot 15 is provided on the inner wall of the Daphnia incubator 1. The first sieving plate 2, the second sieving plate 4 and the partition plate 3 can all be inserted into the corresponding slot 15 to realize the connection between the first sieving plate 2, the second sieving plate 4 and the partition plate 3 and the inner wall of the Daphnia incubator 1, thereby realizing the functions of large-scale Daphnia sieving and culture medium replacement.

[0034] In some implementations of this embodiment, such as Figure 1 As shown, the bottom surface of the Daphnia incubator 1 is inclined downwards towards the first outlet 11, so that the first outlet 11 is located at the bottom of the bottom surface of the Daphnia incubator 1. Food residue and molted skin produced by large Daphnia will be deposited towards the first outlet 11 under the action of gravity. When changing the culture medium, the first outlet 11 is opened, so that more food residue and molted skin in the original culture medium are discharged from the first outlet 11 under the action of the culture medium, which further improves the water quality in the Daphnia incubator 1.

[0035] In some implementations of this embodiment, such as Figure 1As shown, a vibration device 16 is installed on the outer side of the bottom surface of the Daphnia incubator 1. The vibration device 16 can vibrate the bottom surface of the Daphnia incubator 1. On the one hand, when food residue and molted skin adhere to the bottom wall of the Daphnia incubator 1, the vibration can separate the food residue and molted skin from the bottom surface of the Daphnia incubator 1. On the other hand, when food residue and molted skin block the first outlet 11, the vibration can clear the blockage.

[0036] In some embodiments of this example, the vibration device 16 can emit intermittent high-frequency vibrations, which disrupt the stable state of food residue and molted skin through pulsed energy, further preventing food residue and molted skin from adhering to the bottom surface of the Daphnia incubator 1 and preventing food residue and molted skin from clogging the first outlet 11.

[0037] In some implementations of this embodiment, such as Figure 1 As shown, the zeolite incubator 1 is also provided with a first inlet 17, and a second valve 18 is provided on the first inlet 17. The second valve 18 can control the opening and closing of the first inlet 17, thereby controlling the time when new culture medium is delivered into the zeolite incubator 1.

[0038] In some implementations of this embodiment, such as Figures 1-2 As shown, the large-scale Daphnia fusiforme cultivation device also includes a buoyancy switch 19. The buoyancy switch 19 is fixed to the inner wall of the Daphnia fusiforme incubator and located at the upper end of the first outlet 11. After the buoyancy switch 19 is triggered, the culture medium can enter the Daphnia fusiforme incubator 1 through the first inlet 17, thereby realizing the addition of new culture medium into the Daphnia fusiforme incubator 1.

[0039] In some implementations of this embodiment, such as Figure 2 As shown, the buoyancy switch 19 includes a float 191, a lever frame 192, a lever 193, a positive contact 194, and a negative contact 195. The lever frame 192 is fixed inside the daphnia incubator 1 near the first outlet 11. The lever 193 is hinged to the lever frame 192. One end of the lever 193 is provided with a float 191, which floats above the surface of the culture medium. The other end of the lever 193 is provided with a positive contact 194, and the negative contact 195 is located on the lever frame 192. When the culture medium decreases, the float 191 moves down, causing the lever 193 to rotate around the hinge point until the positive and negative contacts 194 and 195 contact, thereby closing the circuit and triggering the buoyancy switch 19. The circuit is then connected, allowing the culture medium to enter the daphnia incubator 1 through the first inlet 17, thus realizing the addition of new culture medium.

[0040] Example 2

[0041] This embodiment provides a large-scale Daphnia cultivation system, such as... Figures 3-5As shown, the device includes a feeding device 5, a filtration and circulation device 6, and the aforementioned large Daphnia cultivation device. The feeding device 5 is connected to the Daphnia incubator 1, and the filtration and circulation device 6 is connected to the first outlet 11. The feeding device 5 can provide algae food for the large Daphnia, and the filtration and circulation device 6 can filter out food residues and molted skins from the culture medium discharged from the Daphnia incubator 1 through the first outlet 11, thus creating a new culture medium. The new culture medium can be re-transported to the Daphnia incubator 1 through pipelines or added manually to the Daphnia incubator 1.

[0042] In some implementations of this embodiment, such as Figure 4 As shown, the feeding device 5 includes an algae culture tank 51, an algae culture lamp 52 is installed on the algae culture tank 51, and a circulation pump 53 is installed inside the algae culture tank 51. The algae are irradiated by the algae culture lamp 52 to promote algae growth. The circulation pump 53 installed inside the algae culture tank 51 can increase the contact area between the culture medium and the air by circulating the algae culture medium, improve the carbon dioxide dissolution efficiency, and at the same time, promptly discharge the oxygen produced by algae metabolism and make the algae solution evenly mixed, avoiding algae precipitation and clumping, which would cause uneven algae concentration in the algae solution flowing into the algae culture tank 1.

[0043] In some implementations of this embodiment, such as Figure 5 As shown, the filtration and circulation device 6 includes a filter box 61 and a storage tank 62. The first outlet 11 is connected to one end of the filter box 61, and the other end of the filter box 61 is connected to the storage tank 62. The filter box 61 can filter food residue and impurities such as molted skin, and store the filtered culture medium in the storage tank 62 to realize the recycling of the culture medium. The culture medium in the storage tank 62 can be transported back to the zeolite incubator 1 through pipelines, or it can be added to the zeolite incubator 1 manually.

[0044] In some implementations of this embodiment, such as Figure 1 and 5 As shown, the zeolite incubator 1 is also provided with a first inlet 17. The storage tank 62 is connected to the first inlet 17 through a fluid compression device 63. The fluid compression device 63 can transport the culture medium in the storage tank 62 to the zeolite incubator 1 to complete the replacement of the culture medium and realize the recycling of the culture medium.

[0045] In some embodiments of this example, the second valve 18 and the fluid compression device 63 are both signal-connected to the buoyancy switch 19. When the buoyancy switch 19 is triggered, it can control the second valve 18 and the fluid compression device 63 to open, so as to transport the culture medium in the storage tank 62 to the zeolite incubator 1 through the first inlet 17.

[0046] In some implementations of this embodiment, such as Figure 3As shown, the large-scale Daphnia cultivation system also includes a three-layer frame structure 7, in which the large-scale Daphnia cultivation device is located in the middle layer of the frame structure 7, the feeding device 5 is located in the top layer of the frame structure 7, and the filtration and circulation device 6 is located in the bottom layer of the frame structure 7. When the first valve 12 is opened, the culture medium in the Daphnia culture tank 1 is transported to the filter box 61 through the first outlet 11 under the action of gravity. The bottom end of the feeding device 5 is connected to the Daphnia culture tank 1 through a pipeline, and a third valve 54 is installed on the connecting pipeline. When the third valve 54 is opened, the algae in the algae culture tank 51 can be transported to the Daphnia culture tank 1 along the pipeline under the action of gravity. By setting up a three-layer frame structure 7, the filtration and collection of the culture medium and the feeding of large-scale Daphnia can be realized by using gravity, saving energy. Moreover, the feeding device 5, the filtration and circulation device 6 and the large-scale Daphnia cultivation device are arranged vertically in layers, saving the floor space of the large-scale Daphnia cultivation system.

[0047] In some implementations of this embodiment, such as Figure 3 As shown, the large-scale Daphnia cultivation system also includes a controller 8. The first valve 12, the second valve 18, and the third valve 54 are all solenoid valves. The controller 8 is signal-connected to the first valve 12, the second valve 18, the third valve 54, and the fluid compression device 63. The controller 8 can control the opening or closing of the third valve 54, thereby controlling the feeding device 5 to feed algae into the Daphnia cultivation tank 1 at regular intervals. The controller 8 can control the opening or closing of the first valve 12, thereby causing the culture medium in the Daphnia cultivation tank 1 to be discharged through the first outlet 11.

[0048] In some embodiments of this example, the Daphnia macrocarpa cultivation system is set up in a dark indoor environment, which can better utilize the phototaxis of Daphnia macrocarpa to guide its behavior, thereby improving the screening efficiency and the efficiency of culture medium replacement.

[0049] Specific examples have been used to illustrate the principles and implementation methods of this invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this invention. Furthermore, those skilled in the art will recognize that, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. A large-scale Daphnia cultivation device, characterized in that, include A daphne incubator is provided, which is used to place culture medium, mother daphne and young daphne. The bottom surface of the daphne incubator is provided with a first outlet, and a first valve is provided at the first outlet. The first valve can control the opening or closing of the first outlet. A first light source is provided near the first outlet and a second light source is provided away from the first outlet. The first sieve plate is detachably installed inside the Daphnia incubator. The first sieve plate is positioned between the first light source and the second light source. The first sieve plate has a first sieve hole, the diameter of which is only large enough for young Daphnia to pass through. as well as A partition is detachably installed inside the daphnia incubator. The partition is located between the first light source and the first screening plate, and the first outlet is located on the side of the partition closer to the first light source. The partition can isolate the mother daphnia and the juvenile daphnia on the side of the partition away from the first outlet.

2. The large-scale Daphnia cultivation device according to claim 1, characterized in that, It also includes a second sieving plate, which is detachably installed inside the Daphnia incubator. The second sieving plate is disposed between the first sieving plate and the partition plate. The second sieving plate has a second sieving hole, the diameter of which allows the mother Daphnia and the juvenile Daphnia to pass through.

3. The large-scale Daphnia cultivation device according to claim 1, characterized in that, The bottom surface of the daphne incubator is inclined downwards towards the first outlet.

4. The large-scale Daphnia cultivation device according to claim 3, characterized in that, A vibration device is installed on the outer side of the bottom surface of the daphne incubator, which can cause the bottom surface of the daphne incubator to vibrate.

5. The large-scale Daphnia cultivation device according to claim 3, characterized in that, The zeolite incubator is also provided with a first inlet, and a second valve is provided on the first inlet, which can control the opening and closing of the first inlet.

6. The large-scale Daphnia cultivation device according to claim 5, characterized in that, It also includes a buoyancy switch, which is fixed to the inner wall of the daphne incubator and located above the first outlet. When the buoyancy switch is triggered, the culture medium enters the daphne incubator through the first inlet.

7. A large-scale Daphnia cultivation system, characterized in that, The device includes a feeding device, a filtration and circulation device, and a large-scale Daphnia cultivation device as described in any one of claims 1 to 6, wherein the feeding device is connected to the Daphnia incubator, and the filtration and circulation device is connected to the first outlet.

8. The large-scale Daphnia cultivation system according to claim 7, characterized in that, The feeding device includes an algae culture box, which is equipped with a culture lamp and a circulation pump.

9. The large-scale Daphnia cultivation system according to claim 7, characterized in that, The filtration and circulation device includes a filter box and a liquid storage tank. The first outlet is connected to one end of the filter box, and the other end of the filter box is connected to the liquid storage tank. The filter box is capable of filtering impurities.

10. The large-scale Daphnia cultivation system according to claim 9, characterized in that, The zeolite incubator is also provided with a first inlet, and the liquid storage tank is connected to the first inlet through a fluid compression device, which can transport the culture medium in the liquid storage tank into the zeolite incubator.

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