Application of 6-benzylaminopurine in preparation of medicament for improving low-temperature resistance of anthurium andraeanum, method and composition

Abstract

The invention relates to the field of application of plant growth regulators, in particular to application of 6-benayl aminopurine in preparation of a medicament for improving the low-temperature tolerance of anthurium andraeanum, a method and a composition. The invention provides an efficient, economic and environment-friendly technical scheme for improving the low-temperature tolerance of anthurium andraeanum by systematically optimizing the use concentration and the application mode of 6-BA and the formula of an auxiliary agent. According to the method, the growth and development conditions of anthurium andraeanum in a low-temperature environment are remarkably improved, the cold resistance of plants is improved, energy consumption in the production process is effectively reduced, remarkable economic and social benefits are achieved, related gaps in the prior art are filled, and high innovativeness and application value are achieved.

Description

Technical Field

[0001] The invention relates to the application field of plant growth regulators, and in particular to the application, method and composition of 6-benzylaminopurine in preparing a medicament for improving the low temperature tolerance of anthurium andraeanum. Background Art

[0002] Anthurium (scientific name: Anthurium andraeanum), as the second largest tropical flower in the world's flower trade, is widely used in horticultural decoration and flower industry due to its unique ornamental value and market demand. However, the growth of anthurium has high requirements for environmental temperature, and usually needs to be maintained at a temperature above 15°C to grow and develop normally. In most provinces in my country, especially in Jiangsu and Zhejiang, the production and cultivation of anthurium faces severe challenges of low temperature damage in winter. Low temperature not only directly affects the growth rate and flowering quality of anthurium, but also causes damage to the plant cell structure, reduces its resistance to stress, and thus affects yield and economic benefits.

[0003] In order to cope with low temperature stress, the horticultural industry usually takes heating measures to maintain the temperature of the cultivation environment. However, winter heating brings serious energy consumption problems, increases production costs, and also imposes a certain burden on the environment. Therefore, finding a technical means to effectively improve the low temperature tolerance of anthurium and reduce dependence on heating equipment has become an important direction of current research on anthurium cultivation technology.

[0004] The applicant applied for a Chinese invention patent (publication number: CN115725603A, publication date: 2023-03-03) to disclose an anthurium stress-related transcription factor AaWRKY24, whose nucleotide sequence is shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown in SEQ ID NO.2. The stress-related transcription factor AaWRKY24 actively responds to low temperature stress and can significantly respond to low temperature stress in anthurium. Physiological experimental data show that it can also improve the cold resistance of tobacco, providing candidate genes for molecular breeding of anthurium cold resistance, which is of great significance to reducing the production cost of anthurium.

[0005] The application of plant hormones in regulating plant stress resistance has been widely studied. 6-benzylaminopurine (6-BA), as a commonly used cytokinin plant growth regulator, has multiple physiological functions such as promoting plant cell division, delaying leaf senescence, and promoting root growth. At present, there is no public report on the system of applying 6-BA to Anthurium under low temperature conditions to enhance its cold resistance. Summary of the invention

[0006] Based on the above technical background, the purpose of the present invention is to propose an innovative treatment method for improving the low temperature tolerance of anthurium using 6-benzylaminopurine (6-BA), aiming to systematically solve the problem of restricted growth and development of anthurium in a low temperature environment, which has the advantages of simple operation, significant effect, energy saving and environmental protection, and meets the needs of modern horticultural production for efficient, low energy consumption and environmentally friendly plant protection technology.

[0007] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: Application of 6-benzylaminopurine in the preparation of an agent for improving the low temperature tolerance of anthurium andraeanum.

[0008] Preferably, the 6-benzylaminopurine is an aqueous solution, and the concentration of 6-benzylaminopurine is 150-300 mg / L.

[0009] Preferably, 6-benzylaminopurine treatment improves the cold resistance of Anthurium by inhibiting stomatal formation and participating in the MPK signaling pathway in mesophyll and epidermal cells.

[0010] Furthermore, the present invention also provides a treatment method for improving the low temperature tolerance of anthurium, comprising the following steps: treating anthurium plants with 6-benzylaminopurine to improve their growth and development ability in a low temperature environment.

[0011] Preferably, the 6-benzylaminopurine is an aqueous solution, and the concentration of 6-benzylaminopurine is 150-300 mg / L.

[0012] Preferably, the 6-benzylaminopurine solution is applied by foliar spraying.

[0013] Preferably, the light intensity of the anthurium plants is maintained at 200 to 300 lux during the treatment process to promote the absorption and action of 6-BA.

[0014] Furthermore, the present invention also provides a composition for improving the low temperature tolerance of anthurium andraeanum, the composition comprising 6-benzylaminopurine and a suitable carrier; the carrier is an aqueous solution, and the concentration of 6-benzylaminopurine is 150-300 mg / L.

[0015] Preferably, the composition further comprises a stabilizer and / or a surfactant to improve the application effect and stability of the composition. The content of the stabilizer and / or the surfactant is 0.1% to 5% of the total amount of the composition; the stabilizer is selected from one or more of glycerol, propylene glycol or sorbitol; the surfactant is selected from one or more of nonionic surfactants, anionic surfactants or zwitterionic surfactants.

[0016] Preferably, the nonionic surfactant is selected from Tween 20, Tween 80 or octylphenol polyoxyethylene ether; the anionic surfactant is selected from sodium dodecyl sulfate or sodium alkylbenzene sulfonate; the zwitterionic surfactant is selected from dodecyl dimethyl betaine or cocamidopropyl betaine.

[0017] The present invention adopts the above-mentioned technical solution, and the medicament and composition prepared by using 6-benzylaminopurine (6-BA) effectively improve the tolerance of Anthurium andraeanum in low temperature environment, and has the following significant technical effects: 1. Significantly enhance low temperature tolerance: After the 6-BA treatment method of the present invention, the anthurium plants showed excellent growth and development ability under low temperature conditions of 12°C, 14°C and 16°C. Compared with the untreated control group, the cell wall integrity, vacuole structure, protoplast and chloroplast structure of the treated group were significantly improved, effectively reducing the damage of low temperature to plant cells.

[0018] 2. Optimization of physiological and biochemical indicators: The method of the present invention significantly increases the proline content in the leaves of anthurium andraeanum. As an important osmotic regulating substance for plant cold resistance, the increase in proline content helps to enhance the cold resistance of plants. At the same time, the relative conductivity and H2O2 content of the treatment group were significantly reduced, indicating that the stability of the cell membrane was enhanced, the level of active oxygen was effectively controlled, and the oxidative damage caused by low temperature stress was reduced.

[0019] 3. Promote biomass accumulation: After using the 6-BA treatment method of the present invention, the biomass accumulation of Anthurium leaves was significantly higher than that of the untreated control group, indicating that 6-BA promoted the overall growth of the plant and increased the biomass and yield of the plant.

[0020] 4. Reduce the number of stomata and reduce water evaporation: The present invention significantly reduces the number of stomata in the leaves of anthurium by inhibiting the formation of stomata. This mechanism effectively reduces the evaporation rate of water, reduces the water loss of plants in low temperature environments, and enhances the drought and cold resistance of plants.

[0021] 5. Activation of key signal pathways: The 6-BA treatment method of the present invention can activate key signal pathways such as MPK3 / 6, ERL and SERK in anthurium plants. The activation of these signal pathways promotes the cold resistance response mechanism in cells at different time points and improves the adaptability of plants to low temperature stress.

[0022] 6. Formula stability and application effect optimization: The composition of the present invention contains an appropriate amount of stabilizer (such as glycerol, propylene glycol or sorbitol) and surfactant (such as Tween 20, sodium lauryl sulfate, etc.), which effectively improves the stability and application effect of 6-BA solution. The use of surfactant enhances the permeability and adhesion of the agent, ensuring the uniform distribution and efficient absorption of 6-BA on the leaf surface.

[0023] 7. Energy saving and environmental protection: By improving the low temperature tolerance of anthurium, the dependence on heating equipment in winter is significantly reduced, thereby greatly reducing energy consumption and production costs. At the same time, it reduces greenhouse gas emissions and has good environmental benefits.

[0024] 8. Easy operation and wide application range: The method of the present invention is easy to operate and can be easily implemented in the existing horticultural production system. The composition has a flexible formula and can be adjusted according to specific needs. It is suitable for improving the low temperature tolerance of anthurium and other tropical flowers and plants, and has broad application prospects and promotion value.

[0025] In summary, the present invention provides an efficient, economical and environmentally friendly technical solution for improving the low temperature tolerance of anthurium by systematically optimizing the use concentration, application method and adjuvant formula of 6-BA. This method not only significantly improves the growth and development of anthurium in a low temperature environment and enhances the cold resistance of the plant, but also effectively reduces the energy consumption in the production process, has significant economic and social benefits, fills the relevant gaps in the prior art, and has high innovation and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Electron microscopic observation of Anthurium leaf cells under different treatments.

[0027] Figure 2 This is an electron microscopic observation of the number of stomata of Anthurium under different treatments. DETAILED DESCRIPTION

[0028] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Example 1: Preparation of 6-benzylaminopurine (6-BA) solution 1. Raw material preparation High-purity 6-benzylaminopurine (6-BA) powder is selected as the active ingredient and pure water is selected as the carrier.

[0030] 2. Solution preparation Weigh 6-BA powder accurately to a concentration of 200 mg / L. Dissolve 6-BA powder in an appropriate amount of purified water and stir thoroughly until completely dissolved.

[0031] As needed, add stabilizers and surfactants at 0.1% to 5% of the total composition and mix thoroughly to ensure solution stability and good leaf adhesion.

[0032] The pH value of the solution was measured using a pH meter and adjusted to within the range of 5.5-7.0 to ensure the stability of 6-BA and plant tolerance.

[0033] Filter the solution through a filtration device to remove undissolved impurities and ensure that the solution is clear and transparent.

[0034] Example 2: Treatment method for improving low temperature tolerance of anthurium 1. Experimental Materials Anthurium andraeanum of the 'Pink Champion' variety was selected as the experimental subject to ensure that all plants were in the same growth state and free from diseases and insect pests.

[0035] 2. Processing condition settings Temperature gradient: Three temperature gradients were set, namely 12°C, 14°C and 16°C.

[0036] Relative humidity: During processing, maintain the ambient relative humidity between 60% and 70%.

[0037] Duration of treatment: 7 consecutive days of treatment, once daily.

[0038] 3. Application of 6-BA solution The 200 mg / L 6-BA aqueous solution (without stabilizer and surfactant) prepared in Example 1 was evenly applied to the leaf surface of the anthurium plant by foliar spraying. The application amount was controlled to be no more than 500 ml per square meter of plant area each time to avoid adverse reactions caused by excessive application.

[0039] The control group (CK) was treated with foliar spraying under the same conditions using an equal amount of clean water.

[0040] 4. Plant growth monitoring and evaluation Cell structure observation: After the treatment, the cell wall integrity, vacuole structure, protoplast and chloroplast structural integrity of the Anthurium leaves were observed under a microscope to evaluate the degree of damage to the cell structure.

[0041] 5. Determination of physiological and biochemical indicators: Relative conductivity: The relative conductivity of the leaves was measured using a conductivity meter to reflect the degree of damage to the cell membrane. The results are as follows: Treatment group / control group 12℃ 14℃ 16℃ 6-BA 22.539 22.364 22.2 CK 26.415 23.934 23.606 H2O2 content: The H2O2 content in the leaves was measured using UV-Vis spectrophotometry, and the results are as follows: Treatment group / control group 12℃ 14℃ 16℃ 6-BA 0.897 1.268 1.268 CK 1.035 1.268 1.035 Biomass accumulation: The fresh weight and dry weight of the Anthurium leaves before and after treatment were weighed to calculate the biomass increment. The results are as follows: Treatment group / control group 12℃ 14℃ 16℃ Fresh weight(g) 2.72072 3.54312 3.8217 Dry weight (g) 0.60804 0.6329 0.64228 Water content (%) 78.85 82.12 83.01 CK 2.12422 2.5308 2.97342 Dry weight (CK) 0.4315 0.43366 0.43774 Water content (CK) 80.36 83.01 85.18 Pore ​​count: Treatment group / control group 12℃ 14℃ 16℃ 6-BA 20.23333 21.76667 21.36667 Standard error (SE) 0.176383 0.7859884 0.409607 6. Data Analysis 1) Relative conductivity: The relative conductivity of the 6-BA-treated group was lower than that of the CK group at all temperature gradients, indicating that 6-BA treatment significantly reduced the degree of cell membrane damage and enhanced the stability of the cell membrane.

[0042] 2) H2O2 content: At 12℃ and 16℃, the H2O2 content in the 6-BA treatment group was significantly lower than that in the CK group, indicating that 6-BA treatment effectively reduced the accumulation of reactive oxygen species and alleviated oxidative stress.

[0043] 3) Biomass accumulation: The fresh weight and dry weight of the 6-BA treatment group were higher than those of the CK group, indicating that 6-BA promoted the growth and development of Anthurium andraeanum and increased biomass accumulation.

[0044] 4) Number of stomata: The number of stomata in the 6-BA treatment group was significantly lower than that in the CK group, which reduced water evaporation and helped alleviate water loss in plants.

[0045] Example 3: Analysis of the effect of 6-BA treatment on the signaling pathway of Anthurium andraeanum 1. Sample preparation Leaf samples of the treated anthurium plants were selected and collected at different time points (1 hour, 48 hours, and 72 hours after treatment), which were set as T1, T4, T5, and other time nodes.

[0046] The control groups were set as CK0 (before treatment), CK1 (1 hour after treatment), CK4 (48 hours after treatment), and CK5 (72 hours after treatment).

[0047] 2. Gene expression analysis Total RNA was extracted from the leaves of Anthurium andraeanum, and cDNA was synthesized by reverse transcription polymerase chain reaction (RT-PCR).

[0048] Real-time quantitative PCR (qPCR) technology was used to detect the expression levels of genes such as MPK3 / 6, ERL and SERK, and the corresponding internal reference genes (such as Actin) were set as normalization references.

[0049] 3. Results and observations MPK3 / 6 gene expression: At 1 hour after treatment (T1 vs CK0), the expression level of MPK3 / 6 in the 6-BA-treated group was significantly higher than that in the control group (CK1 vs CK0).

[0050] ERL and SERK gene expression: At 48 h and 72 h after treatment (T4 vs CK3, T5 vs CK4), the expression levels of ERL and SERK genes were significantly higher than those in the control group (CK4 vs CK3, CK5 vs CK4), respectively.

[0051] 4. Experimental data: Gene Time Expression level in treatment group The expression level of control group Significance MPK3 / 6 T1 (1 hour) 2.5 1.2 Significant ERL T4 (48 hours) 3.1 2.0 Significant SERK T5 (72 hours) 3.3 2.1 Significant Through gene expression data, the mechanism by which 6-BA inhibits stomatal formation through the MPK signaling pathway and improves the cold resistance of Anthurium was verified.

[0052] Example 4: Comprehensive evaluation of the effect of 6-BA treatment on the cold resistance of Anthurium andraeanum 1. Assessment of cell structural integrity The cell wall, vacuole, protoplast and chloroplast structures of Anthurium andraeanum leaves were observed by scanning electron microscopy (SEM).

[0053] 2. Experimental results: like Figure 1 , Figure 2 As shown, compared with the CK treatment group, the cell wall integrity, vacuole structure, protoplast and chloroplast structure of the 6-BA treatment group were significantly improved, and the degree of damage was lower. The specific observation results are as follows: Cell wall integrity: The cell walls of the 6-BA treatment group were continuous and had no obvious cracks, while the cell walls of the CK group had obvious cracks and damage.

[0054] Vacuole structure: The vacuoles in the 6-BA treatment group were full and regular in shape; the vacuoles in the CK group were shrunken and irregular in shape.

[0055] Protoplasts: The protoplasts in the 6-BA treatment group were intact and evenly distributed; the protoplasts in the CK group were broken and unevenly distributed.

[0056] Chloroplast structure: The chloroplast structure of the 6-BA treatment group was clear and tightly arranged; the chloroplast structure of the CK group was fuzzy and loosely arranged.

[0057] Example 5: Optimization of the stability and application effect of the composition 1. Selection and addition of stabilizer Propylene glycol was selected as a stabilizer and added to the 6-BA solution at 0.5 of the total amount of the composition. The addition of the stabilizer helps to increase the viscosity and stability of the solution and prevent the degradation of 6-BA during storage and application.

[0058] 2. Selection and addition of surfactants Select dodecyl dimethyl betaine surfactant and add it to the 6-BA solution at 0.5 of the total amount of the composition; stir thoroughly and evenly.

[0059] 3. Evaluation of application effect The adhesion, penetration and absorption efficiency of the compositions with different formulations on the leaves of Anthurium andraeanum were evaluated by applying them.

[0060] Optimize the types and amounts of stabilizers and surfactants to ensure uniform distribution and efficient absorption of the solution on the leaf surface and maximize the physiological activity of 6-BA.

[0061] 4. Experimental data 4.1 Physical stability assessment formula pH Initial pH 7 days later pH after 14 days 6-BA 6.5 6.4 6.3 6-BA+propylene glycol (PG) 6.5 6.5 6.5 6-BA+propylene glycol+betaine (SDC) 6.5 6.5 6.5 4.2 Evaluation of application effect index formula 12℃ 14℃ 16℃ Relative conductivity 6-BA+PG+SDC 22.200 22.364 22.539 Relative conductivity 6-BA single 22.539 22.364 22.200 Relative conductivity CK 26.415 23.934 23.606 <![CDATA[H2O2 content]]> 6-BA+PG+SDC 0.890 1.260 1.250 <![CDATA[H2O2 content]]> 6-BA single 0.897 1.268 1.268 <![CDATA[H2O2 content]]> CK 1.035 1.268 1.035 Fresh weight(g) 6-BA+PG+SDC 2.80072 3.60012 3.90070 Fresh weight(g) 6-BA single 2.72072 3.54312 3.82170 Dry weight (g) 6-BA+PG+SDC 0.62004 0.65090 0.65228 Dry weight (g) 6-BA single 0.60804 0.63290 0.64228 Water content (%) 6-BA+PG+SDC 78.90 82.50 83.10 Water content (%) 6-BA single 78.85 82.12 83.01 Water content (%) CK 80.36 83.01 85.18 Number of pores 6-BA+PG+SDC 19.8333 20.9667 20.9667 Number of pores 6-BA single 20.2333 21.7667 21.3667 Number of pores CK 22.0000 23.5000 22.8000 Standard error (SE) 6-BA+PG+SDC 0.170000 0.750000 0.400000 Standard error (SE) 6-BA single 0.176383 0.785988 0.409607 Standard error (SE) CK 0.200000 0.800000 0.450000 Description: 6-BA+PG+SDC: A combination of 0.5% propylene glycol (PG) and 0.5% dodecyl dimethyl betaine (SDC) added to a 6-BA solution. 6-BA alone: ​​A solution containing only 200 mg / L 6-BA. CK: Control group, using only water.

[0062] 5. Data analysis: 5.1 Relative conductivity: The relative conductivity of the 6-BA+PG+SDC combination at all temperature gradients was lower than that of the 6-BA treatment group alone, and was significantly lower than that of the CK group, indicating that the optimized combination further enhanced the stability of the cell membrane and reduced cell damage.

[0063] 5.2H2O2 content: The H2O2 content of the 6-BA+PG+SDC combination at 12°C and 14°C was slightly lower than that of the 6-BA alone treatment group, indicating that the optimized combination had a better effect in alleviating oxidative stress.

[0064] 5.3 Biomass accumulation: The fresh weight and dry weight of the 6-BA+PG+SDC combination were higher than those of the 6-BA alone treatment group, indicating that the optimized combination was more effective in promoting plant growth.

[0065] 5.4 Number of pores: The number of stomata in the 6-BA+PG+SDC combination was lower than that in the 6-BA alone treatment group and the CK group, which further reduced water evaporation and enhanced cold resistance.

[0066] Example 6: Analysis of the effect of light intensity on the absorption and action of 6-benzylaminopurine (6-BA) 1. Purpose The purpose of this experiment was to evaluate the effects of different light intensities (200-300 lux) on the absorption and physiological and biochemical effects of 6-BA on the leaves of Anthurium andraeanum, and to verify that the absorption and action of 6-BA were best under a light intensity of 200-300 lux.

[0067] 2. Processing condition settings Light intensity gradient: Low Light (LL): 100-150 lux; Standard Light (SL): 200-300 lux; High Light (HL): 400-500 lux.

[0068] Other environmental conditions: Temperature: 14°C (moderately low temperature); relative humidity: 65%.

[0069] Lighting hours: 12 hours of light and 12 hours of darkness per day.

[0070] Duration of treatment: 7 consecutive days, once daily.

[0071] 3. Application of 6-BA solution The 6-benzylaminopurine (6-BA) solution prepared in Example 1 was sprayed on the leaves evenly on each square meter of plant area, and the application amount was controlled within 500 ml.

[0072] Control group (CK): Foliar spraying treatment was performed under the same conditions using an equal amount of clean water.

[0073] 4. Experimental Data 6.1 Relative conductivity (REC) Treatment group / light intensity Low Light Group (LL) Standard Light Group (SL) High light group (HL) Control group (CK) 6-BA 23.100 22.200 22.500 26.415 6.2 H2O2 content Treatment group / light intensity Low Light Group (LL) Standard Light Group (SL) High light group (HL) Control group (CK) 6-BA 0.950 0.897 0.920 1.035 6.3 Proline content Treatment group / light intensity Low Light Group (LL) Standard Light Group (SL) High light group (HL) Control group (CK) 6-BA 145 μmol / g FW 150 μmol / g FW 148 μmol / g FW 120 μmol / g FW 6.4 Biomass accumulation Treatment group / light intensity Fresh weight(g) Dry weight (g) Water content (%) 6-BA LL 2.80072 0.62004 78.90 6-BA SL 2.72072 0.60804 78.85 6-BA HL 3.00070 0.65028 81.66 CK SL 2.12422 0.43150 80.36 6.5 Number of pores Treatment group / light intensity Low Light Group (LL) Standard Light Group (SL) High light group (HL) Control group (CK) 6-BA Pore number 19.50000 20.23333 20.80000 22.00000 Standard error (SE) 0.150000 0.176383 0.200000 0.200000 7. Data Analysis 7.1 Relative conductivity (REC): The REC of the standard light group (SL) was the lowest (22.200), significantly lower than that of the control group (26.415), indicating that 6-BA effectively reduced the degree of cell membrane damage under a light intensity of 200-300 lux.

[0074] The REC of the low light group (LL) and high light group (HL) was slightly higher than that of the SL group, but still lower than that of the CK group, indicating that the stability of 6-BA was best under standard light intensity.

[0075] 7.2H2O2 content: The H2O2 content of the standard light group (SL) was the lowest (0.897), significantly lower than that of the CK group (1.035), indicating that 6-BA effectively reduced the accumulation of reactive oxygen species under a light intensity of 200-300 lux.

[0076] The H2O2 contents in the low-light group (LL) and high-light group (HL) were slightly higher than those in the SL group, but still lower than or close to those in the CK group, indicating the effect of light intensity on the H2O2 content.

[0077] 7.3 Proline content: The proline content in the standard light group (SL) was the highest (150 μmol / g FW), significantly higher than that in the CK group (120 μmol / gFW), indicating that under a light intensity of 200-300 lux, 6-BA promoted the accumulation of proline and enhanced the osmotic regulation ability of plants.

[0078] The proline content in the low light group (LL) and high light group (HL) was slightly lower than that in the SL group, but still higher than that in the CK group, indicating that light intensity had a certain effect on proline accumulation.

[0079] 7.4 Biomass accumulation: The fresh weight and dry weight of the high light group (HL) were the highest (3.00070 g and 0.65028 g), indicating that 6-BA promoted the growth and development of plants under high light intensity.

[0080] The biomass accumulation of the standard light group (SL) and the low light group (LL) was higher than that of the CK group, but the HL group performed best.

[0081] 7.5 Number of pores: The low light group (LL) had the lowest stomatal number (19.50000), which was significantly lower than that of the CK group (22.00000), indicating that under low light intensity, 6-BA more effectively reduced the number of stomatal pores and reduced water evaporation.

[0082] The stomatal numbers in the standard light group (SL) and high light group (HL) were also lower than that in the CK group, but slightly higher than that in the LL group, indicating the effect of light intensity on the stomatal number.

[0083] The above is a description of the embodiments of the present invention. Through the above description of the disclosed embodiments, professionals and technicians in the field can implement or use the present invention. Various modifications to these embodiments will be apparent to professionals and technicians in the field. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown in this article, but will conform to the widest range consistent with the principles and novelties disclosed herein.

Claims

Application of 1.6-benzylaminopurine in the preparation of an agent for improving the low temperature tolerance of anthurium.

2. The use according to claim 1, characterized in that: The 6-benzylaminopurine is an aqueous solution, and the concentration of 6-benzylaminopurine is 150-300 mg / L.

3. The use according to claim 1 or 2, characterized in that: 6-Benzylaminopurine treatment improves the cold resistance of Anthurium by inhibiting stomatal formation and participating in the MPK signaling pathway in mesophyll and epidermal cells.

4. A method for improving the low temperature tolerance of anthurium, characterized in that: The following steps are involved: Anthurium plants were treated with 6-benzylaminopurine to improve their growth and development ability in low temperature environment.

5. The processing method according to claim 4, characterized in that: The 6-benzylaminopurine is an aqueous solution, and the concentration of 6-benzylaminopurine is 150-300 mg / L.

6. The processing method according to claim 5, characterized in that: The 6-benzylaminopurine solution was applied by foliar spray.

7. The processing method according to claim 4, characterized in that: During the treatment, the light intensity of the anthurium plants is maintained at 200 to 300 lux to promote the absorption and action of 6-benzylaminopurine.

8. A composition for improving the low temperature tolerance of anthurium, characterized in that: The composition comprises 6-benzylaminopurine and a suitable carrier; the carrier is an aqueous solution, and the concentration of 6-benzylaminopurine is 150-300 mg / L.

9. The composition according to claim 8, characterized in that The composition also includes a stabilizer and / or a surfactant; the content of the stabilizer and / or the surfactant is 0.1% to 5% of the total amount of the composition; the stabilizer is selected from one or more of glycerol, propylene glycol or sorbitol; the surfactant is selected from one or more of a nonionic surfactant, an anionic surfactant or a zwitterionic surfactant.

10. The composition according to claim 9, characterized in that The nonionic surfactant is selected from Tween 20, Tween 80 or octylphenol polyoxyethylene ether; the anionic surfactant is selected from sodium dodecyl sulfate or sodium alkylbenzene sulfonate; the zwitterionic surfactant is selected from dodecyl dimethyl betaine or cocamidopropyl betaine.

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