The invention provides a
tissue culture and rapid propagation method for potted
anthurium andraeanum seedlings, and relates to the technical field of
tissue culture of potted
anthurium andraeanum seedlings. The
tissue culture and rapid propagation method for the potted
anthurium andraeanum seedlings comprises the following tissue culture methods: step 1, explant selection and treatment: (1) taking stem segments as explants, and selecting tender and non-lignified parts; (2) washing the explant stem with
tap water for 10 minutes to remove surface impurities, sequentially soaking the explant stem with 75%
alcohol for 30-60 seconds, soaking the explant stem with 0.1%
mercury bichloride liquid for 8 minutes, rinsing the explant stem with
sterile water for 4-6 times, and soaking the explant stem into the
sterile water for later use; and (3) in an ultra-clean
workbench, using sterilized
filter paper to absorb
moisture,
cutting the stem segments into small segments of 0.5-1cm, keeping 1-2 stem nodes in each segment, and adding an anti-
browning agent when the
cut of the stem segments is easy to brown. The anti-
browning agent with the concentration of 50-100 mg / L and the anti-
browning agent
activated carbon with the concentration of 0.5%-2.5% are added, browning products are adsorbed, the permeability of the culture medium is adjusted, high-efficiency
oxidation resistance can be achieved through the operation method, the
activated carbon adsorbs phenolic substances,
ascorbic acid neutralizes free radicals, and browning is synergistically reduced.