Induction and detection method for androgenesis dihaploid of Paralichthys olivaceus

A technology for androgenesis, double haploid, applied in fish farming, application, climate change adaptation, etc. Simple to use effects

Active Publication Date: 2016-01-13
中国水产科学研究院北戴河中心实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, it is not very convenient to induce androgenesis in fish by irradiation inactivation

Method used

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  • Induction and detection method for androgenesis dihaploid of Paralichthys olivaceus
  • Induction and detection method for androgenesis dihaploid of Paralichthys olivaceus
  • Induction and detection method for androgenesis dihaploid of Paralichthys olivaceus

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Embodiment 1

[0049] A method for inducing and detecting double haploids in flounder androgen, comprising the following steps:

[0050] 1) Collection and screening of sperm and eggs: collect fresh male flounder sperm and female flounder eggs, after collecting the male sperm and female ova, store them in the dark, and check the eggs and eggs under a dissecting mirror and microscope. Sperm screening, during the screening process, select transparent, plump, uniform-sized eggs and sperm with a long swimming time;

[0051] 2) Artificial insemination: dilute the sperm with Ringer's solution, the volume ratio of the Ringer's solution to the sperm is 1:30 to obtain a sperm dilution, mix the sperm dilution with the egg, and the result is The volume ratio of the sperm diluent and the egg is 1:10, after stirring evenly, adding seawater to it for activation, the temperature of the seawater is 15°C, and the sperm-egg mixture activated by seawater is obtained;

[0052] 3) Cold shock treatment induces an...

Embodiment 2

[0071] A method for inducing and detecting double haploids in flounder androgen, comprising the following steps:

[0072] 1) Collection and screening of sperm and eggs: collect fresh male flounder sperm and female flounder eggs, after collecting the male sperm and female ova, store them in the dark, and check the eggs and eggs under a dissecting mirror and microscope. Sperm screening, during the screening process, select transparent, plump, uniform-sized eggs and sperm with a long swimming time;

[0073] 2) Artificial insemination: Dilute the sperm with Ringer's solution, the volume ratio of the Ringer's solution to the sperm is 1:50 to obtain a sperm dilution, mix the sperm dilution with the egg, and the result is The volume ratio of the sperm diluent to the egg is 1:30, after stirring evenly, adding seawater to it for activation, the temperature of the seawater is 17°C, and the sperm-egg mixture activated by seawater is obtained;

[0074] 3) Cold shock treatment induces and...

Embodiment 3

[0092] A method for inducing and detecting double haploids in flounder androgen, comprising the following steps:

[0093] 1) Collection and screening of sperm and eggs: collect fresh male flounder sperm and female flounder eggs, after collecting the male sperm and female ova, store them in the dark, and check the eggs and eggs under a dissecting mirror and microscope. Sperm screening, during the screening process, select transparent, plump, uniform-sized eggs and sperm with a long swimming time;

[0094] 2) Artificial insemination: Dilute the sperm with Ringer's solution, the volume ratio of the Ringer's solution to the sperm is 1:40 to obtain a sperm dilution, mix the sperm dilution with the egg, and the result is The volume ratio of the sperm diluent and the egg is 1:20, after stirring evenly, adding seawater to it for activation, the temperature of the seawater is 19°C, and the sperm-egg mixture activated by seawater is obtained;

[0095] 3) Cold shock treatment induces an...

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Abstract

The invention relates to an induction and detection method for androgenesis dihaploid of Paralichthys olivaceus. The induction and detection method provided by the invention has the beneficial effects that nuclear genetic materials in eggs can be deactivated by only carrying out cold-shock treatment on the fertilized eggs for a certain time, the effect the same as that achieved by ray irradiation deactivation in the traditional androgenesis induction techniques is achieved; and hydrostatic treatment is adopted, the androgenesis dihaploid of the Paralichthys olivaceus can be rapidly prepared.

Description

technical field [0001] The invention belongs to the technical field of aquatic organisms, and in particular relates to a method for inducing and detecting double haploids of flounder androgenesis. Background technique [0002] Flounder (Paralichthysolivaceus), belonging to the class Osteichthyes, order Pleuronectiformes, suborder Pleuronectoidei, family Paralichthyidae, genus Paralichthys, is a warm-temperate bottom ferocious marine fish , the size of the individual can reach more than 800mm. It has the characteristics of large individual, tender meat, full of fat and delicious taste, and easy digestion. At the same time, flounder has the characteristics of fast growth, strong reproductive ability, small migration and strong regression. , Japan and other countries important seawater aquaculture fish. [0003] The growth rate of male and female flounder is significantly different. In production, the breeding of whole female flounder can greatly increase the breeding yield an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 候吉伦
Owner 中国水产科学研究院北戴河中心实验站
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