35 results about "Regulatory molecules" patented technology

The invention provides a single molecule detection device and method based on an electrochemical gating technology in a quantum tunneling region, and relates to a detection technology for regulating molecular energy level alignment and regulating molecular conductance through gate voltage, a functionalized tunneling electrode is prepared at the tip of a nano pipettor and is placed in a solution containing single molecules, and the electrochemical gating technology in the quantum tunneling region is used for detecting the single molecules in the nano pipettor. The gate potential and the bias voltage are dynamically adjusted, tunneling current signals flowing through the single molecule are recorded and analyzed in real time, the structure, the charge state and the energy state of the single molecule can be actively regulated and controlled, and dynamic detection of the single molecule is achieved according to the conductivity change spectrum of the single molecule under different potential conditions. And the single molecule detection precision based on the tunneling electrode is obviously improved.

Embodiments of the invention include compositions and methods related to engineered human mucosal-associated invariant T (eMAIT) cells for off-the-shelf use for clinical therapy for cancer, infectious, and autoimmune diseases. In some embodiments, the eMAIT cells are produced from healthy human donor peripheral blood, cord blood, or G-CSF mobilized peripheral blood. In particular embodiments, the eMAIT cells are produced from a pluripotent stem cell line and therefore can be of unlimited supply. In some embodiments, the eMAIT cells are engineered to express chimeric antigen receptors (CARs), or / and immune regulatory molecules, or / and allorejection resistance molecules. Embodiments of the invention also include compositions of matter comprising polynucleotides encoding mucosal-associated invariant T cell receptor alpha chain polypeptides and / or mucosal-associated invariant T cell receptor beta chain polypeptides.

The invention relates to the technical field of medicinal chemistry, radiopharmaceutical chemistry and clinical nuclear medicine, in particular to a CDK4 / 6-targeted molecular imaging probe labeling precursor, a probe and a preparation method and application thereof. According to the molecular imaging probe [68Ga] Ga-PY03 provided by the invention, on the basis of a Ribociclib structure, a PEG1 chain and proline with envelope conformation are introduced, so that a hydrophobic region in a protein binding pocket can be filled, Van der Waals force can be enhanced, the molecular imaging probe [68Ga] Ga-PY03 can be stably bound with CDK4 / 6, and the targeting of the molecular imaging probe to the CDK4 / 6 is further ensured; the hydrophilicity of the molecular imaging probe can be adjusted, and kidney uptake is reduced; and the compound has excellent tumor / liver and tumor / muscle ratio, and can effectively improve tumor uptake.

The present application relates to the technical field of biological medicine, and in particular to a cat triple vaccine immune adjuvant containing two kinds of cat interleukins and metabolic regulatory molecules, a composition and application. The composition comprises cat interleukin 15, cat interleukin 23 and a metabolic regulatory molecule, the metabolic regulatory molecule comprises beta-nicotinamide mononucleotide, beta-nicotinamide adenine dinucleotide disodium salt, N-acetyl cysteine and L-carnitine, and can be used for preparing a cat triple vaccine or an auxiliary preparation of the cat triple vaccine. The composition can effectively improve the protection of the vaccine, prolong the protection time of the vaccine and reduce adverse reactions; the vaccine can more durably and at a higher level reduce or control the reproductive infection of viruses, significantly improve the specific humoral immune level and memory response ability of animals, and better resist the invasion and spread of FPV, FCV and FHV-1.

The invention relates to the technical field of biological medicine, in particular to a cat triple vaccine immunologic adjuvant containing two cat interleukinins and a metabolism regulating molecule, a composition and application. The composition comprises cat interleukin 15, cat interleukin 23 and metabolic regulation molecules, the metabolic regulation molecules comprise beta-nicotinamide mononucleotide, beta-nicotinamide adenine dinucleotide disodium salt, N-acetylcysteine and L-carnitine, and the composition can be used for preparing a cat triple vaccine or an auxiliary preparation of the cat triple vaccine. The composition can effectively improve the protection capacity of vaccines, prolong the protection time of the vaccines and reduce adverse reactions; the vaccine can reduce or control reproduction infection of viruses in a more durable and higher level mode, the specific humoral immunity level and the memory response capacity of animals are remarkably improved, and invasion and diffusion of FPV, FCV and FHV-1 are better resisted.

The invention discloses an application of glycosylated phosphatidylinositol specific phospholipase D1 (GPLD1) as a sperm-egg recognition and / or binding regulation molecule.

The technology described herein is directed to Aptamer and Ribozyme Equilibrium Shifting (ARES) regions, including ON-switches and OFF-switches, which can be harnessed to regulate the stability of RNA molecules. Also described herein are compositions comprising such RNA molecules and methods of using them to regulate translation of cargo polypeptides.

The application discloses a kind of big steric hindrance C-C coupling palladium ligand and synthesis method, ligand is used naphthyl as the main group of stable ligand, this group provides big plane rigid configuration during ligand use, simultaneously, it provides big π electron for complex molecule to stabilize complex;In addition, the configuration of pyridofuran is used, the N coordination point of traditional N-P bidentate ligand is introduced O by furan this functional group in ortho position to adjust the chemical environment of intramolecular N coordination point, so as to modify the traditional N-P bidentate ligand to tridentate ligand, this change can effectively increase the penetration ability of Pd active center in actual ligand use to improve catalytic activity.

The invention discloses a benzothiazole non-condensed ring A-D-A '-D-A type organic solar cell acceptor material and a preparation method and application thereof, a benzothiazole unit is used as a central nuclear electron withdrawing unit (A-unit), and then the benzothiazole unit, the central nuclear electron withdrawing unit (A-unit), an electron donating unit (D-unit) and a terminal group are synthesized into the organic solar cell acceptor material with an A-D-A'-D-A structure. Wherein benzothiazole as an asymmetric central core structure can enhance the dipole moment of molecules, optimize the accumulation among the molecules and improve the blending property of a donor and an acceptor, and the characteristics can jointly promote charge transfer to inhibit the bimolecular recombination condition. The molecular morphology is regulated and controlled by connecting different structural groups to a top branched chain, and meanwhile, different atoms can be introduced into a benzene ring to regulate and control the chemical energy level. In addition, the polymer also has the characteristics that the aggregation behavior and the light absorption range of molecules can be adjusted by accessing different terminal groups. The benzothiazole-based non-fused ring A-D-A '-D-A type acceptor material provided by the invention is few in synthesis and preparation steps, simple in purification process and high in yield.

This invention discloses the application of the CEMIP-ITGA5 axis as a target in the preparation of products that regulate epithelial-mesenchymal transition (EMT) and metastasis in lung adenocarcinoma, belonging to the fields of tumor molecular biology and precision medicine. Through TCGA-LUAD cohort bioinformatics analysis, immunohistochemical verification of clinical specimens, cell function experiments, and a nude mouse subcutaneous xenograft model, this invention discovers and confirms that the CEMIP-ITGA5 axis participates in regulating epithelial-mesenchymal transition, migration, invasion, and metastasis-related malignant progression in lung adenocarcinoma. Specifically, CEMIP positively regulates ITGA5 expression, thereby promoting EMT, proliferation, migration, invasion, and in vivo tumorigenesis in lung adenocarcinoma cells. Based on this, the present invention provides two applications: Firstly, by detecting the expression levels of the dual genes CEMIP and ITGA5, a kit for assessing the risk of epithelial-mesenchymal transition (EMT) and metastasis in lung adenocarcinoma can be prepared. The combined detection of CEMIP and ITGA5 can reflect the activation status of the CEMIP-ITGA5 axis at both the upstream regulatory molecule and downstream effector molecule levels, providing auxiliary molecular evidence for assessing EMT and metastasis-related risks in lung adenocarcinoma. Secondly, by targeting and inhibiting the expression or function of CEMIP and / or ITGA5, drugs can be prepared to inhibit epithelial-mesenchymal transition, migration, invasion, and metastasis-related malignant progression in lung adenocarcinoma, effectively reversing the epithelial-mesenchymal transition phenotype and inhibiting tumor malignant progression. This invention provides novel molecular targets and technical solutions independent of known signaling pathways for prognostic assessment and targeted therapy of lung adenocarcinoma.

The present invention discloses a multifunctional AIE optical diagnosis and treatment molecule and its azide compound, synthesis method and application. The multifunctional AIE optical diagnosis and treatment molecule uses triphenylamine as an electron donor and malononitrile fluorene as an electron acceptor. By adjusting the D-A structure of the molecule, a series of organic optical diagnosis and treatment molecules are synthesized, and a novel multifunctional optical diagnosis and treatment molecule with AIE properties is screened out. At the same time, further azide modification of the multifunctional optical diagnosis and treatment molecule can realize its covalent binding with functional groups such as polypeptides, DNA and small molecule compounds. The multifunctional AIE optical diagnosis and treatment molecule of the present invention has multifunctionality and can perform near-infrared fluorescence imaging, photodynamic therapy and photothermal therapy on tumors.

The present application relates to a kind of target protein conformation targeting regulatory molecules and regulatory methods in living cells.The protein conformation targeting regulatory molecules include: 1) with the targeting group of selectively binding target protein as skeleton, 2) two active functional groups are modified on the skeleton, c) rigid connecting arm introduced between targeting group and active functional group.Based on the above characteristics, after the protein conformation targeting regulatory molecules of the cell are incubated with cell, they are rapidly combined with target protein in cell specifically through cell membrane, and covalent reaction occurs with amino acid near binding pocket, to target the conformation of target protein is regulated.According to the difference of protein conformation and reaction site, different active groups and rigid connecting arm are used, to realize the accurate regulation of target protein conformation in different functional states.The present application provides important technical support in the field of exploring protein conformation and function, expanding the library of druggable proteins, researching new use of old drugs, disease treatment or prevention.

The present invention relates to the application of SOX13 as a gastric cancer biomarker. By constructing ferroptosis-resistant strains, the present invention uses a microarray to screen out SOX13 as an important regulatory molecule for gastric cancer ferroptosis sensitivity, and discovers that the anti-influenza drug zanamivir can specifically target the SOX13 protein. The present invention effectively verifies that the SOX13 gene is involved in the regulation of gastric cancer ferroptosis sensitivity, providing theoretical data for the ferroptosis sensitivity and chemosensitivity of gastric cancer cells. In addition, zanamivir can specifically target the SOX13 protein and reverse gastric cancer ferroptosis resistance, providing a theoretical basis for the treatment of ferroptosis resistance and chemoresistance of gastric cancer cells.

The present invention provides a single-molecule detection device and method based on electrochemical gating technology in the quantum tunneling region. It is a detection technology that regulates the alignment of molecular energy levels and adjusts molecular conductivity by regulating gate voltage. A functionalized tunneling electrode is prepared at the tip of a nanopipette and placed in a solution containing a single molecule. The gate potential and bias voltage are dynamically adjusted, and the tunneling current signal flowing through the single molecule is recorded and analyzed in real time. It can actively regulate the structure, charge state and energy state of the single molecule. According to the conductivity change spectrum of the single molecule under different potential conditions, dynamic detection of the single molecule is achieved, significantly improving the detection accuracy of single molecules based on tunneling electrode detection.

The technology described herein is directed to Aptamer and Ribozyme Equilibrium Shifting (ARES) regions, including ON-switches and OFF-switches, which can be harnessed to regulate the stability of RNA molecules. Also described herein are compositions comprising such RNA molecules and methods of using them to regulate translation of cargo polypeptides.

The invention relates to a downstream motion factor identification method and system for regulating brown fat metabolism and a storage medium, and relates to the technical field of bioinformatics, and the method comprises the following steps: (1) processing a sample and obtaining transcriptome original data; (2) difference analysis; (3) time sequence analysis; (4) enrichment analysis; (5) carrying out weighted gene co-expression network analysis; (6) machine learning; according to the method, downstream metabolism-promotion factors of brown adipocytes are identified by using RNA next-generation sequencing results, overall characteristics of transcriptomes are obtained through difference analysis, enrichment analysis and time sequence analysis, data are further reduced by using weighted gene co-expression network analysis and machine learning, and key regulatory genes are screened out; according to the invention, key regulation molecules capable of promoting metabolism of brown adipocytes can be found, and a new biological target is provided for treating obesity.

The present disclosure relates to methods and compounds for treating genetic diseases, in particular, compounds and methods for modulating the expression of dmpk, and treating diseases and conditions in which dmpk plays a positive role. The compound may be a transcriptional regulating molecule having a first end, a second end, and an oligomeric backbone, wherein: a) the first end comprises a DNA binding moiety capable of non-covalently binding to a nucleotide repeat CAG or CTG; b) the second terminal comprises a protein binding moiety that binds to a regulatory molecule that modulates the expression of a gene comprising the nucleotide repeat CAG or CTG; and c) the oligomeric backbone comprises a linker between the first end and the second end.

The invention discloses a naphthalimide-based light diagnosis and treatment molecule with a controllable self-assembly behavior as well as a preparation method and application thereof, and relates to the technical field of near-infrared two-region light diagnosis and treatment. According to the functionalized light diagnosis and treatment molecular library based on the naphthalimide skeleton, the problems that the aggregation-induced performance of a traditional light diagnosis and treatment agent is reduced, the assembly controllability is insufficient, the structural characteristics, the accumulation mode and the performance adjustment are not clear and the like are solved by adjusting the molecular structure, the accumulation mode and the light physical performance of the traditional light diagnosis and treatment agent; the method is suitable for the field of near-infrared two-zone light diagnosis and treatment in biomedicine, and aims to improve the deep tissue imaging and treatment effect.

Aspects of the present invention include methods and compositions related to the modulation of molecules regulating the regenerative potential of cells and tissues in the embryonic state and the loss thereof in later fetal and adult stages of development. Said methods and compositions have uses in research in stem cell biology and in increasing regenerative potential in fetal and adult tissues otherwise incapable of regeneration.

The technology described herein is directed to Aptamer and Ribozyme Equilibrium Shifting (ARES) regions, including ON-switches and OFF-switches, which can be harnessed to regulate the stability of RNA molecules. Also described herein are compositions comprising such RNA molecules and methods of using them to regulate translation of cargo polypeptides.

The invention provides a novel miRNA target for treating AIDS (acquired immune deficiency syndrome) infection and cervical cancer and application of the novel miRNA target. The miRNA comprises miR-H1 (micro Ribonucleic Acid), miR-TAR (micro Ribonucleic Acid) and miR-N367, and is called HIV1-miRNAs for short. The HIV1-miRNAs exist in a cervical cancer patient suffering from HIV (Human Immunodeficiency Virus) infection. The HIV1-miRNAs can participate in the occurrence and development process of HIV combined cervical cancer by regulating and controlling tumor key pathways such as cell proliferation and inflammation in cervical cancer cells. The invention also provides regulatory molecules (including antisense nucleotides) for targeted regulation. The invention provides a new diagnosis and treatment way for clinical treatment and detection of HIV combined cervical cancer.

The disclosure provides polypeptides, polynucleotides, compositions, kits and methods useful for modulating RNA molecules including degrading disease-causing RNA(s) or stabilizing RNA(s) to treat diseases.

The invention discloses a construction method of an aneuploid acute myeloid leukemia cell model, which comprises the following steps: integrating an shRNA (short hairpin Ribonucleic Acid) expression element which can be induced by doxycycline DOX to express a targeted SAC component BUB1B or CENPE into a genome of an AML (acute myeloid leukemia) cell line; dOX is added into a culture system for induction, and the cells can express shRNA targeting BUB1B or CENPE in a transient manner, so that the expression quantity of BUB1B or CENPE is knocked down, the normal function of SAC is mildly interfered, and enough survivable AML aneuploid cells are induced. The method disclosed by the invention has the advantages that firstly, a non-core regulatory molecule BUB1B or CENPE of the SAC is knocked down in a sexual manner, so that the function of the SAC is mildly interfered, and the cell survival rate in the induction process is guaranteed; the'aneuploid 'is used as a unique main anomaly, so that the phenomenon obtained by taking the cell line as a tool to research is determined to be caused by the'aneuploid' characteristic; the method has nuclear heterogeneity, and can better simulate the aneuploid state of AML cells clinically.

The invention discloses an application of Inobrodib in preparation of a medicine for treating lung adenocarcinoma, and the Inobrodib inhibits proliferation, migration and transfer ability of LUAD cells by down-regulating expression of key regulation and control molecules RBBP4 and P300. The invention provides a new thought and strategy for accurate treatment of LUAD, and has a clear target mechanism basis, a good drug intervention effect and a potential clinical conversion value.

The invention provides application of an LncRNANAV2-AS2 gene or an NAV2 gene in preparation of a medicine for treating atherosclerosis, and belongs to the technical field of biological medicines. According to the application disclosed by the invention, NAV2-AS2 plays a protective role in atherosclerosis, and NAV2 is one of downstream regulation molecules of the NAV2-AS2.

The present invention provides the use of TBRG4 as a drug target in in vitro screening for drugs for the treatment of gallbladder cancer, wherein the drug is a drug that inhibits TBRG4 expression. The present invention also provides the use of a reagent for detecting TBRG4 in the preparation of a kit for diagnosing gallbladder cancer. The research results of the present invention found that inhibiting TBRG4 can significantly inhibit the proliferation, migration and invasion of gallbladder cancer cells, suggesting that TBRG4 is an important regulatory molecule affecting the progression of gallbladder cancer. Further studies have shown that the U2AF2 / TBRG4 / EGFR signaling pathway can regulate the glycolysis process of gallbladder cancer cells through the PI3K / AKT pathway. Inhibiting TBRG4 can significantly inhibit the glycolysis of gallbladder cancer cells, thereby confirming that TBRG4 is an important energy metabolism regulatory molecule, opening up a new therapeutic approach for the treatment of gallbladder cancer.

The present application relates to the fields of medicinal chemistry, radiopharmaceutical chemistry and clinical nuclear medicine technology, in particular to a kind of molecular imaging probe targeting CDK4 / 6 labeled precursor, probe and preparation method and application thereof.The molecular imaging probe provided by the present application [68 Ga]Ga-PY03 is based on Ribociclib structure, by introducing PEG1 chain and proline with envelope conformation, not only can fill the hydrophobic region in protein binding pocket, enhance van der waals force, so that the molecular imaging probe [68 Ga]Ga-PY03 can be stably combined with CDK4 / 6, thereby ensuring the targeting of the molecular imaging probe to CDK4 / 6;It can also regulate the hydrophilicity of the molecular imaging probe, reduce kidney uptake;And excellent in tumor / liver and tumor / muscle ratio, can effectively improve tumor uptake.

Aspects of the present invention include methods and compositions related to the modulation of molecules regulating the regenerative potential of cells and tissues in the embryonic state and the loss thereof in later fetal and adult stages of development. Said methods and compositions have uses in research in stem cell biology and in increasing regenerative potential in fetal and adult tissues otherwise incapable of regeneration.

Provided are a method for epigenetically editing a target site and use thereof. In particular, provided are a method for regulating the expression level of a target gene, and a nucleic acid-binding molecule. The combination of the nucleic acid-binding molecule and a gene expression-regulating molecule has the capability of adjusting the expression level of the target gene.

Aspects of the present invention include methods and compositions related to the modulation of molecules regulating the regenerative potential of cells and tissues in the embryonic state and the loss thereof in later fetal and adult stages of development. The methods and compositions have uses in research in stem cell biology and in increasing regenerative potential in fetal and adult tissues otherwise incapable of regeneration.