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Hydroxyapatite hollow micro-carrier material and preparation method thereof

A technology of hydroxyapatite and carrier materials, applied in biochemical equipment and methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve the problems of difficult removal of force, unfavorable immersion, etc., and achieve dense and uniform HA. Good cell adhesion, low material density effect

Inactive Publication Date: 2012-07-11
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low density of hollow glass microspheres, it is easy to float on the upper layer of simulated body fluid, which is not conducive to complete immersion; moreover, the force generated by the collision of microspheres is not easy to remove.

Method used

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  • Hydroxyapatite hollow micro-carrier material and preparation method thereof
  • Hydroxyapatite hollow micro-carrier material and preparation method thereof
  • Hydroxyapatite hollow micro-carrier material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Take the hollow glass microspheres in the middle of the two meshes sieved with 100 mesh and 140 mesh sieves, then clean them with deionized water, and place them in a blast drying oven until dry after cleaning.

[0024] (2) Immerse the hollow glass microspheres cleaned and dried in step (1) in a 1mol / L NaOH solution, take them out after soaking for 2 hours under constant stirring, and take the complete microspheres and place them in a drying oven for drying. The dried hollow glass microspheres were immersed in a container containing 1.5×SBF solution of bioglass (the mass ratio of bioglass to hollow glass microspheres was 1:1), and placed in a constant temperature oscillator in a water bath, and the water temperature was adjusted to 36.5°C. The frequency is 80 times / min. After soaking for 2 days, take out and wash with deionized water and dry at room temperature.

[0025] (3) Immerse the hollow glass microspheres treated in step (2) into 1.5×SBF solution, put them i...

Embodiment 2

[0027] (1) Take the hollow glass microspheres in the middle of the two meshes sieved with 100 mesh and 140 mesh sieves, then clean them with deionized water, and place them in a blast drying oven until dry after cleaning.

[0028] (2) Immerse the hollow glass microspheres cleaned and dried in step (1) in a 1mol / L NaOH solution, take them out after soaking for 2 hours under constant stirring, and take the complete microspheres and place them in a drying oven for drying. The dried hollow glass microspheres were immersed in a container containing 1.5×SBF solution of bioglass (the mass ratio of bioglass to hollow glass microspheres was 0.2:1), and placed in a constant temperature oscillator in a water bath, and the water temperature was adjusted to 36.5°C. The frequency is 80 times / min. After soaking for 2 days, take out and wash with deionized water and dry at room temperature.

[0029] (3) Immerse the hollow glass microspheres treated in step (2) into 1.5×SBF solution, put them...

Embodiment 3

[0031] (1) Take the hollow glass microspheres in the middle of the two meshes sieved with 100 mesh and 140 mesh sieves, then clean them with deionized water, and place them in a blast drying oven until dry after cleaning.

[0032] (2) Immerse the hollow glass microspheres cleaned and dried in step (1) in a 1mol / L NaOH solution, take them out after soaking for 2 hours under constant stirring, and take the complete microspheres and place them in a drying oven for drying. The dried hollow glass microspheres were immersed in a container containing 1.5×SBF solution of bioglass (the mass ratio of bioglass to hollow glass microspheres was 0.2:1), and placed in a constant temperature oscillator in a water bath, and the water temperature was adjusted to 36.5°C. The frequency is 80 times / min. After soaking for 2 days, take out and wash with deionized water and dry at room temperature.

[0033] (3) Immerse the hollow glass microspheres treated in step (2) into 1.5×SBF solution, put them...

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Abstract

The invention relates to a hydroxyapatite hollow micro-carrier material and a preparation method thereof. The method comprises the following steps: immerging a cleaned and dried hollow glass microsphere into a NaOH solution and soaking under the continuous agitation; taking out the hollow glass microsphere, drying and immersing the hollow glass microsphere into a container containing 1.5*SBF (Simulated Body Fluid) solution of bioactive glass for soaking and activating at a constant temperature; and immerging the hollow glass microsphere into the 1.5*SBF solution for soaking and depositing at a constant temperature. The hydroxyapatite hollow micro-carrier material and preparation method thereof have the benefit that the obtained material is small in density, HA (Hyaluronic Acid) on the surface is compact and uniform, a coating is weakly-crystallized HA containing carbonate and has good functions of promoting cell adhesion, multiplication and differentiation, and the components of the coating are more close to human bones to facilitate the growth of cells; and meanwhile, the coating is not limited by matrix shape, and the process is simple and easy to operate.

Description

technical field [0001] The invention relates to a hydroxyapatite hollow microcarrier material, in particular to a hydroxyapatite hollow microcarrier material and a preparation method thereof, belonging to the technical field of biological materials. Background technique [0002] Microcarrier culture takes advantage of the large specific surface area of ​​the microsphere and the high utilization rate of the medium. The culture of cells on the surface of the microsphere can obtain a large number of cells in a short period of time, and basically avoid the damage of the cells during the trypsinization process. Therefore, microcarrier culture has become the most commonly used and most effective animal cell culture carrier so far. However, most of the currently used microcarrier materials are polymers, which can reach a high level of repeatability and mechanical properties, but lack cell recognition sites, which affect the adhesion and growth of cells on their surfaces. At the sa...

Claims

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Application Information

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IPC IPC(8): C03C17/22C12M3/02
Inventor 吕宇鹏焦燕肖桂勇
Owner SHANDONG UNIV
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