Method for rapid and indoor identification of broomrape resistance level of sunflowers
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A sunflower, rapid technology, applied in horticultural methods, botany equipment and methods, seed and rhizome treatment, etc., can solve the problems of long experimental period, slow germination of Liedang seeds, difficulty in observing the germination and parasitic process, etc., and achieve rapid induction , strong practicability, strong promotion and application value
Active Publication Date: 2017-03-22
INNER MONGOLIA AGRICULTURAL UNIVERSITY
View PDF4 Cites 11 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0003] Aiming at the deficiencies of the prior art, the present invention provides a method for rapidly inducing the germination of sunflower sunflowers under indoor conditions, that is, the culture dish filter paper method, which aims to solve the problem of slow germination of sunflower seeds, difficulty in observi
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Example Embodiment
[0016] Example 1
[0017] figure 1 A flow chart of a method for rapidly inducing sunflower rowan germination under indoor conditions provided by the present invention is shown, and the operation method of the specific steps of the method is as follows:
[0018] 1. Disinfection and pretreatment of sunflower Liedang seeds
[0019] 1.1. Cleaning of sunflower rowan seeds
[0020] The sunflower seeds collected from the field were passed through a sieve (diameter 0.18 mm) to remove impurities. Collect the sunflower columns from which impurities have been removed and put them into a 1.5ml centrifuge tube (1 / 3 volume), then add distilled water, and centrifuge at 12000r / min for 15 minutes. After discarding the supernatant, spread the precipitate on the filter paper, dry it naturally, sweep it down with a brush, and store it in a 1.5ml centrifuge tube for later use.
[0021] 1.2. Disinfection treatment of sunflower Liedang seeds
[0022] Add 1ml of 0.1% sodium hypochlorite (1.5ml c...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a method for rapidly inducing broomrape germination of sunflowers under indoor conditions, in particular to a culture vessel filter paper method and an application thereof to identification of the broomrape resistance level of the sunflowers. Broomrape seeds are induced to germinate on an indoor culture vessel, moisture is preserved by filter paper, the germination condition of the seeds can be directly observed by a microscope, culture is continued for 3 weeks, and broomrape parasitism wart structures of the sunflowers can be observed with naked eyes. Compared with a nutrition bowl method, the steps of sunflower planting and later fine root counting, operation is facilitated, observation is easily realized, and time is greatly saved. A system can be built to rapidly screen and indentify the broomrape resistance level of different sunflower varieties, and serves as a premise and a basis for broomrape resistance breeding of the sunflowers. The identification result of a culture vessel filter paper method is effectively consistent with a field test result, so that the broomrape resistance level of the different sunflower varieties can be rapidly identified.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly inducing germination of sunflower sangritang under indoor conditions, that is, the petri dish filter paper method, and its application in rapid identification of sunflower sangritangia level. Background technique [0002] Sunflower Liedang (Orobanche cumana Wallr), also known as poisonous root grass, is an annual herbaceous parasitic seed plant, belonging to dicotyledonous plants, Orobanche cumana Wallr, mainly damages sunflower, watermelon, melon, pea, broad bean, carrot, celery, tobacco, flax , tomato, etc. Sunflower Liedang is a major disease that seriously threatens the development of sunflower industry at present. It occurs in all sunflower producing areas in my country, but it occurs most seriously in Xinjiang and Inner Mongolia. After the sunflower is listed as parasitic, the sunflower plant is short and thin, unable to form a flower disc, and finally cause...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more