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Method for rapid and indoor identification of broomrape resistance level of sunflowers

A sunflower, rapid technology, applied in horticultural methods, botany equipment and methods, seed and rhizome treatment, etc., can solve the problems of long experimental period, slow germination of Liedang seeds, difficulty in observing the germination and parasitic process, etc., and achieve rapid induction , strong practicability, strong promotion and application value

Active Publication Date: 2017-03-22
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] Aiming at the deficiencies of the prior art, the present invention provides a method for rapidly inducing the germination of sunflower sunflowers under indoor conditions, that is, the culture dish filter paper method, which aims to solve the problem of slow germination of sunflower seeds, difficulty in observing the germination and parasitic process, and long experimental period. The problem of poor repeatability, at the same time, the establishment of a method for rapidly inducing the germination of sunflower rowang under indoor conditions can also be applied to the rapid identification of sunflower resistance to rowang

Method used

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  • Method for rapid and indoor identification of broomrape resistance level of sunflowers
  • Method for rapid and indoor identification of broomrape resistance level of sunflowers
  • Method for rapid and indoor identification of broomrape resistance level of sunflowers

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Embodiment 1

[0017] figure 1 A flow chart of a method for rapidly inducing sunflower rowan germination under indoor conditions provided by the present invention is shown, and the operation method of the specific steps of the method is as follows:

[0018] 1. Disinfection and pretreatment of sunflower Liedang seeds

[0019] 1.1. Cleaning of sunflower rowan seeds

[0020] The sunflower seeds collected from the field were passed through a sieve (diameter 0.18 mm) to remove impurities. Collect the sunflower columns from which impurities have been removed and put them into a 1.5ml centrifuge tube (1 / 3 volume), then add distilled water, and centrifuge at 12000r / min for 15 minutes. After discarding the supernatant, spread the precipitate on the filter paper, dry it naturally, sweep it down with a brush, and store it in a 1.5ml centrifuge tube for later use.

[0021] 1.2. Disinfection treatment of sunflower Liedang seeds

[0022] Add 1ml of 0.1% sodium hypochlorite (1.5ml centrifuge tube) to t...

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Abstract

The invention relates to a method for rapidly inducing broomrape germination of sunflowers under indoor conditions, in particular to a culture vessel filter paper method and an application thereof to identification of the broomrape resistance level of the sunflowers. Broomrape seeds are induced to germinate on an indoor culture vessel, moisture is preserved by filter paper, the germination condition of the seeds can be directly observed by a microscope, culture is continued for 3 weeks, and broomrape parasitism wart structures of the sunflowers can be observed with naked eyes. Compared with a nutrition bowl method, the steps of sunflower planting and later fine root counting, operation is facilitated, observation is easily realized, and time is greatly saved. A system can be built to rapidly screen and indentify the broomrape resistance level of different sunflower varieties, and serves as a premise and a basis for broomrape resistance breeding of the sunflowers. The identification result of a culture vessel filter paper method is effectively consistent with a field test result, so that the broomrape resistance level of the different sunflower varieties can be rapidly identified.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly inducing germination of sunflower sangritang under indoor conditions, that is, the petri dish filter paper method, and its application in rapid identification of sunflower sangritangia level. Background technique [0002] Sunflower Liedang (Orobanche cumana Wallr), also known as poisonous root grass, is an annual herbaceous parasitic seed plant, belonging to dicotyledonous plants, Orobanche cumana Wallr, mainly damages sunflower, watermelon, melon, pea, broad bean, carrot, celery, tobacco, flax , tomato, etc. Sunflower Liedang is a major disease that seriously threatens the development of sunflower industry at present. It occurs in all sunflower producing areas in my country, but it occurs most seriously in Xinjiang and Inner Mongolia. After the sunflower is listed as parasitic, the sunflower plant is short and thin, unable to form a flower disc, and finally cause...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01C1/00
CPCA01C1/00A01G7/06
Inventor 徐东升赵君张园园高婧石胜华李敏贾瑞芳周洪友田再民
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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