16results about How to "Shorten induction time" patented technology

The invention relates to the technical field of stem cell induced differentiation, and discloses an umbilical cord mesenchymal stem cell adipogenic induced differentiation culture medium and a culture method. The culture medium comprises the following substances: a DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium, fetal calf serum, insulin, penicillin, streptomycin, dexamethasone, 3-isobutyl-1-methylxanthine, indometacin, BMP-2 and FGF-1 (Fibroblast Growth Factor 1). The culture method comprises the following steps: (1) carrying out primary isolation culture on umbilical cord mesenchymal stem cells; (2) carrying out passage on the umbilical cord mesenchymal stem cells; and (3) inducing adipogenesis of the umbilical cord mesenchymal stem cells by using the adipogenesis induced differentiation culture medium. BMP-2 and FGF-1 are added on the basis of a traditional adipogenic induction culture medium to induce and promote the umbilical cord mesenchymal stem cells to be more efficiently differentiated into adipocytes under the combined action, so that the adipogenic differentiation time is shortened, and the adipogenic differentiation efficiency is improved; the umbilical cord mesenchymal stem cell adipogenic culture medium is stable in formula, simple and convenient in culture method and suitable for wide popularization.

The present invention relates to an induction medium and an expansion medium for inducing and amplifying TIL cells from tumor tissues, and a method for inducing and / or amplifying TIL cells. The induction medium comprises one or two selected from IL-7 and IL-15, IL-2, TNF-α, CD3 antibody and CD28 antibody. The expansion medium comprises one or two selected from IL-7 and IL-15, IL-2, TNF-α, CD3 antibody, CD28 antibody and CD137 antibody.

The invention belongs to the technical field of foam composite materials and application thereof in natural gas hydrates, and particularly relates to an amphiphilic composite foam material and a preparation method and application thereof. The preparation method of the amphiphilic composite foam material comprises the following steps: taking a precursor solution of polyimide and polytetrafluoroethylene as raw materials, foaming through an isocyanate method to obtain a polytetrafluoroethylene / polyimide foam material, and then carrying out alkali etching on the polytetrafluoroethylene / polyimide foam material to obtain the amphiphilic block type composite foam material. The preparation method of the amphiphilic composite foam material is low in raw material cost, simple in process, reliable in principle, high in recyclability and friendly to preparation environment, and the prepared foam material is stable in structure. The invention further provides application of the amphiphilic composite foam material in storage of the natural gas hydrate, and the amphiphilic structure distributed in a block mode can synergistically promote efficient and rapid generation of the natural gas hydrate.

The invention discloses a drug for constructing a venous inflammation model in the field of phlebitis model technology, comprising the following preparation steps: S1: preparing a carbomer hydrogel, comprising the following steps: step 1: putting 2.5g of carbomer and 19.5ml of pure water into a beaker and stirring to form a mixture; step 2: letting the mixture stand and stirring several times at intervals of 5 to 10 minutes; step 3: adding several drops of triethanolamine into the beaker; step 4: adding 5ml of pure water into the beaker, then adding 0.5ml of antibacterial agent, and then stirring; S2: injecting 25ml of 20% mannitol into the carbomer hydrogel and then standing; S3: injecting 1.5μl of TBHP stock solution into the carbomer hydrogel, and uniformly mixing the carbomer hydrogel with TBHP. The present invention uses carbomer hydrogel to uniformly mix mannitol and TBHP; and compared with injecting drugs into blood vessels, injecting into the perivascular area is not only easier to operate and has a high success rate, but also can avoid the drug from spreading to the whole body with the blood, causing systemic toxicity.

The invention discloses a double-opposed-piston rapid compressor ignition experiment device, which comprises a combustion system, which comprises a combustion chamber and pistons, two ends of the combustion chamber are provided with openings, the openings are connected with pipelines where the pistons are located, and the front opening of the combustion chamber is provided with a window; the air pressure system comprises an air pressure bin and a quick compressor, and the air pressure bin is of a double-opposed structure; the quick compressor can compress air to 10 atmospheric pressures; the sample introduction and exhaust system comprises a gas cylinder; a gas mixing tank; gas in the gas cylinder is filled into the gas mixing tank through a pipeline, the vacuum pump is connected with the combustion chamber and the gas pressure bin through an exhaust pipe and used for vacuumizing the combustion chamber and the gas pressure bin, and premixed gas is stored in the gas mixing tank and used for filling the combustion chamber with the premixed gas through a pipeline; the oil pressure system comprises an oil pressure bin and an oil pressure pump. According to the double-opposed-piston quick compressor ignition experiment device, an ignition system can be reasonably arranged, knocking is restrained, and combustion is fully carried out.

The invention discloses a fire-fighting spraying and fire barrier system for a high-rise building, and relates to the technical field of fire-fighting systems. The high-rise building fire-fighting spraying and fire barrier system comprises a water source, a water supply pipeline, a control device, a nozzle and a fireproof material, the control device comprises a smoke detector, an alarm valve group, a water flow indicator and a signal valve, and the smoke detector comprises a protective cover and a smoke sensor body arranged in the protective cover; and a pressurizing mechanism is arranged in the protective cover. According to the fire-fighting spraying and fire barrier system for the high-rise building, through the design of the pressurizing mechanism, the pressurizing mechanism can be started conveniently when the smoke sensor main body detects the smoke content and the smoke content does not reach a set threshold value, the airflow circulation speed is increased, and therefore nearby air is pressurized and actively sucked after smoke sensing, and the safety of the high-rise building is improved. The alarm can be triggered at the first time when the smoke is increased, and the sensitivity of the smoke sensor main body is improved.

The invention belongs to the field of carbon dioxide capture, and relates to an adsorbent, a preparation method and application thereof, and a carbon dioxide hydration adsorption method. The preparation method of the adsorbent comprises the following steps: S1, graphene oxide and a nitrogen-containing compound are subjected to a hydrothermal reaction in water, the condition of the hydrothermal reaction enables the graphene oxide to be doped with nitrogen and cut into quantum dots, and nitrogen-doped graphene oxide quantum dots are obtained; s2, the nitrogen-doped graphene oxide quantum dots, tetrabutylammonium fluoride and cyclopentane are mixed, and the adsorbent is obtained. The method is characterized in that the graphene oxide quantum dots are prepared by taking a nitrogen-containing compound as a cutting agent and a doping agent, and on the basis, the obtained nitrogen-doped graphene oxide quantum dots, tetrabutylammonium fluoride and cyclopentane are compounded to be used as a carbon dioxide hydration adsorbent, so that the separation efficiency of carbon dioxide can be effectively improved.

The invention provides a method for forming a regenerated plant through a direct generation way of a amorphophallus bulbifer somatic embryo, which comprises the following steps: collecting germinated non-opened Buddhist flame of amorphophallus bulbifer, taking a tender female inflorescence as an explant, screening and optimizing amorphophallus bulbifer female inflorescence to induce the direct generation, maturation and plant regeneration conditions of the somatic embryo, and obtaining the regenerated plant through the direct generation way of the amorphophallus bulbifer somatic embryo. And an efficient plant regeneration system is preliminarily established. Under proper temperature, illumination and culture conditions, the direct germination and seedling formation of the somatic embryo and the transplanting condition are good. The method provides a theoretical basis and a technical method for in-vitro preservation of germplasm resources of the amorphophallus bulbifer in the Hainan hot area, rapid propagation and large-area popularization of seedlings and construction of a genetic transformation receptor system regeneration system of the amorphophallus bulbifer, and is of great significance in assisting sustainable development of the amorphophallus bulbifer industry in the Hainan hot area.

The application discloses a method for promoting oil palm callus proliferation and somatic embryo induction by using baicalin. The tender yellow leaf of oil palm is used as a tissue culture explant, and Y3 medium is used as a basic medium, and baicalin is added to promote the induction and proliferation of oil palm callus and somatic embryo generation. In the callus induction and proliferation stage, 0.5 mg / L of baicalin can replace part of the effect of 2,4-D or dicamba, reduce the dosage of 2,4-D or dicamba, reduce the risk of subsequent regeneration deformity, shorten the callus induction time by 20 days, and increase the callus proliferation rate by 48.19%; in the somatic embryo induction stage, 0.3 mg / L of baicalin can completely replace the plant hormone 6-BA, and the somatic embryo induction time is shortened by 31 days, and the somatic embryo induction rate is increased by 5%. Therefore, the addition of baicalin in the medium can improve the efficiency of oil palm callus proliferation and somatic embryo induction, shorten the induction time, and has a good popularization and application prospect in the field of oil palm tissue culture and rapid propagation.

The invention discloses all-solid waste concrete. The all-solid waste concrete is prepared by mixing the following components in parts by mass: 350-500 parts of a cementing material, 980-1140 parts of coarse aggregate, 890-960 parts of fine aggregate and 120-160 parts of water, the cementing material comprises slag, stainless steel slag, fly ash, desulfurized gypsum and an exciting agent; the coarse aggregate comprises ferrochrome slag; the fine aggregate comprises ferronickel slag. According to the all-solid waste concrete disclosed by the invention, a solid waste material rich in a CaO-SiO2-Al2O3 system is selected as a cementing material, the cementing material is self-decomposed under the action of water and reacts to generate C-S-H gel and ettringite, and the ettringite and the flocculated C-S-H gel are tightly interwoven to form a compact structure, so that the strength of the concrete can be remarkably improved at the early stage of hydration of the concrete; implementation verifies that the all-solid-waste concrete can meet the requirements of C40-grade concrete at most, all-solid-waste materials are 100% adopted, the problem of environmental pollution caused by solid waste stockpiling can be solved, and meanwhile regenerated products with high additional value are formed.

The utility model relates to the technical field of fire-fighting early warning, and solves the problem that fire-fighting early warning can be carried out only after a fire occurs for a period of time because the floating speed of smoke is slow and the time for sensing the smoke by a probe is long, so that the best emergency rescue time can be taken. The fire-fighting early warning device comprises a box body, a mounting disc is arranged in the box body, an alarm module used for smoke sensing alarm is arranged at the top of the mounting disc, a smoke sensor used for sensing smoke is arranged at the bottom of the mounting disc, and a negative pressure assembly used for absorbing the smoke is arranged at the bottom of the box body. The negative pressure assembly comprises a smoke suction box arranged at the bottom of the box body, the top of the smoke suction box is fixedly connected with a fixing disc, a motor groove is formed in the middle of the surface of the fixing disc, the interior of the motor groove is fixedly connected with a driving motor, and the output end of the driving motor is fixedly connected with a negative pressure fan used for sucking smoke.

The invention relates to the technical field of biomedical detection, and relates to a preparation method and application of oxidized low-density lipoprotein. The preparation method of the oxidized low-density lipoprotein comprises the following steps: low-density lipoprotein is subjected to high-frequency ultrasonic treatment under the conditions of 380-430 kHz and 30-45 W, the ultrasonic temperature is 25 DEG C, the ultrasonic time is 5-20 min, and the oxidized low-density lipoprotein is obtained. The method has the advantages of being rapid, efficient and environmentally friendly, the oxidation time is remarkably shortened, and meanwhile the oxidation effect is kept.

The invention discloses a method for promoting oil palm callus proliferation and somatic embryo induction by utilizing baicalin. Oil palm tender yellow leaves are used as tissue culture explants, a Y3 culture medium is used as a basic culture medium, and baicalin is added to promote oil palm callus induced proliferation and somatic embryogenesis. Wherein 0.5 mg / L of baicalin is added in the callus induction proliferation stage, the effect of part of 2, 4-D or dicamba can be replaced, the dosage of 2, 4-D or dicamba is reduced, the risk of subsequent regeneration malformation is reduced, the callus induction time is shortened by 20 days, and the callus proliferation rate is increased by 48.19%; 0.3 mg / L of baicalin is added in the somatic embryo induction stage, so that plant hormone 6-BA can be completely replaced, meanwhile, the somatic embryo induction time is shortened by 31 days, and the somatic embryo induction rate is increased by 5%. Therefore, by adding the baicalin into the culture medium, the callus proliferation and somatic embryo induction efficiency of the oil palm can be improved, the induction time is shortened, and the method has good popularization and application prospects in the field of tissue culture and rapid propagation of the oil palm.

The invention relates to the field of sensor humidity detection, and discloses a rice processing technology and a detection and distribution device which comprises a humidity-dependent sensor and a pressure sensor. By arranging the humidity-dependent sensor, the humidity of the rice is detected, if the humidity of the rice reaches the standard, the rice can be separated to the next working procedure for screening and impurity removal, if the humidity of the rice does not reach the standard, the rice can be separated to the drying box for re-drying, and it is ensured that the rice with the humidity not reaching the standard is dried to reach the standard state; whether the humidity of the rice is qualified or not is judged through the humidity-dependent sensor and the pressure sensor, the humidity can be detected in real time, material distribution can be conducted, the speed is high, and efficiency is high.

The invention belongs to the technical field of synthesis of alkylphenol polyether, and particularly relates to a CoFe DMC-coated PmPD composite catalytic material as well as a preparation method and application thereof, and a preparation method of alkylphenol polyether. The preparation method comprises the following steps: injecting a hexacyanoferrate (III) acid salt mixed solution into a cobalt salt mixed solution, mixing, and aging to obtain a CoFe DMC material; the injection speed is greater than or equal to 100 [mu] L.min <-1 >, and the aging time is greater than or equal to 24 h; and mixing the CoFe DMC material, m-phenylenediamine, a free radical initiator and water, and performing free radical polymerization reaction on the surface of the CoFe DMC material to obtain poly (m-phenylenediamine), thereby obtaining the CoFe DMC-coated PmPD composite catalytic material. When the CoFe DMC-coated PmPD composite catalytic material prepared by the invention is used for preparing alkylphenol polyether, the induction time can be shortened by more than half, and the CoFe DMC-coated PmPD composite catalytic material has obvious guiding significance of reducing cost and improving efficiency in industrial production of alkylphenol polyether products.

The invention provides a method for forming a regenerated plant by a direct somatic embryogenesis pathway of a pearly konjac, collecting the germination of unopened spathe flames of a pearly konjac class yellow konjac, using tender female inflorescences as explants, screening and optimizing the female inflorescences of the pearly konjac to induce direct somatic embryogenesis, maturation and plant regeneration conditions, and preliminarily establishing an efficient plant regeneration system. Under suitable temperature, light and culture conditions, the somatic embryo directly germinates into seedlings and transplants well. The invention provides a theoretical basis and technical methods for the in vitro preservation of germplasm resources of pearly konjac in Hainan hot zone, rapid breeding of seedlings and large-scale promotion, and the construction of a genetic transformation receptor system regeneration system of pearly konjac, which is of great significance to the sustainable development of the pearly konjac industry in Hainan hot zone.