1results about How to "Shorten induction time" patented technology

The application discloses a method for promoting oil palm callus proliferation and somatic embryo induction by using baicalin. The tender yellow leaf of oil palm is used as a tissue culture explant, and Y3 medium is used as a basic medium, and baicalin is added to promote the induction and proliferation of oil palm callus and somatic embryo generation. In the callus induction and proliferation stage, 0.5 mg / L of baicalin can replace part of the effect of 2,4-D or dicamba, reduce the dosage of 2,4-D or dicamba, reduce the risk of subsequent regeneration deformity, shorten the callus induction time by 20 days, and increase the callus proliferation rate by 48.19%; in the somatic embryo induction stage, 0.3 mg / L of baicalin can completely replace the plant hormone 6-BA, and the somatic embryo induction time is shortened by 31 days, and the somatic embryo induction rate is increased by 5%. Therefore, the addition of baicalin in the medium can improve the efficiency of oil palm callus proliferation and somatic embryo induction, shorten the induction time, and has a good popularization and application prospect in the field of oil palm tissue culture and rapid propagation.