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2014results about How to "Broaden your options" patented technology

Method for obtaining temperature-sensitive sterile line by performing site-specific mutagenesis on P/TMS12-1 through CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system

The invention discloses a method for obtaining a temperature-sensitive sterile line by performing site-specific mutagenesis on P/TMS12-1 through a CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system. The method comprises the following steps: cloning and controlling a Pei'ai 64S temperature-sensitive sterile major gene P/TMS12-1 fragment; designing a target sequence according to the P/TMS12-1 sequence; constructing a pU3-gRNA carrier of the target-containing sequence fragment; constructing a pCRISPR/Cas9 carrier; obtaining a positive transgenic seedling by utilizing the pCRISPR/Cas9 carrier containing the target sequence fragment; screening a mutant plant from the positive transgenic seeding; performing subculture planting on the mutant plant to obtain the temperature-sensitive sterile line without transgenic components. According to the method disclosed by the invention, the CRISPR/Cas9 system is utilized to completely inactivate P/TMS12-1 non-coding RNA, and the temperature-sensitive sterile line without transgenic components is artificially cultivated. The method disclosed by the invention has the advantages of being strong in purposiveness, small in genome damages and capable of avoiding transgenic interference.
Owner:SOUTH CHINA AGRI UNIV

3D printing device suitable for multi-material workpieces

The invention discloses a 3D printing device suitable for multi-material workpieces. The 3D printing device comprises three-dimensional moving structures, a workbench, a rotary plate type sprayer switching device, a feeding system and a control system. The three-dimensional moving structures comprise the X-axis moving structure, the Y-axis moving structure and the Z-axis moving structure. The workbench is fixedly connected with the X-axis moving structure and is driven by the X-axis moving structure and the Y-axis moving structure to perform horizontal motion and driven by the Z-axis moving structure to perform vertical motion. The rotary plate type sprayer switching device comprises a rotary plate and a motor; multiple segmented screw rod sprayer devices are mounted on the rotary plate in the circumferential direction, and the rotary plate is driven by the motor to rotate for switching. The feeding system is used for providing pulp for the segmented screw rod sprayer devices. The control system is used for controlling the movement of the three-dimensional moving structures and the rotary plate and extrusion of the pulp in the segmented screw rod sprayer devices. According to the 3D printing device, the selecting range of processing materials is expanded, and the 3D printing device has the advantages of being high in work efficiency, low in production cost and the like, and can effectively reduce material hysteresis and flowing phenomena.
Owner:HUAZHONG UNIV OF SCI & TECH

Intelligent disinfecting equipment

Intelligent disinfecting equipment is an ultralow-volume, intelligent and three-dimensional disinfecting device composed of a medicine applying device, a central control device, an external interaction device, a walking device, a power supply device and a rack. When 5-100 micrometers of ultramicro medicine particles containing effective disinfecting components are conveyed to a disinfecting environment through an airflow conveying device of the disinfecting device, the disinfecting operation process is completed under cooperation of the central control device containing a motion control module, a data module and a task management module, the external interaction device and the power supply device; preparation, analysis and comparison of whole disinfecting operation, environmental meteorological data acquiring and automatic collection, recording, storage and transmission of data of operation control and the operation process can be achieved through the Internet of Things and cloud computing in the disinfecting process; disinfection, management and monitoring are timely and are synchronized and integrated. The intelligent disinfecting equipment saves medicine, water and labor, and automation, standardization, formulation and menulizing of the disinfecting operation can be achieved.
Owner:北京中知领见科技有限公司
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