192 results about "Excitation spectra" patented technology

A molecular construct capable of fluorescent resonance energy transfer (FRET), comprising a linker peptide, a donor fluorophore moiety and an acceptor fluorophore moiety, wherein the linker peptide is a substrate of a botulinum neurotoxin selected from the group consisting of synaptobrevin, syntaxin and SNAP-25, or a fragment thereof capable being cleaved by the botulinum neurotoxin, and separates the donor and acceptor fluorophores by a distance of not more than 10 nm, and wherein emission spectrum of the donor fluorophore moiety overlaps with the excitation spectrum of the acceptor fluorophore moiety; or wherein the emission spectra of the fluorophores are detectably different. Also provided are isolated nucleic acid expressing the construct, kits comprising said construct and cell lines comprising said nucleic acid. Further provided are methods of detecting a BoNT using the above described construct via FRET, and methods for detecting a BoNT using surface plasmon resonance imaging.

Apparatus for determining the oxygenation state of at least one tissue element, comprising: a) illumination means for illuminating said tissue element with an illuminating radiation at a predetermined wavelength via at least one illumination location with respect to said tissue element, said illuminating radiation being at a wavelength within the NADH excitation spectrum or the Fp excitation spectrum; b) radiation receiving means and detection means for measuring the intensity of the corresponding NADH fluorescence or Fp fluorescence emitted by the tissue element at at least two predetermined wavelengths within the range of wavelengths comprised within the corresponding fluorescence emission spectrum.

The present invention is directed to a monitor system that measures flight characteristics of at least one object moving in a predetermined field-of-view using at least one fluorescent marker. In one embodiment, the emission spectra of the fluorescent marker is preferably narrow and substantially symmetric. It may be desirable for the fluorescent marker to be capable of responding to a broad excitation spectra. Preferably, the fluorescent markers comprise quantum dots. The quantum dots may be manufactured in any desired manner, and may comprise semiconductors, gold atoms, and the like.

The invention has for its object the provision of an oxynitride fluorescent material has higher emission luminance than conventional rare earth element-activated sialon fluorescent materials. To this end, an oxynitride fluorescent material is designed in such a way as to contain as the primary constituent a JEM phase represented by a general formula MA1(Si6−zAlz)N10−zOz wherein M is one or two or more elements selected from the group consisting of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu. For instance, this fluorescent material has a fluorescent spectrum maximum emission wavelength of 420 nm to 500 nm inclusive and an excitation spectrum maximum emission excitation wavelength of 250 nm to 400 nm inclusive.

This invention discloses an Excitation Emission Matrix (EEM) fluorescence spectrometer system that uses an LED array to cause excitation emission matrix fluorescence that is imaged onto a spectrograph for sample identification and analysis. By using the LED array, the spectrograph requires only high spectroscopic resolution of about 1 to 5 nm in the fluorescence emission range and low excitation light resolution of about 14 to 73 nm in the excitation range. While individual LED optical excitation spectra may contain wavelength regions that overlap, as long as the various LEDs have different excitation wavelengths and intensities, spectral overlap does not preclude data analysis. The invention provides an EEM spectroscopy system that is not a high resolution such as a laser or lamp based excitation system, but instead uses a lower resolution, lower power LED system. The invention also provides results that are comparable to existing systems with lower component cost and lower power requirements while also optically stable, small, and easy to use.

The invention discloses a preparation method of functional fluorescence carbon nanoparticles (CNPs) based on natural saccharide materials and representation and application thereof in measurement for biotechnique. The preparation method is characterized by comprises the following steps of dissolving 1g of carbon sources (cellulose, starch, chitosan and cyclodextrin) and 0.1g of NaOH into 15ml of deionized water, then reacting reacting for 4 hours in an autoclave at 160 DEG C, and carrying out acid-base neutralization and microwave treatment on the obtained carbon nanoparticles. According to the preparation method, the raw materials are abundant and cheap and are easily available, the preparation method is simple, and the synthesized nanoparticles have good fluorescence characteristic and the characteristics of stable fluorescence, dark flashing, tunable wavelength, wide excitation spectra, narrow emission spectrum and the like, and can be used as a fluorescence indicator in the biological medicine field.

A phosphor converted light emitting device includes a semiconductor structure comprising a light emitting layer disposed between an n-type region and a p-type region, the light emitting layer being configured to emit light having a first peak wavelength; a first phosphor configured to emit light having a second peak wavelength; and a second phosphor configured to emit light having a third peak wavelength. The second phosphor is an Eu3+-activated phosphor, configured such that in the excitation spectrum at 298K and 1.013 bar, a maximum intensity in a wavelength range between 460 nm and 470 nm is at least 5% of a maximum intensity in a wavelength range between 220 nm to 320 nm.

A phosphor converted light emitting device includes a semiconductor structure comprising a light emitting layer disposed between an n-type region and a p-type region, the light emitting layer being configured to emit light having a first peak wavelength; a first phosphor configured to emit light having a second peak wavelength; and a second phosphor configured to emit light having a third peak wavelength. The second phosphor is an Eu3+-activated phosphor, configured such that in the excitation spectrum at 298K and 1.013 bar, a maximum intensity in a wavelength range between 460 nm and 470 nm is at least 5% of a maximum intensity in a wavelength range between 220 nm to 320 nm.

The invention provides an LED package. The LED package at least comprises a support, a wafer, weld lines and a sealing component, wherein the support is used for supporting the wafer and the weld lines, the wafer is used for emitting light capable of effectively exciting fluorescent powder or quantum dots, the weld lines are used for connecting the wafer and the support, the quantum dots are distributed in the sealing component, and the sealing component is located in a path of light emitted from the wafer, is in the shape of a lens and is used for wrapping the wafer and the support. The sizes of the quantum dots can be changed to control emission spectra of the quantum dots; the LED package has very good stability; because wide excitation spectra and narrow emission spectra are provided, the color rendering index of the LED package is effectively improved; the LED package is high in light emitting efficiency.

The present invention concerns fluorescent proteins modified such that said modified fluorescent protein incorporates a cleavage site for a protease, cleavage of said modified fluorescent protein at said cleavage site by said protease causing the alteration of at least one of the emission and excitation spectra of said modified fluorescent protein. In particular, the invention is concerned with using these modified fluorescent proteins as probes for detecting protease activity in living cells during the programmed cell death process (apoptosis).Also provided are nucleic acid sequence encoding same, recombinant DNA constructs expressing same, cells transformed or transfected with same, methods for detecting protease activity, and methods of detecting agents which affect protease activity, and kits for same.

One or more excitation energy sources emit light in an excitation spectrum and direct the emitted light as an excitation beam to the emitting surface of a wavelength conversion element directly or via reflection. Distinct areas of the emitting surface are coated with one or more distinct fluorescent phosphors. The phosphor-coated areas receive the excitation beam and generate a sequence of fluoresced light beams at a light output, each fluoresced beam of a narrow spectrum determined by the type of phosphor and the excitation spectrum. The fluoresced beams travel parallel to an emitting axis at a non-zero angle to axes associated with the excitation beams.

An object of the present invention is to provide a light emitting device which is high in emission intensity and stable, that is to say, a light emitting device in which when an LED or LD having an emission peak at 380 nm to 410 nm is used as an excitation light source of the light emitting device, the emission intensity of a red phosphor does not largely change to some deviation of the emission wavelength of the LED or LD to maintain not only brightness but also a balance at the time when mixed with a blue and green phosphors. The present invention relates to a light emitting device characterized in that the device comprises a phosphor which has Eu3+ as a luminescent center ion, in which a minimum emission intensity within the excitation wavelength range of 380 nm to 410 nm in an excitation spectrum is 65% or more of a maximum emission intensity, and which has an emission efficiency at 400 nm of 20% or more, and a semiconductor light emitting element which emits light in the region from near-ultraviolet light to visible light.

The invention relates to a vanadate garnet type fluorescent material as well as a preparation method and the application thereof, belonging to the field of vanadate inorganic luminescent materials and aiming at solving the technical problem of providing a wideband-emission fluorescent material with wide excitation spectrum range and high luminescent strength. The vanadate garnet type fluorescent material comprises the components: A(2-2x)K(1+x)NxM2V3O12, wherein x is more than or equal to 0 and less than or equal to 0.1, A is selected from at least one of Ca, Sr and Ba, M is selected from at least one element of Mg and Zn, and N is selected from at least one element of Ce, Pr, Sm, Nd, Eu, Gd, Tb, Dy, Ho, Er, Tm and Bi. The fluorescent material belongs to a garnet-type structure in a cubic system and has high luminescent strength and wide excitation spectrum band, and an emission spectrum is emitted by a wideband for covering the whole visible light region. The invention can be widely used in systems of electron, information, traffic, energy sources and illumination and has more remarkable superiority in the application of fourth-generation solid luminescent devices excited by purple light or long-wave ultraviolet light emitted by purple light or ultraviolet light-emitting diodes.

In another aspect, the present invention relates to a method for NMR measurements of an arbitrarily shaped region of interest of a living subject. In one embodiment, the method comprises the steps of applying a broad bandwidth of RF pulses to the arbitrarily shaped region of interest to obtain a corresponding spectrum, wherein substantially entire range of chemical shifts in the spectrum is excited from the arbitrarily shaped region of interest, interleaving a plurality of radial k-lines in radial k-space per excitation with non-selective refocusing pulses and obtaining spatial localization for the spectrum of the arbitrarily shaped region of interest.

The method for discriminating between benign and malignant prostate tumors relates to analyzing samples of blood, urine and tissue by fluorescence spectroscopy in order to detect the presence of naturally occurring molecules in the fluids and tissue that serve as biomarkers indicative of cancer in the human body. The analysis can be carried out based on fluorescence emission spectra, fluorescence excitation spectra and synchronous (emission and excitation) spectra of bio-samples. The detection, diagnosis, and follow-up and also discrimination between malignant and benign prostate tumors may be made by comparison of ratios of fluorescence emissions and / or excitation intensities of tryptophan, tyrosine, elastin, collagen, bile pigments, NADH, flavins and various species of porphyrins.

The present invention provides systems and methods for analyzing the excitation spectra of fluorescent particles in a flowing stream. The system uses a white light laser and color separation optics to provide a spatially-distributed, continuous color-spectrum excitation light system that is used to illuminate a region of a flowing stream. A particle that passes through the detection region traverses the full dispersed spectrum of excitation light, and the fluorescence emissions from the particle are continuously measured as it passes through the detection region. The measured fluorescence emissions at each wavelength of excitation light, which changes through full spectrum of the excitation light as the particle passes through the detection region, provides the excitation spectrum of the particle.

The current invention provides a persistent phosphor blend, along with techniques for making and using the blend. The persistent phosphor blend is made of at least one persistent phosphor combined with at least one other phosphor, where the excitation spectra of the one or more other phosphors overlap the emission spectra of the one or more persistent phosphors. The choice of the phosphors used allows the decay time and emission colors to be tuned for the specific application. In another embodiment, the invention provides a method for making persistent phosphor blends with tunable colors. In yet another embodiment, applications for such a persistent phosphor blend are provided.

A method of normalizing an analyzer response value of a fluorescence analyzer is provided. The method includes measuring an excitation spectrum of the analyzer and measuring an emission sensitivity spectrum of the analyzer. Next, a normalization factor based at least in part upon the excitation spectrum of the analyzer and the emission sensitivity spectrum of the analyzer is determined. The sample is then analyzed to obtain an uncorrected analyzer response value. A normalized analyzer response value is calculated based at least in part upon the uncorrected analyzer response value and the normalization factor.

The invention discloses a Mn<4+>-doped red light-emitting material and a preparation method thereof as well as a novel lighting source, and belongs to the field of a preparation method of fluorescent materials. The light-emitting material has a structural formula A[14-y]B6C[10-x]O35:xMn<4+>,yM<3+>, wherein in the formula, A represents one or two of alkaline-earth metal Ca, Sr or Ba; B represents one or two of Zn and Mg; C represents one or more of Al, Ga or In; M<3+> represents Sc, Y, Pr, Nd, Sm, Gd, Tb, Dy, Ho, Er, Tm, Yb or Lu; x is greater than or equal to 0.005 and less than or equal to 1; y is greater than or equal to 0 and less than or equal to 2. The invention further provides the preparation method of the Mn<4+>-doped red light-emitting material and the obtained novel lighting source. The Mn<4+>-doped red light-emitting material can be used for emitting red light in a wavelength range within 650nm to 750nm when being excited by excitation light sources such as ultraviolet light, near ultraviolet light or blue light.

A spherical phosphor includes a fluorescent substance having a maximum excitation wavelength of 400 nm or longer; and a transparent material containing the fluorescent substance, in which, in an excitation spectrum, an excitation spectral intensity in a wavelength region of from 340 nm to 380 nm is 50% or higher of an excitation spectral intensity at a maximum excitation wavelength. A wavelength conversion-type photovoltaic cell sealing material includes a light-permeable resin composition layer that contains the spherical phosphor and a sealing resin.

The invention discloses a method for preparing a high-power white LED with low color temperature and high color rendering property. In the method, fluorescent powder is excited by adopting an InGaN-based blue LED chip, wherein the fluorescent powder is a mixture of yellow fluorescent powder, green fluorescent powder and orange red fluorescent powder; both peak values of excitation spectra of the yellow fluorescent powder and the green fluorescent powder are matched with an emission spectrum of the InGaN-based blue LED chip; and the peak value of the emission spectrum of the green fluorescent powder is matched with the excitation spectrum of the orange red fluorescent powder. The yellow fluorescent powder and the green fluorescent powder are excited by the emitted light of the InGaN-based blue LED chip respectively, and the orange red fluorescent powder is excited by the emitted light of the green fluorescent powder, so that the method improves the photoluminescence conversion efficiency of the fluorescent powder. By reasonably regulating the ratio of the fluorescent powder, the method can ensure that the spectrum of the white LED consists of blue light, yellow light, green light and orange red light and has high continuity and high balance, and high luminous efficiency and the high color rendering property of the high-power white LED at a low color temperature are realized.

The invention discloses a tumor microvascular imaging instrument and a tumor microvascular imaging method. The tumor microvascular imaging instrument comprises an infrared confocal imaging part and a control part, wherein the infrared confocal imaging part comprises a near-infrared laser, a dichroscope, a two-dimensional scanning galvanometer, a scanning lens system, an imaging objective, a near-infrared fluorescence filter, a convergent lens, a pinhole and a detector; the scanning lens system is composed of a field lens and a cylindrical lens; the control part comprises a two-dimensional scanning control module which is used for controlling the two-dimensional scanning galvanometer, a signal amplification and acquisition module and a data processing and image displaying module; near-infrared quantum dots that a fluorescence excitation spectrum ranges from 1000nm to 1350nm and a fluorescence emission spectrum ranges from 1350nm to 1500nm are injected to blood vessels of a to-be-observed living body; and then a tumor part of the living body is observed under the tumor microvascular imaging instrument. According to the tumor microvascular imaging instrument and the tumor microvascular imaging method provided by the invention, complete three-dimensional imaging of tumor microvessels of the living body can be implemented with a high resolution.

A print medium is encoded with information bearing indicia. A transparent layer or substrate which receives the printed information during printing has a first surface, a second surface and a leading edge. A strip of tape is applied to the first surface. A machine readable information bearing indicia is applied to at least one surface of the tape strip. The indicia is formed by a pattern of fluorescent material which upon excitation by radiation of a given spectral excitation range emits radiation in a fluorescent spectral range. The tape reflects radiation in the given excitation spectral range and in the fluorescent spectral range. One type of indicia is a bar code pattern, although other types can also be employed.

The present invention is a method and system for reducing contamination in the resulting plasma of a weld produced by a fiber laser. The invention establishes the fiber laser in an optimal configuration for applying a high density beam to a weld material that eliminates spectral interference. The beam is applied in a narrow bandwidth of 1064 nm+ / −0.5 nm in one operative condition using an inert shielding gas, preferably argon, in a cross-flow or controlled environment around the welding region to prevent contamination of the plasma forming in the weld region. The method is optimized by determining and avoiding the emission spectrum for the fiber laser and the cover gas or gasses as well as any particular excitation spectra for the weld material. The system can utilize a single laser input, or can utilize multiple lasers joined by coupling means and utilizing a switch to select one or more of the fiber lasers.

The method for lung cancer detection by optical analysis of body fluids relates to analyzing samples of blood, urine and sputum by fluorescence spectroscopy in order to detect the presence of naturally occurring molecules in the fluids that serve as biomarkers indicative of cancer in the human body. The analysis can be carried out based on fluorescence emission spectra, fluorescence excitation spectra and synchronous (emission and excitation) spectra of bio-samples. The early detection and diagnosis of lung cancer may be made by comparison of ratios of fluorescence emissions and / or excitation intensities of tryptophan, tyrosine, elastin, collagen, bile pigments, NADPH, flavins and various species of porphyrins.

The invention relates to a method for the separation of molecules having different excitation spectra, which form components of a gas. The molecules are excited by laser pulses in a way that the molecules to be separated are transferred into a state of excitation due to multi-absorption of energy quanta from laser pulses, and in which they are extracted from the gas so that they exist in a composition determined by the form of the laser pulses. According to the invention, the laser pulses are formed by an iterative process in which each laser pulse varies in its form depending on the extracted molecules' composition after their absorption of energy quanta.

The invention belongs to the organic chemical field, and particularly relates to a lanthanide metal ether complex and preparation method and application thereof. The lanthanide metal ether complex, has the chemical structure equation shown in equation (I): (img file = 'DDA0001109726700000011.TIF' wi = '478' he = '663' / ). The invention designs a novel lanthanide metal ether complex, through selecting a designated substituent, enabling the prepared lanthanide metal ether complex to obtain an excitation spectrum excited using a wave length of 340nm fixed emission light, the intensity is relatively strong (reaching 400000), the florescent life is relatively long (larger than 0.2ms), the stability is relatively strong, therefore the complex has relatively good application prospect in fields of time resolution energy transfer and the like; the lanthanide metal ether complex has relatively short route, simple operation steps, and relatively high produce rate.