14 results about "Surface plasmon resonance imaging" patented technology

The invention discloses a surface plasmon resonance imaging system for expanding a dynamic range through a multi-mode optical path, and relates to the technical field of microscopic imaging, a laser outputs a monochromatic and linearly polarized continuous wave beam, a first dynamic beam deflection device performs dynamic angle deflection adjustment on the continuous wave beam, a prism is used for providing a total reflection interface, and a second dynamic beam deflection device is used for performing dynamic angle deflection adjustment on the continuous wave beam. Exciting an evanescent wave; a surface plasma resonance effect is generated through interaction of the SPR detection chip and evanescent waves, and sample characteristics are detected; the flow cell provides a sample flow channel, so that a sample is in full contact with the surface of the SPR detection chip; the second dynamic light beam deflection device cooperatively adjusts the propagation direction of reflected light on the surface of the SPR detection chip, and the imaging detection device captures a high-spatial-resolution SPR image formed by the reflected light and transmits the image to the upper computer in real time for processing and analysis. According to the invention, the change of the resonance angle can be tracked in real time, and high-sensitivity and high-resolution monitoring of the dynamic process of biomolecule interaction is realized.

The application discloses a wavelength modulation surface plasmon resonance imaging sensing method, which generates multiple groups of illumination spectra by controlling a light-emitting diode, processes the spectra into p-polarized light, excites a sensing chip to generate surface plasmon resonance through prism coupling, collects corresponding reflected light intensity images by using a CMOS camera, and reconstructs surface plasmon resonance reflection spectra of each pixel on the sensing chip by using a smooth constraint reconstruction algorithm according to intensity observation values of the pixel in all images and corresponding illumination spectrum data, so as to further extract a phase signal and generate a spatially resolved phase image. The phase signal and the imaging scheme realize high-sensitivity and high-throughput sensing performance. The method realizes low-cost, high-signal-to-noise ratio and high-throughput sensing imaging.

The application provides a DNA tetrahedron-based sensing chip, and also provides a preparation method and device thereof. The DNA tetrahedron-based sensing chip is prepared by modifying the surface of the DNA tetrahedron-based sensing chip with antibody functionalization modification. The DNA tetrahedron-based sensing chip prepared by the application can detect Alzheimer's disease through surface plasmon resonance imaging technology, has the advantages of high throughput, high sensitivity, and reusable chip, and the method shows good disease diagnosis performance. In the case that there is currently a lack of early detection means for Alzheimer's disease, the application selects a blood-based detection method and provides a miniaturized device for early detection and non-invasive detection of Alzheimer's disease.

A multi-modal surface plasmon resonance detector for measuring molecular interactions is provided herein. The multi-modal surface plasmon resonance detector includes a detector frame manufactured by metal additive manufacturing, the detector frame comprising integrated conformal cooling passages. The multi-modal surface plasmon resonance detector includes a prism providing a plurality of optical faces for a plurality of optical paths. The multi-modal surface plasmon resonance detector includes a first detection path configured for surface plasmon resonance detection. The multi-modal surface plasmon resonance detector includes a second detection path configured for surface plasmon resonance imaging. The multi-modal surface plasmon resonance detector includes a sensor chip interface configured to receive a sensor chip. The multi-modal surface plasmon resonance detector includes a liquid cooling system configured to circulate liquid through the integrated conformal cooling passages.

The invention relates to the field of polypeptide detection, in particular to a polypeptide and application thereof. One aspect of the invention provides a polypeptide comprising one or two of the amino acid sequences as shown in SEQ ID NOs: 1-2. The polypeptide provided by the invention is a high-affinity and high-specificity probe obtained by establishing a high-flux probe library through a mixed splitting method and utilizing magnetic bead primary screening and surface plasmon resonance imaging fine screening.

The invention discloses a method for high-throughput determination of antibody affinity based on combination of an antibody chip and surface plasmon resonance imaging. The method comprises the following steps: placing the antibody chip in a surface plasmon resonance imaging instrument, determining and outputting an affinity measurement result; the antibody chip is mainly formed by combining a photo-crosslinking chip and an antibody; the antibody comprises a plurality of antibody molecules, and in the antibody chip, the antibody is combined with the photo-crosslinking chip through chemical bonding. According to the scheme, the affinity value of the antibody combined with the antigen can be obtained on a large scale by circulating the antigen with gradient concentration, and the method for detecting the affinity of the antibody has the advantages of simplicity in operation, accurate detection result, low cost and stable performance.

The invention relates to the technical field of temperature control in a cell SPR (Surface Plasmon Resonance) imaging process, in particular to a device for controlling the temperature of a buffer solution to be constant in an imaging process and a use method. After the surface temperature of the chip and the temperature of the buffer solution are constant through the temperature control device, the SPRM experiment is executed, and the cells can be kept to be combined with and dissociated from drug molecules at the constant temperature. On the basis of the surface plasma resonance imaging technology, a buffer solution heating and refrigerating temperature control device is designed, on one hand, the temperature of the environment where cells are located in the SPRM experiment process is more constant, and therefore noise generated by cell environment temperature changes is reduced, and the influence of baseline drift on the experiment is reduced; on the other hand, a new application direction is developed, namely, the interaction between cells and drug molecules and the like under the environment temperature or even in the low-temperature state in the SPRM experiment is developed.

The application discloses a kind of diazirine compound base and preparation method and application thereof, it is related to microarray immobilization compound technical field.The diazirine compound base is obtained by using diazirine compound to modify polylysine base, it is simple in synthesis step, wide in application range, can be applied to high-throughput biochip preparation field, biological molecule interaction research, biological molecule activity evaluation, disease diagnosis, high-throughput screening, drug screening, fluorescence array imaging and surface plasmon resonance imaging etc., with wide market prospect;Meanwhile, when preparing biochip by diazirine compound base, preparation result is more reliable, and the detection result of prepared biochip is more sensitive, with low background and good signal, with universality, and the preparation method of biochip has the characteristics of simple operation, flexible control, without special equipment, low cost, high work efficiency.

A method for the spatiotemporal mapping of receptor-ligand binding kinetics in localized surface plasmon resonance (LSPR) imaging using a chip for LSPR imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array. The nanostructures can be regenerated allowing the chip to be used multiple times.

The application belongs to the field of biochemical substance detection, and particularly relates to a surface plasmon resonance imaging device and a refractive index detection method thereof. The surface plasmon resonance imaging device adopts a wavelength scanning device to realize switching of incident light wavelength, a voltage-adjusted transmittance device is used to compensate for transmittance deviation of a polarizer under different incident light wavelengths, an acquisition control system controls an applied voltage of the voltage-adjusted transmittance device and a wavelength of an output light beam of the wavelength scanning device, and simultaneously acquires data of an array detector under different wavelengths to perform differential operation and differential operation of average reflection light intensity of a sample point region and a sample point background region. The surface plasmon resonance imaging device and the refractive index detection method thereof reduce operation difficulty in the detection process, reduce operation time and equipment development cost.

The invention discloses a surface plasmon resonance imaging chip and a preparation method thereof. The surface plasmon resonance imaging chip comprises a substrate, a chromium coating, a nickel coating and a silver coating, wherein the chromium coating, the nickel coating and the silver coating are sequentially stacked on the substrate. The preparation method comprises the following steps: providing a substrate; preparing a chromium plating layer on the substrate; preparing a nickel plating layer on the chromium plating layer; and preparing a silver plating layer on the nickel plating layer. The three-layer metal material combination of the chromium plating layer, the nickel plating layer and the silver plating layer is used for carrying out surface plasma resonance imaging detection, and high biochemical detection sensitivity is achieved; and meanwhile, post-treatment is conducted on the plating layer through electrostatic laminating, deoxidizing agent placing and vacuumizing, so that the plating layer is high in stability and easy to store, the property of the plating layer can still be kept stable after the plating layer is stored at the room temperature for 6 months or above, and the cost is low.

The invention provides an SPR and SPRi target fishing method, and belongs to the technical field of biological chips. The preparation method comprises the following steps: fixing small molecules on an SPR (i) chip in a photo-crosslinking manner by a tiling method or a dot matrix printing method; putting the SPR (i) chip fixed with the small molecules into a corresponding surface plasma resonance (imaging) instrument, introducing a sample solution, carrying out target fishing, and recovering a regeneration solution; carrying out concentration, liquid change and enzymolysis on the regenerated liquid or carrying out in-situ degradation on an SPR (i) chip after target fishing by adopting trypsin to obtain a peptide fragment; and detecting the peptide fragment through LC-MS to determine the target protein in the sample solution. According to the target fishing method, small molecules do not need to be marked, the detection speed is high, the sample consumption is low, and active ingredients of natural products can be accurately detected and target acting protein can be accurately confirmed.

The present application is applicable to the field of surface plasmon resonance technology and provides a surface plasmon resonance imaging device, method, control system, and storage medium. The surface plasmon resonance imaging device includes a light source system, a detection system, a sample injection system, an imaging system, and a control system. The light source system includes a telecentric light source, a first motor, and an angle modulation component. The detection system includes a coupler and a microarray chip. The sample injection system includes a sample injection pool. The imaging system includes a first array detector. The first motor is mechanically connected to the angle modulation component, and the control system is electrically connected to the first motor and the first array detector, respectively. The control system is used to control the first motor to drive the angle modulation component to move, thereby adjusting the telecentric angle of the incident light generated by the telecentric light source so that the intensity of the incident light at all sample points on the microarray chip is equal, eliminating the need for measurement steps such as standard sample calibration or standard light intensity calibration, thereby meeting rapid detection requirements.

This invention discloses a device and method for real-time detection of single-nanoparticle protein crowns. The device includes a surface plasmon resonance imaging (SPRM) device comprising an optical module, a sample reaction unit, and a signal acquisition and analysis module. The SPRM device includes a laser emission module for heating single nanoparticles. This invention integrates the SPRM device with a laser emission module capable of heating single nanoparticles, achieving precise temperature control of single-particle protein crowns and enabling real-time, label-free, high spatiotemporal resolution detection of the protein crown formation process. Furthermore, this invention provides a high-precision experimental platform for quantitatively studying the thermodynamic processes of protein-nano interfaces and provides important theoretical and technical support for the design of nanomedicine carriers and biointerface engineering.