84 results about "Surface plasmon resonance imaging" patented technology

A molecular construct capable of fluorescent resonance energy transfer (FRET), comprising a linker peptide, a donor fluorophore moiety and an acceptor fluorophore moiety, wherein the linker peptide is a substrate of a botulinum neurotoxin selected from the group consisting of synaptobrevin, syntaxin and SNAP-25, or a fragment thereof capable being cleaved by the botulinum neurotoxin, and separates the donor and acceptor fluorophores by a distance of not more than 10 nm, and wherein emission spectrum of the donor fluorophore moiety overlaps with the excitation spectrum of the acceptor fluorophore moiety; or wherein the emission spectra of the fluorophores are detectably different. Also provided are isolated nucleic acid expressing the construct, kits comprising said construct and cell lines comprising said nucleic acid. Further provided are methods of detecting a BoNT using the above described construct via FRET, and methods for detecting a BoNT using surface plasmon resonance imaging.

An optical analysis unit especially suitable for performing grating coupled surface plasmon resonance (SPR) imaging features a pivoting light source capable of scanning through a range of angles of incident light projected onto a stationary target sensor, such as an SPR sensor. The reflected image from the illuminated sensor is detected, e.g., by a CCD camera and the image and angular scan data are processed, for example by a fitting algorithm, to provide real time analysis of reactions taking place on the surface of the sensor.

The invention relates to a preparation method of nanostructured array chip with surface plasma for resonance imaging. The preparation method is characterized by comprising the following steps: (1) selecting and cleaning a substrate; (2) using a vacuum evaporated coating to coat a first metal film on the substrate; (3) self-assembling a layer of polystyrene nanoparticles on the metal film; (4) coating a second metal film in a way of evaporation on the self-assembled layer so as to fill the gas between balls; (5) eliminating the nanoparticles with the Lift off technology; (6) using laser direct writing machine to manufacture a lattice mask plate of the level of micron; (7) adopting the photolithography to transmit the mask figure to the substrate; (8) acquiring the lattice figure through developing and stripping of photoresist; (9) using a chemical method to eliminate the excessive metal and photoresist; thus the metal lattice chip of the cyclical nanostructure can be formed. The method adopts the polystyrene nanoparticles for self-assembly so as to prepare the cyclical nanostructure, and thus is suitable for massive production of the nanostructure.

The invention relates to a surface plasmon resonance imaging sensing system, which comprises a plasma resonator with isosceles triangle cross-section, wherein the plane of the bottom side of the isosceles triangle is of a sensing plane, and a layer of metal reflection film is plated on the sensing plane; a sample pool with an inlet and an outlet is arranged on the metal reflection film, and one of the planes of the two sides of equal length is used as an incident plane, and the other thereof is used as an emittance plane; a power source, a beam shaper and a polarizer are sequentially arranged on an incident light path which is vertical to the incident plane to reach the sensing plane, and an analyzer, an imaging lens group and an area array photoelectric sensor are sequentially arranged on a reflection light path generated by the sensing plane; and the area array photoelectric sensor is positioned on a focal plane of the imaging lens group and is connected with an external computer. The surface plasmon resonance imaging sensing system is characterized in that: the light source is a non-coherent light source, and a narrow-band filter is arranged between the polarizer and the incident surface. The invention can be widely used in food safety detection, pesticide residue detection, bio-molecule detection, bio-terrorist detection and other fields.

A high-sensitivity SPR (surface plasmon resonance) sensor includes at least a prism having a first surface on which a metallic layer and a metallic nanoparticle layer are sequentially formed. A light source projects an incident light into the prism through a second surface of the prism. The light is reflected by the metallic layer and the metallic nanoparticle layer and leaves the prism through a third surface of the prism. A light detector detects the reflected light. The SPR sensor has an extensive detection range as compared with the conventional ones and is applicable in the detection of gas, chemical substance, and biomolecule. Moreover, the SPR sensor is advantageous in arranging fabrication process consistently, controlling film thickness, improving product quality, and decreasing fabrication cost.

This invention multifunction imager is made based on the surface plasma resonance phenomenon composed of p-polarized parallel light sources, a light incident angle modulation system, a sample transport and analysis system, a reflection light cut-away and modulation system, a detector, and a data register and process system. Said device utilizes the dispersion of P-polarized light and a prism to activate the resonance of sensing film surface plasma to test resonance signals. The resonance image signal or wave length scan resonance signal can be tested by conversion of light sources, the chip research result can be reached by micro-lattice process to sense film to realize spectrum conversion of image and resonance wavelength.

Disclosed are methods for making surface plasmon resonance-capable arrays wherein molecules, such as proteins or nucleic acids, or cells, are adhered to a metal substrate. The metal substrates are modified by depositing an omega-modified alkanethiol monolayer to the substrate and then contacting the omega-modified monolayer with a heterobifunctional linking compound. Biomolecules or cells can then be attached to the heterobifunctional linking compound. Also disclosed are arrays wherein glutathione-containing molecules are immobilized on the substrate and GST-containing molecules are then specifically immobilized onto the substrate, taking advantage of the affinity between glutathione and GST.

A surface plasmon resonance imaging apparatus provides an improved optical assembly allowing fixed source and detector operating with a horizontal test surface for a more compact design. In a preferred embodiment, a mechanical linkage of planar mirrors provides a single point adjustment of angle of incidence and angle of refraction while maintaining a constant optical axis of the source and detector.

Provided is a surface plasmon resonance sensor including: a part of delivering light by which a signal beam is incident to generate an evanescent field; and a part of exciting surface plasmon for exciting surface plasmons by the generated evanescent field and giving rise to a surface plasmon resonance, wherein a dielectric waveguide layer is inserted between metal layers of the part of exciting surface plasmon, and surface plasmon resonance properties are changed by an object to be analyzed.

A surface plasmon resonance sensor comprising a chip having a substrate (102) and a metal layer (103), a prism (104), an optical system (105) becoming a light source, and a photodetector (106), wherein the metal layer (103) consists of a flat part (109) in the form of a thin film, and protrusions consisting of fine metal particles (110) or the like arranged at intervals. When light impinges on such a metal layer (103), a resonance angle caused by the flat part (109) and each protrusion is attained. Variation in the refractive index of a medium touching the metal layer can be detected from the resonance angle.

The linear light beam scanned surface plasma resonant imaging light intensity detection method and system belongs to the field of biological detection technology. In the incident optical path, one vibrating mirror driven with one vibration and oscillation driver is set to alter the incident angle to the sensing surface and to complete linear light beam scanning. When the biomolecules in the surface of the protein chip act mutually, the vibration mirror reflected light radiates to the sensing surface in constantly changed angle to excite the surface plasma on the protein chip to resonate constantly, so as to obtain corresponding light intensity signal with the photoelectronic detector. Once the vibration mirror frequency is fixed, the detector signal is recorded to complete the detection. The present invention has simple system structure, high sensitivity, high precision and capacity of multichannel detection.

The invention relates to a sensor unit for a Surface Plasmon Resonance (SPR) unit, comprising a transparent sensor structure forming at least one wall of a cavity, the wall being defined by a concave inner surface and a convex outer surface The inner surface is provided with a layer of a conductive material capable of supporting a surface plasmon. In the cavity there is provided a flow structure in said cavity so as to form at least one compartment for sample between the flow structure and the inner wall of the cavity. Also, a method for the detection of events at a surface by utilizing surface plasmon resonance is provided. It comprises placing a sample with an analyte of interest in a sensor unit as claimed in claim 1, and measuring the reflectance from said sensor unit at a single or plurality of angle / angles.

The invention aims to provide a method for preparing surface plasma resonant imaging gold film micro-array. The method comprises the following steps: firstly self-assembling a gold size nanometer particle monolayer with the diameter of 2.5 nanometer on a glass plate modified by 3-Aminopropyltrimethoxysilane, taking the monolayer as a catalytic template; then taking a transparent film high-precision printed lattice array as a mask to carry out photo-etching to the glass plate modified by the gold nanometer particles, thereby preparing the micro-array of the gold nanometer particles; and controlling the gradual increase of the gold nanometer particle micro-array within the nanometer scale range by adopting the electroless plating technique, thereby constructing the required gold film micro-array. The size and the interval of each gold point in the micro-array can be conveniently controlled by the mask, and the thickness of the gold point is controlled by adjusting the electroless plating time.

The invention discloses a functional polymer thin film, a preparation method of the thin film and an application of the thin film. The preparation method comprises the following steps: (1) modifying a photo-cross-linking agent on the surface of a substrate; (2) rotationally coating the surface formed in the step (1) with a polymer solution; (3) performing irradiation reaction on the polymer solution with ultraviolet lights and covalently grafting the polymer to the substrate to form the polymer thin film; and (4) further functionalizing the polymer or directly performing biological sensing application. According to the preparation method, a three-dimensional polymer surface can be simply, quickly and efficiently prepared; the density and thickness of the surface polymer can be precisely controlled by regulating and controlling the density of the photo-cross-linking agent, the molecule weight of the polymer and spin-coating parameters; moreover, the preparation method is strong in operability, excellent in repeatability and suitable for mass production. The surface of the prepared polymer thin film has the high requirement for the fixed amount of bio-molecules and the excellent anti-nonspecific adsorption ability, thus the detection performance of a biological sensor (for example, surface plasmon resonance imaging, fluorescence, quartz crystal microbalance and electrochemical transducer) can be improved.

Disclosed are an apparatus and a method for detecting a surface plasmon resonance. The apparatus may radiate a polarized beam of light to a device, such as a surface plasmon resonance generator, and may measure variations in a temperature of the device to detect whether a surface plasmon resonance has occurred. The detection of a surface plasmon resonance may be associated with the presence of a particular material such as volatile organic compounds (VOCs) or materials disrupting ozone, for example.

There is provided a surface plasmon resonance imaging sensor capable of performing absolute calibration comprising: a transparent substrate; a first prism and a second prism formed at one surface of the substrate and symmetrically positioned with reference to the center axis of the substrate; an optical system for providing light to the first and second prisms; and a light receiving part for detecting the light reflected from the substrate, wherein a surface plasmon resonance (SPR) angle change of an object to be measured by the first prism is measured, and a refractive index change on each pixel of the object is obtained as a two-dimensional difference image by the second prism.

The invention discloses a microscopic imaging technique compounding surface plasma resonance (SPR) and surface enhanced Raman scattering (SERS) and relates to the field of surface plasma elements and surface enhanced Raman scattering. The technical key point disclosed by the invention is as follows: an SPR-SERS comprehensive microscopic imaging system is assembled, and SPR and SERS are excited and detected by a nano slit array raster bi-model structure; an SPR system judges change of an exciting angle by measuring movement of an SPR peak position to determine change of the surface effective refractive index induced by a biomolecular reaction on the surface of a chip; the SERS system directly differentiates the biomolecules by measuring the Raman spectrum; efficient and high-sensitive detection of SPR and Raman is dependent on the nano slit array raster bi-model structure: the excitation wavelength efficiently excites SPR through the raster structure; SPR generates dipole vibration in nanogaps in a metal surface spreading process, and the surface electric field generated by SPR and the nano silt dipole jointly act to enhance the surface local electric field to acquire an enhanced Raman signal.

Surface plasmon generation on a metal or semiconductor layer at an outer surface of an optical waveguide, using light reflected or scattered from inside the optical waveguide. One aspect provides a main optical waveguide (11) (e.g. optical fibre) having a second optical waveguide (18) adhered thereto, the second optical waveguide including an optically transparent material (610) separating two surface plasmon supporting layers (600, 620). Another aspect provides a surface plasmon supporting layer of material(s) adhered to the main optical waveguide, the layer having photo-induced regions of material compaction. The regions of compaction may cause un-inscribed refractive index modulations in the main optical waveguide. The surface plasmons are coupled to the guided mode(s) in the main optical waveguide. Surface plasmon resonance depends on sample material in contact with an outermost surface plasmon supporting layer. Properties of the sample material can thus be detected in output guided mode(s) because of the coupling with the generated surface plasmons.

Provided is a multi-channel surface plasmon resonance sensor using beam profile ellipsometry; and, more particularly, to a high sensitive measuring technology, which is coupled with a vertical illumination type focused-beam ellipsometer using a multi-incident angle measurement method, and a surface plasmon resonance (SPR) sensing part deposited with a metal thin film. The multi-channel surface plasmon resonance sensor includes a vertical illumination type focused-beam ellipsometer, in which light is polarized; a surface plasmon resonance (SPR) sensing part which is provided at the objective lens part of the focused-beam ellipsometer so as to generate SPR according to an angle change of the polarized light; and a flow unit which supplies a buffer solution containing a bio material binding to or dissociation from the metal thin film generating surface plasmon, wherein the SPR and the ellipsometric phase change by change in an angle and a wavelength are simultaneously detected.

A resonant image of using color light to excite surface plasma type consists of light source, modulation unit of incoming light angle, sample analysis unit, temperature control unit, detection unit, data collection and management unit. It is featured as applying composite-color light as excitation light source, using total reflection media-coupling to excite surface plasma of sensing film and to resonate with excited surface plasma for generating color image correlating to property of test sample.

The invention relates to a micro-cavity array mass spectrum target plate as well as a manufacturing method and application thereof. The micro-cavity array mass spectrum target plate comprises a substrate, an adhesion layer formed on the substrate and a conducting layer formed on the adhesion layer, wherein the side with the conducting layer of the micro-cavity array mass spectrum target plate is provided with a micro-cavity region consistent with a surface plasma resonance imaging detection region; micro-cavities are etched in the substrate in the position of the micro-cavity region, are opened on the conducting layer and are arranged into an array. The micro-cavity array mass spectrum target plate provided by the invention can be used for screening and enriching anode targets in a one-bead one-compound peptide library and directly carrying out in-situ substrate-assisted laser desorption ionization and flight time mass spectrometric detection on the anode targets; in addition, the micro-cavity array mass spectrum target plate can be combined with surface plasma resonance imaging to carry out in-situ affinity identification, so that the efficient and high-throughput screening and detection on the peptide library can be realized.

Described is a biointerface using near-infrared quantum dots for surface plasmon resonance imaging biosensors.

Its method includes the following steps: changing the beam emitted by broadband light source into a hairline beam; making said hairline beam be irradiated on SPR generation device; after the beam outcome from said device is dispersed, utilizing two-dimensional photoelectric detection device to make detection and obtain SPR spectrum information in a lighted line-type region of tested plane; translating said device and using said hairline beam to scan the whole tested plane to obtain SPR spectrum information of all the columns of whole tested plane; utilizing computer to make operation and processing to obtain refractive index information of whole tested plane in said device so as to form a two-dimensional image. Said method can be used for detecting various biological chips.