6 results about "Surface plasmon resonance imaging" patented technology

The application provides a DNA tetrahedron-based sensing chip, and also provides a preparation method and device thereof. The DNA tetrahedron-based sensing chip is prepared by modifying the surface of the DNA tetrahedron-based sensing chip with antibody functionalization modification. The DNA tetrahedron-based sensing chip prepared by the application can detect Alzheimer's disease through surface plasmon resonance imaging technology, has the advantages of high throughput, high sensitivity, and reusable chip, and the method shows good disease diagnosis performance. In the case that there is currently a lack of early detection means for Alzheimer's disease, the application selects a blood-based detection method and provides a miniaturized device for early detection and non-invasive detection of Alzheimer's disease.

A multi-modal surface plasmon resonance detector for measuring molecular interactions is provided herein. The multi-modal surface plasmon resonance detector includes a detector frame manufactured by metal additive manufacturing, the detector frame comprising integrated conformal cooling passages. The multi-modal surface plasmon resonance detector includes a prism providing a plurality of optical faces for a plurality of optical paths. The multi-modal surface plasmon resonance detector includes a first detection path configured for surface plasmon resonance detection. The multi-modal surface plasmon resonance detector includes a second detection path configured for surface plasmon resonance imaging. The multi-modal surface plasmon resonance detector includes a sensor chip interface configured to receive a sensor chip. The multi-modal surface plasmon resonance detector includes a liquid cooling system configured to circulate liquid through the integrated conformal cooling passages.

The application discloses a kind of diazirine compound base and preparation method and application thereof, it is related to microarray immobilization compound technical field.The diazirine compound base is obtained by using diazirine compound to modify polylysine base, it is simple in synthesis step, wide in application range, can be applied to high-throughput biochip preparation field, biological molecule interaction research, biological molecule activity evaluation, disease diagnosis, high-throughput screening, drug screening, fluorescence array imaging and surface plasmon resonance imaging etc., with wide market prospect;Meanwhile, when preparing biochip by diazirine compound base, preparation result is more reliable, and the detection result of prepared biochip is more sensitive, with low background and good signal, with universality, and the preparation method of biochip has the characteristics of simple operation, flexible control, without special equipment, low cost, high work efficiency.

A method for the spatiotemporal mapping of receptor-ligand binding kinetics in localized surface plasmon resonance (LSPR) imaging using a chip for LSPR imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array. The nanostructures can be regenerated allowing the chip to be used multiple times.

The application belongs to the field of biochemical substance detection, and particularly relates to a surface plasmon resonance imaging device and a refractive index detection method thereof. The surface plasmon resonance imaging device adopts a wavelength scanning device to realize switching of incident light wavelength, a voltage-adjusted transmittance device is used to compensate for transmittance deviation of a polarizer under different incident light wavelengths, an acquisition control system controls an applied voltage of the voltage-adjusted transmittance device and a wavelength of an output light beam of the wavelength scanning device, and simultaneously acquires data of an array detector under different wavelengths to perform differential operation and differential operation of average reflection light intensity of a sample point region and a sample point background region. The surface plasmon resonance imaging device and the refractive index detection method thereof reduce operation difficulty in the detection process, reduce operation time and equipment development cost.

This invention discloses a device and method for real-time detection of single-nanoparticle protein crowns. The device includes a surface plasmon resonance imaging (SPRM) device comprising an optical module, a sample reaction unit, and a signal acquisition and analysis module. The SPRM device includes a laser emission module for heating single nanoparticles. This invention integrates the SPRM device with a laser emission module capable of heating single nanoparticles, achieving precise temperature control of single-particle protein crowns and enabling real-time, label-free, high spatiotemporal resolution detection of the protein crown formation process. Furthermore, this invention provides a high-precision experimental platform for quantitatively studying the thermodynamic processes of protein-nano interfaces and provides important theoretical and technical support for the design of nanomedicine carriers and biointerface engineering.