Micro-cavity array mass spectrum target plate as well as manufacturing method and application thereof
A microcavity and array technology, applied in screening applications, in the field of microcavity array mass spectrometry target plates, can solve the problems of limited screening capacity, low screening efficiency, and high cost, and achieve the effect of high-throughput screening and detection
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Embodiment 1
[0052] Example 1: Fabrication of Microcavity Array Mass Spectrometry Target Plate
[0053] Such as figure 1 As shown, the microcavity array mass spectrometer target plate 1 provided in this embodiment includes a substrate 2, an adhesive layer 3 formed on the substrate 2, and a conductive layer 4 formed on the adhesive layer 3, so The side of the microcavity array mass spectrometry target plate 1 with the conductive layer 4 is provided with a microcavity area ( figure 1 The area where the array is located), the microcavity 5 is etched on the substrate 2 at the position of the microcavity area, and the microcavity 5 is opened in the conductive layer 4 and arranged in an array. For calibration needs, a standard sample calibration microcavity 6 may also be included.
[0054] Such as figure 2 As shown, the microcavity 5 is designed as a cube (the top view of the microcavity 5 is shown in the figure), the side length of which is 250 μm, and the distance between adjacent microcav...
Embodiment 2
[0069] Example 2: Using the microcavity array mass spectrometry target plate made in Example 1 to screen the polypeptide library
[0070] (1) Construction of peptide library
[0071] The nonapeptide fragment HA of the hemagglutinin protein was selected as the model polypeptide (sequence YPYDVPDYA). The HA antibody AHA was selected as the receptor protein that could specifically bind to it, and a model octapeptide library containing HA was established. The peptide library construction method uses amino-modified TentaGel (swellable particle size can be 200 microns) resin as a solid phase carrier, and before the first amino acid is synthesized, methionine is first coupled at the C-terminal. The Fmoc synthesis strategy was used for mixed and evenly divided synthesis, and the four amino acids Y, P, D, and V constituting the HA nonapeptide were selected as reaction units. After six rounds of synthesis, the capacity of the library was constructed to be 4 6 The peptide library, the ...
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