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Production method for surface plasma resonance imaging gold film spot micro-array

A technology of surface plasmon and resonance imaging, which is applied in the measurement of phase influence characteristics and scattering characteristics, and can solve problems such as non-specific adsorption and large SPR background

Active Publication Date: 2008-12-03
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of large SPR background and non-specific adsorption in the SPR imaging gold film microarray in the prior art, the present invention adopts the method of transparent film high-precision printing to prepare a mask, and then uses photolithography to prepare gold nanoparticles. Microarray, finally using electroless gold plating technology to prepare SPR imaging gold film dot microarray

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: An 8×8 SPR imaging gold film dot microarray with a gold dot diameter of 1000 μm was prepared.

[0013] (1) Preparation of glass flakes modified with colloidal gold nanoparticles

[0014] All glassware was cleaned with freshly prepared aqua regia washing solution and then rinsed with distilled water before use. The glass slides that are used to prepare SPR imaging gold film dot microarrays will also be soaked overnight with chromic acid lotion before use, and rinsed with secondary water; then put into newly prepared Piranha solution (3 volumes of sulfuric acid and 1 volume concentration of 30 % hydrogen peroxide mixture) at 70 degrees Celsius for 20 minutes to remove impurities on the surface.

[0015] After cleaning the glass piece, rinse it with deionized water and methanol solution; then immerse it in anhydrous methanol solution with a volume concentration of 10% 3-aminopropyltrimethoxysilane for surface silanization modification, take out the glass piece ...

Embodiment 2

[0021] Example 2: Preparation of an 8×8 SPR imaging gold film dot microarray with a gold dot diameter of 600 μm

[0022] (1) Preparation of glass flakes modified with colloidal gold nanoparticles

[0023] The preparation method of the glass sheet modified with colloidal gold nanoparticles is the same as that in Example 1.

[0024] (2) Preparation of glass slides decorated with gold nanoparticle dot microarrays by photolithography

[0025] The photolithography process is basically the same as that of Embodiment 1, the only difference is that the spot diameter of the patterned optical mask used in the exposure is different, and the spot diameter of the mask used in this example is 600 μm.

[0026] (3) Preparation of SPR imaging gold film dot microarray by electroless gold plating is the same as that in Example 1.

Embodiment 3

[0027] Example 3: Preparation of an 8×8 SPR imaging gold film dot microarray with a gold dot diameter of 300 μm

[0028] (1) Preparation of glass flakes modified with colloidal gold nanoparticles

[0029] The preparation method of the glass sheet modified with colloidal gold nanoparticles is the same as that in Example 1.

[0030] (2) Preparation of glass slides decorated with gold nanoparticle dot microarrays by photolithography

[0031] The photolithography process is basically the same as in Example 1, the only difference being that the patterned optical mask spot diameters used during exposure are different, and the mask spot diameters used in this example are 300 μm

[0032] (3) Preparation of SPR imaging gold film dot microarray by electroless gold plating is the same as that in Example 1.

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Abstract

The invention aims to provide a method for preparing surface plasma resonant imaging gold film micro-array. The method comprises the following steps: firstly self-assembling a gold size nanometer particle monolayer with the diameter of 2.5 nanometer on a glass plate modified by 3-Aminopropyltrimethoxysilane, taking the monolayer as a catalytic template; then taking a transparent film high-precision printed lattice array as a mask to carry out photo-etching to the glass plate modified by the gold nanometer particles, thereby preparing the micro-array of the gold nanometer particles; and controlling the gradual increase of the gold nanometer particle micro-array within the nanometer scale range by adopting the electroless plating technique, thereby constructing the required gold film micro-array. The size and the interval of each gold point in the micro-array can be conveniently controlled by the mask, and the thickness of the gold point is controlled by adjusting the electroless plating time.

Description

technical field [0001] The invention belongs to a preparation method of a surface plasma resonance imaging gold film dot microarray. Background technique [0002] Surface plasmon resonance (SPR) imaging technology is a rapid high-throughput analysis method combining SPR method with charge-coupled device (CCD) spectroscopy (Steiner G., Anal. Bioanal. Chem. 2004, 379, 328-331). This technique is simple to operate and can measure thousands of samples simultaneously. Therefore, SPR imaging technology has received attention at home and abroad, and has been widely used to study biomolecular interactions. The premise of applying SPR imaging technology to analyze biomolecular interactions is to prepare a qualified sample point array. So far, there are mainly three methods of lattice construction used in the reported SPR image analysis. The first is the combination of multi-step chemical modification of self-assembled monolayers and photosensitive chemical group etching under ultr...

Claims

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Application Information

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IPC IPC(8): G01N21/55G01N21/41
Inventor 董绍俊王建龙
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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