2 results about "Absolute calibration" patented technology

The application belongs to the field of gene sequencing, and discloses a microdroplet digital PCR absolute calibration method for mixed standard substances for high-throughput sequencer calibration and application thereof. The method comprises the following steps: providing N sets of primer probes for N targets in the standard substance; full background specificity verification: for the i th set of primer probes, it is necessary to prove that it can specifically detect the i th target, and prove that it has no specific detection signal in the mixed DNA sample composed of the remaining N-1 targets; only using all the primer probes verified in S2, performing parallel digital PCR detection on the standard substance sample; and calculating the absolute copy number concentration of each target according to the detection result. The method ensures the quantitative "purity" of each target in a complex mixed background, provides guarantee for high-confidence calibration results from the process design, and improves the throughput and efficiency of multi-target detection.

The application discloses an aerosol parameter inversion method based on a coherent Doppler laser radar, and comprises the following steps: emitting laser pulses to the atmosphere through the coherent Doppler laser radar, and receiving echo signals reflected back; correcting the echo signal carrier-to-noise ratio of the coherent Doppler laser radar in terms of distance, focusing function and system constant to obtain an attenuation backscattering coefficient; and inverting the attenuation backscattering coefficient to obtain the extinction coefficient and the backscattering coefficient of the aerosol. The application realizes absolute calibration of the echo signal intensity of the atmospheric aerosol, and inversion of the backscattering coefficient and the extinction coefficient of the aerosol.