Tumor microvascular imaging instrument and tumor microvascular imaging method

Abstract

The invention discloses a tumor microvascular imaging instrument and a tumor microvascular imaging method. The tumor microvascular imaging instrument comprises an infrared confocal imaging part and a control part, wherein the infrared confocal imaging part comprises a near-infrared laser, a dichroscope, a two-dimensional scanning galvanometer, a scanning lens system, an imaging objective, a near-infrared fluorescence filter, a convergent lens, a pinhole and a detector; the scanning lens system is composed of a field lens and a cylindrical lens; the control part comprises a two-dimensional scanning control module which is used for controlling the two-dimensional scanning galvanometer, a signal amplification and acquisition module and a data processing and image displaying module; near-infrared quantum dots that a fluorescence excitation spectrum ranges from 1000nm to 1350nm and a fluorescence emission spectrum ranges from 1350nm to 1500nm are injected to blood vessels of a to-be-observed living body; and then a tumor part of the living body is observed under the tumor microvascular imaging instrument. According to the tumor microvascular imaging instrument and the tumor microvascular imaging method provided by the invention, complete three-dimensional imaging of tumor microvessels of the living body can be implemented with a high resolution.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a tumor microvessel imager and a tumor microvessel imaging method, in particular to an imaging instrument with a working wavelength range of 1300-1500nm and a tumor microvessel imaging method. Background technique [0002] The formation of tumor microvessels has a very important impact on the growth and invasion and metastasis of tumors, especially on the early occurrence of tumors. Studies have shown that the rapid growth of tumors, especially malignant tumors, depends on angiogenesis. Some tumors are rich in microvessels, immature in shape, complex in network structure, and have obvious heterogeneity in composition, shape and spatial distribution, that is, phenotypic heterogeneity of tumor microvessel architecture. This heterogeneity may be derived from the imbalance of various angiogenesis-promoting and inhibitory factors in the regulatory mechanism of tumor angiogenesis. T...

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Problems solved by technology

At present, the clinical imaging methods for blood vessels mainly include ultrasound, CT angiography and nuclear magnetic angiography. However, it is still very difficult for these methods to distinguish capillary channels of tens of microns.

[0005] At present, optical imaging methods that are gradually beginning to be used in clinical applications have micron or even sub-micron resolution, and can easily distinguish microvessels, but the serious scattering of biological tissues limits the imaging depth of optical imaging methods. Coherent tomography and multiphoton imaging, the currently reported imaging depth is only about 1 mm

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