46 results about "Confocal imaging" patented technology

The invention relates to a structured light illumination confocal super-resolution measurement system and method, and relates to the technical field of optical measurement. The system comprises a digital micromirror device (DMD), a sample platform, an electric displacement platform, a microscopic imaging assembly, a complementary metal oxide semiconductor (CMOS) imaging assembly and computer equipment, and the DMD is used for adjusting the point light source array and the structured light field. According to the DMD-based confocal super-resolution measurement method, a structured light field and a signal-noise separation technology are combined, and high-precision and high-resolution three-dimensional surface reconstruction can be realized. By using the flexibility of the DMD and the advantages of the super-resolution technology, the spatial resolution and speed of confocal imaging are effectively improved, so that the measurement process is more accurate. In addition, low-rank decomposition and self-supervised deep learning methods are fused, the image reconstruction process is optimized, noise interference is suppressed, and the overall performance of the system is further improved.

The invention discloses a nanoparticle scattered light confocal imaging device and method. The device comprises a laser unit, a first pinhole, a beam splitter turntable, a scanning focusing unit, an electric objective table, a first detection imaging unit, a second pinhole, a light splitting unit and a second detection imaging unit. The method does not need to mark nano particles, and can synchronously collect unmarked nano particles and fluorescence-labeled biomolecules; in-situ imaging can be realized, and the distribution position and content of nano-particles in living cells can be directly observed; the imaging resolution ratio is high, single nanoparticle imaging can be carried out, and fluorescence imaging can also be carried out at the biomolecule level.

The invention relates to a refraction-super hybrid long-focus day and night confocal lens and an image acquisition device, and belongs to the field of optical lenses. The long-focus day and night confocal lens has focus consistency in a visible light band and an infrared band; comprising a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens and an eighth lens which are sequentially arranged from an object plane to an image plane along an optical axis; at least one of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens and the eighth lens is a metasurface lens, and the other lenses are spherical lenses. By adopting the technical scheme of folding and super mixing, large-target-surface day and night common-focal-length imaging is realized, the use of glued lenses is reduced, the complexity of the system is reduced, the assembly difficulty is reduced, and the risk that a structural member generates stray light is reduced.

The invention relates to a broadband short-wave infrared athermalization optical system. The optical system is a transmission type optical system. The structure of the optical system is a coaxial structure; the optical system is provided with a first positive lens, a first negative lens, a second positive lens, a second negative lens and a third negative lens in sequence in the light incidence direction. Light rays are converged through the lenses and are incident on the focal plane of the detector. According to the optical system provided by the invention, only five common infrared optical material lenses are used and matched with an aluminum alloy lens cone material, confocal imaging in a broadband range of 1.0 mu m-2. 5 mu m can be realized, the relative aperture is not greater than 1 / 0.4, athermalization of the optical system is realized, and the optical system is easy to manufacture, high in imaging quality and good in stability.

This invention discloses an illumination intensity modulation method for disk scanning confocal imaging, comprising the following steps: S1: Before formal imaging, a pre-imaging is performed using weak, uniform illumination; S2: The fluorescence intensity of different regions in the pre-imaging is evaluated and analyzed, and the evaluation results are obtained; S3: Based on the evaluation results, the parameter settings of the intensity modulation mechanism are adjusted to achieve different illumination in different regions of the sample; S4: The scanning points traverse the imaging area to obtain a complete image on the camera. This invention can dynamically adjust the illumination intensity of local regions in the scanning imaging as needed, and can be used to reduce photobleaching in local regions, increase the dynamic range of imaging, and enhance the signal-to-noise ratio of local regions.

The application discloses a coaxial spectrum confocal imaging system, comprising a light source module, a dispersion lens module, a spectrum imaging side slit device and a spectrometer; the light source module provides a wide-spectrum light beam and makes the light beam enter the dispersion lens module through a light source side slit device; the dispersion lens module makes the incident light sequentially pass through a first dispersion lens module, a light splitting device and a second dispersion lens module, and then is shot to a surface of a measured object, and also makes the light reflected by the surface of the measured object sequentially pass through the second dispersion lens module, is reflected by the light splitting device and passes through a third lens module, and then is converged to the spectrum imaging side slit device; the spectrometer images the light of different wavelengths converged to the spectrum imaging side slit device. The application overcomes the shielding problem of the prior art when measuring a relatively steep pit or hole by the mode that the incident light path and the reflected light path share a second dispersion lens module coaxially, and realizes the measurement of a deeper pit on the surface.

The utility model discloses a multi-cavity endoscope capable of simultaneously performing biopsy and confocal imaging, which comprises an endoscope main body, a fixing plate is arranged on the outer side of the endoscope main body, an anti-closing component is arranged on the fixing plate, a positioning component is arranged in the fixing plate, and the endoscope main body is provided with a cavity. The anti-closing part comprises cavities formed in the two sides of the interior of the fixing plate respectively, a rotating shaft connected with the cavities, a driving bevel gear fixedly connected with the outer side of the rotating shaft and driven bevel gears connected with the two sides of the driving bevel gear respectively. The problems that when an existing endoscope is used, although a human body can be examined, during examination, a patient needs to open the mouth, then the endoscope is put into the body of the patient, due to the fact that the endoscope has a certain size, the patient feels uncomfortable, the mouth is prone to being closed, movement of the endoscope is affected, the examination efficiency is affected, and the examination efficiency is affected are solved. And therefore, the practicability is reduced.

An apparatus for optically analyzing a sample may include an imaging subsystem that images the sample, one or more analyzing subsystems that analyze the sample including a confocal imaging subsystem, a temperature control subsystem that controls a temperature of the atmosphere within the apparatus, a gas control subsystem that controls a composition of the atmosphere within the apparatus, and a control module that controls the various subsystems of the apparatus.

The subject invention pertains to compositions of a probe comprising two pH-reversible forms, including the boron dipyrromethene (BODIPY) fluorescent form and the non-fluorescent leuco-BODIPY form, for use in confocal imaging methods of intracellular vesicles as well as extracellular vesicles that are present in conditioned culture medium and / or secreted by living cells, without inducing severe cytotoxicity.

The invention relates to a skin in-vivo confocal imaging image brightness adaptive adjusting device and method, and the method comprises the steps: employing a scanning imaging device to emit illumination light to a skin sample, and carrying out the point-by-point scanning imaging of the skin sample through a micro-electromechanical system in a point-by-point scanning mode; and the brightness of the illumination light is adaptively adjusted in an iterative mode through the brightness adjusting device. In the iteration process, the average signal intensity of the electric signals of the obtained imaging result in the sampling period and the number of the pixels with the signal intensity higher than the reference value are substituted into an empirical formula, an adjusting parameter value for adjusting the brightness of the illumination light in the next step is obtained, and then the brightness adjustment in the next step is carried out. Therefore, according to the self-adaptive adjusting device disclosed by the invention, the light emitting power of the light source does not need to be frequently and manually adjusted or the signal dynamic range in a software system does not need to be manually changed, special image processing is not needed, and the sample detection efficiency and the imaging effect can be improved.

The utility model discloses a device capable of simultaneously carrying out tissue cell collection and confocal imaging, which comprises a workbench and a puncture needle, one side of the workbench is provided with a collection detection mechanism, the collection detection mechanism comprises a transverse plate, one side of the transverse plate is fixedly connected with a hollow block, and the hollow block is fixedly connected with the puncture needle. An electric telescopic rod is fixedly connected to the front face of the hollow block, a connecting frame is fixedly connected to one side of the electric telescopic rod, and a sealing plate is fixedly connected to one side of the connecting frame. By arranging a collecting and detecting mechanism, starting an electric telescopic rod, driving a connecting frame to move by the electric telescopic rod, and driving a sealing plate to move by the connecting frame, negative pressure suction work is performed, cells are sucked into an inner cavity of a hollow block, and after part of the cells are sucked, the electric telescopic rod can be controlled to contract, so that the cells are pushed into an inner cavity of a fixed pipe through the sealing plate; cells are discharged to the top of the bearing plate through the fixing pipe, meanwhile, the bearing plate is moved to a detection position, and detection work is conducted through the detection probe.

The utility model provides a multi-mode fluorescence microscope. The multi-mode fluorescence microscope comprises a microscopic assembly, a near-infrared two-region excitation light path, a visible light confocal excitation light path, a wide-field imaging light path, a near-infrared two-region confocal imaging assembly, a multi-photon intensity / life harmonic wave imaging assembly and a visible light confocal and wide-field imaging assembly, the microscopic assembly comprises an objective lens, an optical filter switching assembly, a sleeve lens and a transflective device which are sequentially arranged from the object side to the image side, and the transflective device can be switched to a dichroscope or a reflector; the near-infrared second-area excitation light path, the visible light confocal excitation light path and the wide-field imaging light path are connected with the sleeve lens; the near-infrared two-region confocal imaging assembly, the multi-photon intensity / lifetime and harmonic imaging assembly and the visible light confocal and wide-field imaging assembly are all connected to the sleeve lens. According to the scheme, confocal and wide-field imaging of visible light, confocal and wide-field imaging of a near-infrared second region, and multi-photon intensity / lifetime and harmonic imaging based on near-infrared excitation are realized.

The utility model discloses a confocal perforated plate for tube formation experiment, which comprises a base plate structure, accommodating hole structures and bulge structures, the accommodating hole structures are equidistantly arranged on the upper surface of the base plate structure, and the bulge structures are arranged at the bottoms of the inner walls of the accommodating hole structures. According to the confocal perforated plate for the tube forming experiment, self-leveling of matrigel is achieved through combination of micron-sized annular protrusion arrays designed at the bottoms of the holes and super-hydrophilic surface modification, the standard deviation of the thickness of a gel layer is controlled within + / -3 microns, and the height difference of the liquid level is reduced to lt through side wall gradient hydrophobic treatment (top hydrophobic / bottom hydrophilic); 0.1 mm, and halo artifacts in confocal imaging are eliminated.

This invention relates to the fields of biomedical diagnostic reagent manufacturing and reproductive medicine technology, and particularly to a detection system and method for assessing sperm motility in patients with asthenospermia. This invention establishes a complete, accurate, and efficient molecular-level sperm quality evaluation system by integrating specific fluorescent labeling, high-resolution confocal imaging, and automated computer analysis software. This system not only overcomes the limitations of existing technologies but also further expands its functional scope by integrating an optical microscopy analysis module. It possesses good clinical applicability and scalability, providing a powerful tool for the etiological diagnosis, efficacy evaluation, and scientific research of male infertility, and has significant clinical application value and market prospects.

The invention discloses a method for observing the shape and number of synapses in a dentate gyrus region based on image analysis, and relates to the technical field of neurobiology. The method comprises the following steps: S1, tissue treatment: after an experimental animal is anesthetized, pouring a buffer solution and a stationary solution into the heart in stages, separating hippocampus tissue, trimming and fixing, pre-cooling, slicing and preserving at low temperature; s2, dyeing and marking: performing targeted marking on the dentate gyrus area of the sea horse by adopting DiI particles through a fine tungsten steel through needle, and preserving at low temperature after incubation; s3, fixing and mounting: fixing for the second time, and mounting with an anti-fluorescence quenching agent after DAPI nuclear dyeing; s4, confocal imaging is carried out, exclusive parameter layer scanning is set, and qualified dendritic spine images are screened; and image quantitative analysis can be further carried out, and accurate quantification is realized according to classification standards. The method is standard in operation, greatly improves sample quality and experimental result credibility, optimizes experimental efficiency and controllability and reduces technical threshold by means of targeted marking and standardized processes, and is suitable for relevant basis and application research of the hippocampal synapses.

The invention discloses a miniaturized confocal scanning probe. The miniaturized confocal scanning probe comprises a relay lens and an objective lens which are sequentially arranged along an optical axis, the relay lens is composed of at least one group of structure groups capable of being connected in series, the magnification factor is 1x, a structure combining a single lens and a doublet lens is adopted, the absolute value of the focal length of each lens does not exceed 1.5 times of the focal length of the single group of structure groups, the objective lens comprises a plurality of positive focal power lenses arranged along an optical axis, and the focal length distribution is characterized in that the two ends are short and the middle is long; an image point displacement group and a compensation group are arranged in the objective lens and can perform relative axial displacement along an optical axis, and tomography of different depths is realized under the condition that the relative position of the objective lens and a sample is not changed. The whole caliber of the probe can reach 7 mm, the imaging depth exceeds 300 micrometers, the length of the probe can be flexibly adjusted by increasing or decreasing the number of the relay lens sets so as to adapt to different application scenes such as the gynecology department and the oral cavity, and the technical blank in the field of hard scene high-resolution confocal imaging is filled up.

A kind of compression confocal imaging device and method based on digital micro-mirror device, the device includes confocal microscopic imaging module and single-pixel imaging compression detection module, the confocal microscopic imaging module and the single-pixel imaging compression detection module have the digital micro-mirror device and the sample detection light path between the digital micro-mirror device and sample, wherein, when confocal image acquisition is carried out to sample, the digital micro-mirror device becomes the confocal pinhole of the confocal microscopic imaging module, when single-pixel sampling is carried out to sample, the digital micro-mirror device becomes the confocal pinhole and single-pixel sampling light coding element of the single-pixel imaging compression detection module.Using the device and method of the application, confocal microscopic imaging can be realized under the condition of high speed low light toxicity sampling, the activity of biological sample can be fully guaranteed, and high spatiotemporal resolution imaging effect is provided.

The application relates to the technical field of industrial vision, in particular to a spectral confocal imaging lens, device, system and detection method, which can solve the problem of low depth direction detection sensitivity of a spectral confocal imaging device in three-dimensional detection application in a coaxial illumination mode to a certain extent. The focusing module and the dispersion module are arranged on the same side of the front shared module, the front shared module comprises a first light path submodule and a second light path submodule, the center positions of the first light path submodule and the dispersion module are on a first straight line; the center positions of the second light path submodule and the focusing module are on a second straight line; the first light path submodule receives a first light beam passing through the dispersion module, and converges the first light beam into a second light beam to irradiate on a to-be-detected object; the second light path submodule converts a third light beam into a fourth light beam, and transmits the fourth light beam to the focusing module; three-dimensional imaging detection is realized, and the detection sensitivity is improved.

The application belongs to the technical field of optical imaging, and particularly relates to a large-angle infrared confocal optical system and an intelligent monitoring lens module applied in the same. The system is mainly composed of five lenses. The first lens has a convex surface or a plane on the object side and a concave surface on the image side, and has a negative optical power; the second lens has a concave surface on the object side and a convex surface on the image side, and has a positive optical power; the third lens has a convex surface on the object side and a convex surface on the image side, and has a positive optical power; the fourth lens has a concave surface on the object side and a concave surface on the image side, and has a negative optical power; and the fifth lens has a convex surface on the object side and a convex surface on the image side, and has a positive optical power. The first lens, the second lens, the fourth lens and the fifth lens are high-transmittance plastic aspheric lenses. The system has a simple and compact structure. By precisely distributing the optical powers of the lenses and adopting the combination of high-transmittance plastic aspheric lenses and glass spherical lenses, the miniaturization and light weight of the lens are effectively realized, and a large field of view and excellent infrared confocal imaging quality are obtained.

An apparatus for optically analyzing a sample may include an imaging subsystem that images the sample, one or more analyzing subsystems that analyze the sample including a confocal imaging subsystem, a temperature control subsystem that controls a temperature of the atmosphere within the apparatus, a gas control subsystem that controls a composition of the atmosphere within the apparatus, and a control module that controls the various subsystems of the apparatus.

The present application relates to the field of microscopic optics, and especially to a point scanning confocal adjustment system and method, a light source emits light as a parallel light beam, the divergence angle is less than 1 mrad, the light beam reaches a galvanometer through a beam splitter, reaches a target object through a scanning lens group and an objective lens, returns after being reflected by the target object, reaches an adjustable mirror through a rotatable beam splitter, reaches a confocal aperture after being reflected and passing through a focusing lens; the confocal aperture is located at the back focal point of the lens, and confocal imaging is realized. Through a rotatable beam splitter, the present application realizes simultaneous observation of the imaging conditions of the target object and the confocal aperture position by using one camera. The present application solves the technical problem that, in the adjustment process, because the confocal aperture is small, in order to clearly see the confocal aperture, a camera with a high magnification is needed, the observable image surface range of the camera is very small, and if the initial position of the confocal aperture is not in the field of view of the camera, the confocal aperture position will be difficult to find.

An integrated multiplex measurement system for measuring semiconductor objects includes a main body, a fringe interferometer, a white light interferometer probe, and a color conjugate focal measurement probe. The fringe interferometer, white light interferometer probe, and color conjugate focal measurement probe are housed within the main body. The fringe interferometer can be used to measure the warp, unevenness, color, brightness distribution, shape, and high-nitrogen region distribution of the semiconductor object. The white light interferometer probe and color conjugate focal measurement probe are located at one point. The white light interferometer probe can measure the surface roughness and surface depth of the semiconductor object, while the color conjugate focal measurement probe can measure the surface roughness and thickness of the semiconductor object. It can accurately measure the surface roughness of the semiconductor object using confocal imaging dispersion analysis technology, and can also perform surface profile measurements, including fine curvature and structural features. This invention integrates multiple measuring devices into one machine, which can be used separately or together, thus facilitating the measurement of semiconductor objects.

The application discloses a glass aspheric wide-spectrum imaging lens to solve the imaging requirement of a wide waveband. The lens comprises four glass aspheric lenses and a protective glass coaxially arranged in sequence from an object side to an image side, wherein the first lens is a positive lens; the second lens is a negative lens; the third lens is a positive lens; and the fourth lens is a negative lens. The total length of the lens is 5.98 mm, the F# is 3.11, and the lens satisfies the requirement of the axial aberration being less than 50 microns in the confocal imaging in the waveband range of 340 nm-1550 nm. The lens system effectively corrects various aberrations in the waveband range of 340 nm-1550 nm by using the mode of matching the high-Abbe-number positive lens with the low-Abbe-number negative lens.

This invention discloses a method for detecting nucleic acid mutations with spatial single-cell resolution and its application. The method includes the following steps: (1) pre-staining, fixing, and digesting the tissue sample or cells to be tested; (2) adding isBDA reaction solution for in situ PCR amplification, discarding the reaction solution after the reaction, fixing again, and performing confocal imaging. This invention's detection method is an innovative in situ cell imaging detection method that can achieve localization and visualization at the single-cell level. This invention's detection method selectively inhibits the amplification of wild-type templates, thereby efficiently enriching the target mutation sequence. It can identify cell types while detecting mutation-positive cells, exhibiting high sensitivity and low cost. Furthermore, through specific reaction conditions, it maintains high inhibition efficiency in complex in situ environments, demonstrating good spatial single-cell resolution.

The present invention belongs to the technical field of single-mode fiber coupling and laser confocal imaging, and particularly relates to a single-mode fiber coupling apparatus and method in a laser confocal optical path. The apparatus comprises a coupling assembly and a bracket set. The coupling assembly comprises a laser, wherein an output end of the laser is connected to one end of a single-mode fiber I, and the other end of the single-mode fiber I is connected to an input end of a collimator; an output end of the collimator refracts light to a coupler through a dichroic mirror for coupling, and the coupler is mounted at a refraction end of the dichroic mirror; a CCD camera is mounted at a transmission end of the dichroic mirror for monitoring; and an output end of the coupler is connected to one end of a single-mode fiber II, and the other end of the single-mode fiber II is connected to an optical power meter. The bracket set comprises a collimator bracket, a dichroic mirror bracket and a coupler bracket, which respectively clamp the collimator, the dichroic mirror and the coupler. The present invention utilizes a real-time imaging function of a CCD camera to realize the accurate alignment of laser beams, thereby improving the coupling efficiency of single-mode fibers.

The invention provides a high-pixel confocal imaging optical system and a camera module using the same, the high-pixel confocal imaging optical system is mainly composed of seven lenses, the first lens has negative focal power, the object side surface of the first lens is a convex surface, the image side surface of the first lens is a concave surface, the second lens has focal power, the object side surface of the second lens is a concave surface, the image side surface of the second lens is a convex surface, the third lens has positive focal power, and the image side surface of the third lens is a convex surface. The fifth lens has negative focal power, the object side surface of the fifth lens is a concave surface, the image side surface of the fifth lens is a concave surface, the sixth lens has positive focal power, the object side surface of the sixth lens is a convex surface, the image side surface of the sixth lens is a convex surface, the seventh lens has focal power, the object side surface of the seventh lens is a convex surface, and the object side surface of the seventh lens is a convex surface. Through the reasonable matching of the lens shape and the focal power, the design requirements of confocal, high resolution and miniaturization of the optical imaging system are effectively met, the advantages of high pixel, confocal, miniaturization and low cost are achieved, and good and stable performance can be continuously kept in a severe environment.

The invention provides a method for marking a microfilament skeleton in a living cell by using ligand-modified magnetic nanoparticles and application of the microfilament skeleton. The method comprises the following steps: preparing ligand modified magnetic nanoparticles; culturing living cells to be marked to obtain adherent cells; incubating the adherent cells and the ligand modified magnetic nanoparticles to obtain marked cells; and observing the marked intracellular microfilament skeleton by using a laser confocal imaging system. The ligand-modified magnetic nanoparticles prepared by the method have good biocompatibility, magnetic responsiveness and microfilament targeted binding characteristics, microfilament skeletons in living cells are labeled by the ligand-modified magnetic nanoparticles, and then distribution of the microfilament skeletons in the living cells can be clearly observed by using a laser confocal imaging system. According to the method, the microfilament skeleton in the living cell can be marked and clearly imaged, the distribution of the microfilament skeleton in the living cell can be deeply observed, and the method has a good application prospect in the fields of biomedical treatment, cell function research and the like.

The invention provides an intelligent collaborative multispectral confocal electrochromic filtering module, belongs to the technical field of multispectral confocal detection and electrochromic filtering, and is used for solving the problems of poor intergenerational suitability, insufficient confocal precision and weak environmental adaptability of the filtering module in the related technology. The confocal lens group comprises core components such as a confocal adaptive electrochromic film and a multi-physics field sensing unit, the core components are coaxially assembled along the optical axis of the lens group to form an integrated collaborative architecture, and seamless compatibility of the one-time to three-time improved confocal lens group is realized through intergenerational identification, multi-physics field closed-loop calibration and filtering-lens collaborative adaptation; low power consumption and strong environment adaptability are achieved, and the accuracy and stability of full-band confocal imaging are effectively guaranteed.