72 results about "Confocal imaging" patented technology

The invention relates to a structured light illumination confocal super-resolution measurement system and method, and relates to the technical field of optical measurement. The system comprises a digital micromirror device (DMD), a sample platform, an electric displacement platform, a microscopic imaging assembly, a complementary metal oxide semiconductor (CMOS) imaging assembly and computer equipment, and the DMD is used for adjusting the point light source array and the structured light field. According to the DMD-based confocal super-resolution measurement method, a structured light field and a signal-noise separation technology are combined, and high-precision and high-resolution three-dimensional surface reconstruction can be realized. By using the flexibility of the DMD and the advantages of the super-resolution technology, the spatial resolution and speed of confocal imaging are effectively improved, so that the measurement process is more accurate. In addition, low-rank decomposition and self-supervised deep learning methods are fused, the image reconstruction process is optimized, noise interference is suppressed, and the overall performance of the system is further improved.

The invention discloses a nanoparticle scattered light confocal imaging device and method. The device comprises a laser unit, a first pinhole, a beam splitter turntable, a scanning focusing unit, an electric objective table, a first detection imaging unit, a second pinhole, a light splitting unit and a second detection imaging unit. The method does not need to mark nano particles, and can synchronously collect unmarked nano particles and fluorescence-labeled biomolecules; in-situ imaging can be realized, and the distribution position and content of nano-particles in living cells can be directly observed; the imaging resolution ratio is high, single nanoparticle imaging can be carried out, and fluorescence imaging can also be carried out at the biomolecule level.

The invention relates to a refraction-super hybrid long-focus day and night confocal lens and an image acquisition device, and belongs to the field of optical lenses. The long-focus day and night confocal lens has focus consistency in a visible light band and an infrared band; comprising a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens and an eighth lens which are sequentially arranged from an object plane to an image plane along an optical axis; at least one of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens and the eighth lens is a metasurface lens, and the other lenses are spherical lenses. By adopting the technical scheme of folding and super mixing, large-target-surface day and night common-focal-length imaging is realized, the use of glued lenses is reduced, the complexity of the system is reduced, the assembly difficulty is reduced, and the risk that a structural member generates stray light is reduced.

The invention discloses a fluorescent confocal microscopic endoscopic imaging lens, which comprises three groups of doublet lenses, specifically comprising a first lens group, a second lens group and a third lens group, the first lens group comprises a plano-concave lens and a first biconvex lens which are sequentially arranged from an object space side to an image space side, and the second lens group comprises a plano-concave lens and a second biconvex lens which are sequentially arranged from an object space side to an image space side; the detector is used for collecting fluorescence signals emitted by an object space and adjusting light angles; the second lens group comprises a first negative meniscus lens and a second biconvex lens, and is used for relaying transmission light and balancing aberration so as to gently transition a light angle; and the third lens group comprises a second negative meniscus lens and a positive meniscus lens and is used for focusing the light to the end face of the optical fiber bundle. The lens is compact in structure, reasonable in light path matching and suitable for human tissue close imaging scenes, has the advantages of being high in resolution, low in distortion, excellent in chromatic aberration control and the like, and is suitable for digestive tract early cancer diagnosis and confocal imaging system integration.

The invention relates to a broadband short-wave infrared athermalization optical system. The optical system is a transmission type optical system. The structure of the optical system is a coaxial structure; the optical system is provided with a first positive lens, a first negative lens, a second positive lens, a second negative lens and a third negative lens in sequence in the light incidence direction. Light rays are converged through the lenses and are incident on the focal plane of the detector. According to the optical system provided by the invention, only five common infrared optical material lenses are used and matched with an aluminum alloy lens cone material, confocal imaging in a broadband range of 1.0 mu m-2. 5 mu m can be realized, the relative aperture is not greater than 1 / 0.4, athermalization of the optical system is realized, and the optical system is easy to manufacture, high in imaging quality and good in stability.

The invention relates to a whole genome multi-recombinant protein modification labeling method based on transposition reaction and fluorescence in situ hybridization and application thereof, and belongs to the technical field of cell molecular markers. The invention relates to a marking method modified by a whole genome multi-recombinant protein. The marking method comprises the following steps: cleaning cells carrying one or more special sequences for coding oligonucleotides; and mixing the cleaned cells, an EC buffer solution and an aqueous solution of the oligonucleotide fluorescent probe, carrying out room temperature incubation, discarding the liquid, cleaning, and carrying out fluorescence imaging. According to the labeling method, fluorescence in-situ labeling can be carried out on different types of fine histone modifications in the same cell sample, and the labeling method is suitable for an ultrahigh-resolution microscopic imaging platform or a common confocal imaging system. The marker has extremely high specificity and sensitivity, and spatial distribution and co-localization information of various histone modifications in a cell nucleus can be obtained.

This invention discloses an illumination intensity modulation method for disk scanning confocal imaging, comprising the following steps: S1: Before formal imaging, a pre-imaging is performed using weak, uniform illumination; S2: The fluorescence intensity of different regions in the pre-imaging is evaluated and analyzed, and the evaluation results are obtained; S3: Based on the evaluation results, the parameter settings of the intensity modulation mechanism are adjusted to achieve different illumination in different regions of the sample; S4: The scanning points traverse the imaging area to obtain a complete image on the camera. This invention can dynamically adjust the illumination intensity of local regions in the scanning imaging as needed, and can be used to reduce photobleaching in local regions, increase the dynamic range of imaging, and enhance the signal-to-noise ratio of local regions.

The invention relates to the technical field of microscopy optics, in particular to a spot scanning confocal adjusting system and method, light emitted by a light source is parallel light beams, the divergence angle is smaller than 1mrad, the light beams reach a galvanometer through a spectroscope, reach a target object through a scanning mirror set and an objective lens, return after being reflected by the target object, pass through the spectroscope capable of rotating, and then pass through a scanning mirror set. After being reflected, the laser reaches the confocal hole through the focusing lens; the confocal hole is located at the rear focus of the lens, and confocal imaging is achieved. According to the invention, through the rotatable spectroscope, the imaging condition of the target object at the confocal hole position can be observed by one camera at the same time. The technical problem that the position of the confocal hole is difficult to find if the initial position of the confocal hole is not within the view field range of the camera due to the fact that the confocal hole is small, a camera with a high amplification factor needs to be selected for clearly seeing the confocal hole and the image plane range which can be observed by the camera is very small in the debugging process is solved.

The present invention discloses a high-precision spectral confocal microscopy imaging system, comprising a light modulation module, a spectrum splitting module, a scanning imaging module, an image detection device and a computer. The light beam emitted by the light modulation module passes through the scanning imaging module and is incident on a sample to excite a fluorescence signal. The fluorescence signal passes through the spectrum splitting module and is received by the image detection device. The image detection device converts the current signal into a digital signal and transmits it to the computer to complete imaging. The system is characterized in that the spectrum splitting module consists of a first converging lens, a first pinhole, a first linear long-wave pass filter, a second converging lens, a first linear short-wave pass filter and a third converging lens arranged in sequence. The system has the advantage of realizing high-precision spectral confocal imaging by combining lenses and linear filters on the basis of the traditional confocal microscopy imaging principle, solving the problem of spectral crosstalk in confocal imaging, and achieving a spectral resolution of less than 3.0 nm.

The application discloses a coaxial spectrum confocal imaging system, comprising a light source module, a dispersion lens module, a spectrum imaging side slit device and a spectrometer; the light source module provides a wide-spectrum light beam and makes the light beam enter the dispersion lens module through a light source side slit device; the dispersion lens module makes the incident light sequentially pass through a first dispersion lens module, a light splitting device and a second dispersion lens module, and then is shot to a surface of a measured object, and also makes the light reflected by the surface of the measured object sequentially pass through the second dispersion lens module, is reflected by the light splitting device and passes through a third lens module, and then is converged to the spectrum imaging side slit device; the spectrometer images the light of different wavelengths converged to the spectrum imaging side slit device. The application overcomes the shielding problem of the prior art when measuring a relatively steep pit or hole by the mode that the incident light path and the reflected light path share a second dispersion lens module coaxially, and realizes the measurement of a deeper pit on the surface.

The utility model discloses a multi-cavity endoscope capable of simultaneously performing biopsy and confocal imaging, which comprises an endoscope main body, a fixing plate is arranged on the outer side of the endoscope main body, an anti-closing component is arranged on the fixing plate, a positioning component is arranged in the fixing plate, and the endoscope main body is provided with a cavity. The anti-closing part comprises cavities formed in the two sides of the interior of the fixing plate respectively, a rotating shaft connected with the cavities, a driving bevel gear fixedly connected with the outer side of the rotating shaft and driven bevel gears connected with the two sides of the driving bevel gear respectively. The problems that when an existing endoscope is used, although a human body can be examined, during examination, a patient needs to open the mouth, then the endoscope is put into the body of the patient, due to the fact that the endoscope has a certain size, the patient feels uncomfortable, the mouth is prone to being closed, movement of the endoscope is affected, the examination efficiency is affected, and the examination efficiency is affected are solved. And therefore, the practicability is reduced.

A porphyrin compound for bioimaging and sonodynamic therapy, as well as its preparation method and application, has a chemical formula shown in Formula 2. The preparation method comprises the following steps: adding p-methylthiobenzaldehyde, a compound of Formula 1, and pyrrole to a reaction flask, evacuating the flask and introducing nitrogen gas, followed by adding anhydrous dichloromethane to obtain a reaction solution; bubbling nitrogen gas through the reaction solution, stirring at 25°C, adding boron trifluoride etherate, maintaining the temperature while continuing to stir, adding DDQ, continuing stirring, adding triethylamine, filtering, concentrating, and purifying to obtain the target product. This method features mild reaction conditions, a short reaction time, and simple operation. The resulting porphyrin compound exhibits excellent sonosensitivity and biocompatibility, can efficiently generate reactive oxygen species under ultrasound, and can produce excellent sonodynamic effects. The compound also exhibits fluorescence emission in the near-infrared region and can be excited by visible light, enabling confocal imaging to detect changes in cell morphology before and after, resulting in excellent bioimaging results. #imgabs0#

An apparatus for optically analyzing a sample may include an imaging subsystem that images the sample, one or more analyzing subsystems that analyze the sample including a confocal imaging subsystem, a temperature control subsystem that controls a temperature of the atmosphere within the apparatus, a gas control subsystem that controls a composition of the atmosphere within the apparatus, and a control module that controls the various subsystems of the apparatus.

The invention discloses a wide-spectrum optical system and a lens, and relates to the technical field of optical imaging. The optical system comprises twelve lenses which are sequentially arranged from the object space to the image space, the fifth lens and the sixth lens form a first balsaming lens, and the seventh lens and the eighth lens form a second balsaming lens; the ninth lens, the tenth lens and the eleventh lens form a third cemented lens. The second lens, the third lens, the sixth lens, the seventh lens, the ninth lens and the eleventh lens are lenses with positive focal power, the twelfth lens is a plane lens, and the others are lenses with negative focal power. According to the wide-spectrum optical system and the lens provided by the invention, through a special optical structure consisting of twelve lenses, dispersion aberration and geometric aberration in a range of 400-1700 nanometers can be corrected at the same time, so that high-precision confocal imaging is realized, and the performance of a spectrum imaging system is remarkably improved.

The subject invention pertains to compositions of a probe comprising two pH-reversible forms, including the boron dipyrromethene (BODIPY) fluorescent form and the non-fluorescent leuco-BODIPY form, for use in confocal imaging methods of intracellular vesicles as well as extracellular vesicles that are present in conditioned culture medium and / or secreted by living cells, without inducing severe cytotoxicity.

The application provides a single-objective multi-mode microscopic imaging system, and relates to the technical field of microscopic imaging.The system comprises a laser light source, an excitation light path module, a modulation scanning module, a detection light path module, an imaging camera and a controller.The excitation light path module comprises an expansion-constriction lens group, a shaping unit, a first slit and a first relay lens group.The modulation scanning module comprises a dynamic light splitting unit in a non-fixed position, a first scanning galvanometer, a scanning lens, a barrel lens and an objective lens.The detection light path module comprises a first lens, a second relay lens group, a second slit in a non-fixed state, an electronically adjustable lens, a third relay lens group and a second scanning galvanometer.The modulation scanning module is used for transmitting a laser beam to the back focal plane of the objective lens and relaying sample fluorescence collected by the objective lens to the detection light path module.The controller is used for switching between oblique light sheet imaging mode and confocal imaging mode according to the position of the dynamic light splitting unit and the state of the second slit.

The invention relates to a skin in-vivo confocal imaging image brightness adaptive adjusting device and method, and the method comprises the steps: employing a scanning imaging device to emit illumination light to a skin sample, and carrying out the point-by-point scanning imaging of the skin sample through a micro-electromechanical system in a point-by-point scanning mode; and the brightness of the illumination light is adaptively adjusted in an iterative mode through the brightness adjusting device. In the iteration process, the average signal intensity of the electric signals of the obtained imaging result in the sampling period and the number of the pixels with the signal intensity higher than the reference value are substituted into an empirical formula, an adjusting parameter value for adjusting the brightness of the illumination light in the next step is obtained, and then the brightness adjustment in the next step is carried out. Therefore, according to the self-adaptive adjusting device disclosed by the invention, the light emitting power of the light source does not need to be frequently and manually adjusted or the signal dynamic range in a software system does not need to be manually changed, special image processing is not needed, and the sample detection efficiency and the imaging effect can be improved.

The utility model discloses a device capable of simultaneously carrying out tissue cell collection and confocal imaging, which comprises a workbench and a puncture needle, one side of the workbench is provided with a collection detection mechanism, the collection detection mechanism comprises a transverse plate, one side of the transverse plate is fixedly connected with a hollow block, and the hollow block is fixedly connected with the puncture needle. An electric telescopic rod is fixedly connected to the front face of the hollow block, a connecting frame is fixedly connected to one side of the electric telescopic rod, and a sealing plate is fixedly connected to one side of the connecting frame. By arranging a collecting and detecting mechanism, starting an electric telescopic rod, driving a connecting frame to move by the electric telescopic rod, and driving a sealing plate to move by the connecting frame, negative pressure suction work is performed, cells are sucked into an inner cavity of a hollow block, and after part of the cells are sucked, the electric telescopic rod can be controlled to contract, so that the cells are pushed into an inner cavity of a fixed pipe through the sealing plate; cells are discharged to the top of the bearing plate through the fixing pipe, meanwhile, the bearing plate is moved to a detection position, and detection work is conducted through the detection probe.

A multi-depth confocal imaging system includes at least one light source configured to provide excitation beams and an objective lens. The excitation beams are focused into a sample at a first plurality of focus depths along an excitation direction through the objective lens. An image sensor receives emissions from the sample via the objective lens, wherein the emissions define foci relative to the image sensor at a second plurality of focus depths.

The invention discloses a pressurized constant-temperature incubator for three-dimensional culture of stem cells and a cell culture method, and relates to the technical field of three-dimensional culture of stem cells. The environment control structure comprises a closed-loop gas circulation flow channel arranged in the incubator main body, and through the synergistic effect of a gas circulation driving mechanism and a mechanical stimulation assembly, the complex mechanical environment of in-vivo multi-physical field coupling is accurately simulated; meanwhile, osmotic pressure change and chemical stress caused by traditional liquid perfusion are perfectly avoided by utilizing closed-loop gas circulation. The piezoelectric ceramic actuator group supports high-flux and personalized research, a time-sharing working strategy realizes interference-free in-situ dynamic monitoring of mechanical stimulation and high-precision confocal imaging, and a transmission control assembly further realizes intelligent linkage of airflow guiding and electromagnetic buffering adjustment, so that the stimulation accuracy and purity are ensured, the overall high integration and automation are realized, and the mechanical stimulation and high-precision confocal imaging are realized. And reliable data support is provided for three-dimensional culture of stem cells.

The utility model provides a multi-mode fluorescence microscope. The multi-mode fluorescence microscope comprises a microscopic assembly, a near-infrared two-region excitation light path, a visible light confocal excitation light path, a wide-field imaging light path, a near-infrared two-region confocal imaging assembly, a multi-photon intensity / life harmonic wave imaging assembly and a visible light confocal and wide-field imaging assembly, the microscopic assembly comprises an objective lens, an optical filter switching assembly, a sleeve lens and a transflective device which are sequentially arranged from the object side to the image side, and the transflective device can be switched to a dichroscope or a reflector; the near-infrared second-area excitation light path, the visible light confocal excitation light path and the wide-field imaging light path are connected with the sleeve lens; the near-infrared two-region confocal imaging assembly, the multi-photon intensity / lifetime and harmonic imaging assembly and the visible light confocal and wide-field imaging assembly are all connected to the sleeve lens. According to the scheme, confocal and wide-field imaging of visible light, confocal and wide-field imaging of a near-infrared second region, and multi-photon intensity / lifetime and harmonic imaging based on near-infrared excitation are realized.

The utility model discloses a confocal perforated plate for tube formation experiment, which comprises a base plate structure, accommodating hole structures and bulge structures, the accommodating hole structures are equidistantly arranged on the upper surface of the base plate structure, and the bulge structures are arranged at the bottoms of the inner walls of the accommodating hole structures. According to the confocal perforated plate for the tube forming experiment, self-leveling of matrigel is achieved through combination of micron-sized annular protrusion arrays designed at the bottoms of the holes and super-hydrophilic surface modification, the standard deviation of the thickness of a gel layer is controlled within + / -3 microns, and the height difference of the liquid level is reduced to lt through side wall gradient hydrophobic treatment (top hydrophobic / bottom hydrophilic); 0.1 mm, and halo artifacts in confocal imaging are eliminated.

This invention relates to the fields of biomedical diagnostic reagent manufacturing and reproductive medicine technology, and particularly to a detection system and method for assessing sperm motility in patients with asthenospermia. This invention establishes a complete, accurate, and efficient molecular-level sperm quality evaluation system by integrating specific fluorescent labeling, high-resolution confocal imaging, and automated computer analysis software. This system not only overcomes the limitations of existing technologies but also further expands its functional scope by integrating an optical microscopy analysis module. It possesses good clinical applicability and scalability, providing a powerful tool for the etiological diagnosis, efficacy evaluation, and scientific research of male infertility, and has significant clinical application value and market prospects.

The invention discloses a method for observing the shape and number of synapses in a dentate gyrus region based on image analysis, and relates to the technical field of neurobiology. The method comprises the following steps: S1, tissue treatment: after an experimental animal is anesthetized, pouring a buffer solution and a stationary solution into the heart in stages, separating hippocampus tissue, trimming and fixing, pre-cooling, slicing and preserving at low temperature; s2, dyeing and marking: performing targeted marking on the dentate gyrus area of the sea horse by adopting DiI particles through a fine tungsten steel through needle, and preserving at low temperature after incubation; s3, fixing and mounting: fixing for the second time, and mounting with an anti-fluorescence quenching agent after DAPI nuclear dyeing; s4, confocal imaging is carried out, exclusive parameter layer scanning is set, and qualified dendritic spine images are screened; and image quantitative analysis can be further carried out, and accurate quantification is realized according to classification standards. The method is standard in operation, greatly improves sample quality and experimental result credibility, optimizes experimental efficiency and controllability and reduces technical threshold by means of targeted marking and standardized processes, and is suitable for relevant basis and application research of the hippocampal synapses.

The invention discloses a miniaturized confocal scanning probe. The miniaturized confocal scanning probe comprises a relay lens and an objective lens which are sequentially arranged along an optical axis, the relay lens is composed of at least one group of structure groups capable of being connected in series, the magnification factor is 1x, a structure combining a single lens and a doublet lens is adopted, the absolute value of the focal length of each lens does not exceed 1.5 times of the focal length of the single group of structure groups, the objective lens comprises a plurality of positive focal power lenses arranged along an optical axis, and the focal length distribution is characterized in that the two ends are short and the middle is long; an image point displacement group and a compensation group are arranged in the objective lens and can perform relative axial displacement along an optical axis, and tomography of different depths is realized under the condition that the relative position of the objective lens and a sample is not changed. The whole caliber of the probe can reach 7 mm, the imaging depth exceeds 300 micrometers, the length of the probe can be flexibly adjusted by increasing or decreasing the number of the relay lens sets so as to adapt to different application scenes such as the gynecology department and the oral cavity, and the technical blank in the field of hard scene high-resolution confocal imaging is filled up.

The invention belongs to the field of medicine screening and biological detection, and particularly relates to a fluorescent probe for screening an anti-atherosclerosis traditional Chinese medicine as well as a preparation method and application of the fluorescent probe. The invention aims to construct an anti-atherosclerosis traditional Chinese medicine screening platform based on a fluorescent probe technology to screen 70 traditional Chinese medicines. Firstly, a probe for identifying ONOO is designed and synthesized to serve as a tool for detecting the ROS content in the zebrafish body, then lipopolysaccharide is used for inducing and constructing a zebrafish atherosclerosis model, and the confocal imaging technology is used for dynamically monitoring the improvement conditions of different traditional Chinese medicine crude extracts on the atherosclerosis in the zebrafish body. The invention not only provides an innovative method for efficient screening of traditional Chinese medicine anti-atherosclerosis components, but also provides potential drug candidates for prevention and treatment of atherosclerosis.

A kind of compression confocal imaging device and method based on digital micro-mirror device, the device includes confocal microscopic imaging module and single-pixel imaging compression detection module, the confocal microscopic imaging module and the single-pixel imaging compression detection module have the digital micro-mirror device and the sample detection light path between the digital micro-mirror device and sample, wherein, when confocal image acquisition is carried out to sample, the digital micro-mirror device becomes the confocal pinhole of the confocal microscopic imaging module, when single-pixel sampling is carried out to sample, the digital micro-mirror device becomes the confocal pinhole and single-pixel sampling light coding element of the single-pixel imaging compression detection module.Using the device and method of the application, confocal microscopic imaging can be realized under the condition of high speed low light toxicity sampling, the activity of biological sample can be fully guaranteed, and high spatiotemporal resolution imaging effect is provided.

The present application belongs to the field of drug screening and biological detection, and particularly relates to a fluorescent probe for screening anti-atherosclerotic traditional Chinese medicines and a preparation method and application thereof. The present application aims to construct an anti-atherosclerotic traditional Chinese medicine screening platform based on fluorescent probe technology, and screen 70 kinds of traditional Chinese medicines. First, a probe for recognizing ONOO⁻ is designed and synthesized as a tool for detecting the ROS content in zebrafish, then a zebrafish atherosclerosis model is constructed by using lipopolysaccharide induction, and the improvement of different traditional Chinese medicine crude extracts on the atherosclerosis in zebrafish is dynamically monitored by using confocal imaging technology. The present application not only provides an innovative method for the efficient screening of anti-atherosclerotic components of traditional Chinese medicines, but also provides potential drug candidates for the prevention and treatment of atherosclerosis.

The application relates to the technical field of industrial vision, in particular to a spectral confocal imaging lens, device, system and detection method, which can solve the problem of low depth direction detection sensitivity of a spectral confocal imaging device in three-dimensional detection application in a coaxial illumination mode to a certain extent. The focusing module and the dispersion module are arranged on the same side of the front shared module, the front shared module comprises a first light path submodule and a second light path submodule, the center positions of the first light path submodule and the dispersion module are on a first straight line; the center positions of the second light path submodule and the focusing module are on a second straight line; the first light path submodule receives a first light beam passing through the dispersion module, and converges the first light beam into a second light beam to irradiate on a to-be-detected object; the second light path submodule converts a third light beam into a fourth light beam, and transmits the fourth light beam to the focusing module; three-dimensional imaging detection is realized, and the detection sensitivity is improved.