93 results about "Confocal imaging" patented technology

The present invention discloses a device and method for semiconductor grain double-sided simultaneous fully equal optical path confocal imaging detection based on polarization separation imaging method. The device includes a CMOS or CCD polarization camera, a telecentric imaging lens, a polarization cube beam splitter, a right-angle image rotation prism, a semiconductor grain, and a transparent glass stage for placing the semiconductor grain, which are sequentially arranged in the optical path direction. The advantages of the device and method of the present invention are as follows: It can achieve double-sided simultaneous fully equal optical path confocal polarization imaging detection of the object to be measured, that is, △ = 0, without the need to use a large-depth-of-field telecentric lens to compensate for the optical path difference of double-sided imaging; the double-sided simultaneous polarization imaging detection device has a simple and compact structure and is easy to assemble and debug.

The present invention belongs to the technical field related to precision measurement, and discloses an orthogonal line spectrum confocal scanning profile measurement sensor and an anti-vibration method. The sensor includes a light source module, and first and second direction linear dispersion confocal imaging modules. The light source module is used to generate two beams of orthogonal collimated white light. The two beams of orthogonal collimated white light enter the first and second direction linear dispersion confocal imaging modules respectively, thereby scanning the surface of the sample to be measured and imaging, thereby obtaining the surface profile of the sample to be measured at two orthogonal angles. The first direction linear dispersion confocal imaging module includes a first linear dispersion confocal structure, a second slit, and a first imaging unit. The second direction linear dispersion confocal imaging module includes a second linear dispersion confocal structure, a third slit, and a second imaging unit. The directions of the second and third slits are respectively the same as the directions of the two orthogonal slits in the first slit. Through the present invention, the problem of vibration affecting measurement accuracy during line scanning surface measurement is solved.

The invention relates to a structured light illumination confocal super-resolution measurement system and method, and relates to the technical field of optical measurement. The system comprises a digital micromirror device (DMD), a sample platform, an electric displacement platform, a microscopic imaging assembly, a complementary metal oxide semiconductor (CMOS) imaging assembly and computer equipment, and the DMD is used for adjusting the point light source array and the structured light field. According to the DMD-based confocal super-resolution measurement method, a structured light field and a signal-noise separation technology are combined, and high-precision and high-resolution three-dimensional surface reconstruction can be realized. By using the flexibility of the DMD and the advantages of the super-resolution technology, the spatial resolution and speed of confocal imaging are effectively improved, so that the measurement process is more accurate. In addition, low-rank decomposition and self-supervised deep learning methods are fused, the image reconstruction process is optimized, noise interference is suppressed, and the overall performance of the system is further improved.

The invention discloses a nanoparticle scattered light confocal imaging device and method. The device comprises a laser unit, a first pinhole, a beam splitter turntable, a scanning focusing unit, an electric objective table, a first detection imaging unit, a second pinhole, a light splitting unit and a second detection imaging unit. The method does not need to mark nano particles, and can synchronously collect unmarked nano particles and fluorescence-labeled biomolecules; in-situ imaging can be realized, and the distribution position and content of nano-particles in living cells can be directly observed; the imaging resolution ratio is high, single nanoparticle imaging can be carried out, and fluorescence imaging can also be carried out at the biomolecule level.

The invention provides a line scanning differential confocal Raman microscopic spectrum co-fusion imaging system, which belongs to the microscopic imaging technology, and comprises a light spot illumination module, a confocal imaging module, a Raman imaging module, a line scanning module and a control module, the confocal imaging module is used for enabling reflected light of a sample to be detected to equivalently enter the first camera, the second camera and the third camera so as to obtain pre-focus, focal plane and post-focus images of the sample to be detected, and the Raman imaging module is used for obtaining chemical Raman component spectrum information; the control module obtains a confocal image with surface height information of the to-be-detected sample according to the pre-focus image, the focal plane image and the post-focus image of the to-be-detected sample, and synchronously fuses the confocal image and the chemical Raman component spectrum information to obtain a four-dimensional image comprising the three-dimensional surface topography and the chemical Raman component spectrum of the to-be-detected sample. According to the invention, map co-fusion can be realized, and more visual multi-dimensional measurement information can be obtained.

The invention relates to a refraction-super hybrid long-focus day and night confocal lens and an image acquisition device, and belongs to the field of optical lenses. The long-focus day and night confocal lens has focus consistency in a visible light band and an infrared band; comprising a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens and an eighth lens which are sequentially arranged from an object plane to an image plane along an optical axis; at least one of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens and the eighth lens is a metasurface lens, and the other lenses are spherical lenses. By adopting the technical scheme of folding and super mixing, large-target-surface day and night common-focal-length imaging is realized, the use of glued lenses is reduced, the complexity of the system is reduced, the assembly difficulty is reduced, and the risk that a structural member generates stray light is reduced.

The invention discloses a fluorescent confocal microscopic endoscopic imaging lens, which comprises three groups of doublet lenses, specifically comprising a first lens group, a second lens group and a third lens group, the first lens group comprises a plano-concave lens and a first biconvex lens which are sequentially arranged from an object space side to an image space side, and the second lens group comprises a plano-concave lens and a second biconvex lens which are sequentially arranged from an object space side to an image space side; the detector is used for collecting fluorescence signals emitted by an object space and adjusting light angles; the second lens group comprises a first negative meniscus lens and a second biconvex lens, and is used for relaying transmission light and balancing aberration so as to gently transition a light angle; and the third lens group comprises a second negative meniscus lens and a positive meniscus lens and is used for focusing the light to the end face of the optical fiber bundle. The lens is compact in structure, reasonable in light path matching and suitable for human tissue close imaging scenes, has the advantages of being high in resolution, low in distortion, excellent in chromatic aberration control and the like, and is suitable for digestive tract early cancer diagnosis and confocal imaging system integration.

The invention relates to a broadband short-wave infrared athermalization optical system. The optical system is a transmission type optical system. The structure of the optical system is a coaxial structure; the optical system is provided with a first positive lens, a first negative lens, a second positive lens, a second negative lens and a third negative lens in sequence in the light incidence direction. Light rays are converged through the lenses and are incident on the focal plane of the detector. According to the optical system provided by the invention, only five common infrared optical material lenses are used and matched with an aluminum alloy lens cone material, confocal imaging in a broadband range of 1.0 mu m-2. 5 mu m can be realized, the relative aperture is not greater than 1 / 0.4, athermalization of the optical system is realized, and the optical system is easy to manufacture, high in imaging quality and good in stability.

The invention discloses a fixed-focus high-definition long-focus infrared confocal imaging optical system, and relates to the technical field of optical imaging equipment. The optical system sequentially comprises a first meniscus lens, a first biconvex lens, a first biconcave lens, a second biconcave lens, a second biconvex lens, a second meniscus lens, a third biconvex lens, a third meniscus lens and a fourth meniscus lens from an object plane to an image plane. Wherein the first biconvex lens and the first biconcave lens form a first balsaming lens, the second biconcave lens and the second biconvex lens form a second balsaming lens, and the third biconvex lens and the third meniscus lens form a third balsaming lens; the first meniscus lens and the third meniscus lens are positive meniscus lenses, and the second meniscus lens and the fourth meniscus lens are negative meniscus lenses. The aperture diaphragm is disposed between the first biconcave lens and the second biconcave lens. According to the fixed-focus high-definition long-focus infrared confocal imaging optical system provided by the invention, large-target-surface high-definition imaging is realized only through nine lenses when the focal length is 35mm.

The invention relates to a whole genome multi-recombinant protein modification labeling method based on transposition reaction and fluorescence in situ hybridization and application thereof, and belongs to the technical field of cell molecular markers. The invention relates to a marking method modified by a whole genome multi-recombinant protein. The marking method comprises the following steps: cleaning cells carrying one or more special sequences for coding oligonucleotides; and mixing the cleaned cells, an EC buffer solution and an aqueous solution of the oligonucleotide fluorescent probe, carrying out room temperature incubation, discarding the liquid, cleaning, and carrying out fluorescence imaging. According to the labeling method, fluorescence in-situ labeling can be carried out on different types of fine histone modifications in the same cell sample, and the labeling method is suitable for an ultrahigh-resolution microscopic imaging platform or a common confocal imaging system. The marker has extremely high specificity and sensitivity, and spatial distribution and co-localization information of various histone modifications in a cell nucleus can be obtained.

This invention discloses an illumination intensity modulation method for disk scanning confocal imaging, comprising the following steps: S1: Before formal imaging, a pre-imaging is performed using weak, uniform illumination; S2: The fluorescence intensity of different regions in the pre-imaging is evaluated and analyzed, and the evaluation results are obtained; S3: Based on the evaluation results, the parameter settings of the intensity modulation mechanism are adjusted to achieve different illumination in different regions of the sample; S4: The scanning points traverse the imaging area to obtain a complete image on the camera. This invention can dynamically adjust the illumination intensity of local regions in the scanning imaging as needed, and can be used to reduce photobleaching in local regions, increase the dynamic range of imaging, and enhance the signal-to-noise ratio of local regions.

The present invention proposes a basic structural principle of a confocal imaging device based on completely equal optical path for adjacent surfaces. In the imaging optical paths of adjacent double surfaces, glass optical wedges are respectively used to implement a new detection device and method for spatial separation of double-sided imaging of semiconductor grains. This new device can obtain completely equal optical path confocal imaging detection for adjacent double surfaces of semiconductor grains simultaneously, and does not require the use of polarization optical elements and polarization CMOS sensors (cameras), effectively reducing the cost of the detection device.

The invention relates to the technical field of microscopy optics, in particular to a spot scanning confocal adjusting system and method, light emitted by a light source is parallel light beams, the divergence angle is smaller than 1mrad, the light beams reach a galvanometer through a spectroscope, reach a target object through a scanning mirror set and an objective lens, return after being reflected by the target object, pass through the spectroscope capable of rotating, and then pass through a scanning mirror set. After being reflected, the laser reaches the confocal hole through the focusing lens; the confocal hole is located at the rear focus of the lens, and confocal imaging is achieved. According to the invention, through the rotatable spectroscope, the imaging condition of the target object at the confocal hole position can be observed by one camera at the same time. The technical problem that the position of the confocal hole is difficult to find if the initial position of the confocal hole is not within the view field range of the camera due to the fact that the confocal hole is small, a camera with a high amplification factor needs to be selected for clearly seeing the confocal hole and the image plane range which can be observed by the camera is very small in the debugging process is solved.

The present invention discloses a high-precision spectral confocal microscopy imaging system, comprising a light modulation module, a spectrum splitting module, a scanning imaging module, an image detection device and a computer. The light beam emitted by the light modulation module passes through the scanning imaging module and is incident on a sample to excite a fluorescence signal. The fluorescence signal passes through the spectrum splitting module and is received by the image detection device. The image detection device converts the current signal into a digital signal and transmits it to the computer to complete imaging. The system is characterized in that the spectrum splitting module consists of a first converging lens, a first pinhole, a first linear long-wave pass filter, a second converging lens, a first linear short-wave pass filter and a third converging lens arranged in sequence. The system has the advantage of realizing high-precision spectral confocal imaging by combining lenses and linear filters on the basis of the traditional confocal microscopy imaging principle, solving the problem of spectral crosstalk in confocal imaging, and achieving a spectral resolution of less than 3.0 nm.

The application discloses a coaxial spectrum confocal imaging system, comprising a light source module, a dispersion lens module, a spectrum imaging side slit device and a spectrometer; the light source module provides a wide-spectrum light beam and makes the light beam enter the dispersion lens module through a light source side slit device; the dispersion lens module makes the incident light sequentially pass through a first dispersion lens module, a light splitting device and a second dispersion lens module, and then is shot to a surface of a measured object, and also makes the light reflected by the surface of the measured object sequentially pass through the second dispersion lens module, is reflected by the light splitting device and passes through a third lens module, and then is converged to the spectrum imaging side slit device; the spectrometer images the light of different wavelengths converged to the spectrum imaging side slit device. The application overcomes the shielding problem of the prior art when measuring a relatively steep pit or hole by the mode that the incident light path and the reflected light path share a second dispersion lens module coaxially, and realizes the measurement of a deeper pit on the surface.

The utility model discloses a multi-cavity endoscope capable of simultaneously performing biopsy and confocal imaging, which comprises an endoscope main body, a fixing plate is arranged on the outer side of the endoscope main body, an anti-closing component is arranged on the fixing plate, a positioning component is arranged in the fixing plate, and the endoscope main body is provided with a cavity. The anti-closing part comprises cavities formed in the two sides of the interior of the fixing plate respectively, a rotating shaft connected with the cavities, a driving bevel gear fixedly connected with the outer side of the rotating shaft and driven bevel gears connected with the two sides of the driving bevel gear respectively. The problems that when an existing endoscope is used, although a human body can be examined, during examination, a patient needs to open the mouth, then the endoscope is put into the body of the patient, due to the fact that the endoscope has a certain size, the patient feels uncomfortable, the mouth is prone to being closed, movement of the endoscope is affected, the examination efficiency is affected, and the examination efficiency is affected are solved. And therefore, the practicability is reduced.

A porphyrin compound for bioimaging and sonodynamic therapy, as well as its preparation method and application, has a chemical formula shown in Formula 2. The preparation method comprises the following steps: adding p-methylthiobenzaldehyde, a compound of Formula 1, and pyrrole to a reaction flask, evacuating the flask and introducing nitrogen gas, followed by adding anhydrous dichloromethane to obtain a reaction solution; bubbling nitrogen gas through the reaction solution, stirring at 25°C, adding boron trifluoride etherate, maintaining the temperature while continuing to stir, adding DDQ, continuing stirring, adding triethylamine, filtering, concentrating, and purifying to obtain the target product. This method features mild reaction conditions, a short reaction time, and simple operation. The resulting porphyrin compound exhibits excellent sonosensitivity and biocompatibility, can efficiently generate reactive oxygen species under ultrasound, and can produce excellent sonodynamic effects. The compound also exhibits fluorescence emission in the near-infrared region and can be excited by visible light, enabling confocal imaging to detect changes in cell morphology before and after, resulting in excellent bioimaging results. #imgabs0#

An apparatus for optically analyzing a sample may include an imaging subsystem that images the sample, one or more analyzing subsystems that analyze the sample including a confocal imaging subsystem, a temperature control subsystem that controls a temperature of the atmosphere within the apparatus, a gas control subsystem that controls a composition of the atmosphere within the apparatus, and a control module that controls the various subsystems of the apparatus.

The invention discloses a wide-spectrum optical system and a lens, and relates to the technical field of optical imaging. The optical system comprises twelve lenses which are sequentially arranged from the object space to the image space, the fifth lens and the sixth lens form a first balsaming lens, and the seventh lens and the eighth lens form a second balsaming lens; the ninth lens, the tenth lens and the eleventh lens form a third cemented lens. The second lens, the third lens, the sixth lens, the seventh lens, the ninth lens and the eleventh lens are lenses with positive focal power, the twelfth lens is a plane lens, and the others are lenses with negative focal power. According to the wide-spectrum optical system and the lens provided by the invention, through a special optical structure consisting of twelve lenses, dispersion aberration and geometric aberration in a range of 400-1700 nanometers can be corrected at the same time, so that high-precision confocal imaging is realized, and the performance of a spectrum imaging system is remarkably improved.

The subject invention pertains to compositions of a probe comprising two pH-reversible forms, including the boron dipyrromethene (BODIPY) fluorescent form and the non-fluorescent leuco-BODIPY form, for use in confocal imaging methods of intracellular vesicles as well as extracellular vesicles that are present in conditioned culture medium and / or secreted by living cells, without inducing severe cytotoxicity.

The application provides a single-objective multi-mode microscopic imaging system, and relates to the technical field of microscopic imaging.The system comprises a laser light source, an excitation light path module, a modulation scanning module, a detection light path module, an imaging camera and a controller.The excitation light path module comprises an expansion-constriction lens group, a shaping unit, a first slit and a first relay lens group.The modulation scanning module comprises a dynamic light splitting unit in a non-fixed position, a first scanning galvanometer, a scanning lens, a barrel lens and an objective lens.The detection light path module comprises a first lens, a second relay lens group, a second slit in a non-fixed state, an electronically adjustable lens, a third relay lens group and a second scanning galvanometer.The modulation scanning module is used for transmitting a laser beam to the back focal plane of the objective lens and relaying sample fluorescence collected by the objective lens to the detection light path module.The controller is used for switching between oblique light sheet imaging mode and confocal imaging mode according to the position of the dynamic light splitting unit and the state of the second slit.

The invention relates to a skin in-vivo confocal imaging image brightness adaptive adjusting device and method, and the method comprises the steps: employing a scanning imaging device to emit illumination light to a skin sample, and carrying out the point-by-point scanning imaging of the skin sample through a micro-electromechanical system in a point-by-point scanning mode; and the brightness of the illumination light is adaptively adjusted in an iterative mode through the brightness adjusting device. In the iteration process, the average signal intensity of the electric signals of the obtained imaging result in the sampling period and the number of the pixels with the signal intensity higher than the reference value are substituted into an empirical formula, an adjusting parameter value for adjusting the brightness of the illumination light in the next step is obtained, and then the brightness adjustment in the next step is carried out. Therefore, according to the self-adaptive adjusting device disclosed by the invention, the light emitting power of the light source does not need to be frequently and manually adjusted or the signal dynamic range in a software system does not need to be manually changed, special image processing is not needed, and the sample detection efficiency and the imaging effect can be improved.

The utility model discloses a device capable of simultaneously carrying out tissue cell collection and confocal imaging, which comprises a workbench and a puncture needle, one side of the workbench is provided with a collection detection mechanism, the collection detection mechanism comprises a transverse plate, one side of the transverse plate is fixedly connected with a hollow block, and the hollow block is fixedly connected with the puncture needle. An electric telescopic rod is fixedly connected to the front face of the hollow block, a connecting frame is fixedly connected to one side of the electric telescopic rod, and a sealing plate is fixedly connected to one side of the connecting frame. By arranging a collecting and detecting mechanism, starting an electric telescopic rod, driving a connecting frame to move by the electric telescopic rod, and driving a sealing plate to move by the connecting frame, negative pressure suction work is performed, cells are sucked into an inner cavity of a hollow block, and after part of the cells are sucked, the electric telescopic rod can be controlled to contract, so that the cells are pushed into an inner cavity of a fixed pipe through the sealing plate; cells are discharged to the top of the bearing plate through the fixing pipe, meanwhile, the bearing plate is moved to a detection position, and detection work is conducted through the detection probe.

A multi-depth confocal imaging system includes at least one light source configured to provide excitation beams and an objective lens. The excitation beams are focused into a sample at a first plurality of focus depths along an excitation direction through the objective lens. An image sensor receives emissions from the sample via the objective lens, wherein the emissions define foci relative to the image sensor at a second plurality of focus depths.

The endoscope catheter comprises a catheter body, a side-view imaging assembly and an end cap, a confocal channel and an operation channel are arranged in the catheter body, the end cap comprises a light emitting part and a connecting part, the light emitting part protrudes out of the connecting part so that the end cap can be in a step shape, and the side-view imaging assembly is arranged on the side-view imaging assembly. The end cap is arranged at the far end of the catheter body in a sleeving mode, and the side-view imaging assembly is located at the far end in the confocal channel. The confocal probe enters the human body through the confocal channel to carry out confocal imaging on target tissue, when side-view imaging is carried out, the far end of the confocal probe can be located at the light emitting part in the end cap, and meanwhile, the far end of the confocal probe is matched with the side-view imaging assembly at the far end in the confocal channel to carry out side-view imaging on the target tissue. The confocal probe located in the protruding light emitting part can collect emitted light on the side face and cannot be blocked by surrounding light, and therefore side-view imaging is achieved.

The invention discloses a pressurized constant-temperature incubator for three-dimensional culture of stem cells and a cell culture method, and relates to the technical field of three-dimensional culture of stem cells. The environment control structure comprises a closed-loop gas circulation flow channel arranged in the incubator main body, and through the synergistic effect of a gas circulation driving mechanism and a mechanical stimulation assembly, the complex mechanical environment of in-vivo multi-physical field coupling is accurately simulated; meanwhile, osmotic pressure change and chemical stress caused by traditional liquid perfusion are perfectly avoided by utilizing closed-loop gas circulation. The piezoelectric ceramic actuator group supports high-flux and personalized research, a time-sharing working strategy realizes interference-free in-situ dynamic monitoring of mechanical stimulation and high-precision confocal imaging, and a transmission control assembly further realizes intelligent linkage of airflow guiding and electromagnetic buffering adjustment, so that the stimulation accuracy and purity are ensured, the overall high integration and automation are realized, and the mechanical stimulation and high-precision confocal imaging are realized. And reliable data support is provided for three-dimensional culture of stem cells.

The present invention discloses a day-night confocal lens and an imaging device. The day-night confocal lens includes a first convex-concave lens with a negative optical power, a second double-convex lens with a positive optical power, a third double-convex lens with a positive optical power, and a fourth convex-concave lens with a negative optical power, which are arranged in sequence from the object side to the image side. The total length of the day-night confocal lens is controlled within 22.4 mm, the aperture value F satisfies 1.6 ≤ F ≤ 2.0, it supports an image plane of 1 / 2.7 inch, and can also achieve clear imaging in low light. By reasonably reducing the number of lenses, the day-night confocal lens can achieve low cost and light weight. Through reasonable setting of the optical powers and shapes of the four lenses, an FNO 1.6 large aperture can be achieved, and at the same time, the resolution can reach 4M, ensuring high resolution under a large aperture, so as to provide a low-cost, large-aperture, small-sized day-night confocal lens capable of day-night confocal imaging.

The utility model provides a multi-mode fluorescence microscope. The multi-mode fluorescence microscope comprises a microscopic assembly, a near-infrared two-region excitation light path, a visible light confocal excitation light path, a wide-field imaging light path, a near-infrared two-region confocal imaging assembly, a multi-photon intensity / life harmonic wave imaging assembly and a visible light confocal and wide-field imaging assembly, the microscopic assembly comprises an objective lens, an optical filter switching assembly, a sleeve lens and a transflective device which are sequentially arranged from the object side to the image side, and the transflective device can be switched to a dichroscope or a reflector; the near-infrared second-area excitation light path, the visible light confocal excitation light path and the wide-field imaging light path are connected with the sleeve lens; the near-infrared two-region confocal imaging assembly, the multi-photon intensity / lifetime and harmonic imaging assembly and the visible light confocal and wide-field imaging assembly are all connected to the sleeve lens. According to the scheme, confocal and wide-field imaging of visible light, confocal and wide-field imaging of a near-infrared second region, and multi-photon intensity / lifetime and harmonic imaging based on near-infrared excitation are realized.

The utility model discloses a confocal perforated plate for tube formation experiment, which comprises a base plate structure, accommodating hole structures and bulge structures, the accommodating hole structures are equidistantly arranged on the upper surface of the base plate structure, and the bulge structures are arranged at the bottoms of the inner walls of the accommodating hole structures. According to the confocal perforated plate for the tube forming experiment, self-leveling of matrigel is achieved through combination of micron-sized annular protrusion arrays designed at the bottoms of the holes and super-hydrophilic surface modification, the standard deviation of the thickness of a gel layer is controlled within + / -3 microns, and the height difference of the liquid level is reduced to lt through side wall gradient hydrophobic treatment (top hydrophobic / bottom hydrophilic); 0.1 mm, and halo artifacts in confocal imaging are eliminated.