The application discloses a line illumination modulation multicolor imaging
system, belonging to the technical field of multicolor imaging, comprising: a line illumination modulation module, comprising m monochromatic light sources and a modulation light path, the
wavelength of the
light beam emitted by each monochromatic
light source is different; each
light beam passes through the modulation light path to form a line
light spot focused on the focal plane of an objective lens, the illumination intensity of the line
light spot on the focal plane of the objective lens is in
Gaussian distribution in a first direction, the position parameters of each line
light spot are different, and the line light spots of the m channels are superimposed to form m-color line illumination light; an imaging module is used for continuously scanning imaging along the first direction by using a multi-element
detector with n rows of pixels, and at least m mixed images under the
irradiation of the line illumination light are obtained, each mixed image corresponds to one row of pixels, and each mixed image contains signals of the m channels; and an image
demodulation module is used for demodulating monochromatic images from the mixed images, and each monochromatic image corresponds to the signals of one channel. The application realizes synchronous multicolor imaging on a single
detector.