Multi-mode co-focusing imaging method and apparatus

Abstract

The invention discloses a multi-mode confocal imaging method and a device thereof. The method comprises the following steps: transmitted light sources of two signal acquiring devices are combined by dichroic beamsplitters and projected onto a target tissue; reelected signal light of the target tissue is separated by the dichroic beamsplitters into two beams which reach respective filter elements to filter interference signals and then reach respective detection elements to perform synchronous imaging process to the detection signals of a sample. The device comprises a reflected signal acquiring device, a fluorescence signal acquiring device, an optical path of the two devices for light combination consisting of a first dichroic beamsplitter, a scanning mirror and an object lens, and a detection light path of the reflected signal acquiring device and a detection light path of the fluorescence signal acquiring device, wherein each detection light path comprises a filter plate, a lens, a confocal pinhole and a photoelectric detector. The photoelectric detectors are connected with an imaging computer system. Two confocal acquisition systems acquire a signal at the same time and position, and an optical design is adopted to avoid mutual interference of multi-path laser light and the detection systems, thereby realizing real-time noninvasive 3D detection of biological tissue.

Description

technical field

[0001] The present invention relates to a method for optical imaging, in particular to a method for performing multi-mode confocal optical imaging using reflection and fluorescence, which is mainly suitable for multi-mode confocal imaging of biological tissues;

[0002] The invention also relates to an optical imaging device, in particular to a multi-mode confocal optical imaging device using reflection and fluorescence. Background technique

[0003] The confocal reflective imaging device proposed in the prior art [1] (see Opt. Photonics News, P. Daucatas, 18, 2007, pp. 28-33) utilizes reflected light from tissues to perform imaging. Since the reflected signal is only sensitive to the refractive index, selective imaging of specific biomolecules cannot be achieved.

[0004] Described in the prior art [2] (see J. Microsc., A. L. Carlson et al., 228, 2007, pp. 11-24) is a confocal reflection and fluorescence dual-mode imaging method. The basic principle is to ...

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