The microscope has a first pulsed laser generating means, a second pulsed laser generating means, an irradiation means to irradiate to a specimen by composing the first pulse light and the second pulse light, a coherent Raman scattering light extraction means for extracting only the coherent Raman scattering light from light emanated from the specimen irradiated, an extraction means for extracting only the multiphoton excitation fluorescence, an extraction means for extracting only the second harmonic wave, a detection means for detecting the extracted coherent Raman scattering light, and a detection means for detecting the extracted fluorescence, and a detection means for detecting the second harmonic wave via the extraction means 7. To single specimen, responding to purposes, observations of the two-photon excitation fluorescence observation, the second harmonic wave observation, and the coherent Raman scattering light observations can be carried out in parallel, or selectively.