Functional fluorescence carbon nanoparticles based on natural saccharide materials and preparation method and application thereof

A carbon nanoparticle and nanoparticle technology, applied in the field of nano-fluorescent materials, can solve the problems of complex preparation process, not extensive enough, and difficult separation of carbon nanoparticle, and achieve the effect of simplifying complex synthesis process, expanding selection range, and good dispersibility.

Inactive Publication Date: 2013-10-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current preparation process of carbon nanoparticles is relatively complicated, the exploration of carbon sources is not extensive enough, and the resulting carbon nanoparticles are difficult to separate and other issues need to be further resolved

Method used

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  • Functional fluorescence carbon nanoparticles based on natural saccharide materials and preparation method and application thereof
  • Functional fluorescence carbon nanoparticles based on natural saccharide materials and preparation method and application thereof
  • Functional fluorescence carbon nanoparticles based on natural saccharide materials and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation and separation and purification of carbon nanoparticles

[0031] (1) Preparation of cel-CNPs, chi-CNPs, sta-CNPs, cyc-CNPs carbon nanoparticles

[0032] Weigh 1 g of starch (cellulose, chitosan or cyclodextrin) and 0.1 g of NaOH respectively in a beaker, and add 15 ml of deionized water. Then the reactants were placed in an autoclave for 4 hours at a temperature of 160 degrees. After cooling to room temperature, the pH of the reaction product was adjusted to 7 with 1M HCl.

[0033] (2) Separation and purification of cel-CNPs, chi-CNPs, sta-CNPs, cyc-CNPs carbon nanoparticles:

[0034] Use a centrifuge to remove impurities from the obtained product solution, first use 4000rmp to remove larger impurity molecules in the product solution, and then use mixed cellulose material with 0.8μm, 0.45μm, and 0.22μm microfiltration membranes to remove relatively small impurities. of impurities. Then the obtained sample solution was treated with a dialysis b...

Embodiment 2

[0035] Example 2: Characterization of Carbon Nanoparticle Properties

[0036] (1) TEM characterization of carbon nanoparticles

[0037] Depend on figure 1 It can be seen that the particle size of cel-CNPs is about 150nm, the particle size of chi-CNPs is about 100nm, and the particle size distribution is relatively uniform, and the monodispersity is better; the particle size of sta-CNPs and cyc-CNPs is about 100-200nm , the particle size distribution is wider, but the monodispersity is better.

[0038] (2) UV absorption spectrum and fluorescence spectrum of carbon nanoparticles

[0039] figure 2 , image 3 , Figure 4 and Figure 5 The UV absorption spectra and fluorescence spectra of cel-CNPs, chi-CNPs, sta-CNPs, and cyc-CNPs are shown respectively. It can be seen from the figure that the four carbon nanoparticles have similar absorption peaks at 250-300nm. It is a typical peak of C=C. Under the action of excitation light of different wavelengths (300nm-500nm), the fou...

Embodiment 3

[0044] Example 3: Microwave treatment improves the fluorescence quantum yield of carbon nanoparticles

[0045] (1) Microwave treatment method

[0046] Take a certain amount of carbon nanoparticles (sta-CNPs, cel-CNPs, chi-CNPs, cyc-CNPs), add deionized water to 50ml, then put it in the center of the microwave oven, heat it at 800W for 5 minutes, and set it with ultrapure water after cooling. to its original volume.

[0047] (2) Fluorescence quantum yields of four carbon nanoparticles after microwave treatment

[0048] Table 1 shows the fluorescence quantum yields of four kinds of carbon nanoparticles before and after microwave. The quantum yields of carbon nanoparticles with different carbon sources are different, and the quantum yields of nanoparticles obtained with starch as carbon source are higher. In addition, the quantum yields of nanoparticles obtained from the same carbon source are also significantly different under different excitation light. Using cellulose and c...

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Abstract

The invention discloses a preparation method of functional fluorescence carbon nanoparticles (CNPs) based on natural saccharide materials and representation and application thereof in measurement for biotechnique. The preparation method is characterized by comprises the following steps of dissolving 1g of carbon sources (cellulose, starch, chitosan and cyclodextrin) and 0.1g of NaOH into 15ml of deionized water, then reacting reacting for 4 hours in an autoclave at 160 DEG C, and carrying out acid-base neutralization and microwave treatment on the obtained carbon nanoparticles. According to the preparation method, the raw materials are abundant and cheap and are easily available, the preparation method is simple, and the synthesized nanoparticles have good fluorescence characteristic and the characteristics of stable fluorescence, dark flashing, tunable wavelength, wide excitation spectra, narrow emission spectrum and the like, and can be used as a fluorescence indicator in the biological medicine field.

Description

technical field [0001] The invention relates to nano fluorescent materials, specifically the preparation, characterization and application of carbon nano particles based on four kinds of natural polysaccharides. Background technique [0002] Fluorescent materials have received more and more attention in the fields of biotechnology and optical materials. Among them, fluorescent labels have been greatly developed in many application fields such as biological detection, sensing, and imaging. It is against this background that the research on fluorescent nanoparticles has gradually attracted the attention of researchers at home and abroad, and has developed rapidly in recent years. Its application focus is currently mainly focused on the application of fluorescence microscope detection, cell labeling, DNA labeling and in vivo imaging. The reported technology and its existing problems include the following aspects: [0003] (1) Fluorescent nanoparticle ZnS-CdSe (also known as q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B31/02G01N33/52C01B32/15
Inventor 马小军谭明乾颜惠平
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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