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Functional carbon nanoparticles and application thereof

A carbon nanoparticle and functional technology, applied in the field of nano-fluorescent materials, can solve the problems of difficult separation of carbon nanoparticle, complicated preparation process and not enough extensiveness, etc. Effect

Inactive Publication Date: 2014-06-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current preparation process of carbon nanoparticles is relatively complicated, the exploration of carbon sources is not extensive enough, and the resulting carbon nanoparticles are difficult to separate and other issues need to be further resolved

Method used

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  • Functional carbon nanoparticles and application thereof
  • Functional carbon nanoparticles and application thereof
  • Functional carbon nanoparticles and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Preparation and separation and purification of carbon nanoparticles

[0027] (1) Preparation of Alg-CNPs:

[0028] Weigh 1g of sodium alginate and 0.1g of NaOH into a beaker, and add 30ml of deionized water. Then the reactants were placed in an autoclave for 4 hours at a temperature of 160 degrees Celsius. After cooling at room temperature, the pH of the reaction product was adjusted to 7 with 1M HCl.

[0029] (2) Separation and purification of Alg-CNPs:

[0030] The resulting product solution was treated with 0.45 μm and 0.22 μm microfiltration membranes to remove macromolecular impurities. Then use a high-speed desktop refrigerated centrifuge (10000rpm) to centrifuge for 20 minutes, and then use a dialysis bag (500Da) to remove small molecules such as salt. The dialysis time is 2 days (change the water every 2 hours).

Embodiment 2

[0031] Example 2: Characterization of Carbon Nanoparticle Properties

[0032] (1) Transmission electron microscope characterization of carbon nanoparticles:

[0033] Depend on figure 1 It can be seen that the particle size of sodium alginate carbon nanoparticles is about 100nm, and the particle size distribution is relatively uniform, and the monodispersity is good.

[0034] (2) UV absorption spectrum and fluorescence spectrum of carbon nanoparticles

[0035] figure 2 It is the ultraviolet absorption spectrum and fluorescence spectrum of alg-CNPs after reduction. It can be seen from the figure that carbon nanoparticles have an absorption peak at 260nm, which is a typical peak of C=C. Under the action of excitation light of different wavelengths (320nm-420nm), carbon nanoparticles emit different wavelengths of light, mainly concentrated in the visible light range. As the excitation wavelength increases, the emission wavelength is red-shifted.

[0036] (3) Fourier transfor...

Embodiment 3

[0040] Example 3: Sodium borohydride treatment improves the fluorescence quantum yield of carbon nanoparticles

[0041] (1) Sodium borohydride treatment

[0042] Put 15ml of carbon nanoparticles into a beaker, add 1.5g of sodium borohydride and mix well, then use dialysis to remove excess sodium borohydride (500Da), and the dialysis time is 2 days (change the water every 2h).

[0043] (2) Fluorescence quantum yield of carbon nanoparticles after sodium borohydride treatment

[0044] It can be concluded from Table 1 that the quantum yield of the synthesized carbon nanoparticles is greatly improved after sodium borohydride reduction.

[0045] (3) Effect of pH value on the fluorescence properties of carbon nanoparticles

[0046] Take 0.5ml of alg-CNPs samples, add 4.5ml of deionized water, and adjust the pH to 3, 5, 7, 9, 11, 13 with 5M HCl and 5M NaOH to do three parallel experiments. Figure 5 is the effect of pH value on the fluorescence characteristics of carbon nanoparticl...

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Abstract

The invention discloses a preparation and characteristic of functional fluorescent carbon nanoparticles (referred to as CNPs) and an application thereof in bio-detection techniques. The preparation is characterized in that 1g of sodium alginate and 0.1g of NaOH are mixed and slowly added to 30ml of deionized water and thoroughly stirred and then placed in an autoclave and reacted for 4h at 160 DEG C. The carbon nanoparticles obtained by the reaction are subjected to acid-base neutralization. The nanoparticles have rich source of raw materials, cheap and easily available raw materials, and simple preparation method; synthesized carbon nanoparticles have good fluorescence properties including fluorescent stability, no light flashes, controllable excitation and emission wavelength, good biocompatibility, low toxicity, very small molecular weight and particle size, and have rapid development in biological detection, sensing, angiography, and many other application fields.

Description

technical field [0001] The invention relates to a nano fluorescent material, in particular to the preparation, characterization and application of a carbon nano particle. Background technique [0002] In 2004, researchers at Clemson University accidentally discovered carbon nanoparticles during electrophoretic separation of multi-walled carbon nanotubes. Its main constituent element is carbon, which is one of the most abundant elements in nature and one of the most important constituent elements of life. Due to its non-toxicity and good fluorescence properties, it is favored and widely used in many fields. It is mainly used for labeling and in vivo imaging of microorganisms and animal cells. The reported technology and its existing problems include the following aspects: [0003] (1) Light-emitting semiconductor quantum dot (also known as quantum dot) technology (Document 1: Warren C.W.Chan and ShumingNie, Science, 1998, 281, 2016-2018). The invention of quantum dots has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02C09K11/65C01B32/15
Inventor 马小军谭明乾颜惠平
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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