Rapid propagation method adopting induction of cotyledon somatic embryos of Chinese scholartree

A technology of somatic embryos and cotyledons of Sophora japonica, applied in the field of plant biology, can solve the problems of low regeneration rate of Sophora japonicae, and achieve the effect of saving time.

Active Publication Date: 2016-06-08
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is exactly in order to solve the above-mentioned problem, provides a kind of method for the rapid propagation of Chinese Sophora japonica cotyledon somatic embryo induction, to solve the problem of the low regeneration rate of Chinese Sophora japonica

Method used

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  • Rapid propagation method adopting induction of cotyledon somatic embryos of Chinese scholartree
  • Rapid propagation method adopting induction of cotyledon somatic embryos of Chinese scholartree
  • Rapid propagation method adopting induction of cotyledon somatic embryos of Chinese scholartree

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Experimental program
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Effect test

Embodiment 1

[0043] Select the mature seeds of Sophora japonica that are healthy and free from diseases and insect pests, remove the fruit pods, wash carefully with detergent, rinse with running water for 1 hour, filter paper to absorb excess water outside the seeds, and prepare for disinfection.

[0044] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , Gently peel off the seed coat with a scalpel blade and tweezers, and take out the cotyledons. Gently separate the two cotyledons with tweezers and a blade, keep the germ, then place the cotyledon with the flat side down and the convex side up in a petri dish, and use a scalpel to make 3-4 cuts perpendicular to the main vein of the convex side of the cotyledon. Take care not to scratch the edge of the cotyledon.

[004...

Embodiment 2

[0055] Select the mature seeds of Sophora japonica that are healthy and free from diseases and insect pests, remove the fruit pods, wash carefully with detergent, rinse with running water for 1 hour, filter paper to absorb excess water outside the seeds, and prepare for disinfection.

[0056] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 10 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , Gently peel off the seed coat with a scalpel blade and tweezers, and take out the cotyledons. Gently separate the two cotyledons with tweezers and a blade, keep the germ, then place the cotyledon with the flat side down and the convex side up in a petri dish, and use a scalpel to make 3-4 cuts perpendicular to the main vein of the convex side of the cotyledon. Take care not to scratch the edge of the cotyledon.

[00...

Embodiment 3

[0066] Select the mature seeds of Sophora japonica that are healthy and free from diseases and insect pests, remove the fruit pods, wash carefully with detergent, rinse with running water for 1 hour, filter paper to absorb excess water outside the seeds, and prepare for disinfection.

[0067] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 12 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , Gently peel off the seed coat with a scalpel blade and tweezers, and take out the cotyledons. Gently separate the two cotyledons with tweezers and a blade, keep the germ, then place the cotyledon with the flat side down and the convex side up in a petri dish, and use a scalpel to make 3-4 cuts perpendicular to the main vein of the convex side of the cotyledon. Take care not to scratch the edge of the cotyledon.

[00...

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Abstract

The invention discloses a rapid propagation method adopting induction of cotyledon somatic embryos of Chinese scholartree. The rapid propagation method comprises the following steps: selecting current-year-grown mature seeds without diseases and insect pests of the Chinese scholartree and disinfecting; then taking out cotyledons and cutting; inoculating the cotyledons on a somatic embryo culture medium and culturing to form calluses; transferring the calluses on an adventitious bud induction culture medium and culturing to form cluster bud plantlets; cutting the cluster bud plantlets into callus blocks and transferring the callus blocks to a test-tube plantlet proliferation culture medium or an adventitious bud induction culture medium and culturing; and transferring the cultured cluster buds into a strong seedling culture medium and carrying out strong seedling culture, and then hardening the seedlings and transplanting. The method disclosed by the invention is high in regeneration rate and has more buds; and the transplanting survival rate of plants can reach more than or equal to 90%, germchits grow healthy and the germplasm degeneration problem of the high-quality germchits can be effectively solved.

Description

technical field [0001] The invention relates to a rapid propagation method induced by cotyledonary leaf somatic embryos of Chinese pagoda japonica, belonging to the field of plant biotechnology. Background technique [0002] Sophora japonica Linn, also known as Sophora japonica Linn, Chinese Sophora japonica, belongs to Papilionaceae, Sophora genus, is an ideal street tree species and garden greening tree species. Moreover, the material of Sophora japonica is excellent, the flowers, fruit, root bark, and bark can be used as medicine, and the seeds can be squeezed to make soap. It has high economic value and has a very broad application prospect. At the same time, the national pagoda tree is the only rootstock tree species for grafting ornamental species such as Sophora japonica, Jinzhi Huai, Liaohong Huai, and Butterfly Huai, and there is a large demand for seedlings. Sophora japonica is mainly propagated by seeds, but its flowering and fruiting have the phenomenon of large...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 庞彩红李双云孙超王因花毛秀红刘盛芳王学文夏阳
Owner SHANDONG FOREST SCI RES INST
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