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35 results about "Scalpel blade" patented technology

Combinable surgical blade

The utility model relates to the technical field of medical surgery, in particular to a combinable surgical blade which comprises a hollow knife handle, a fixing block, clamping pieces and a knife body, the fixing block is arranged in the hollow knife handle in a sleeved mode, the two sides of one end of the fixing block are connected with the clamping pieces through connecting rods, limiting holes are formed in the clamping pieces at equal intervals, and the knife body is connected with the hollow knife handle in a sleeved mode. A knife body main body is clamped between the clamping pieces, and limiting convex blocks are welded to the two sides of the knife body main body at equal intervals and located in the limiting holes; a blade is arranged at one end of the bottom of the knife body, and a knife point is arranged at the top end of the blade. The scalpel blade is convenient to assemble and disassemble quickly, the clamping stability of the scalpel body is good, the problem that a traditional scalpel blade is clamped in a limiting through groove in the middle of the scalpel body through a clamping plate and is prone to sliding is solved, subcutaneous elastic adhesion tissue can be sawed off through sawteeth at the top of the scalpel body, and the using effect is improved.
Owner:HUAIAN HAILUN MEDICAL EQUIP CO LTD

Scalpel blade (III)

1. Name of the Design Product: Surgical Blade Head (III). 2. Use of the Design Product: For coagulation and resection of tissues during surgical operations. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.
Owner:HUNAN JINGYI MEDICAL TECH CO LTD

Blade assembly and multi-degree-of-freedom ultrasonic scalpel

This invention discloses a blade assembly and a multi-degree-of-freedom ultrasonic scalpel. The blade assembly includes a housing assembly, a transducer, and a blade body. The housing assembly has a mounting cavity; the transducer is located within the mounting cavity and is adapted to be connected to the control board of the ultrasonic scalpel via a cable. The transducer is integrated into the housing assembly to reduce the overall size of the ultrasonic scalpel; one end of the blade body is inserted into the mounting cavity and connected to the transducer. This invention improves the structure of the ultrasonic scalpel blade assembly. By integrating the transducer into the blade assembly, a rigid structure is avoided in the connection between the blade assembly and the base. This improves the operational flexibility and operating space of the ultrasonic scalpel, enabling the ultrasonic blade to have multiple degrees of freedom and ensuring efficient energy transfer between the transducer and the blade.
Owner:SUZHOU WEISENTE MEDICAL ROBOT CO LTD

Pulse separator for scalpel

The utility model discloses a scalpel pulse separator. The technical key points are as follows: the device comprises a base and a pulse mechanism arranged on the base, the base is arranged on a rack, a through hole is formed between the base and the pulse mechanism, a collecting rod is arranged on the rack, the collecting rod is located below the pulse mechanism, the pulse mechanism comprises a relay and a separating rod arranged on the relay, and the separating rod is connected with the relay. A clamping groove is formed in the base, a clamping strip is connected into the clamping groove in a sliding mode, a plurality of supporting parts extending towards one side of the collecting rod are arranged on the clamping strip, the shapes of the supporting parts are different, the supporting parts are matched with the surgical blades of different specifications, the surgical blades of different specifications can be machined, the separation process is stable, and the defective rate is low. And adjustment is convenient and efficient.
Owner:ZHEJIANG SUICHANG SHUANGJIAN MEDICAL EQUIPMENT CO LTD

Gingival scalpel blade

PCT designated stageWO2026152196A1Dental surgeryDental Procedure
1) The present invention relates to a gingival scalpel blade intended to provide precise control of the width and depth of the tissue to be removed. 2) Its standardised blades and integrated depth marker provide greater safety, efficacy and predictability in dental procedures. 3) This device reduces the duration of surgeries, minimises unnecessary tissue removal and protects adjacent structures, resulting in superior outcomes.
Owner:BRANDAO FLAVIO EDUARDO +1

Surgical blade dismantling device

The utility model relates to the field of medical instruments, in particular to a surgical blade dismantling device. The surgical knife blade dismantling device comprises a box body and a cover body matched with the box body, an inserting notch for inserting a surgical knife is formed in the cover body, a knife retracting notch corresponding to the inserting notch is formed in the box body, and a knife dismantling mechanism is arranged in the cover body. According to the surgical knife blade dismantling device, through the cooperation of the box body, the cover body, the inserting notch, the knife retracting notch, the knife dismantling mechanism and the like, when the surgical knife blade dismantling device is used, a surgical knife is inserted from the inserting notch, a surgical knife handle is pressed downwards, the knife dismantling mechanism dismantles a surgical knife blade, the surgical knife blade is left in the box body, and the surgical knife handle retreats from the knife retracting notch; the scalpel blade is conveniently detached in a sealed environment, so that the pollution risk and the accidental injury risk are greatly reduced, and the potential safety hazard is reduced.
Owner:郑万功

A method for determining the grinding wheel wear state of a multi-station scalpel grinding machine

The present application relates to the technical field of grinding wheel wear prediction of knife grinder, and discloses a kind of grinding wheel wear state determination method of multi-station scalpel blade grinding machine, collects acoustic emission source signal data, grinding power and abrasive grain height of grinding wheel, carries out pre-processing and obtains data set by feature extraction;Acoustic emission source signal and grinding power are used as the input variable of random forest model, and the abrasive grain height of the grinding wheel is used as the output variable, to establish RF detection model;The CPA algorithm is improved, and the optimal RF detection model is obtained by optimizing the hyperparameters of the RF detection model using ICPA;The optimal RF detection model is used to detect the grinding wheel wear state with the current collected acoustic emission source signal and grinding power as input, to obtain the abrasive grain height of the grinding wheel under the current state, and further obtain the wear state of the current grinding wheel.Compared with the prior art, the present application provides a kind of grinding wheel wear state determination method, so that the control system more simply obtains the feed amount of grinding wheel, thereby compensating the grinding wheel.
Owner:HUAIYIN INSTITUTE OF TECHNOLOGY

Sharps blade ejector mechanism

A sharps blade ejector mechanism for safely removing used sharps blade from various surgical instrument handles without the need for using additional blade remover devices, needle holder instruments, or physical contact with the sharps blade is disclosed. The sharps blade ejector mechanism comprises a specifically configured handle in coordination with a molded sharps blade unit. The handle may be illuminated. The sharps blade may be a scalpel blade, a needle, a probe, a dissector, an elevator, a saw blade, a retractor or a hook.
Owner:NANO SURGICAL INC

Joint revision surgical systems and methods

A joint revision surgical system is provided that includes: (a) an osteotome blade / surgical osteotome guide block assembly having: a centrally located cavity and a plurality of anterior osteotome blade guide slots; a shank trunnion fastening member mountable within the central cavity and fastened to the osteotome blade guide block; the L-shaped adjustable blade guide plate is movably positioned in the plurality of osteotome blades / surgical osteotome guide grooves positioned at the front part; and a plurality of posterior osteotome blade / surgical osteotome guide slots, the plurality of posterior osteotome blade / surgical osteotome guide slots for receiving an osteotome blade and a surgical osteotome; (b) a surgical ram assembly; (c) an osteotome blade / surgical osteotome locking handle assembly; and (d) a plurality of osteotome blades / surgical osteotomes including osteotome blades having flexible and rigid blade shafts; wherein the assembled osteotome blade / surgical osteotome guide block is attached to an implanted femoral stem to be removed by attaching the stem trunnion fastening member to a trunnion of the stem.
Owner:MAP MEDICAL SOLUTIONS LLC

Simple device for disinfecting and cooling caenorhabditis elegans scalpel

The invention discloses a simple device for disinfecting and cooling a caenorhabditis elegans scalpel. The simple device comprises a bottom bracket mechanism, three alcohol lamps, three overhanging supporting mechanisms and three elastic clamping mechanisms, the three alcohol lamps are respectively placed at three heating point positions on the bottom bracket mechanism and are positioned at different heights; the three overhanging supporting mechanisms are all rotationally installed on the bottom support mechanism and located at different height positions. The three elastic clamping mechanisms are fixed to the overhanging ends of the three overhanging supporting mechanisms correspondingly and used for elastically clamping the surgical blade. According to the simple device for disinfecting and cooling the scalpel, through cooperation of the three overhanging supporting mechanisms and the three elastic clamping mechanisms, stable clamping of the three scalpel blades can be achieved, manual handheld heating is not needed, and therefore the stability of heating and disinfecting of the scalpel blades is guaranteed, and the safety in the operation process can also be guaranteed.
Owner:NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A +1

Inclined positioning knife rest for scalpel

ActiveCN222640592Ueasy accessReduce liquid residueIncision instrumentsSurgical furnitureKnife bladesScalpel blade
The inclined positioning knife rest comprises a base plate and a knife holder, the knife holder is located on the upper end face of the base plate, and an inclined top face is arranged on the upper portion of the knife holder. A blade groove and a handle groove are formed in the inclined top surface; the blade groove and the handle groove are grooves with upward openings and are communicated with each other; the inner wall of the handle groove is provided with flexible convex ribs, and the flexible convex ribs are arranged in a linear array shape in the extending direction of the handle groove; a flow guide groove is further formed in the tool apron, located at the bottom end of the blade groove and communicated with the lower end of the handle groove. A liquid collecting groove is further formed in the base plate. According to the inclined positioning knife rest for the scalpel, the scalpel can be positioned and placed, medical staff can take and place the scalpel conveniently, the risk of touching the scalpel blade is reduced, and residual liquid on the scalpel blade and the scalpel handle can flow down.
Owner:阮娜娜

Folding knife with blade convenient to replace

ActiveCN222644663UThe connection is firmly fixedSimple structureMetal working apparatusSurgical bladeKnife blades
The folding knife with the blade convenient to replace comprises a surgical knife blade, a blade fixing seat and a handle, the surgical knife blade is detachably installed on the blade fixing seat, the tail end of the blade fixing seat is connected with the front end of the handle, the front side face of the blade fixing seat is provided with a containing groove for containing the surgical knife blade, a fixing assembly is arranged in the containing groove, and the handle is connected with the blade fixing seat. The blade fixing base is provided with a fixing assembly, the fixing assembly is matched with a blade groove of the surgical blade to fix the surgical blade, the blade fixing base is further provided with a locking assembly, and the locking assembly is provided with a first position and a second position which can move relative to the blade fixing base. The locking assembly is matched with the scalpel groove of the scalpel blade to limit the scalpel blade to move relative to the fixing assembly, and when the locking assembly is located at the second position, the scalpel blade can move relative to the fixing assembly. The surgical knife has the advantages that the surgical knife blade and the knife blade fixing seat are firmly connected and fixed, safety is good, the surgical knife blade can be disassembled, assembled and replaced by hands, and operation is easy and convenient.
Owner:YANGJIANG XINHAOHENG IND & TRADE CO LTD

Dismounting and sterilizing device for surgical blade

The utility model relates to the field of medical instruments, in particular to a surgical blade dismounting and sterilizing device. The device comprises a lower shell and an upper shell buckled with the lower shell, a scalpel insertion opening is formed in the lower end of the upper shell, and a scalpel unloading mechanism corresponding to the scalpel insertion opening in position is arranged in the lower shell; and a disinfectant bag for puncturing the scalpel is arranged in the upper shell. According to the surgical knife blade dismounting and sterilizing device, through cooperation of the lower shell, the upper shell, the surgical knife inserting opening, the knife dismounting mechanism, the disinfectant bag and the like, when the surgical knife blade dismounting and sterilizing device is used, a surgical knife is inserted from the surgical knife inserting opening, on one hand, the disinfectant bag can be scratched, and disinfectant enters the lower shell; on the other hand, the scalpel blade is detached through the scalpel detaching mechanism, and the scalpel blade is left in the lower shell and soaked in the disinfectant, so that the disinfection and sterilization effects are achieved, the scalpel blade is conveniently detached in a sealed environment, the accidental injury risk is greatly reduced, and potential safety hazards are reduced.
Owner:胡杰

Surgical knife handle

ActiveCN309677515SSurgical bladeKnife blades
1. The name of the design product: handle of scalpel. 2. The use of the design product: handle of scalpel blade. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view.
Owner:SHENZHEN PROPERTY TECH CO LTD

A scalpel blade remover assembly

PCT designated stageWO2025231535A1Incision instrumentsSurgical furnitureKnife bladesScalpel blade
A scalpel blade remover assembly comprising: one or more cartridges, each cartridge including a scalpel blade remover; and a stand having a holder attached to a base, the holder able to hold the one or more cartridges, the base extending both forwardly and rearwardly of the holder, wherein the base allows the holder to move between a first angled position and a second substantially vertical position.
Owner:SMARTSTREAM PTY LTD

Automatic device for disinfecting and cooling caenorhabditis elegans scalpel

The invention discloses an automatic device for disinfecting and cooling a caenorhabditis elegans scalpel. The automatic device comprises a control box, a supporting bottom plate, a supporting stand column, two alcohol lamps, a rotary driving mechanism, an air cooling mechanism and four blade clamping mechanisms. The supporting stand column is rotationally and vertically installed in the center of the supporting bottom plate. The four blade clamping mechanisms are installed at the upper ends of the supporting stand columns. The two alcohol lamps are arranged at the two heating points; the rotation driving mechanism is used for driving the supporting stand column to rotate. And the air cooling mechanism is arranged at the cooling point. According to the automatic device for disinfecting and cooling the scalpel, the supporting stand column can be rotationally driven, so that the point positions where the four blade clamping mechanisms are located are switched at regular time, and finally, the heated and disinfected scalpel blade is automatically fed into the air cooling mechanism to be rapidly cooled, so that the disinfecting efficiency of the scalpel blade is improved; and the scalding danger possibly caused by manual handheld heating can be avoided, and automatic heating and cooling of the surgical blade are achieved.
Owner:NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A +1

Sharps disposal apparatus

An apparatus for the disposal of sharps such as single use needles, scalpel blades and the like comprises a housing 14 having an enclosing wall 18 (Figure 1), an entry aperture 90 in the housing for r
Owner:FRONTIER PLASTICS SOUTH WALES

Scalpel and preparation method of diamond film for scalpel

The invention relates to a preparation method of a diamond film for a scalpel. The preparation method comprises the following steps: selecting and pretreating a scalpel base material; carrying out plasma thinning and sharpening treatment on the blade of the scalpel by adopting integrated equipment which is formed by combining hot filament chemical vapor deposition containing a bias power supply and plasma enhanced chemical vapor deposition; the method comprises the following steps: immersing a scalpel blade into a suspension containing nano-diamond powder, and adsorbing nano-diamond particles on the surface of the scalpel blade through ultrasonic treatment; the scalpel blade is placed in a cavity of the integrated equipment, gas containing a carbon source and hydrogen are introduced, only hot filament chemical vapor deposition is started, and the diamond film is deposited on the surface of the blade. According to the invention, the thickness of the tip area of the blade is 10-50nm, an ultra-sharp tip shape is formed, the surgical cutting precision is greatly improved, and the extreme sharpness is realized.
Owner:INSTITUTE OF MATERIALS & INTELLIGENT MANUFACTURING JIANGXI ACADEMY OF SCIENCES

Multi-functional surgical instrument with attachable surgical instrument bits

Surgical instrument bits mate to a multi-function surgical handle. The surgical instrument bits include an integrated light source, and are disposable or reusable. Surgical instrument bits comprise scalpel blades, skin hooks, retractors, curettes, comedone extractors, forceps, elevators, dissectors, elevators, bone hooks, suture removers, punch biopsy instruments and probes, reflecting devices such as mirrors, trocars and splinter liberators.
Owner:NANO SURGICAL INC

Scalpel blade container

The invention resides in a scalpel blade container for containing removed scalpel blades. The container has a hollow body having an internal space with a first section in which a scalpel blade remover is able to be positioned and a second section in which removed scalpel blades can be contained. A backflow preventer is provided that separates the first section and the second section and allows removed scalpel blades to pass from the first section to the second section but prevents removed scalpel blades from passing from the second section back into the first section.
Owner:SMARTSTREAM PTY LTD

Collecting device special for sharp instruments

The utility model relates to the technical field of medical instruments, in particular to a special sharp instrument collecting device which comprises a collecting box, a storage barrel, a first inlet, a storage box A and a second inlet, box doors are installed on the two sides of the collecting box through hinges, and lock catches are fixed to the centers of one sides of the box doors through bolts. The top of the collecting box penetrates through the storage barrel through a reserved opening, a first inlet and a second inlet are formed in the other side of the top of the collecting box, a placing groove is formed in one side of the top in the collecting box, and a storage box A and a storage box B are arranged at the bottom in the collecting box; ventilation windows are fixed to the bottoms of the two sides of the collecting box through reserved openings and bolts, and filter screens are fixed to one sides of the ventilation windows through bolts. Needle tubes or scalpels are placed in the clamping grooves and matched with blocking of the limiting plates, so that needle heads or scalpel blades fall into the storage barrel to be collected in a centralized mode, and the needle heads or the scalpel blades are prevented from scratching or puncturing the skin.
Owner:GUANGDONG GUANGYE GREEN TECH CO LTD

Introducer Assembly, Drain Placement System, and Related Devices and Methods

An introducer assembly is provided that can install a drain as an outpatient procedure and corresponding method. The assembly includes an elongate introducer having a distal end with a blunt tip and an opposite proximal end enabling user manipulation. The elongate introducer includes a stylet and a sleeve, one of which forms the blunt tip. The sleeve has a canular wall defining a hollow interior and a stylet access opening therethrough at the sleeve proximal end region. The stylet is arranged as a sleeve insert having a handle laterally extending through the stylet access opening while within the sleeve, which can be slidably withdrawn from the sleeve at the distal end region while remaining portions of the elongate introducer are within a wound to be drained. The elongate introducer can include an embedded scalpel blade and retractable sheath for cutting wound entry and exit incisions when installing a drain.
Owner:SURGI RIGHT LLC

Surgical knife blade

1. Name of the designed product: scalpel blade. 2. Use of the designed product: for surgical operation. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view 2.
Owner:THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE (YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE)

Scalpel blade remover

PCT designated stageWO2025164859A1Incision instrumentsDiagnosticsScalpel bladeGeneral surgery
The present invention relates to a scalpel blade remover comprising: an insertion part (100) having an insertion through-hole (110) so that a medical scalpel (M) is inserted therethrough; a guide part (200) coupled perpendicularly to the insertion part (100) at the rear surface of the insertion part (100); a sliding part (300) slidably coupled to the guide part (200) through a coupling part (400) so as to come into contact with a curved connection part (20) and move forward in a first direction when the medical scalpel (M) is inserted into the insertion through-hole (110) and then moved forward in the first direction (D1), and thus a scalpel blade (40) coupled to a scalpel blade coupling part (30) is removed; and a casing part (500) having a front surface to which the insertion part (100) is coupled, and having an inner space formed therein such that the guide part (200) and the sliding part (300) are disposed at the upper portion of the inner space (510) and the scalpel blade (40) removed through the sliding part (300) is stored at the lower portion thereof.
Owner:BAE SUNG HWAN

Method for determining ultrasonic scalpel surgical procedure based on ultrasonic scalpel big data, and data system

The present invention relates to the field of ultrasonic scalpels. Disclosed are a method for determining an ultrasonic scalpel surgical procedure based on ultrasonic scalpel big data, and a data system. The method comprises: acquiring basic information, which comprises type information of pending surgery; querying, in an information system, historical surgical information of the same surgical type, wherein the information system stores interrelated historical surgical type information, intraoperative ultrasonic scalpel blade ID information, and postoperative recovery information; selecting, from the query result, postoperative recovery information conforming to a standard, and using a historical surgical information entry where said postoperative recovery information is located as a reference information entry; on the basis of the obtained reference information entry, matching, in a data set storing intraoperative ultrasonic scalpel blade ID information and used surgical procedure information corresponding to the intraoperative ultrasonic scalpel blade ID information, surgical procedure information corresponding to blade ID information; and performing statistics collection and analysis to determine a surgical procedure. In the present invention, historical surgical information closest to the current pending surgery is matched, and postoperative recovery information is used as a reference entry, so as to more objectively recommend a preferred surgical procedure for the current pending surgery.
Owner:ENSURGE MEDICAL (SUZHOU) CO LTD

Scalpel blade and preparation method thereof

The invention belongs to the field of medical instruments, and discloses a scalpel blade and a preparation method thereof.The scalpel blade is of a three-layer composite structure which sequentially comprises a base layer, a reinforcing layer and a surface functional layer from inside to outside; the base layer is prepared from the following raw materials in parts by weight: 75-80 parts of cubic boron nitride, 15-20 parts of titanium alloy powder, 5-8 parts of tungsten carbide, 3-5 parts of silicon nitride and 1-2 parts of yttrium oxide; the reinforcing layer is prepared from the following raw materials in parts by weight: 8-12 parts of silicon carbide whiskers, 20-25 parts of cubic zirconium oxide, 10-15 parts of strontium titanate, 3-5 parts of molybdenum disilicide and 4-6 parts of zirconium nitride; the surface functional layer is prepared from the following raw materials in parts by weight: 10-15 parts of hydroxyapatite, 5-8 parts of bioactive glass, 3-5 parts of titanium nitride, 2-4 parts of titanium dioxide and 1-2 parts of silver powder; in order to solve the problems that an existing scalpel blade is insufficient in strength, poor in wear resistance and poor in biocompatibility, the strength, the wear resistance and the biocompatibility of the blade are synergistically improved through the design of a three-layer composite structure and optimization of a composite coating.
Owner:HUAIAN BAIZHONG MEDICAL EQUIPMENT CO LTD

Transparent scalpel handle and bayonet with universal blade holder with and without optical lenses

A scalpel handle (100) and bayonet with universal blade holder (110) is disclosed. The scalpel handle and bayonet with universal blade holder can be light transmissible and disposable. The scalpel handle and bayonet with universal blade holder may include one or more integrated lenses (140i) that enable multi-directional and broad dispersion of light to provide surface area for amplification of light to a surgical site and also the surrounding area. The integrated lenses may be convex, concave or flat. One or more illumination sources (135) may be disposed within the body of the scalpel handle and / or the bayonet with universal blade holder. A light pipe (120) may be used to transmit light from the illumination source along the bayonet with universal blade holder. A scalpel blade or other surgical instrument may be affixed to the universal blade holder attached to the bayonet.
Owner:NANO SURGICAL INC

Mammary gland flap dissociation device

The embodiment of the invention discloses a mammary gland flap dissociation device. The mammary gland flap dissociation device comprises a fixing rod, a handle, a first positioning adjusting mechanism, a pressing piece, a second positioning adjusting mechanism and a scalpel blade. Wherein the handle is connected to the fixing rod. The first positioning adjusting mechanism is arranged on the fixing rod. And the pressing sheet is arranged on the first positioning adjusting mechanism. And a positioning structure is arranged on the pressing sheet. And the second positioning adjusting mechanism is arranged on the fixing rod. And the scalpel blade is mounted on the second positioning and adjusting mechanism. In addition, the scalpel blade is located below the same side of the pressing piece. According to the mammary gland flap dissociation device, the thickness of the dissociated flap can be determined according to the actual situation, heat damage is avoided, the length of the scalpel can be adjusted, and the range of the flap needing to be dissociated can be reached.
Owner:杨进

Titanium alloy for ultrasonic scalpel rod, ultrasonic scalpel rod and machining method of ultrasonic scalpel rod

According to the titanium alloy for the ultrasonic knife rod, the ultrasonic knife rod and the machining method of the titanium alloy, the Ti-V-Fe-Al-Nb series titanium alloy is adopted, the content of Fe is controlled to be 0.8%-1.2%, the content of Nb is controlled to be 0.5%-1%, the content of V is controlled to be 8%-10%, the content of Al is controlled to be 3.5%-4.5%, the content of O in the titanium alloy is smaller than 0.12%, and the content of Al in the titanium alloy is smaller than 0.12%. The method comprises the following steps of: performing solution treatment on a cast ingot, performing accumulative ply rolling on the cast ingot to obtain a thin plate, performing linear cutting on the thin plate to obtain a bar, processing the bar into a cutter bar, performing laser-assisted precision cutting to adjust the surface smoothness, performing gradient aging heat treatment, and finally depositing an Ag-TiO coating on the surface, thereby developing a new generation of ultrasonic cutter bar through component fine adjustment and process optimization. And the material has excellent performance and long service life.
Owner:CHANGSHA LEPU SURGICAL MEDICAL INSTRUMENTS CO LTD

A method for precisely obtaining pine wood nematodes from inoculated pine trees

This invention discloses a method for accurately obtaining pine wood nematodes from inoculated pine trees, relating to the field of pest control technology. The method includes the following steps: (1) collecting pine wood nematodes and preparing a nematode suspension; (2) making a 45° oblique cut on the pine bark at the inoculation point using a sterilized scalpel blade, with the wound reaching the phloem; (3) placing defatted cotton into the wound and sealing the bottom of the wound with Parafilm sealing film wrapped around the pine trunk; (4) dripping the nematode suspension onto the defatted cotton using a pipette; (5) at a specific time after inoculation, cutting the pine branches and twigs below the inoculation point to separate and collect the pine wood nematodes, and performing data statistics and analysis. This invention confirms that a method can accurately obtain the vast majority of pine wood nematodes in inoculated pine trees by collecting only branches and twigs within 20 cm below the inoculation point.
Owner:GUANGDONG ACAD OF FORESTRY